Abstract:
:Biofilm formation of Group A Streptococcus (GAS) is recognized as an important virulent determinant. The present study reports the antibiofilm potential of seaweed (Gracilaria gracilis) surface associated Bacillus subtilis against GAS. Purification revealed 2,4-Di-tert-butyl-phenol (DTBP) as the active principle. DTBP exhibited a dose dependent antibiofilm activity against GAS (SF370 & six different clinical M serotypes). Microscopic analysis revealed changes in cell surface architecture and reduced thickness upon DTBP treatment. Results of extracellular polymeric substance quantification, microbial adhesion to hydrocarbon assay and fourier transform infrared spectroscopic analysis suggested that DTBP probably interferes with the initial adhesion stage of biofilm formation cascade. Reduction in hyaluronic acid synthesis goes in unison with blood survival assay wherein, increased susceptibility to phagocytosis was observed. In vivo studies using Caenorhabditis elegans manifested the reduction in adherence and virulence, which prompts further investigation of the potential of DTBP for the treatment of GAS infections.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Viszwapriya D,Prithika U,Deebika S,Balamurugan K,Pandian SKdoi
10.1016/j.micres.2016.05.010subject
Has Abstractpub_date
2016-10-01 00:00:00pages
19-31eissn
0944-5013issn
1618-0623pii
S0944-5013(16)30265-8journal_volume
191pub_type
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