当前位置： SCI文献检索 > MICROBIOLOGICAL RESEARCH期刊下所有文献 > The Adh1 gene of the fungus Metarhizium anisopliae is expressed during insect colonization and required for full virulence.

The Adh1 gene of the fungus Metarhizium anisopliae is expressed during insect colonization and required for full virulence.

Abstract：

:Zymography of alcohol dehydrogenase (ADH) activity in the entomopathogenic fungus Metarhizium anisopliae grown under various conditions revealed that micro-aerobic growth was associated with increased ADH activity. The major ADH protein, AdhIp, was purified to homogeneity by affinity chromatography and has an estimated molecular weight of 41kDa and an isoelectric point (pI) of 6.4. Peptide mass fingerprint analysis allowed the identification and cloning of the gene that encodes this protein, Adh1, as annotated in the M. anisopliae genome database. AdhIp is related to the medium-chain dehydrogenase/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family and contains conserved ADH sequence motifs, such as the zinc-containing ADH signature, the FAD/NAD binding domain and amino acid residues that are conserved in most microbial ADHs. Semi-quantitative RT-PCR analysis revealed that Adh1 gene expression occurs at low levels during early Plutella xylostella infection and that the Adh1 gene was primarily expressed at larval death and as mycelia emerge from the insect cuticle before conidiation. Antisense-RNA experiments indicated that NAD(+)-dependent ADH activity was diminished by 20-75% in the transformants, and the transformants that had lower ADH activity showed allyl alcohol resistance, which indicates that reduction in ADH activity also occurs in vivo. Bioassays performed using antisense adh1 transformants, which have lower ADH activity, showed that LC50 values were two to five times higher than the wild-type, indicating that AdhIp is required for full capability of the fungus to penetrate and/or colonize the insect.

journal_name

Microbiol Res

journal_title

Microbiological research

authors

Callejas-Negrete OA,Torres-Guzmán JC,Padilla-Guerrero IE,Esquivel-Naranjo U,Padilla-Ballesteros MF,García-Tapia A,Schrank A,Salazar-Solís E,Gutiérrez-Corona F,González-Hernández GA

doi

10.1016/j.micres.2014.11.006

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

57-67

eissn

0944-5013

issn

1618-0623

pii

S0944-5013(14)00147-5

journal_volume

172

pub_type

杂志文章

在线工具

The Adh1 gene of the fungus Metarhizium anisopliae is expressed during insect colonization and required for full virulence.