Protein overexport in a Saccharomyces cerevisiae mutant depends on mitochondrial genome integrity and function.

Abstract:

:The wild-type yeast Saccharomyces cerevisiae (S. cerevisiae) is able to export less than 1 percent of the protein to be secreted. The reasons for retention of most of the secretory proteins on the cell surface of S. cerevisiae are unknown. Recently, temperature-sensitive (ts) mutants of S. cerevisiae showing an oversecretion phenotype were isolated. In order to study the influence of the mitochondrial genome status on protein export in yeast cells, we have isolated several types of respiratory impaired mitochondrial mutants of either the parental S. cerevisiae strain or their derivative ts protein-overexporting mutants. In this paper we demonstrate by quantitative analyses of exported proteins and by SDS-PAGE analysis that protein overexport in ts mutants requires mitochondrial genome integrity and function.

journal_name

Microbiol Res

journal_title

Microbiological research

authors

Alexandar I,Venkov P,Del Giudice A,Wolf K,Massardo DR,Del Giudice L

doi

10.1078/0944-5013-00067

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

9-12

issue

1

eissn

0944-5013

issn

1618-0623

pii

S0944-5013(04)70002-6

journal_volume

156

pub_type

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