Abstract：
:Collagen-like proteins (CLPs) share the distinctive Gly-X-Thr repeating amino acid sequence of animal collagens, and contain N- and C-terminal domain making a collagen-like structure in Bacillus amyloliquefaciens FZB42, a plant growth-promoting rhizobacterium. Our previous study demonstrated that CLPs play important roles in biofilm construction and adherence to the surfaces on plant roots. However, bacterial localization of the CLPs remains unclear. Here, disrupted strains on all four clp genes (clpA, clpB, clpC and clpD) shown fewer filament than wild-type bacteria in extracellular matrix under scanning electron microscope (SEM). Transmission electron microscopy (TEM) was used to observe the differences on filament which associated on the cell surface, then the CLPs mutation strains showed less flagella than the wild type. Immunogold labeling determined the location that ClpB and ClpC localized on the flagella surface. In addition, western blotting analysis of crude flagella extracts suggested that the ClpB and ClpC are associated to flagella as well. The mutation strains also reduced motility of swimming on the surface of soft agar medium and changed the architectural of microcolony biofilm edge. The study suggests that collagen-like protein ClpB and ClpC, as novel proteins, associated with flagella in B. amyloliquefaciens.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Zhao X,Wang R,Shang Q,Hao H,Li Y,Zhang Y,Guo Z,Wang Y,Xie Zdoi
10.1016/j.micres.2015.12.004subject
Has Abstractpub_date
2016-03-01 00:00:00pages
25-31eissn
0944-5013issn
1618-0623pii
S0944-5013(15)30039-2journal_volume
184pub_type
杂志文章abstract：:Twenty-nine rhizobial isolates from root nodules of the wild Legumes Astragalus, Lespedeza and Hedysarum growing in the northwestern region of China, were characterized by numerical taxonomy, RFLP and sequencing of PCR-amplified 16S rDNA genes, and cross-nodulation with selected Legume species. Based on the results fr...
journal_title：Microbiological research
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pub_type： 杂志文章
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journal_title：Microbiological research
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更新日期：2018-11-01 00:00:00
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journal_title：Microbiological research
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pub_type： 杂志文章
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更新日期：2016-09-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
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更新日期：2010-02-28 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.05.001
更新日期：2008-01-01 00:00:00
