Abstract:
:Saccharomyces cerevisiae and Candida shehatae were co-immobilized in an agar sheet which was introduced in an original two-chambered bioreactor asymmetrically fed in a batch mode with a mixture of glucose and xylose in a ratio of 35:15. The two sugars were consumed simultaneously. All glucose was fermented but only 20% of xylose. After incubation, yeast cells recovered from different areas of the agar sheet (close to, called Hi, and distant from, called Ho, the substrate chamber) were cultured as suspended cells in fresh culture medium provided with xylose or the sugar mixture. Xylose utilization by gel released Hi yeasts was significantly delayed compared to the Ho culture. Ethanol consumption by Hi yeasts in the two-substrate medium occurred after glucose exhaustion despite the presence of xylose. The waste medium resulting from incubation of the immobilized-cell structure inhibited xylose utilization by C. shehatae. Our results suggested that batch fermentation most probably favoured this incomplete xylose fermentation.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Lebeau T,Jouenne T,Junter GAdoi
10.1016/j.micres.2006.07.005subject
Has Abstractpub_date
2007-01-01 00:00:00pages
211-8issue
3eissn
0944-5013issn
1618-0623pii
S0944-5013(06)00084-Xjournal_volume
162pub_type
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journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.09.005
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