Abstract：
:Human interferon alpha2b (IFN-alpha2b) is a pleiotropic cytokine used for the treatment of various cancers. Antibacterial peptide CM4 is a small peptide that can strongly inhibit many kinds of bacteria, fungi, and tumor cells, but it does no harm to normal cells. Here, we describe a protein expression system for the production of IFN-alpha2b/CM4 fusion protein in insoluble form in Escherichia coli, coupled to an efficient dialysis refolding and histidine-tag purification protocol. The expressed IFN-alpha2b/CM4 fusion protein not only displays significantly improved antitumor activity, but also retains the antibacterial-antifungal activity of CM4.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Li NN,Liu P,Chen SJ,Lin QP,Zhou LF,Zhang SQdoi
10.1016/j.micres.2009.01.002subject
Has Abstractpub_date
2010-02-28 00:00:00pages
116-21issue
2eissn
0944-5013issn
1618-0623pii
S0944-5013(09)00003-2journal_volume
165pub_type
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