Abstract：
:The genes that encode the alpha and beta subunits of protocatechuate 3,4-dioxygenase (3,4-PCD [EC 1.13.11.3]) were cloned from a Pseudomonas marginata genomic library. These genes pcaG and pcaH, were found when screening the library for hydrolase genes. The two open reading frames of the PCD genes could be identified adjacent to an esterase gene by sequence homology. A 1.7-kb KpnI/ApaI fragment, carrying pcaG and pcaH, was subcloned and the genes were functionally expressed in Escherichia coli. The deduced amino acid sequence shows high homology to previously determined amino acid sequences of bacterial protocatechuate 3,4-dioxygenases. A homology model based on the available crystal structure of the protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa shows high similarity with the binding and catalytic sites.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Petersen E,Zuegg J,Ribbons DW,Schwab Hdoi
10.1016/s0944-5013(96)80004-8subject
Has Abstractpub_date
1996-12-01 00:00:00pages
359-70issue
4eissn
0944-5013issn
1618-0623journal_volume
151pub_type
杂志文章abstract：:The BIOLOG system was used to generate knowledge of enterococci populations found in fresh and dry manure of livestock (cattle (Bos taurus), horse (Equus caballus), and sheep (Ovis aires)). Six-hundred and forty Enterococcus isolates from the host sources were observed as a combined fresh manure unit and a combined dr...
journal_title：Microbiological research
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journal_title：Microbiological research
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journal_title：Microbiological research
pub_type： 杂志文章,评审
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journal_title：Microbiological research
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journal_title：Microbiological research
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更新日期：2017-01-01 00:00:00
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