Subtypes of genotype A Candida albicans isolates determined by restriction endonuclease and sequence analyses.

Abstract:

:Systemic yeast infections are the Leading cause of mortality and morbidity in immunocompromized patients. Candida albicans, being the most frequently isolated fungal pathogen in these patients, can be divided into three genotypes (genotypes A, B and C) by 25S intron analysis. In our study, we found that molecular sizes of genotype A C. albicans isolates were heterogeneous. In order to determine the molecular basis of this difference, Haelll digestion was applied, and strains forming different band patterns were analyzed by automated sequence analysis. As a result of sequence analysis, eight different subtypes (a --> h) were found among genotype A C. albicans strains and an easy differentiation scheme consisting of Haelll and MspI digestions was constructed.

journal_name

Microbiol Res

journal_title

Microbiological research

authors

Karahan ZC,Akar N

doi

10.1016/j.micres.2005.02.010

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

361-6

issue

4

eissn

0944-5013

issn

1618-0623

pii

S0944-5013(05)00028-5

journal_volume

160

pub_type

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