Characterization of bacterial diversity in two aerated lagoons of a wastewater treatment plant using PCR-DGGE analysis.

Abstract:

:Aerated lagoons are commonly used for domestic and industrial wastewater treatment due to their low cost and minimal need of operational requirements. However, little information is known regarding microbial communities that inhabit these ecosystems. In this study, a 16S-DGGE approach was used to estimate bacterial diversity and to monitor community changes in two aerated lagoons from a wastewater treatment plant receiving urban and industrial effluents. Pronounced shifts between bacterial communities collected in winter-spring and summer-autumn months were detected. Temperature, dissolved oxygen (DO) and pH were the variables that most influenced the bacterial communities. Phylogenetic affiliation of predominant members was assessed by the determination of the 16S rDNA sequence of correspondent bands. Affiliations to Cytophaga-Flexibacter-Bacteroides (CFB) group, Firmicutes, and beta- and epsilon-proteobacteria were found.

journal_name

Microbiol Res

journal_title

Microbiological research

authors

Moura A,Tacão M,Henriques I,Dias J,Ferreira P,Correia A

doi

10.1016/j.micres.2007.06.005

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

560-9

issue

5

eissn

0944-5013

issn

1618-0623

pii

S0944-5013(07)00085-7

journal_volume

164

pub_type

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