Abstract：
:Since their discovery, plant-growth promoting rhizobacteria from the genus Azospirillum have been subjected to intensive research due to their biotechnological potential as crop inoculants. Phylogenetic analysis of Azospirillum spp. is carried out by 16S rRNA sequencing almost exclusively, but inconsistencies and low confidence often arise when working with close species. In this work, it was observed that these difficulties might be explained by a high number of rRNA operons with considerable inter-genic variability within Azospirillum genomes. To search for alternative genetic markers from a list of housekeeping genes, the correlation between pairwise gene and whole-genome similarities was examined. Due to its good performance, rpoD was selected for further analyses. Genus-specific primers for the PCR-amplification and sequencing of rpoD from Azospirillum spp. were designed and tested on 16 type strains of different species. The sequences obtained were used for inferring a phylogenetic tree of the genus, which was in turn used as a reference to successfully identify a collection of 31 azospirilla isolated from many different locations of Argentine. In addition, several strains that might represent novel species were detected. The results indicate that the sequencing of rpoD is a suitable alternative method for a confident molecular identification in Azospirillum spp.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Maroniche GA,García JE,Salcedo F,Creus CMdoi
10.1016/j.micres.2016.11.009subject
Has Abstractpub_date
2017-01-01 00:00:00pages
1-10eissn
0944-5013issn
1618-0623pii
S0944-5013(16)30732-7journal_volume
195pub_type
杂志文章abstract：:Diseases in plants are mostly caused by fungi. Fungal interactions with the host can be either biotrophic, necrotrophic or hemibiotrophic. Synergistic polymicrobial interactions have been recently recognized that can also attribute to the occurrence of complex plant diseases. Tea is one of the most widely consumed bev...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126561
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journal_title：Microbiological research
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doi：10.1016/j.micres.2005.12.001
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journal_title：Microbiological research
pub_type： 杂志文章,评审
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journal_title：Microbiological research
pub_type： 杂志文章
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.03.004
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abstract：:In Salmonella Typhimurium (S. Typhimurium), lipopolysaccharide (LPS) anchored on the bacterial outer membrane is a major immune stimulus that can broadly activate immune cells and induce innate immune responses. wzxE is involved in bacterial LPS biosynthesis but has rarely been reported in Salmonella; wzxE encodes a f...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126502
更新日期：2020-09-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.01.003
更新日期：2009-01-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.04.011
更新日期：2013-11-07 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(98)80003-7
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abstract：:Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease (JD) in animals, has also been linked with Crohn's disease in human beings. Lack of indigenous diagnostics and vaccine hampered control of JD in India. Designing effective control strategies require thorough understanding of ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.03.007
更新日期：2010-02-28 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.01.002
更新日期：2010-02-28 00:00:00
abstract：:The SAP6 gene (without signal sequence) encoding Metschnikowia reukaufii acid protease was amplified by PCR and fused to the expression vector pET-24a(+). The carboxy-terminal 6x His-tagged recombinant acid protease (rSAP6) was expressed from pET-24a(+)SAP6-6His in Escherichia coli BL21 (DE3) and purified with affinit...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2008.08.003
更新日期：2010-03-31 00:00:00
abstract：:Pulmonary hemorrhage is an increasing cause of death of leptospirosis patients. Bacterial collagenase has been shown to be involved in lung hemorrhage induced by various infectious agents. According to Leptospira whole genome study, colA, a gene suggested to code for bacterial collagenase has been identified. We inves...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2012.12.005
更新日期：2013-06-12 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.07.012
更新日期：2017-01-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.06.012
更新日期：2007-01-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
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更新日期：2015-07-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126525
更新日期：2020-09-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
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更新日期：2013-11-07 00:00:00
abstract：:Hyicin 3682, the first bacteriocin reported for Staphylococcus hyicus, is a BsaCOL variant produced by S. hyicus 3682, a strain isolated from bovine milk. Hyicin 3682 is found in the culture supernatant, is bactericidal and its producing strain exhibits a much broader spectrum of antimicrobial activity than the produc...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.02.003
更新日期：2017-05-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(97)80012-2
更新日期：1997-07-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
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更新日期：2020-06-01 00:00:00
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journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.03.010
更新日期：2016-05-01 00:00:00
abstract：:Hydrogenases are the key enzymes for the biological hydrogen production, which can be classified as H(2)-uptake hydrogenase and H(2)-production hydrogenase. The genes encoding a membrane-bound [NiFe]-hydrogenase (MBH), which is mainly responsible for hydrogen uptake, from the photosynthetic bacterium Allochromatium vi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.03.001
更新日期：2009-01-01 00:00:00
abstract：:Bacterial carbonate precipitation has implications in geological processes and important biotechnological applications. Bacteria capable of precipitating carbonates have been isolated from different calcium carbonate deposits (speleothems) in caves, soil, freshwater and seawater around the world. However, the diversit...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.09.006
更新日期：2016-01-01 00:00:00
abstract：:A strain of Fusarium moniliforme isolated from a tropical mangrove ecosystem near Mumbai, India and deposited in the National Collection of Industrial Microorganisms (NCIM) as F. moniliforme NCIM 1276. The organism produced a single extracellular polygalacturonase (PG I) [EC 3.2.1.15] at pH 5 and a single pectate lyas...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.06.002
更新日期：2004-01-01 00:00:00
abstract：:A mercury-resistant plasmid of pTMJ212 which was able to shuttle between Acidithiobacillus caldus and Escherichia coli was constructed by inserting the mercury resistant determinants, the mer operon of Acidithiobacillus ferrooxidans, into the IncQ plasmid of pJRD215. pTMJ212 was transferred from Escherichia coli into ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.10.003
更新日期：2011-10-20 00:00:00
abstract：:β-lactamase inhibitors are potent synergistic drugs to deteriorate the multidrug-resistant bacteria. Here, we report the β-lactamase inhibitory ability of kalafungin isolated from a marine sponge derived Streptomyces sp. SBRK1. The IC50 value of the kalafungin was calculated as 225.37 ± 1.95 μM against β-lactamase. Th...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126666
更新日期：2021-03-01 00:00:00
abstract：:Long-term continuous cropping has led to epidemic of bacterial wilt disease in Southern China. Bacterial wilt disease is caused by Ralstonia solanacearum and difficult to control. In order to control bacterial wilt, rice hull biochar was applied to soil with different doses (0, 7.5, 15, 30 and 45 t ha-1) in a field tr...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.126373
更新日期：2020-01-01 00:00:00
abstract：:Leukotoxins produced by staphylococci, especially Staphylococcus aureus, have long been considered important virulence determinants. The present assay examined 26 strains of staphylococci isolated from sheep for their ability to produce factors leukotoxic to polymorphonuclear leukocytes deriving from ovine mammary gla...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(97)80035-3
更新日期：1997-09-01 00:00:00
