Abstract：
:Sestrins are highly conserved; stress inducible proteins that help maintain metabolic homeostasis and protect cells under stress conditions. They are up-regulated during stress and influence AMPK and mTOR pathways. Our objective was to find the role of Sestrin protein from Dictyostelium discoideum (Dd), a lower eukaryote where starvation stress initiates multicellular development. The single DdSesn-like gene was expressed and its endogenous functions were characterized. Both, the knockout and constitutively expressing strains were made and their involvement in starvation-induced autophagy was analyzed. Autophagic fluxes and ROS levels were also monitored. Additionally, overexpression of DdSesn decreased cell growth and showed a longer lag phase. Upon starvation both DdSesn and ROS levels increased. SesnOE showed reduced ROS levels while sesn- showed increased ROS levels when compared to the wild type. Therefore, we suggest that increased sesn expression may be beneficial in reducing ROS levels during starvation. Deletion of sesn showed reduced autophagic flux and increased p4EBP1 levels. We show that DdSesn promotes autophagy in D. discoideum upon starvation.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Rafia S,Saran Sdoi
10.1016/j.micres.2018.12.006subject
Has Abstractpub_date
2019-03-01 00:00:00pages
61-71eissn
0944-5013issn
1618-0623pii
S0944-5013(18)30230-1journal_volume
220pub_type
杂志文章abstract：:Leukotoxins produced by staphylococci, especially Staphylococcus aureus, have long been considered important virulence determinants. The present assay examined 26 strains of staphylococci isolated from sheep for their ability to produce factors leukotoxic to polymorphonuclear leukocytes deriving from ovine mammary gla...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(97)80035-3
更新日期：1997-09-01 00:00:00
abstract：:Studies on the microbial ecology of gut microbiota in bats are limited and such information is necessary in determining the ecological significance of these hosts. Short-nosed fruit bats (Cynopterus brachyotis brachyotis) are good candidates for microbiota studies given their close association with humans in urban are...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.04.001
更新日期：2013-10-01 00:00:00
abstract：:Forty-one Lactobacillus strains were tested for antagonistic activity against nine strains of Vibrio. L. plantarum and L. casei were the most effective, and L. brevis was the least effective in inhibiting the growth of Vibrio species. L. gasseri and L. helveticus strains showed higher activity, while L. reuteri and L....
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(98)80011-6
更新日期：1998-11-01 00:00:00
abstract：:The effectiveness of aqueous extracts of various medicinal plants in detoxification of aflatoxin B1 (AFB1) was tested in vitro by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the maximum degradation...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.07.008
更新日期：2014-04-01 00:00:00
abstract：:Araucaria angustifolia, a unique species of this genus that occurs naturally in Brazil, has a high socio-economic and environmental value and is critically endangered of extinction, since it has been submitted to intense predatory exploitation during the last century. Root-associated bacteria from A. angustifolia were...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2011.03.003
更新日期：2012-01-20 00:00:00
abstract：:Sulfate related water quality and trophic status are crucial to operation of water diversion. Though the sulfur geochemistry in the lake sediment have been well studied, the effective indicator of surrounding environment conditions related to sulfur in river-lake systems are still unknown. In this study, Dongping Lake...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126453
更新日期：2020-06-01 00:00:00
abstract：:Polyamines are small polycations essential for cell growth. Ornithine decarboxylase (ODC), the first enzyme of the polyamine synthesis pathway, is one of the most regulated enzymes of eukaryotes. In the present report, the transcriptional regulation of the gene encoding ODC from the dimorphic fungus Yarrowia lipolytic...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.05.001
更新日期：2008-01-01 00:00:00
abstract：:The SAP6 gene (without signal sequence) encoding Metschnikowia reukaufii acid protease was amplified by PCR and fused to the expression vector pET-24a(+). The carboxy-terminal 6x His-tagged recombinant acid protease (rSAP6) was expressed from pET-24a(+)SAP6-6His in Escherichia coli BL21 (DE3) and purified with affinit...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.08.003
更新日期：2010-03-31 00:00:00
abstract：:Alternaria species have been recognized as important cause of allergic diseases and are considered opportunistic pathogens for immunocompromised individuals. In order to assess the contribution of waters to the spread of Alternaria, we examined 390 water samples of various origin for the presence of these microorganis...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(00)80022-1
更新日期：2000-04-01 00:00:00
abstract：:Eight ectomycorrhizal fungal isolates of Pisolithus associated with Eucalyptus species in different parts of India were collected and the genetic variability of these isolates was studied by ITS-RFLP and ITS sequencing. All the isolates showed same RFLP patterns with each restriction enzyme, indicating all these isola...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.03.003
更新日期：2004-01-01 00:00:00
abstract：:Extracellular proteases from 8 Antarctic psychrotolerant Pseudomonas sp. strains were purified and characterised. All of them are neutral metalloproteases, have an apparent molecular mass of 45kDa, optimal activity at 40 degrees C and pH 7-9, retaining significant activity at pH 5-11. With the exception of P96-18, whi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.03.001
更新日期：2004-01-01 00:00:00
abstract：:Bacillus subtilis EA-CB0575 is a plant growth-promoting bacterium (PGPB) associated with banana and tomato crops. Root colonization is an important trait for PGPB microorganisms and potentiates the bacterial effect related to the mechanisms of plant growth promotion. Therefore, detection of bacterial colonization of r...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.08.017
更新日期：2018-12-01 00:00:00
abstract：:The pectinolytic enzyme from the solid-state culture of Rhizopus oryzae NBRC 4707 was purified to homogeneity by column chromatography on CM-Toyopearl 650 M and hydroxylapatite. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 31,000 and was reduced to 29,700 after treat...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.01.001
更新日期：2004-01-01 00:00:00
abstract：:Changes in microbial numbers and activities in a soil in response to bentazon applied at 10 and 100 ppm were studied after 4 and 30 weeks of incubation in laboratory conditions. As regards the eight general and functional microbial groups studied (aerobic and anaerobic bacteria, fungi, aerobic and anaerobic N2-fixing ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(96)80064-4
更新日期：1996-03-01 00:00:00
abstract：:Arbuscular Mycorrhizal Fungi (AMF) live in symbiosis with most crop plants and represent essential elements of soil fertility and plant nutrition and productivity, facilitating soil mineral nutrient uptake and protecting plants from biotic and abiotic stresses. These beneficial services may be mediated by the dense an...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.11.012
更新日期：2016-02-01 00:00:00
abstract：:Salmonella enterica serovar Enteritidis (S. Enteritidis, SE) is a major cause of foodborne diseases for humans. The completeness of the O-chain antigen of Lipopolysaccharide (LPS) determines whether a S. Enteritidis strain is smooth or rough. However, genes that are involved in the synthesis of LPS and rough-smooth va...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.05.001
更新日期：2018-01-01 00:00:00
abstract：:A strain of Fusarium moniliforme isolated from a tropical mangrove ecosystem near Mumbai, India and deposited in the National Collection of Industrial Microorganisms (NCIM) as F. moniliforme NCIM 1276. The organism produced a single extracellular polygalacturonase (PG I) [EC 3.2.1.15] at pH 5 and a single pectate lyas...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.06.002
更新日期：2004-01-01 00:00:00
abstract：:Monodon baculovirls (MBV), external fouling organisms (EFO) and bacteria (especially Vibrio species) were monitored during 1996-1997 at nine different Penaeus monodon rearing hatcheries in India. Total cultivable heterotrophic bacteria, Vibrio-like-bacteria, presumptive Vibrio harveyi, Vibrio anguillarum, Vibrio vulni...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00208
更新日期：2003-01-01 00:00:00
abstract：:The potassium solubilizing microorganisms (KSMs) are a rhizospheric microorganism which solubilizes the insoluble potassium (K) to soluble forms of K for plant growth and yield. K-solubilization is carried out by a large number of saprophytic bacteria (Bacillus mucilaginosus, Bacillus edaphicus, Bacillus circulans, Ac...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2013.09.003
更新日期：2014-05-01 00:00:00
abstract：:Verticillium dahliae is a significant pathogen in cucurbit cropping systems for which there are limited control options outside of soil fumigation. Endophytes, fungi and bacteria that live within plant hosts without impacting the host negatively, have exhibited antagonism to V. dahliae. The objectives of this study we...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126535
更新日期：2020-11-01 00:00:00
abstract：:A microbial consortium reducing high level of perchlorate was developed and in a fed batch bioreactor using acetate as substrate perchlorate was reduced at 0.25 g/g vss. day. Under stable performance, the microbial community structure of the reactor was analyzed through molecular and phenotypic methods. The diversity ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.04.011
更新日期：2015-08-01 00:00:00
abstract：:Probiotics have been used to rebuild the antibiotic-induced dysfunction in gut microbiota, but whether the different strains of probiotics result in similar or reverse effects remains unclear. In this study, the different recovery effects of two cocktails (each contains four strains) of Lactobacillus and fructooligosa...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.04.001
更新日期：2017-07-01 00:00:00
abstract：:Fusaric acid (FA) is a fungal metabolite produced by several Fusarium species responsible for wilts and root rot diseases of a great variety of plants. Bacillus spp. and Pseudomonas spp. have been considered as promising biocontrol agents against phytopathogenic Fusarium spp., however it has been demonstrated that FA ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.09.008
更新日期：2018-01-01 00:00:00
abstract：:Biofilm formation of Group A Streptococcus (GAS) is recognized as an important virulent determinant. The present study reports the antibiofilm potential of seaweed (Gracilaria gracilis) surface associated Bacillus subtilis against GAS. Purification revealed 2,4-Di-tert-butyl-phenol (DTBP) as the active principle. DTBP...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.05.010
更新日期：2016-10-01 00:00:00
abstract：:Saccharomyces cerevisiae and Candida shehatae were co-immobilized in an agar sheet which was introduced in an original two-chambered bioreactor asymmetrically fed in a batch mode with a mixture of glucose and xylose in a ratio of 35:15. The two sugars were consumed simultaneously. All glucose was fermented but only 20...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.07.005
更新日期：2007-01-01 00:00:00
abstract：:Selected isolates of Pseudomonas fluorescens (Pf1-94, Pf4-92, Pf12-94, Pf151-94 and Pf179-94) and chemical resistance inducers (salicylic acid, acetylsalicylic acid, DL-norvaline, indole-3-carbinol and lichenan) were examined for growth promotion and induced systemic resistance against Fusarium wilt of chickpea. A mar...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00202
更新日期：2003-01-01 00:00:00
abstract：:The endophytic bacteria were isolated from coffee roots and seeds in Vietnam and identified with 16S rDNA sequencing as belonging to the Actinobacteria, Firmicutes and Proteobacteria phyla with the Nocardia, Bacillus and Burkholderia as dominant genera, respectively. Out of the thirty genera recovered from Coffea cane...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126613
更新日期：2021-01-01 00:00:00
abstract：:Egyptian soils are generally characterized by slightly alkaline to alkaline pH values (7.5-8.7) which are mainly due to its dry environment. In arid and semi-arid regions, salts are less concentrated and sodium dominates in carbonate and bicarbonate forms, which enhance the formation of alkaline soils. Alkaline soils ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.07.007
更新日期：2014-01-20 00:00:00
abstract：:Application of carbonate precipitation induced by Bacillus pasteurii for improving some properties of cement has been reported. However, it is not yet successful in commercial scale due to the high cost of cultivation medium. This is the first report on the application of effluent from chicken manure bio-gas plant, a ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.03.010
更新日期：2016-05-01 00:00:00
abstract：:The bacterium Helicobacter pylori infects more than 50% of the world population and causes several gastroduodenal diseases, including gastric cancer. Nevertheless, we still need to explore some protein interactions that may be involved in pathogenesis. MreB, an actin homolog, showed some special characteristics in pre...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.04.008
更新日期：2017-08-01 00:00:00
