Abstract：
:Seventy nine isolates of Botrytis cinerea were collected from different host plants and different locations of India and Nepal. All the isolates were identified as B. cinerea based on morphological features and were confirmed using B. cinerea specific primers. Differentiation among the isolates was assessed using morphological, genetic and biochemical approaches. To analyze morphological variability, differences in conidial size, presence or absence of sclerotia and their arrangement were observed. Genetic variability was characterized using RAPD analysis, presence or absence of transposons and mating type genes. Cluster analysis based on RAPD markers was used for defining groups on the basis of geographical region and host. The biochemical approach included determining differences in concentration of oxalic acid and activity of lytic enzymes. All the isolates were categorized into different pathogenic groups on the basis their variable reaction towards chickpea plants. Isolates with higher concentration of oxalic acid and greater activity of lytic enzymes were generally more pathogenic. Pathogenicity was also correlated to transposons. Isolates containing transposa group showed some degree of correlation with pathogenic behavior. However, isolates could not be grouped on the basis of a single approach which provides evidence of their wide diversity and high evolution potential. Sensitivity of sampled isolates was also tested against five botryticides. Most of the isolates from same region were inhibited by a particular fungicide. This feature provided interesting cues and would assist in devising novel and more effective measures for managing the disease.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Kumari S,Tayal P,Sharma E,Kapoor Rdoi
10.1016/j.micres.2014.02.012subject
Has Abstractpub_date
2014-11-01 00:00:00pages
862-72issue
11eissn
0944-5013issn
1618-0623pii
S0944-5013(14)00028-7journal_volume
169pub_type
杂志文章abstract：:Forty Bacillus isolates obtained from maari (used as condiment in Burkina Faso) including 17 B. subtilis, 4 B. circulans, 7 B. pumilus and 6 B. licheniformis were investigated for use as starter cultures in maari production. The isolates were screened by PCR for the sfp gene responsible for the production of the lipop...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.11.005
更新日期：2018-03-01 00:00:00
abstract：:Xanthomonas axonopodis pv. punicae (Xap), the bacterial blight pathogen of pomegranate, incurs substantial loss to yield and reduces export quality of this economically important fruit crop. During infection, the bacterium secretes six non-TAL (Xop) effectors into the pomegranate cells through a specialized type three...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126560
更新日期：2020-11-01 00:00:00
abstract：:One strain of Lactobacillus acidophilus was found to produce a bacteriocin-like substance in the culture filtrate. The substance was produced in a growth-associated manner, showed heat stability at neutral and acidic pH and exhibited antibacterial activity against various species of Lactobacillus including L. acidophi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00190
更新日期：2003-01-01 00:00:00
abstract：:In search of the optimal way to reduce the hazards of environmental contamination by formaldehyde (FD) and methanol the use of unconventional yeasts is proposed as exemplified by the methylotrophic yeast Hansenula polymorpha. In a very simplified environment of a model wastewater solution, H. polymorpha cells were abl...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(00)80002-6
更新日期：2000-03-01 00:00:00
abstract：:The APSES family, comprising of the transcriptional regulators Asm1p, Phd1p, Sok2p, Efg1p, and StuA, is found exclusively in fungi and has been reported to control several cellular processes in these organisms. However, its function in dermatophytes has not yet been completely understood. Here, we generated two null m...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126592
更新日期：2020-12-01 00:00:00
abstract：:Investigating the entire species composition of the microorganisms is crucial to understand their roles in the biogeochemical cycles. Metagenomic DNA was extracted from six intertidal sediment samples along the Liaodong Bay of Bohai Sea, China, and was sequenced which yielded a total of 64,496 high-quality sequences f...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.09.019
更新日期：2014-07-01 00:00:00
abstract：:Actinomadura hibisca P157-2 produces potent antifungal antibiotic pradimicins. To enhance pradimicin production, ACCase from Streptomyces coelicolor and two regulatory genes metK1-sp and afsR-sp from Streptomyces peuticus were overexpressed into A. hibisca using an integration vector pSET152 under the control of the s...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2011.02.007
更新日期：2011-12-20 00:00:00
abstract：:Sponges harbor diverse prokaryotic and eukaryotic microbes. However, the nature of sponge-fungal association and diversity of sponge-derived fungi have barely been addressed. In this study, the cultivation-dependent approach was applied to study fungal diversity in the Hawaiian sponges Gelliodes fibrosa, Haliclona cae...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2007.07.002
更新日期：2009-01-01 00:00:00
abstract：:The survivability of Salmonella cells in popcorn preparation was determined for two distinct cooking methods. The first method used a standard microwave oven. The second method used conventional cooking in a pan. Prior to thermal processing in independent experiments, 12 suspensions in a range between 1x10(3) and 8x10...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.03.010
更新日期：2008-01-01 00:00:00
abstract：:Hydrogenases are the key enzymes for the biological hydrogen production, which can be classified as H(2)-uptake hydrogenase and H(2)-production hydrogenase. The genes encoding a membrane-bound [NiFe]-hydrogenase (MBH), which is mainly responsible for hydrogen uptake, from the photosynthetic bacterium Allochromatium vi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.03.001
更新日期：2009-01-01 00:00:00
abstract：:Tannase producing fungal strains were isolated from different locations including garbages, forests and orchards, etc. The strain giving maximum enzyme yield was identified to be Aspergillus ruber. Enzyme production was studied under solid state fermentation using different tannin rich substrates like ber leaves (Zyzy...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.06.012
更新日期：2007-01-01 00:00:00
abstract：:FtsH proteins are ubiquitous membrane-bound, ATP-dependent metalloproteases of the AAA family. In eubacteria, FtsH is involved in protein quality control under stress conditions. Lactobacillus plantarum is a widespread lactic acid bacterium that is encountered in several fermented food, including dairy products, veget...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2011.07.001
更新日期：2012-04-20 00:00:00
abstract：:Sestrins are highly conserved; stress inducible proteins that help maintain metabolic homeostasis and protect cells under stress conditions. They are up-regulated during stress and influence AMPK and mTOR pathways. Our objective was to find the role of Sestrin protein from Dictyostelium discoideum (Dd), a lower eukary...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.12.006
更新日期：2019-03-01 00:00:00
abstract：:Bacillus subtilis EA-CB0575 is a plant growth-promoting bacterium (PGPB) associated with banana and tomato crops. Root colonization is an important trait for PGPB microorganisms and potentiates the bacterial effect related to the mechanisms of plant growth promotion. Therefore, detection of bacterial colonization of r...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.08.017
更新日期：2018-12-01 00:00:00
abstract：:Staphylococcus saprophyticus is a gram-positive microorganism responsible for urinary tract infections (UTIs). Although some virulence factors are characterized, such as urease, autolysins, adhesins and hemagglutinins, large-scale proteomic studies have not been performed within this species. We performed the characte...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.08.008
更新日期：2018-11-01 00:00:00
abstract：:Colonization of micropropagated sugarcane plants by Gluconacetobacter diazotrophicus and Herbaspirillum sp. was confirmed by a dot-immunoblot assay. In all, a 45-day short-term and 180-day long-term experiments conducted on micropropagated sugarcane plants of Co 86032, a sugar rich popular variety in South India, indi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.08.007
更新日期：2006-01-01 00:00:00
abstract：:Bacteria of the genus Agrobacterium can transfer a portion of their Ti plasmid (T-DNA) in complex with the VirE2 and VirD2 proteins into the plant-cell nucleus and cause it to be integrated in the host-cell chromosomes. The mechanism of T-DNA transfer across the plant-cell membrane and cytoplasm is unknown. The aim of...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.09.012
更新日期：2005-01-01 00:00:00
abstract：:Human interferon alpha2b (IFN-alpha2b) is a pleiotropic cytokine used for the treatment of various cancers. Antibacterial peptide CM4 is a small peptide that can strongly inhibit many kinds of bacteria, fungi, and tumor cells, but it does no harm to normal cells. Here, we describe a protein expression system for the p...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.01.002
更新日期：2010-02-28 00:00:00
abstract：:The genus Pseudomonas is known to comprise a huge diversity of species with the ability to thrive in different habitats, including those considered as extreme environments. In the present study, a psychrotolerant, wide pH tolerant and halotolerant strain of Pseudomonas chlororaphis GBPI_507 (MCC2693), isolated from th...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.04.017
更新日期：2016-09-01 00:00:00
abstract：:Desaturases, key enzymes in the metabolism of fatty acids, regulate the physical and biochemical properties of membranes. They adjust the composition of saturated and unsaturated fatty acids in response to changes in the environmental. We demonstrated the existence of Δ9 desaturase activity in epimastigotes of the Try...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.07.012
更新日期：2017-01-01 00:00:00
abstract：:Non-conventional yeasts are increasingly applied in fermented beverage industry to obtain distinctive products with improved quality. Among these yeasts, Lachancea thermotolerans has multiple features of industrial relevance, especially the production of l(+)-lactic acid (LA), useful for the biological acidification o...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126525
更新日期：2020-09-01 00:00:00
abstract：:The emergence of resistance among fungal phytopathogens poses a biggest threat across the world. Streptomyces are a group of spore-forming Gram + ve bacteria and prolific producers of secondary bioactive metabolites which have been used as biocontrol agents against phytopathogens and also known for plant growth promot...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126478
更新日期：2020-08-01 00:00:00
abstract：:We previously reported that inactivation of a universally conserved dimethyl adenosine transferase (KsgA) attenuates virulence and increases sensitivity to oxidative and osmotic stress in Salmonella Enteritidis. Here, we show a role of KsgA in cell-envelope fitness as a potential mechanism underlying these phenotypes ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.08.009
更新日期：2018-11-01 00:00:00
abstract：:The greater wax moth Galleria mellonella has been increasingly used as a model host to determine Candida albicans virulence and efficacy of antifungal treatment. The G. mellonella lysozyme, similarly to its human counterpart, is a member of the c-type family of lysozymes that exhibits antibacterial and antifungal acti...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.10.003
更新日期：2016-12-01 00:00:00
abstract：:β-lactamase inhibitors are potent synergistic drugs to deteriorate the multidrug-resistant bacteria. Here, we report the β-lactamase inhibitory ability of kalafungin isolated from a marine sponge derived Streptomyces sp. SBRK1. The IC50 value of the kalafungin was calculated as 225.37 ± 1.95 μM against β-lactamase. Th...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126666
更新日期：2021-03-01 00:00:00
abstract：:The methylotrophic yeast, Ogataea thermomethanolica TBRC656, is an attractive host organism for heterologous protein production owing to the availability of protein expression vectors and a genome-editing tool. In this study, we focused on mating-type switching and gene expression in order to elucidate its sexual life...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.126372
更新日期：2020-02-01 00:00:00
abstract：:Classic genome editing tools including ZFN, TALEN, and CRISPR/Cas9 rely on DNA double-strand breaks for genome editing. To prevent the potential hazard caused by double-strand breaks (DSBs), a series of single base editing tools that convert cytidine (C) to thymine (T) without DSBs have been developed extensively in m...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.03.007
更新日期：2019-01-01 00:00:00
abstract：:The SAP6 gene (without signal sequence) encoding Metschnikowia reukaufii acid protease was amplified by PCR and fused to the expression vector pET-24a(+). The carboxy-terminal 6x His-tagged recombinant acid protease (rSAP6) was expressed from pET-24a(+)SAP6-6His in Escherichia coli BL21 (DE3) and purified with affinit...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.08.003
更新日期：2010-03-31 00:00:00
abstract：:To evaluate the roles of blaIMP and blaTEM genes in the resistance of Serratia marcescens against beta-lactams and to find the spreading ways of these genes, 19 clinical isolates of imipenem-resistant Serratia marcescens were analyzed. Six strains bore blaIMP and blaTEM genes on a single plasmid, as confirmed by trans...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.06.005
更新日期：2007-01-01 00:00:00
abstract：:Biofilms are the most widely distributed and successful microbial modes of life. The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress. Bacteria living in cold environments...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.09.010
更新日期：2019-01-01 00:00:00
