Abstract:
:We have examined the capacity of Halobacterium halobium and Sulfolobus solfataricus to withstand the lethal effect of hydrogen peroxide and N-methyl-N'-nitro-N-nitrosoguanidine [corrected]. We tested a variety of pretreatment regimens with both mutagens and all failed to elicit an inducible response to the lethal effects of those compounds in either organism. We have observed AP endonuclease activity in protein extracts from both organisms. In addition, S. solfataricus extracts contain activities that remove 3-methyl-adenine and O(6)-methyl-guanine from methylated DNA. This is the first report of these DNA repair activities in any member of the Archaea.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Prail CA,Taylor WDdoi
10.1016/s0944-5013(97)80037-7subject
Has Abstractpub_date
1997-09-01 00:00:00pages
257-60issue
3eissn
0944-5013issn
1618-0623journal_volume
152pub_type
杂志文章abstract::Actinomadura hibisca P157-2 produces potent antifungal antibiotic pradimicins. To enhance pradimicin production, ACCase from Streptomyces coelicolor and two regulatory genes metK1-sp and afsR-sp from Streptomyces peuticus were overexpressed into A. hibisca using an integration vector pSET152 under the control of the s...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2011.02.007
更新日期:2011-12-20 00:00:00
abstract::The progression of life in all forms is not only dependent on agricultural and food security but also on the soil characteristics. The dynamic nature of soil is a direct manifestation of soil microbes, bio-mineralization, and synergistic co-evolution with plants. With the increase in world's population the demand for ...
journal_title:Microbiological research
pub_type: 杂志文章,评审
doi:10.1016/j.micres.2017.08.016
更新日期:2018-01-01 00:00:00
abstract::Yeast colonies isolated from vineyard and cellar substrates were analysed in the present study. Yeast species assessment was carried out by amplification and digestion of a region of the ribosomal RNA gene repeat unit. Saccharomyces strains were also characterised using mitochondrial DNA restriction analysis. Oxidativ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1078/0944-5013-00163
更新日期:2002-01-01 00:00:00
abstract::The aim of this study was to assess the potential of lactic acid bacteria to inhibit the outgrowth of some common food-spoiling fungi. Culture supernatants of 17 Lactic acid bacterial strains as well as of three commercial probiotic cultures were evaluated for antifungal activity using an agar-diffusion method. The me...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2004.07.002
更新日期:2004-01-01 00:00:00
abstract::Seeds are reservoirs of beneficial and harmful microorganism that modulates plant growth and health. Here, we access seed to seedling bacteriome assembly modified by seed-disinfection and the underlined effect over maize germination performance and root-seedlings microbial colonization. Seed-disinfection was performed...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126643
更新日期:2021-02-01 00:00:00
abstract::Although reductive soil disinfestation (RSD) is increasingly used for the control of soil-borne diseases, its impact on the soil microbial community during and after RSD remains poorly understood. MiSeq pyrosequencing, real-time PCR and denaturing gradient gel electrophoresis were performed to investigate the changes ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.08.004
更新日期:2015-12-01 00:00:00
abstract::A microbial consortium reducing high level of perchlorate was developed and in a fed batch bioreactor using acetate as substrate perchlorate was reduced at 0.25 g/g vss. day. Under stable performance, the microbial community structure of the reactor was analyzed through molecular and phenotypic methods. The diversity ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.04.011
更新日期:2015-08-01 00:00:00
abstract::Zymography of alcohol dehydrogenase (ADH) activity in the entomopathogenic fungus Metarhizium anisopliae grown under various conditions revealed that micro-aerobic growth was associated with increased ADH activity. The major ADH protein, AdhIp, was purified to homogeneity by affinity chromatography and has an estimate...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2014.11.006
更新日期:2015-03-01 00:00:00
abstract::Bitespiramycin (biotechnological spiramycin, Bsm) is a new 16-membered macrolide antibiotic produced by Streptomyces spiramyceticus WSJ-1 integrated exogenous genes. The gene cluster for Bsm biosynthesis consists of two parts: spiramycin biosynthetic gene cluster (92 kb) and two exogenous genes including 4"-O-isovaler...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126532
更新日期:2020-11-01 00:00:00
abstract::"Bryndza" is a traditional Slovak dairy product (type of soft cheese) made from sheep cheese which was ripened for 14 days. Because its manufacture, transporting and/or storing represent conditions which facilitate contamination, the effect of enterocin CCM4231 in "bryndza" was investigated with the aim to reduce the ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1078/0944-5013-00078
更新日期:2001-01-01 00:00:00
abstract::The pectinolytic enzyme from the solid-state culture of Rhizopus oryzae NBRC 4707 was purified to homogeneity by column chromatography on CM-Toyopearl 650 M and hydroxylapatite. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 31,000 and was reduced to 29,700 after treat...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2004.01.001
更新日期:2004-01-01 00:00:00
abstract::Classic genome editing tools including ZFN, TALEN, and CRISPR/Cas9 rely on DNA double-strand breaks for genome editing. To prevent the potential hazard caused by double-strand breaks (DSBs), a series of single base editing tools that convert cytidine (C) to thymine (T) without DSBs have been developed extensively in m...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2019.03.007
更新日期:2019-01-01 00:00:00
abstract::Penicillium freii (Lund and Frisvad 1994) mutants deficient in the synthesis of xanthomegnin were isolated. In vitro translated mRNA populations from selected radiation induced mutants and naturally occurring P. freii strains not able to produce xanthomegnin were examined by 2-dimensional polyacrylamide gel electropho...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/S0944-5013(96)80026-7
更新日期:1996-08-01 00:00:00
abstract::Lactobacillus rhamnosus DSM 14870 and Lactobacillus gasseri DSM 14869 were previously isolated from the vaginal epithelial cells (VEC) of healthy women and selected for the development of the vaginal EcoVag® probiotic capsules. EcoVag® was subsequently shown to provide long-term cure and reduce relapse of bacterial va...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2017.08.003
更新日期:2017-12-01 00:00:00
abstract::The organophosphorus hydrolase (OPH) of Nocardia sp. strain B-1 is capable of hydrolyzing organophosphate insecticides such as coumaphos and parathion. The 40,000 dalton OPH and the responsible gene (termed adpB) have been previously isolated and described. OPH activity in Nocardia strain B-1 is spontaneously lost at ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/s0944-5013(98)80003-7
更新日期:1998-11-01 00:00:00
abstract::The functions of two long-chain fatty acid CoA ligase genes (facl) in crude oil-degrading Geobacillus thermodenitrificans NG80-2 were characterized. Facl1 and Facl2 encoded by GTNG_0892 and GTNG_1447 were expressed in Escherichia coli and purified as His-tagged fusion proteins. Both enzymes utilized a broad range of f...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2012.05.001
更新日期:2012-12-20 00:00:00
abstract::In recent years, many studies have shown that phytochemicals exert their antibacterial activity through different mechanisms of action, such as damage to the bacterial membrane and suppression of virulence factors, including inhibition of the activity of enzymes and toxins, and bacterial biofilm formation. In this rev...
journal_title:Microbiological research
pub_type: 杂志文章,评审
doi:10.1016/j.micres.2016.12.003
更新日期:2017-03-01 00:00:00
abstract::Sestrins are highly conserved; stress inducible proteins that help maintain metabolic homeostasis and protect cells under stress conditions. They are up-regulated during stress and influence AMPK and mTOR pathways. Our objective was to find the role of Sestrin protein from Dictyostelium discoideum (Dd), a lower eukary...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2018.12.006
更新日期:2019-03-01 00:00:00
abstract::Actinomycetes have been isolated from three Brazilian tropical soils. The dispersion and differential centrifugation procedure revealed count values 1.5 to 5.0 times greater than those obtained by the conventional dilution plate technique for all soils and media tested. Eighteen strains, promising for biotechnological...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/S0944-5013(01)80007-0
更新日期:2001-03-01 00:00:00
abstract::Bacteria can form heterogeneous populations containing phenotypic variants of genetically identical cells. The heterogeneity of populations can be considered a bet-hedging strategy allowing adaptation to unknown environmental changes - at least some individual subpopulations or cells might be able to withstand future ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2018.02.002
更新日期:2018-04-01 00:00:00
abstract::Biofilm formation of Group A Streptococcus (GAS) is recognized as an important virulent determinant. The present study reports the antibiofilm potential of seaweed (Gracilaria gracilis) surface associated Bacillus subtilis against GAS. Purification revealed 2,4-Di-tert-butyl-phenol (DTBP) as the active principle. DTBP...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2016.05.010
更新日期:2016-10-01 00:00:00
abstract::The inhibitory effect of onion oil against the growth of various isolates of bacteria representing Gram-positive (4 isolates) and Gram-negative (4 isolates) species were studied. Results show that onion oil was highly active against all Gram-positive bacteria tested and only one isolate (Klebsiella pneumoniae) of Gram...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/S0944-5013(11)80052-2
更新日期:1995-05-01 00:00:00
abstract::In present work we described, for the first time, the phylogenic structure of the microbial community in a shallow freshwater lake (Hawk Island Lake, located in the Lower Peninsula of the State of Michigan, U.S.A.) after one season (four times during May to August 2007) of CuSO₄ treatment for algae growth control. The...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2014.08.008
更新日期:2015-01-01 00:00:00
abstract::Arbuscular Mycorrhizal Fungi (AMF) live in symbiosis with most crop plants and represent essential elements of soil fertility and plant nutrition and productivity, facilitating soil mineral nutrient uptake and protecting plants from biotic and abiotic stresses. These beneficial services may be mediated by the dense an...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.11.012
更新日期:2016-02-01 00:00:00
abstract::The survivability of Salmonella cells in popcorn preparation was determined for two distinct cooking methods. The first method used a standard microwave oven. The second method used conventional cooking in a pan. Prior to thermal processing in independent experiments, 12 suspensions in a range between 1x10(3) and 8x10...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.03.010
更新日期:2008-01-01 00:00:00
abstract::Central southern Mexico contains highly diverse legumes. In this study, nodule-associated bacteria (NAB) were isolated from wild legume nodules and from nodules on Phaseolus vulgaris plants used as a plant-trap in soils from the same areas as the wild legumes. The bacteria were identified through the 16S rRNA gene seq...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126522
更新日期:2020-10-01 00:00:00
abstract::Prolonged cold storage of raw milks favors the growth of psychrotrophs, which produce heat-resistant exoenzymes of considerable spoilage potential; the bacterial proteases and lipases affect raw milk quality; among them phospholipases (PLs) may target the milk fat globule. More importantly, bacterial PLs are key virul...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2008.07.001
更新日期:2010-01-01 00:00:00
abstract::Iron-sulfur ([Fe-S]) cluster is an essential cofactor of proteins involved in various physiological processes including cellular defense against oxidative stress. In Xanthomonas campestris pv. campestris (Xcc), IscR plays a negative role in regulation of the transcription of [Fe-S] assembly genes, iscR-sufBCDS. The ex...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2014.08.004
更新日期:2015-01-01 00:00:00
abstract::The pathogenicity of a new China variety of Metarhizium anisopliae (M. anisopliae var. dcjhyium) against the subterranean termite Odontotermes formosanus and the effect of the fungal fermented solution on hemolymph intracellular calcium were studied in laboratory. Conidia from the M. anisopliae var. dcjhyium were high...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.11.009
更新日期:2009-01-01 00:00:00
abstract::Actinomycetes are virtually unlimited sources of novel compounds with many therapeutic applications and hold a prominent position due to their diversity and proven ability to produce novel bioactive compounds. There are more than 22,000 known microbial secondary metabolites, 70% of which are produced by actinomycetes,...
journal_title:Microbiological research
pub_type: 杂志文章,评审
doi:10.1016/j.micres.2012.06.005
更新日期:2012-12-20 00:00:00