Abstract：
:Pulmonary hemorrhage is an increasing cause of death of leptospirosis patients. Bacterial collagenase has been shown to be involved in lung hemorrhage induced by various infectious agents. According to Leptospira whole genome study, colA, a gene suggested to code for bacterial collagenase has been identified. We investigated colA gene expression in lung tissues of Leptospira infected hamsters. Golden Syrian Hamsters were injected intraperitoneally with Leptospira interrogans serovar Pyrogenes. The hamsters were sacrificed on days 3, 5 and 7 post-infection and lung tissues were collected for histological examination and RNA extraction. Lung pathologies including atelectasis and hemorrhage were observed. Expression of colA gene in lung tissues was demonstrated by both RT-PCR and real time PCR. In addition, ColA protein was cloned and the purified protein could react with sera from leptospirosis patients. Leptospira ColA protein may play a role in Leptospira survival or pathogenesis in vivo. Its reaction with leptospirosis sera suggests that this protein is immunogenic and could be another candidate for vaccine development.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Janwitthayanan W,Keelawat S,Payungporn S,Lowanitchapat A,Suwancharoen D,Poovorawan Y,Chirathaworn Cdoi
10.1016/j.micres.2012.12.005subject
Has Abstractpub_date
2013-06-12 00:00:00pages
268-72issue
5eissn
0944-5013issn
1618-0623pii
S0944-5013(12)00144-9journal_volume
168pub_type
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