Responses of Halobacterium halobium and Sulfolobus solfataricus to hydrogen peroxide and N-methyl-N'-nitro-N-nitrosoguanidine [correction of N-methyl-N-nitrosoguanidine] exposure.

Abstract:

:We have examined the capacity of Halobacterium halobium and Sulfolobus solfataricus to withstand the lethal effect of hydrogen peroxide and N-methyl-N'-nitro-N-nitrosoguanidine [corrected]. We tested a variety of pretreatment regimens with both mutagens and all failed to elicit an inducible response to the lethal effects of those compounds in either organism. We have observed AP endonuclease activity in protein extracts from both organisms. In addition, S. solfataricus extracts contain activities that remove 3-methyl-adenine and O(6)-methyl-guanine from methylated DNA. This is the first report of these DNA repair activities in any member of the Archaea.

journal_name

Microbiol Res

journal_title

Microbiological research

authors

Prail CA,Taylor WD

doi

10.1016/s0944-5013(97)80037-7

subject

Has Abstract

pub_date

1997-09-01 00:00:00

pages

257-60

issue

3

eissn

0944-5013

issn

1618-0623

journal_volume

152

pub_type

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