Abstract：
:The pectinolytic enzyme from the solid-state culture of Rhizopus oryzae NBRC 4707 was purified to homogeneity by column chromatography on CM-Toyopearl 650 M and hydroxylapatite. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 31,000 and was reduced to 29,700 after treatment with endoglycosidase H. Maximal activity was observed near pH 4.5 at 45 degrees C. The enzyme was shown to be endopolygalacturonase, as judged from the formation of oligogalacturonides as its reaction products. The addition of purified enzyme, as expected, enhanced the formation of lactic acid and ethanol in potato pulp grown with R. oryzae.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Saito K,Takakuwa N,Oda Ydoi
10.1016/j.micres.2004.01.001subject
Has Abstractpub_date
2004-01-01 00:00:00pages
83-6issue
1eissn
0944-5013issn
1618-0623pii
S0944-5013(04)00003-5journal_volume
159pub_type
杂志文章abstract：:The emergence of resistance among fungal phytopathogens poses a biggest threat across the world. Streptomyces are a group of spore-forming Gram + ve bacteria and prolific producers of secondary bioactive metabolites which have been used as biocontrol agents against phytopathogens and also known for plant growth promot...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126478
更新日期：2020-08-01 00:00:00
abstract：:Bacterial utilization of monomers is recognized as an important step in the biogeochemical cycling of organic matter. In this study we have compared the heterotrophic activity of bacterial communities from different micro-habitats within a salt marsh environment (Ria de Aveiro, Portugal) in order to establish spatial ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.02.001
更新日期：2005-01-01 00:00:00
abstract：:The saline pond microalga, Dunaliella salina (Dunal) Teod. maintained in De Walne's (basal) medium under laboratory conditions was confirmed by amplifying the chromosomal DNA of the microalga by PCR with specific primers MA1 and MA2. Seaweed extracts obtained from Sargassum wightii and Ulva lactuca were amended separa...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.03.006
更新日期：2007-01-01 00:00:00
abstract：:Actinomycetes are virtually unlimited sources of novel compounds with many therapeutic applications and hold a prominent position due to their diversity and proven ability to produce novel bioactive compounds. There are more than 22,000 known microbial secondary metabolites, 70% of which are produced by actinomycetes,...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2012.06.005
更新日期：2012-12-20 00:00:00
abstract：:Twenty-nine rhizobial isolates from root nodules of the wild Legumes Astragalus, Lespedeza and Hedysarum growing in the northwestern region of China, were characterized by numerical taxonomy, RFLP and sequencing of PCR-amplified 16S rDNA genes, and cross-nodulation with selected Legume species. Based on the results fr...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.09.005
更新日期：2008-01-01 00:00:00
abstract：:There are a lot of bacterial and eukaryotic DNA repair gene homologs among sequenced archaeal genomes but there is little information about DNA repair mechanisms and the interaction of involved repair proteins. In order to study DNA repair mechanisms in the third domain of life, we studied these processes in the model...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.01.012
更新日期：2015-04-01 00:00:00
abstract：:Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) expressing Panton-Valentine leukocidin (PVL) have been characterized worldwide, but it has not been done in Egypt. In this study, we analyzed the molecular characteristics of PVL+CA-MRSA in Egypt, compared their genetic patterns with that of PVL+...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.03.005
更新日期：2010-02-28 00:00:00
abstract：:Seventy nine isolates of Botrytis cinerea were collected from different host plants and different locations of India and Nepal. All the isolates were identified as B. cinerea based on morphological features and were confirmed using B. cinerea specific primers. Differentiation among the isolates was assessed using morp...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2014.02.012
更新日期：2014-11-01 00:00:00
abstract：:Signal recognition particle (SRP) mediates targeting of proteins to appropriate cellular compartments, which is an important process in all living organisms. In prokaryotes, SRP consists of Ffh, a protein, and 4.5S RNA that recognizes signal peptide emerging from ribosomes. The SRP (Ffh) of one the most successful int...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2012.03.002
更新日期：2012-10-12 00:00:00
abstract：:The emergent pathogen Candida glabrata differs from other yeasts because it assimilates only two sugars, glucose and the disaccharide trehalose. Since rapid identification tests are based on the ability of this yeast to rapidly hydrolyze trehalose, in this work a biochemical and molecular characterization of trehalose...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.06.008
更新日期：2015-10-01 00:00:00
abstract：:We have examined the capacity of Halobacterium halobium and Sulfolobus solfataricus to withstand the lethal effect of hydrogen peroxide and N-methyl-N'-nitro-N-nitrosoguanidine [corrected]. We tested a variety of pretreatment regimens with both mutagens and all failed to elicit an inducible response to the lethal eff...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(97)80037-7
更新日期：1997-09-01 00:00:00
abstract：:Salmonella Pullorum is a pathogen specific to birds that can cause Pullorum disease in young chickens and lead to considerable economic losses in the poultry industry. During transmission and infection, S. Pullorum will encounter various environmental stresses and host defenses. The stringent response is an important ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126685
更新日期：2021-01-05 00:00:00
abstract：:The classical salicylaldehyde dehydrogenases found in naphthalene-degrading bacteria are denoted as NahF. In addition to NahF, NahV, and its corresponding gene nahV, were found here in multiple naphthalene-degrading bacteria isolated from industrial wastewater polluted with polycyclic aromatic hydrocarbons (PAHs). In ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2011.01.003
更新日期：2011-12-20 00:00:00
abstract：:Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease (JD) in animals, has also been linked with Crohn's disease in human beings. Lack of indigenous diagnostics and vaccine hampered control of JD in India. Designing effective control strategies require thorough understanding of ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.03.007
更新日期：2010-02-28 00:00:00
abstract：:Bacteria of the genus Agrobacterium can transfer a portion of their Ti plasmid (T-DNA) in complex with the VirE2 and VirD2 proteins into the plant-cell nucleus and cause it to be integrated in the host-cell chromosomes. The mechanism of T-DNA transfer across the plant-cell membrane and cytoplasm is unknown. The aim of...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.09.012
更新日期：2005-01-01 00:00:00
abstract：:The APSES family, comprising of the transcriptional regulators Asm1p, Phd1p, Sok2p, Efg1p, and StuA, is found exclusively in fungi and has been reported to control several cellular processes in these organisms. However, its function in dermatophytes has not yet been completely understood. Here, we generated two null m...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126592
更新日期：2020-12-01 00:00:00
abstract：:Microorganisms often suffer from oxidative stress created from nutrient starvation and environmental changes. Thioredoxin (Trx) and glutathione (GSH) pathways are believed critical in related protective functions. The roles of Trx in improving abiotic stress resistance in Trichoderma reesei are still unclear. In this ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.01.005
更新日期：2015-02-01 00:00:00
abstract：:Molecular mechanisms underlying phytoplasma interactions with host plants are largely unknown. In this study attempts were made to identify effectors of three phytoplasma strains related to 'Ca. P. aurantifolia', crotalaria phyllody (CrP), faba bean phyllody (FBP), and witches' broom disease of lime (WBDL), using info...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.03.001
更新日期：2017-06-01 00:00:00
abstract：:Glycosyl hydrolase (GH) family 18 chitinases (Chi) and family 33 chitin binding proteins (CBPs) from Bacillus thuringiensis serovar kurstaki (BtChi and BtCBP), B. licheniformis DSM13 (BliChi and BliCBP) and Serratia proteamaculans 568 (SpChiB and SpCBP21) were used to study the efficiency and synergistic action of BtC...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.01.006
更新日期：2013-08-25 00:00:00
abstract：:Sponges harbor diverse prokaryotic and eukaryotic microbes. However, the nature of sponge-fungal association and diversity of sponge-derived fungi have barely been addressed. In this study, the cultivation-dependent approach was applied to study fungal diversity in the Hawaiian sponges Gelliodes fibrosa, Haliclona cae...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2007.07.002
更新日期：2009-01-01 00:00:00
abstract：:Yeast colonies isolated from vineyard and cellar substrates were analysed in the present study. Yeast species assessment was carried out by amplification and digestion of a region of the ribosomal RNA gene repeat unit. Saccharomyces strains were also characterised using mitochondrial DNA restriction analysis. Oxidativ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00163
更新日期：2002-01-01 00:00:00
abstract：:The genus Pseudomonas is known to comprise a huge diversity of species with the ability to thrive in different habitats, including those considered as extreme environments. In the present study, a psychrotolerant, wide pH tolerant and halotolerant strain of Pseudomonas chlororaphis GBPI_507 (MCC2693), isolated from th...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.04.017
更新日期：2016-09-01 00:00:00
abstract：:Cyclophosphamide (CP) is the most commonly used drug in autoimmune disease, cancer, blood and marrow transplantation. Recent data revealed that therapy efficacy of CP is gut microbiota-dependent. So, it is very important to understand how CP affects intestinal microbiota and immune function. In this study, the effects...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2014.11.002
更新日期：2015-02-01 00:00:00
abstract：:The wild-type yeast Saccharomyces cerevisiae (S. cerevisiae) is able to export less than 1 percent of the protein to be secreted. The reasons for retention of most of the secretory proteins on the cell surface of S. cerevisiae are unknown. Recently, temperature-sensitive (ts) mutants of S. cerevisiae showing an overse...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00067
更新日期：2001-01-01 00:00:00
abstract：:In a laboratory incubation study, methane (CH4) production in two saline soils and a nonsaline soil sample was investigated under flooded conditions. Mean CH4 production was remarkable (630.86 ng CH4/g) in nonsaline alluvial soil, but low (12.97 ng CH4/g) in acid sulfate saline (Pokkali) soil which was attributed to t...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(00)80035-X
更新日期：2000-09-01 00:00:00
abstract：:Although it is known that oxalic acid provides a selective advantage to the secreting microbe our understanding of how this acid is biosynthesized remains incomplete. This study reports the identification, cloning, and partial characterization of the oxalic acid biosynthetic enzyme from the animal bacterial pathogen, ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.11.002
更新日期：2011-10-20 00:00:00
abstract：:Cathepsin L (MrCathL) was identified from a constructed cDNA library of freshwater prawn Macrobrachium rosenbergii. MrCathL full-length cDNA is 1161 base pairs (bp) with an ORF of 1026bp which encodes a polypeptide of 342 amino acid (aa) long. The eukaryotic cysteine proteases, histidine and asparagine active site res...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.04.007
更新日期：2013-11-07 00:00:00
abstract：:Metal tolerance has been found to vary among Acidithiobacillus ferrooxidans strains and this can impact the efficiency of biomining practices. To explain observed strain variability for differences in metal tolerance we examined the effects of Cu(2+) and Ni(2+) concentrations (1-200 mM) on cytoplasmic membrane propert...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.03.004
更新日期：2011-03-20 00:00:00
abstract：:Although reductive soil disinfestation (RSD) is increasingly used for the control of soil-borne diseases, its impact on the soil microbial community during and after RSD remains poorly understood. MiSeq pyrosequencing, real-time PCR and denaturing gradient gel electrophoresis were performed to investigate the changes ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.08.004
更新日期：2015-12-01 00:00:00
abstract：:Increased intestinal absorption of oxalate causes hyperoxaluria, a major risk factor for kidney stone disease. Intestinal colonization of recombinant probiotic bacteria expressing oxalate-degrading gene (OxdC) is an effective therapeutic option for recurrent calcium oxalate (CaOx) stone disease. Therefore, we aimed to...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.06.009
更新日期：2018-10-01 00:00:00
