Abstract：
:Glycosyl hydrolase (GH) family 18 chitinases (Chi) and family 33 chitin binding proteins (CBPs) from Bacillus thuringiensis serovar kurstaki (BtChi and BtCBP), B. licheniformis DSM13 (BliChi and BliCBP) and Serratia proteamaculans 568 (SpChiB and SpCBP21) were used to study the efficiency and synergistic action of BtChi, BliChi and SpChiB individually with BtCBP, BliCBP or SpCBP21. Chitinase assay revealed that only BtChi and SpChiB showed synergism in hydrolysis of chitin, while there was no increase in products generated by BliChi, in the presence of the three above mentioned CBPs. This suggests that some (specific) CBPs are able to exert a synergistic effect on (specific) chitinases. A mutant of BliChi, designated as BliGH, was constructed by deleting the C-terminal fibronectin III (FnIII) and carbohydrate binding module 5 (CBM5) to assess the contribution of FnIII and CBM5 domains in the synergistic interactions of GH18 chitinases with CBPs. Chitinase assay with BliGH revealed that the accessory domains play a major role in making BliChi an efficient enzyme. We studied binding of BtCBP and BliCBP to α- and β-chitin. The BtCBP, BliCBP or SpCBP21 did not act synergistically with chitinases in hydrolysis of the chitin, interspersed with other polymers, present in fungal cell walls.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Manjeet K,Purushotham P,Neeraja C,Podile ARdoi
10.1016/j.micres.2013.01.006subject
Has Abstractpub_date
2013-08-25 00:00:00pages
461-8issue
7eissn
0944-5013issn
1618-0623pii
S0944-5013(13)00007-4journal_volume
168pub_type
杂志文章abstract：:The aim of the present study, performed on typical Argiudolls in a natural reserve with little or no anthropic impact, was to characterize the fungous biomineralizing process of calcium oxalate crystals in organic horizons of the soil. The chosen sites possessed different plant cover, identified as acacia woods and gr...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00083
更新日期：2001-01-01 00:00:00
abstract：:Pseudomonas aeruginosa is one of the most common pathogens associated with nosocomial infections and a great concern to immunocompromised individuals especially in the cases of cystic fibrosis, AIDS and burn wounds. The pathogenicity of P. aeruginosa is largely directed by the quorum sensing (QS) system. Hence, QS may...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.05.001
更新日期：2019-09-01 00:00:00
abstract：:A microbial consortium reducing high level of perchlorate was developed and in a fed batch bioreactor using acetate as substrate perchlorate was reduced at 0.25 g/g vss. day. Under stable performance, the microbial community structure of the reactor was analyzed through molecular and phenotypic methods. The diversity ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.04.011
更新日期：2015-08-01 00:00:00
abstract：:Bitespiramycin (biotechnological spiramycin, Bsm) is a new 16-membered macrolide antibiotic produced by Streptomyces spiramyceticus WSJ-1 integrated exogenous genes. The gene cluster for Bsm biosynthesis consists of two parts: spiramycin biosynthetic gene cluster (92 kb) and two exogenous genes including 4"-O-isovaler...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126532
更新日期：2020-11-01 00:00:00
abstract：:Seventy nine isolates of Botrytis cinerea were collected from different host plants and different locations of India and Nepal. All the isolates were identified as B. cinerea based on morphological features and were confirmed using B. cinerea specific primers. Differentiation among the isolates was assessed using morp...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2014.02.012
更新日期：2014-11-01 00:00:00
abstract：:Bacterial carbonate precipitation has implications in geological processes and important biotechnological applications. Bacteria capable of precipitating carbonates have been isolated from different calcium carbonate deposits (speleothems) in caves, soil, freshwater and seawater around the world. However, the diversit...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.09.006
更新日期：2016-01-01 00:00:00
abstract：:Microorganisms often suffer from oxidative stress created from nutrient starvation and environmental changes. Thioredoxin (Trx) and glutathione (GSH) pathways are believed critical in related protective functions. The roles of Trx in improving abiotic stress resistance in Trichoderma reesei are still unclear. In this ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.01.005
更新日期：2015-02-01 00:00:00
abstract：:DrrC is a DNA-binding protein of Streptomyces peucetius that provides self-resistance against daunorubicin, the antibiotic produced by the organism. DrrC was expressed in E.coli and purified by using N-terminal MBP-tag which retained DNA-binding property in spite of the tag. Mobility shift assay confirmed the interact...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.05.002
更新日期：2017-09-01 00:00:00
abstract：:Pulmonary hemorrhage is an increasing cause of death of leptospirosis patients. Bacterial collagenase has been shown to be involved in lung hemorrhage induced by various infectious agents. According to Leptospira whole genome study, colA, a gene suggested to code for bacterial collagenase has been identified. We inves...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2012.12.005
更新日期：2013-06-12 00:00:00
abstract：:Forty Bacillus isolates obtained from maari (used as condiment in Burkina Faso) including 17 B. subtilis, 4 B. circulans, 7 B. pumilus and 6 B. licheniformis were investigated for use as starter cultures in maari production. The isolates were screened by PCR for the sfp gene responsible for the production of the lipop...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.11.005
更新日期：2018-03-01 00:00:00
abstract：:Although the use of crop-associated bacteria as biological control agents of fungal diseases has gained increasing interest, the biotechnological potential of forest tree-associated microbes and their natural products has scarcely been investigated. The objective of this study was to identify bacteria or bacterial pro...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126440
更新日期：2020-05-01 00:00:00
abstract：:Annual ryegrass is a fast-growing cool-season grass broadly present in the Portuguese "montado", a typically Mediterranean agro-forestry-pastoral ecosystem. A culture-dependent approach was used to investigate natural associations of this crop with potentially beneficial bacteria, aiming to identify strains suitable f...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.12.010
更新日期：2014-09-01 00:00:00
abstract：:We have cloned a laccase gene fragment isolated from a Trametes versicolor strain in Korea. It showed high similarity in nucleotide sequences when compared with other fungal laccases. TNT (2,4,6-trinitrotoluene), a widely used explosive, was transformed rapidly by T. versicolor. When TNT and its catabolic intermediate...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.12.001
更新日期：2006-01-01 00:00:00
abstract：:The aim of this study was to assess the potential of lactic acid bacteria to inhibit the outgrowth of some common food-spoiling fungi. Culture supernatants of 17 Lactic acid bacterial strains as well as of three commercial probiotic cultures were evaluated for antifungal activity using an agar-diffusion method. The me...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.07.002
更新日期：2004-01-01 00:00:00
abstract：:The genus Pseudomonas is known to comprise a huge diversity of species with the ability to thrive in different habitats, including those considered as extreme environments. In the present study, a psychrotolerant, wide pH tolerant and halotolerant strain of Pseudomonas chlororaphis GBPI_507 (MCC2693), isolated from th...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.04.017
更新日期：2016-09-01 00:00:00
abstract：:Forty-one Lactobacillus strains were tested for antagonistic activity against nine strains of Vibrio. L. plantarum and L. casei were the most effective, and L. brevis was the least effective in inhibiting the growth of Vibrio species. L. gasseri and L. helveticus strains showed higher activity, while L. reuteri and L....
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(98)80011-6
更新日期：1998-11-01 00:00:00
abstract：:Sestrins are highly conserved; stress inducible proteins that help maintain metabolic homeostasis and protect cells under stress conditions. They are up-regulated during stress and influence AMPK and mTOR pathways. Our objective was to find the role of Sestrin protein from Dictyostelium discoideum (Dd), a lower eukary...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.12.006
更新日期：2019-03-01 00:00:00
abstract：:A strain of Fusarium moniliforme isolated from a tropical mangrove ecosystem near Mumbai, India and deposited in the National Collection of Industrial Microorganisms (NCIM) as F. moniliforme NCIM 1276. The organism produced a single extracellular polygalacturonase (PG I) [EC 3.2.1.15] at pH 5 and a single pectate lyas...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.06.002
更新日期：2004-01-01 00:00:00
abstract：:The classical salicylaldehyde dehydrogenases found in naphthalene-degrading bacteria are denoted as NahF. In addition to NahF, NahV, and its corresponding gene nahV, were found here in multiple naphthalene-degrading bacteria isolated from industrial wastewater polluted with polycyclic aromatic hydrocarbons (PAHs). In ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2011.01.003
更新日期：2011-12-20 00:00:00
abstract：:Endophytic bacteria are the plant beneficial bacteria that thrive inside plants and can improve plant growth under normal and challenging conditions. They can benefit host plants directly by improving plant nutrient uptake and by modulating growth and stress related phytohormones. Indirectly, endophytic bacteria can i...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2019.02.001
更新日期：2019-04-01 00:00:00
abstract：:Actinomycetes have been isolated from three Brazilian tropical soils. The dispersion and differential centrifugation procedure revealed count values 1.5 to 5.0 times greater than those obtained by the conventional dilution plate technique for all soils and media tested. Eighteen strains, promising for biotechnological...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(01)80007-0
更新日期：2001-03-01 00:00:00
abstract：:Hirsutella rhossiliensis OWVT-1 has substantial potential as a biocontrol agent against plant-parasitic nematodes. Serine proteases have emerged as a potentially useful factor in the nematode-fungus interactions. When grown in liquid culture with the nematode Panagrellus redivivus as the sole nitrogen source, an extra...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.01.003
更新日期：2009-01-01 00:00:00
abstract：:For a long a time programmed cell death was thought to be a unique characteristic of higher eukaryotes, but evidence has accumulated showing that programmed cell death is a universal phenomenon in all life forms. Many different types of bacterial programmed cell death systems have been identified, rivalling the eukary...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2017.11.016
更新日期：2018-03-01 00:00:00
abstract：:Cyclophosphamide (CP) is the most commonly used drug in autoimmune disease, cancer, blood and marrow transplantation. Recent data revealed that therapy efficacy of CP is gut microbiota-dependent. So, it is very important to understand how CP affects intestinal microbiota and immune function. In this study, the effects...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2014.11.002
更新日期：2015-02-01 00:00:00
abstract：:Freeze-drying technology has been widely considered for decades as a suitable technique to preserve microorganisms. However, protective agents must be added prior to freeze drying to improve the survival and storage stability of the bacteria. The objective of our study was to evaluate the effect of a new protectant me...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126454
更新日期：2020-06-01 00:00:00
abstract：:In Salmonella Typhimurium (S. Typhimurium), lipopolysaccharide (LPS) anchored on the bacterial outer membrane is a major immune stimulus that can broadly activate immune cells and induce innate immune responses. wzxE is involved in bacterial LPS biosynthesis but has rarely been reported in Salmonella; wzxE encodes a f...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126502
更新日期：2020-09-01 00:00:00
abstract：:The pectinolytic enzyme from the solid-state culture of Rhizopus oryzae NBRC 4707 was purified to homogeneity by column chromatography on CM-Toyopearl 650 M and hydroxylapatite. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 31,000 and was reduced to 29,700 after treat...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.01.001
更新日期：2004-01-01 00:00:00
abstract：:Mycobacterium tuberculosis Rv2140c is a function unknown conserved phosphatidylethanolamine-binding protein (PEBP), homologous to Raf kinase inhibitor protein (RKIP) in human beings. To delineate its function, we heterologously expressed Rv2140c in a non-pathogenic M. smegmatis. Quantitative phosphoproteomic analysis ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126615
更新日期：2021-01-01 00:00:00
abstract：:Yeast colonies isolated from vineyard and cellar substrates were analysed in the present study. Yeast species assessment was carried out by amplification and digestion of a region of the ribosomal RNA gene repeat unit. Saccharomyces strains were also characterised using mitochondrial DNA restriction analysis. Oxidativ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00163
更新日期：2002-01-01 00:00:00
abstract：:During meiosis in Saccharomyces cerevisiae four daughter cells, called spores, are generated within the boundaries of the mother cell. This cell differentiation process requires de novo synthesis of prospore membranes (PSMs), which are the precursors of the spore plasma membranes. Assembly of these membranes is initia...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1078/0944-5013-00194
更新日期：2003-01-01 00:00:00
