Abstract:
:The wild-type yeast Saccharomyces cerevisiae (S. cerevisiae) is able to export less than 1 percent of the protein to be secreted. The reasons for retention of most of the secretory proteins on the cell surface of S. cerevisiae are unknown. Recently, temperature-sensitive (ts) mutants of S. cerevisiae showing an oversecretion phenotype were isolated. In order to study the influence of the mitochondrial genome status on protein export in yeast cells, we have isolated several types of respiratory impaired mitochondrial mutants of either the parental S. cerevisiae strain or their derivative ts protein-overexporting mutants. In this paper we demonstrate by quantitative analyses of exported proteins and by SDS-PAGE analysis that protein overexport in ts mutants requires mitochondrial genome integrity and function.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Alexandar I,Venkov P,Del Giudice A,Wolf K,Massardo DR,Del Giudice Ldoi
10.1078/0944-5013-00067subject
Has Abstractpub_date
2001-01-01 00:00:00pages
9-12issue
1eissn
0944-5013issn
1618-0623pii
S0944-5013(04)70002-6journal_volume
156pub_type
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