Bacterial chitin binding proteins show differential substrate binding and synergy with chitinases.


:Glycosyl hydrolase (GH) family 18 chitinases (Chi) and family 33 chitin binding proteins (CBPs) from Bacillus thuringiensis serovar kurstaki (BtChi and BtCBP), B. licheniformis DSM13 (BliChi and BliCBP) and Serratia proteamaculans 568 (SpChiB and SpCBP21) were used to study the efficiency and synergistic action of BtChi, BliChi and SpChiB individually with BtCBP, BliCBP or SpCBP21. Chitinase assay revealed that only BtChi and SpChiB showed synergism in hydrolysis of chitin, while there was no increase in products generated by BliChi, in the presence of the three above mentioned CBPs. This suggests that some (specific) CBPs are able to exert a synergistic effect on (specific) chitinases. A mutant of BliChi, designated as BliGH, was constructed by deleting the C-terminal fibronectin III (FnIII) and carbohydrate binding module 5 (CBM5) to assess the contribution of FnIII and CBM5 domains in the synergistic interactions of GH18 chitinases with CBPs. Chitinase assay with BliGH revealed that the accessory domains play a major role in making BliChi an efficient enzyme. We studied binding of BtCBP and BliCBP to α- and β-chitin. The BtCBP, BliCBP or SpCBP21 did not act synergistically with chitinases in hydrolysis of the chitin, interspersed with other polymers, present in fungal cell walls.


Microbiol Res


Microbiological research


Manjeet K,Purushotham P,Neeraja C,Podile AR




Has Abstract


2013-08-25 00:00:00














  • Survivability of Salmonella cells in popcorn after microwave oven and conventional cooking.

    abstract::The survivability of Salmonella cells in popcorn preparation was determined for two distinct cooking methods. The first method used a standard microwave oven. The second method used conventional cooking in a pan. Prior to thermal processing in independent experiments, 12 suspensions in a range between 1x10(3) and 8x10...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Anaya I,Aguirrezabal A,Ventura M,Comellas L,Agut M

    更新日期:2008-01-01 00:00:00

  • Identification, cloning, expression, and purification of Francisella lpp3: an immunogenic lipoprotein.

    abstract::The severe and fatal human disease, tularemia, results from infection with the Gram-negative pathogen Francisella tularensis. Identification of surface outer membrane proteins, specifically lipoproteins, has been of interest for vaccine development and understanding the initiation of disease. We sought to identify Fra...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Parra MC,Shaffer SA,Hajjar AM,Gallis BM,Hager A,Goodlett DR,Guina T,Miller S,Collins CM

    更新日期:2010-09-20 00:00:00

  • PCR-identification of Dunaliella salina (Volvocales, Chlorophyta) and its growth characteristics.

    abstract::The saline pond microalga, Dunaliella salina (Dunal) Teod. maintained in De Walne's (basal) medium under laboratory conditions was confirmed by amplifying the chromosomal DNA of the microalga by PCR with specific primers MA1 and MA2. Seaweed extracts obtained from Sargassum wightii and Ulva lactuca were amended separa...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Raja R,Hema Iswarya S,Balasubramanyam D,Rengasamy R

    更新日期:2007-01-01 00:00:00

  • Macrobrachium rosenbergii cathepsin L: molecular characterization and gene expression in response to viral and bacterial infections.

    abstract::Cathepsin L (MrCathL) was identified from a constructed cDNA library of freshwater prawn Macrobrachium rosenbergii. MrCathL full-length cDNA is 1161 base pairs (bp) with an ORF of 1026bp which encodes a polypeptide of 342 amino acid (aa) long. The eukaryotic cysteine proteases, histidine and asparagine active site res...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Arockiaraj J,Gnanam AJ,Muthukrishnan D,Thirumalai MK,Pasupuleti M,Milton J,Kasi M

    更新日期:2013-11-07 00:00:00

  • Suppression of damping-off in maize seedlings by Pseudomonas corrugata.

    abstract::Two strains of Pseudomonas corrugata, (1 and 7), isolated from subtropical and temperate soils in Sikkim Himalaya, respectively, were subjected to Petri-dish as well as plant-based bioassay to examine their potential for disease suppression against three major pathogens of maize. A mixture of Pythium ultimum, P. arrhe...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Pandey A,Palni LM,Hebbar KP

    更新日期:2001-01-01 00:00:00

  • Strain-level diversity of secondary metabolism in the biocontrol species Aneurinibacillus migulanus.

    abstract::Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a sing...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Alenezi FN,Rekik I,Bełka M,Ibrahim AF,Luptakova L,Jaspars M,Woodward S,Belbahri L

    更新日期:2016-01-01 00:00:00

  • Colonization factors of enterotoxigenic E. coli (ETEC) from residents of northern Egypt.

    abstract::Infection caused by enterotoxigenic Escherichia coli (ETEC) poses a serious health problem to children in developing countries. Colonization of the small intestinal mucosa by ETEC strains is mediated by antigenically specific fimbriae, also known as colonization factor antigens (CFA). The importance of this study aris...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Oyofo BA,el-Etr SH,Wasfy MO,Peruski L,Kay B,Mansour M,Campbell JR,Svennerholm AM,Churilla AM,Murphy JR

    更新日期:1995-11-01 00:00:00

  • Partial purification and characterization of the bacteriocin produced by Lactobacillus acidophilus YIT 0154.

    abstract::One strain of Lactobacillus acidophilus was found to produce a bacteriocin-like substance in the culture filtrate. The substance was produced in a growth-associated manner, showed heat stability at neutral and acidic pH and exhibited antibacterial activity against various species of Lactobacillus including L. acidophi...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Yamato M,Ozaki K,Ota F

    更新日期:2003-01-01 00:00:00

  • Maize rhizosphere in Sichuan, China, hosts plant growth promoting Burkholderia cepacia with phosphate solubilizing and antifungal abilities.

    abstract::Plant growth-promoting rhizobacteria promote plant growth by direct and indirect mechanisms. We isolated twelve bacterial strains showing different degrees of phosphate solubilizing activity from maize rhizosphere. Four isolates solubilized over 300 μg mL⁻¹ phosphate from insoluble Ca₃(PO₄)₂, with isolate SCAUK0330 so...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Zhao K,Penttinen P,Zhang X,Ao X,Liu M,Yu X,Chen Q

    更新日期:2014-01-20 00:00:00

  • Physiologically distinct subpopulations formed in Escherichia coli cultures in response to heat shock.

    abstract::Bacteria can form heterogeneous populations containing phenotypic variants of genetically identical cells. The heterogeneity of populations can be considered a bet-hedging strategy allowing adaptation to unknown environmental changes - at least some individual subpopulations or cells might be able to withstand future ...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Bruhn-Olszewska B,Szczepaniak P,Matuszewska E,Kuczyńska-Wiśnik D,Stojowska-Swędrzyńska K,Moruno Algara M,Laskowska E

    更新日期:2018-04-01 00:00:00

  • A cost effective cultivation medium for biocalcification of Bacillus pasteurii KCTC 3558 and its effect on cement cubes properties.

    abstract::Application of carbonate precipitation induced by Bacillus pasteurii for improving some properties of cement has been reported. However, it is not yet successful in commercial scale due to the high cost of cultivation medium. This is the first report on the application of effluent from chicken manure bio-gas plant, a ...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Yoosathaporn S,Tiangburanatham P,Bovonsombut S,Chaipanich A,Pathom-Aree W

    更新日期:2016-05-01 00:00:00

  • Antibacterial activity of Lactobacillus species against Vibrio species.

    abstract::Forty-one Lactobacillus strains were tested for antagonistic activity against nine strains of Vibrio. L. plantarum and L. casei were the most effective, and L. brevis was the least effective in inhibiting the growth of Vibrio species. L. gasseri and L. helveticus strains showed higher activity, while L. reuteri and L....

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Koga T,Mizobe T,Takumi K

    更新日期:1998-11-01 00:00:00

  • Influence of salinity on methanogenesis and associated microflora in tropical rice soils.

    abstract::In a laboratory incubation study, methane (CH4) production in two saline soils and a nonsaline soil sample was investigated under flooded conditions. Mean CH4 production was remarkable (630.86 ng CH4/g) in nonsaline alluvial soil, but low (12.97 ng CH4/g) in acid sulfate saline (Pokkali) soil which was attributed to t...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Pattnaik P,Mishra SR,Bharati K,Mohanty SR,Sethunathan N,Adhya TK

    更新日期:2000-09-01 00:00:00

  • The new flagella-associated collagen-like proteins ClpB and ClpC of Bacillus amyloliquefaciens FZB42 are involved in bacterial motility.

    abstract::Collagen-like proteins (CLPs) share the distinctive Gly-X-Thr repeating amino acid sequence of animal collagens, and contain N- and C-terminal domain making a collagen-like structure in Bacillus amyloliquefaciens FZB42, a plant growth-promoting rhizobacterium. Our previous study demonstrated that CLPs play important r...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Zhao X,Wang R,Shang Q,Hao H,Li Y,Zhang Y,Guo Z,Wang Y,Xie Z

    更新日期:2016-03-01 00:00:00

  • Genes encoding FAD-binding proteins in Volvariella volvacea exhibit differential expression in homokaryons and heterokaryons.

    abstract::Flavin adenine dinucleotide (FAD)-binding proteins play a vital role in energy transfer and utilization during fungal growth and mycelia aggregation. We sequenced the genome of Volvariella volvacea, an economically important edible fungus, and discovered 41 genes encoding FAD-binding proteins. Gene expression profiles...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Meng L,Yan J,Xie B,Li Y,Chen B,Liu S,Li D,Yang Z,Zeng X,Deng Y,Jiang Y

    更新日期:2013-10-01 00:00:00

  • Phosphatidylcholine affects the secretion of the alkaline phosphatase PhoA in Pseudomonas strains.

    abstract::Pseudomonas aeruginosa ATCC 27853 and Pseudomonas sp. 593 use the phosphatidylcholine synthase pathway (Pcs-pathway) for the biosynthesis of phosphatidylcholine (PC). Both bacterial strains contain the phoA and lapA genes encoding alkaline phosphatases (ALP) and display strong ALP activities. The PhoA and LapA enzymes...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Liu X,Long D,You H,Yang D,Zhou S,Zhang S,Li M,He M,Xiong M,Wang X

    更新日期:2016-11-01 00:00:00

  • A phenazine-1-carboxylic acid producing polyextremophilic Pseudomonas chlororaphis (MCC2693) strain, isolated from mountain ecosystem, possesses biocontrol and plant growth promotion abilities.

    abstract::The genus Pseudomonas is known to comprise a huge diversity of species with the ability to thrive in different habitats, including those considered as extreme environments. In the present study, a psychrotolerant, wide pH tolerant and halotolerant strain of Pseudomonas chlororaphis GBPI_507 (MCC2693), isolated from th...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Jain R,Pandey A

    更新日期:2016-09-01 00:00:00

  • Ethanol fermentation of mahula (Madhuca latifolia L.) flowers using free and immobilized yeast Saccharomyces cerevisiae.

    abstract::There is a growing interest to find alternate bioresources for production of ethanol, apart from cane/sugar beet molasses and starchy crops like sweet sorghum, cassava and sweet potato. Mahula (Madhuca latifolia L.) is a forest tree abundantly available in the Indian subcontinent and its flowers are very rich in ferme...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Swain MR,Kar S,Sahoo AK,Ray RC

    更新日期:2007-01-01 00:00:00

  • Characterization of Ffh of Mycobacterium tuberculosis and its interaction with 4.5S RNA.

    abstract::Signal recognition particle (SRP) mediates targeting of proteins to appropriate cellular compartments, which is an important process in all living organisms. In prokaryotes, SRP consists of Ffh, a protein, and 4.5S RNA that recognizes signal peptide emerging from ribosomes. The SRP (Ffh) of one the most successful int...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Palaniyandi K,Veerasamy M,Narayanan S

    更新日期:2012-10-12 00:00:00

  • Could FlhF be a key element that controls Campylobacter jejuni flagella biosynthesis in the initial assembly stage?

    abstract::The disordered arrangement of flagella biosynthetic genes, combined with a simplified regulatory mechanism, has made elucidating the process of Campylobacter jejuni flagellation difficult. FlhF is a recently identified element that controls the assembly of the flagella, although its function mechanism and regulatory p...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Ren F,Lei T,Song Z,Yu T,Li Q,Huang J,Jiao XA

    更新日期:2018-03-01 00:00:00

  • Microbial communities and their potential for degradation of dissolved organic carbon in cryoconite hole environments of Himalaya and Antarctica.

    abstract::Cryoconite holes (cylindrical melt-holes on the glacier surface) are important hydrological and biological systems within glacial environments that support diverse microbial communities and biogeochemical processes. This study describes retrievable heterotrophic microbes in cryoconite hole water from three geographica...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Sanyal A,Antony R,Samui G,Thamban M

    更新日期:2018-03-01 00:00:00

  • Enhancement of pradimicin production in Actinomadura hibisca P157-2 by metabolic engineering.

    abstract::Actinomadura hibisca P157-2 produces potent antifungal antibiotic pradimicins. To enhance pradimicin production, ACCase from Streptomyces coelicolor and two regulatory genes metK1-sp and afsR-sp from Streptomyces peuticus were overexpressed into A. hibisca using an integration vector pSET152 under the control of the s...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Paudel S,Lee HC,Kim BS,Sohng JK

    更新日期:2011-12-20 00:00:00

  • Galleria mellonella lysozyme induces apoptotic changes in Candida albicans cells.

    abstract::The greater wax moth Galleria mellonella has been increasingly used as a model host to determine Candida albicans virulence and efficacy of antifungal treatment. The G. mellonella lysozyme, similarly to its human counterpart, is a member of the c-type family of lysozymes that exhibits antibacterial and antifungal acti...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Sowa-Jasiłek A,Zdybicka-Barabas A,Stączek S,Wydrych J,Skrzypiec K,Mak P,Deryło K,Tchórzewski M,Cytryńska M

    更新日期:2016-12-01 00:00:00

  • Isolation and initial characterization of the tellurite reducing moderately halophilic bacterium, Salinicoccus sp. strain QW6.

    abstract::Among the 49 strains of moderately halophilic bacteria isolated from the salty environments of Iran, a Gram-positive coccus designated as strain QW6 showed high capacity in the removal of toxic oxyanions of tellurium in a wide range of culture medium factors including pH (5.5-10.5), temperature (25-45 degrees C), vari...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Amoozegar MA,Ashengroph M,Malekzadeh F,Reza Razavi M,Naddaf S,Kabiri M

    更新日期:2008-01-01 00:00:00

  • Identification of putative effector genes and their transcripts in three strains related to 'Candidatus Phytoplasma aurantifolia'.

    abstract::Molecular mechanisms underlying phytoplasma interactions with host plants are largely unknown. In this study attempts were made to identify effectors of three phytoplasma strains related to 'Ca. P. aurantifolia', crotalaria phyllody (CrP), faba bean phyllody (FBP), and witches' broom disease of lime (WBDL), using info...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Anabestani A,Izadpanah K,Abbà S,Galetto L,Ghorbani A,Palmano S,Siampour M,Veratti F,Marzachì C

    更新日期:2017-06-01 00:00:00

  • Molecular identification of Azospirillum spp.: Limitations of 16S rRNA and qualities of rpoD as genetic markers.

    abstract::Since their discovery, plant-growth promoting rhizobacteria from the genus Azospirillum have been subjected to intensive research due to their biotechnological potential as crop inoculants. Phylogenetic analysis of Azospirillum spp. is carried out by 16S rRNA sequencing almost exclusively, but inconsistencies and low ...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Maroniche GA,García JE,Salcedo F,Creus CM

    更新日期:2017-01-01 00:00:00

  • 2D-PAGE examination of mRNA populations from Penicillium freii mutants deficient in xanthomegnin biosynthesis.

    abstract::Penicillium freii (Lund and Frisvad 1994) mutants deficient in the synthesis of xanthomegnin were isolated. In vitro translated mRNA populations from selected radiation induced mutants and naturally occurring P. freii strains not able to produce xanthomegnin were examined by 2-dimensional polyacrylamide gel electropho...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Nicolaisen M,Sandal T,Frisvad JC,Rossen L

    更新日期:1996-08-01 00:00:00

  • Metagenomic cloning and characterization of Na⁺ transporters from Huamachi Salt Lake in China.

    abstract::Moderately halophilic bacteria are a kind of extreme environment microorganism that can tolerate moderate salt concentrations ranging from 0.5M to 2.5M. Here, via a metagenomic library screen, we identified four putative Na(+) transporters, designated H7-Nha, H16-Mppe, H19-Cap and H35-Mrp, from moderately halophilic c...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Gao M,Tao L,Chen S

    更新日期:2013-02-22 00:00:00

  • Potential of selected lactic acid bacteria to produce food compatible antifungal metabolites.

    abstract::The aim of this study was to assess the potential of lactic acid bacteria to inhibit the outgrowth of some common food-spoiling fungi. Culture supernatants of 17 Lactic acid bacterial strains as well as of three commercial probiotic cultures were evaluated for antifungal activity using an agar-diffusion method. The me...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: De Muynck C,Leroy AI,De Maeseneire S,Arnaut F,Soetaert W,Vandamme EJ

    更新日期:2004-01-01 00:00:00

  • Molecular characterization of Panton-Valentine leukocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolates in Egypt.

    abstract::Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) expressing Panton-Valentine leukocidin (PVL) have been characterized worldwide, but it has not been done in Egypt. In this study, we analyzed the molecular characteristics of PVL+CA-MRSA in Egypt, compared their genetic patterns with that of PVL+...

    journal_title:Microbiological research

    pub_type: 杂志文章


    authors: Enany S,Yaoita E,Yoshida Y,Enany M,Yamamoto T

    更新日期:2010-02-28 00:00:00