Abstract：
:The functions of two long-chain fatty acid CoA ligase genes (facl) in crude oil-degrading Geobacillus thermodenitrificans NG80-2 were characterized. Facl1 and Facl2 encoded by GTNG_0892 and GTNG_1447 were expressed in Escherichia coli and purified as His-tagged fusion proteins. Both enzymes utilized a broad range of fatty acids ranging from acetic acid (C(2)) to melissic acid (C(30)). The most preferred substrates were capric acid (C(10)) for Facl1 and palmitic acid (C(16)) for Facl2, respectively. Both enzymes had an optimal temperature of 60°C, an optimal pH of 7.5, and required ATP as a cofactor. Thermostability of the enzymes and effects of metal ions, EDTA, SDS and Triton X-100 on the enzyme activity were also investigated. When NG80-2 was cultured with crude oil rather than sucrose as the sole carbon source, upregulation of facl1 and facl2 mRNA was observed by real time RT-PCR. This is the first time that the activity of fatty acid CoA ligases toward long-chain fatty acids up to at least C(30) has been demonstrated in bacteria.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Dong Y,Du H,Gao C,Ma T,Feng Ldoi
10.1016/j.micres.2012.05.001subject
Has Abstractpub_date
2012-12-20 00:00:00pages
602-7issue
10eissn
0944-5013issn
1618-0623pii
S0944-5013(12)00059-6journal_volume
167pub_type
杂志文章abstract：:A polygalacturonase with a molecular mass of 74 kDa, an isoelectric point around pH 4.2 and pH--and temperature optima of 3.9 and 50 degrees C, respectively, was purified from a culture fluid of Penicillium frequentans. The enzyme was characterized as an exo-alpha-1,4-polygalacturonase (exo-PG I). Km and Vmax for sodi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00127
更新日期：2002-01-01 00:00:00
abstract：:Increased intestinal absorption of oxalate causes hyperoxaluria, a major risk factor for kidney stone disease. Intestinal colonization of recombinant probiotic bacteria expressing oxalate-degrading gene (OxdC) is an effective therapeutic option for recurrent calcium oxalate (CaOx) stone disease. Therefore, we aimed to...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.06.009
更新日期：2018-10-01 00:00:00
abstract：:Among the 49 strains of moderately halophilic bacteria isolated from the salty environments of Iran, a Gram-positive coccus designated as strain QW6 showed high capacity in the removal of toxic oxyanions of tellurium in a wide range of culture medium factors including pH (5.5-10.5), temperature (25-45 degrees C), vari...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.07.010
更新日期：2008-01-01 00:00:00
abstract：:Long-term continuous cropping has led to epidemic of bacterial wilt disease in Southern China. Bacterial wilt disease is caused by Ralstonia solanacearum and difficult to control. In order to control bacterial wilt, rice hull biochar was applied to soil with different doses (0, 7.5, 15, 30 and 45 t ha-1) in a field tr...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.126373
更新日期：2020-01-01 00:00:00
abstract：:Kineococcus radiotolerans is a Gram-positive, radio-resistant bacterium isolated from a radioactive environment. The small noncoding RNAs (sRNAs) in bacteria are reported to play roles in the immediate response to stress and/or the recovery from stress. The analysis of K. radiotolerans transcriptome sequencing results...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.06.001
更新日期：2016-11-01 00:00:00
abstract：:The saline pond microalga, Dunaliella salina (Dunal) Teod. maintained in De Walne's (basal) medium under laboratory conditions was confirmed by amplifying the chromosomal DNA of the microalga by PCR with specific primers MA1 and MA2. Seaweed extracts obtained from Sargassum wightii and Ulva lactuca were amended separa...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.03.006
更新日期：2007-01-01 00:00:00
abstract：:The operation of wastewater treatment technologies depends on a combination of physical, chemical and biological factors. Microorganisms present in wastewater treatment plants play essential roles in the degradation and removal of organic waste and xenobiotic pollutants. Several microorganisms have been used in comple...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.08.006
更新日期：2016-11-01 00:00:00
abstract：:Application of carbonate precipitation induced by Bacillus pasteurii for improving some properties of cement has been reported. However, it is not yet successful in commercial scale due to the high cost of cultivation medium. This is the first report on the application of effluent from chicken manure bio-gas plant, a ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.03.010
更新日期：2016-05-01 00:00:00
abstract：:Apoptosis stimulated by bacterial toxins is common during infection and is now considered important in disease processes. As a major human pathogen, Staphylococcus aureus also causes apoptosis during infection. In some diseases such as atopic dermatitis and sepsis, the apoptosis induced by S. aureus influences the sev...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2017.08.006
更新日期：2017-12-01 00:00:00
abstract：:Salmonella enterica serovar Enteritidis (S. Enteritidis, SE) is a major cause of foodborne diseases for humans. The completeness of the O-chain antigen of Lipopolysaccharide (LPS) determines whether a S. Enteritidis strain is smooth or rough. However, genes that are involved in the synthesis of LPS and rough-smooth va...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.05.001
更新日期：2018-01-01 00:00:00
abstract：:Although it is known that oxalic acid provides a selective advantage to the secreting microbe our understanding of how this acid is biosynthesized remains incomplete. This study reports the identification, cloning, and partial characterization of the oxalic acid biosynthetic enzyme from the animal bacterial pathogen, ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.11.002
更新日期：2011-10-20 00:00:00
abstract：:Western immunoblot analyses of whole cell lysates probed with a human specific monoclonal anti-topoisomerase IIalpha antibody identified a 190 kDa protein over expressed in the arsenite resistant Leishmania donovani strain. The crude nuclear extract of the resistant strain showed higher topoisomerase II-like enzyme ac...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00177
更新日期：2003-01-01 00:00:00
abstract：:One strain of Lactobacillus acidophilus was found to produce a bacteriocin-like substance in the culture filtrate. The substance was produced in a growth-associated manner, showed heat stability at neutral and acidic pH and exhibited antibacterial activity against various species of Lactobacillus including L. acidophi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00190
更新日期：2003-01-01 00:00:00
abstract：:The potassium solubilizing microorganisms (KSMs) are a rhizospheric microorganism which solubilizes the insoluble potassium (K) to soluble forms of K for plant growth and yield. K-solubilization is carried out by a large number of saprophytic bacteria (Bacillus mucilaginosus, Bacillus edaphicus, Bacillus circulans, Ac...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2013.09.003
更新日期：2014-05-01 00:00:00
abstract：:A microbial consortium reducing high level of perchlorate was developed and in a fed batch bioreactor using acetate as substrate perchlorate was reduced at 0.25 g/g vss. day. Under stable performance, the microbial community structure of the reactor was analyzed through molecular and phenotypic methods. The diversity ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.04.011
更新日期：2015-08-01 00:00:00
abstract：:Actinomycetes are virtually unlimited sources of novel compounds with many therapeutic applications and hold a prominent position due to their diversity and proven ability to produce novel bioactive compounds. There are more than 22,000 known microbial secondary metabolites, 70% of which are produced by actinomycetes,...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2012.06.005
更新日期：2012-12-20 00:00:00
abstract：:Polyamines are small polycations essential for cell growth. Ornithine decarboxylase (ODC), the first enzyme of the polyamine synthesis pathway, is one of the most regulated enzymes of eukaryotes. In the present report, the transcriptional regulation of the gene encoding ODC from the dimorphic fungus Yarrowia lipolytic...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.05.001
更新日期：2008-01-01 00:00:00
abstract：:Extracellular proteases from 8 Antarctic psychrotolerant Pseudomonas sp. strains were purified and characterised. All of them are neutral metalloproteases, have an apparent molecular mass of 45kDa, optimal activity at 40 degrees C and pH 7-9, retaining significant activity at pH 5-11. With the exception of P96-18, whi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.03.001
更新日期：2004-01-01 00:00:00
abstract：:Pseudomonas aeruginosa is one of the most common pathogens associated with nosocomial infections and a great concern to immunocompromised individuals especially in the cases of cystic fibrosis, AIDS and burn wounds. The pathogenicity of P. aeruginosa is largely directed by the quorum sensing (QS) system. Hence, QS may...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.05.001
更新日期：2019-09-01 00:00:00
abstract：:The inhibitory effect of onion oil against the growth of various isolates of bacteria representing Gram-positive (4 isolates) and Gram-negative (4 isolates) species were studied. Results show that onion oil was highly active against all Gram-positive bacteria tested and only one isolate (Klebsiella pneumoniae) of Gram...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(11)80052-2
更新日期：1995-05-01 00:00:00
abstract：:DrrC is a DNA-binding protein of Streptomyces peucetius that provides self-resistance against daunorubicin, the antibiotic produced by the organism. DrrC was expressed in E.coli and purified by using N-terminal MBP-tag which retained DNA-binding property in spite of the tag. Mobility shift assay confirmed the interact...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.05.002
更新日期：2017-09-01 00:00:00
abstract：:Nidus Vespae (honeycomb) is a kind of traditional Chinese medicine that has been demonstrated to inhibit the growth and acid-production of oral cariogenic bacteria. Subsequent studies showed that the chloroform/methanol (Chl/MeOH) chemical extraction of Nidus Vespae was the most effective inhibitor of growth and acido...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2011.03.002
更新日期：2012-01-20 00:00:00
abstract：:The ATP-dependent Lon protease is universally distributed in bacteria, eukaryotic organelles and archaea. In comparison with bacterial and eukaryal Lon proteases, the biology of the archaeal Lon has been studied to a limited extent. In this study, the gene encoding the Lon protease of the alkaliphilic haloarchaeon Nat...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.07.002
更新日期：2011-05-20 00:00:00
abstract：:Alcaligenes sp. strain O-1 inducibly deaminates 2-aminobenzenesulfonate (ABS) via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. This intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway (Scm). The complete pathwa...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.05.006
更新日期：2010-05-30 00:00:00
abstract：:Soybean (Glycine max L.) is among the most economically important legumes that provide more than 1/4 of food (for man) and animal feed. However, its yield is comparatively low, most especially under drought stress. The aim of this study therefore was to assess the ability of Rhizobium spp. and mycorrhizal fungi to enh...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126640
更新日期：2021-01-01 00:00:00
abstract：:Recent studies have shown that the canonical Shine-Dalgarno (SD)-anti-SD interaction is dispensable for the initiation of translation of certain mRNAs in Escherichia coli. Alternative non-SD sequences (located upstream from the initiation codon) and also downstream sequences ("downstream boxes") complementary to 16S r...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(98)80037-2
更新日期：1998-08-01 00:00:00
abstract：:Aneurinibacillus migulanus strains Nagano and NCTC 7096 show potential in biocontrol against fungal and fungus-like plant pathogens, including a wide range of Ascomycota, Basidiomycota, and Oomycetes. Differences in terms of the range of pathogens that each strain inhibits, however, suggested that production of a sing...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.10.007
更新日期：2016-01-01 00:00:00
abstract：:Three surfactin-producing Bacillus subtilis strains, C4, M1 and G2III, previously isolated from honey and intestines from the Apis mellifera L. bee, were phylogenetically characterized at sub-species level as B. subtilis subsp. subtilis using gyrA gene sequencing. The antagonistic effect of surfactin was studied again...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2012.11.004
更新日期：2013-03-30 00:00:00
abstract：:Seventy nine isolates of Botrytis cinerea were collected from different host plants and different locations of India and Nepal. All the isolates were identified as B. cinerea based on morphological features and were confirmed using B. cinerea specific primers. Differentiation among the isolates was assessed using morp...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2014.02.012
更新日期：2014-11-01 00:00:00
abstract：:Biofilms are the most widely distributed and successful microbial modes of life. The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress. Bacteria living in cold environments...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.09.010
更新日期：2019-01-01 00:00:00
