Abstract:
:The emergent pathogen Candida glabrata differs from other yeasts because it assimilates only two sugars, glucose and the disaccharide trehalose. Since rapid identification tests are based on the ability of this yeast to rapidly hydrolyze trehalose, in this work a biochemical and molecular characterization of trehalose catabolism by this yeast was performed. Our results show that C. glabrata consumes and ferments trehalose, with parameters similar to those observed during glucose fermentation. The presence of glucose in the medium during exponential growth on trehalose revealed extracellular hydrolysis of the sugar by a cell surface acid trehalase with a pH optimum of 4.4. Approximately ∼30% of the total enzymatic activity is secreted into the medium during growth on trehalose or glycerol. The secreted enzyme shows an apparent molecular mass of 275 kDa in its native form, but denaturant gel electrophoresis revealed a protein with ∼130 kDa, which due to its migration pattern and strong binding to concanavalin A, indicates that it is probably a dimeric glycoprotein. The secreted acid trehalase shows high affinity and activity for trehalose, with Km and Vmax values of 3.4 mM and 80 U (mg protein)(-1), respectively. Cloning of the CgATH1 gene (CAGLOK05137g) from de C. glabrata genome, a gene showing high homology to fungal acid trehalases, allowed trehalose fermentation after heterologous expression in Saccharomyces cerevisiae.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Zilli DM,Lopes RG,Alves SL Jr,Barros LM,Miletti LC,Stambuk BUdoi
10.1016/j.micres.2015.06.008subject
Has Abstractpub_date
2015-10-01 00:00:00pages
12-9eissn
0944-5013issn
1618-0623pii
S0944-5013(15)00116-0journal_volume
179pub_type
杂志文章abstract::Quorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic AHLs [N-...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2013.04.011
更新日期:2013-11-07 00:00:00
abstract::Fusaric acid (FA) is a fungal metabolite produced by several Fusarium species responsible for wilts and root rot diseases of a great variety of plants. Bacillus spp. and Pseudomonas spp. have been considered as promising biocontrol agents against phytopathogenic Fusarium spp., however it has been demonstrated that FA ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2017.09.008
更新日期:2018-01-01 00:00:00
abstract::Given the current trend towards reducing the use of chemical controls in agriculture, microbial resources such as plant endophytes are being intensively investigated for traits that are conducive to plant protection. Among the various important target pathogens, Fusarium graminearum is a fungal pathogen of cereal crop...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126650
更新日期:2021-02-01 00:00:00
abstract::The study of biofilm function, structure and microbial interactions might help to improve our understanding of biofilm wastewater treatment processes. However, few reports specifically address the influence of interactions within multispecies biofilms on microbial activity and biofilm composition. Thus, the relationsh...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2010.08.005
更新日期:2011-09-20 00:00:00
abstract::Three classes of cell wall-defective mutants of the unicellular green alga Chlamydomonas reinhardtii have been described in the literature differing with respect to the amounts of cell wall material and its attachment to the plasma membrane, respectively. We have compared the production of the chaotrope-soluble cell w...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/S0944-5013(97)80012-2
更新日期:1997-07-01 00:00:00
abstract::Long-term continuous cropping has led to epidemic of bacterial wilt disease in Southern China. Bacterial wilt disease is caused by Ralstonia solanacearum and difficult to control. In order to control bacterial wilt, rice hull biochar was applied to soil with different doses (0, 7.5, 15, 30 and 45 t ha-1) in a field tr...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2019.126373
更新日期:2020-01-01 00:00:00
abstract::Cathepsin L (MrCathL) was identified from a constructed cDNA library of freshwater prawn Macrobrachium rosenbergii. MrCathL full-length cDNA is 1161 base pairs (bp) with an ORF of 1026bp which encodes a polypeptide of 342 amino acid (aa) long. The eukaryotic cysteine proteases, histidine and asparagine active site res...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2013.04.007
更新日期:2013-11-07 00:00:00
abstract::Extracellular proteases from 8 Antarctic psychrotolerant Pseudomonas sp. strains were purified and characterised. All of them are neutral metalloproteases, have an apparent molecular mass of 45kDa, optimal activity at 40 degrees C and pH 7-9, retaining significant activity at pH 5-11. With the exception of P96-18, whi...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2004.03.001
更新日期:2004-01-01 00:00:00
abstract::Extended spectrum beta-lactamases (ESBLs) usually associated with multiple drug resistance, including beta-lactam and non-beta-lactam antibiotics. This resistance can cause Limitation in the choice of drugs appropriate for using in clinical practice, especially in life-threatening infections. In this study we aimed to...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2004.10.001
更新日期:2005-01-01 00:00:00
abstract::Chaperone DnaK and its co-chaperone DnaJ plays various essential roles such as in assisting in the folding of nascent peptides, preventing protein aggregation and maintaining cellular protein homeostasis. Global transcriptional changes in vivo associated with deletion of dnaKJ were monitored using DNA microarray to el...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2016.03.001
更新日期:2016-05-01 00:00:00
abstract::Although it is known that oxalic acid provides a selective advantage to the secreting microbe our understanding of how this acid is biosynthesized remains incomplete. This study reports the identification, cloning, and partial characterization of the oxalic acid biosynthetic enzyme from the animal bacterial pathogen, ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2010.11.002
更新日期:2011-10-20 00:00:00
abstract::The pectinolytic enzyme from the solid-state culture of Rhizopus oryzae NBRC 4707 was purified to homogeneity by column chromatography on CM-Toyopearl 650 M and hydroxylapatite. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 31,000 and was reduced to 29,700 after treat...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2004.01.001
更新日期:2004-01-01 00:00:00
abstract::In Salmonella Typhimurium (S. Typhimurium), lipopolysaccharide (LPS) anchored on the bacterial outer membrane is a major immune stimulus that can broadly activate immune cells and induce innate immune responses. wzxE is involved in bacterial LPS biosynthesis but has rarely been reported in Salmonella; wzxE encodes a f...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126502
更新日期:2020-09-01 00:00:00
abstract::There are a lot of bacterial and eukaryotic DNA repair gene homologs among sequenced archaeal genomes but there is little information about DNA repair mechanisms and the interaction of involved repair proteins. In order to study DNA repair mechanisms in the third domain of life, we studied these processes in the model...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.01.012
更新日期:2015-04-01 00:00:00
abstract::Sestrins are highly conserved; stress inducible proteins that help maintain metabolic homeostasis and protect cells under stress conditions. They are up-regulated during stress and influence AMPK and mTOR pathways. Our objective was to find the role of Sestrin protein from Dictyostelium discoideum (Dd), a lower eukary...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2018.12.006
更新日期:2019-03-01 00:00:00
abstract::Seeds are reservoirs of beneficial and harmful microorganism that modulates plant growth and health. Here, we access seed to seedling bacteriome assembly modified by seed-disinfection and the underlined effect over maize germination performance and root-seedlings microbial colonization. Seed-disinfection was performed...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126643
更新日期:2021-02-01 00:00:00
abstract::The endophytic bacteria were isolated from coffee roots and seeds in Vietnam and identified with 16S rDNA sequencing as belonging to the Actinobacteria, Firmicutes and Proteobacteria phyla with the Nocardia, Bacillus and Burkholderia as dominant genera, respectively. Out of the thirty genera recovered from Coffea cane...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126613
更新日期:2021-01-01 00:00:00
abstract::The operation of wastewater treatment technologies depends on a combination of physical, chemical and biological factors. Microorganisms present in wastewater treatment plants play essential roles in the degradation and removal of organic waste and xenobiotic pollutants. Several microorganisms have been used in comple...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2016.08.006
更新日期:2016-11-01 00:00:00
abstract::Hydrogenases are the key enzymes for the biological hydrogen production, which can be classified as H(2)-uptake hydrogenase and H(2)-production hydrogenase. The genes encoding a membrane-bound [NiFe]-hydrogenase (MBH), which is mainly responsible for hydrogen uptake, from the photosynthetic bacterium Allochromatium vi...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2009.03.001
更新日期:2009-01-01 00:00:00
abstract::There is a growing interest to find alternate bioresources for production of ethanol, apart from cane/sugar beet molasses and starchy crops like sweet sorghum, cassava and sweet potato. Mahula (Madhuca latifolia L.) is a forest tree abundantly available in the Indian subcontinent and its flowers are very rich in ferme...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.01.009
更新日期:2007-01-01 00:00:00
abstract::A mercury-resistant plasmid of pTMJ212 which was able to shuttle between Acidithiobacillus caldus and Escherichia coli was constructed by inserting the mercury resistant determinants, the mer operon of Acidithiobacillus ferrooxidans, into the IncQ plasmid of pJRD215. pTMJ212 was transferred from Escherichia coli into ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2010.10.003
更新日期:2011-10-20 00:00:00
abstract::The community and Cd-resistance of endophytic fungi from roots of Salix variegata Franch. collected from the water-level-fluctuation zone of Three Gorges Reservoir Region, China, were investigated. A total of 53 strains were isolated and identified to 13 morphotaxa, in which Chromosporium, Fusarium and Gonatobotrys we...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.03.013
更新日期:2015-07-01 00:00:00
abstract::Prolonged cold storage of raw milks favors the growth of psychrotrophs, which produce heat-resistant exoenzymes of considerable spoilage potential; the bacterial proteases and lipases affect raw milk quality; among them phospholipases (PLs) may target the milk fat globule. More importantly, bacterial PLs are key virul...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2008.07.001
更新日期:2010-01-01 00:00:00
abstract::Central southern Mexico contains highly diverse legumes. In this study, nodule-associated bacteria (NAB) were isolated from wild legume nodules and from nodules on Phaseolus vulgaris plants used as a plant-trap in soils from the same areas as the wild legumes. The bacteria were identified through the 16S rRNA gene seq...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126522
更新日期:2020-10-01 00:00:00
abstract::Biofilms are the most widely distributed and successful microbial modes of life. The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress. Bacteria living in cold environments...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2018.09.010
更新日期:2019-01-01 00:00:00
abstract::Cryoconite holes (cylindrical melt-holes on the glacier surface) are important hydrological and biological systems within glacial environments that support diverse microbial communities and biogeochemical processes. This study describes retrievable heterotrophic microbes in cryoconite hole water from three geographica...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2018.01.004
更新日期:2018-03-01 00:00:00
abstract::To evaluate the roles of blaIMP and blaTEM genes in the resistance of Serratia marcescens against beta-lactams and to find the spreading ways of these genes, 19 clinical isolates of imipenem-resistant Serratia marcescens were analyzed. Six strains bore blaIMP and blaTEM genes on a single plasmid, as confirmed by trans...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.06.005
更新日期:2007-01-01 00:00:00
abstract::Twenty-nine staphylococcal isolates from different clinical samples were tested for slime production: phenotypic characterization was carried out using Christensen test (tube test) and Congo red agar plate test (CRA plate test), while the presence and expression of icaA and icaD genes were evaluated by real-time PCR. ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2007.04.004
更新日期:2009-01-01 00:00:00
abstract::Microorganisms often suffer from oxidative stress created from nutrient starvation and environmental changes. Thioredoxin (Trx) and glutathione (GSH) pathways are believed critical in related protective functions. The roles of Trx in improving abiotic stress resistance in Trichoderma reesei are still unclear. In this ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.01.005
更新日期:2015-02-01 00:00:00
abstract::The use of biocontrol strains recently has become a popular alternative to conventional chemical treatments. A set of bacteria isolated from sugar beet rhizosphere and from roots and shoots of apple and walnut were evaluated for their potential to control sugar beet seedling damping-off caused by R. solani AG-4 and AG...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2016.06.011
更新日期:2016-11-01 00:00:00