当前位置： SCI文献检索 > MICROBIOLOGICAL RESEARCH期刊下所有文献 > Δ9 desaturase from Trypanosoma cruzi: Key enzyme in the parasite metabolism. Cloning and overexpression.

Δ9 desaturase from Trypanosoma cruzi: Key enzyme in the parasite metabolism. Cloning and overexpression.

Abstract：

:Desaturases, key enzymes in the metabolism of fatty acids, regulate the physical and biochemical properties of membranes. They adjust the composition of saturated and unsaturated fatty acids in response to changes in the environmental. We demonstrated the existence of Δ9 desaturase activity in epimastigotes of the Trypanosoma cruzi Tulahuen strain. In the present study, showed that this enzyme has an approximate molecular mass of 50kDa and a pI value of approximately 9. In order to characterize the Δ9 desaturase of Trypanosoma cruzi, (TcΔ9DES) we have cloned, sequenced and expressed in Escherichia coli. The gene consists of 1300bp and encodes a peptide of 433 amino acids with a molecular weight of 50kDa. Analysis of the amino acid sequence revealed three clusters of histidine and two hydrophobic regions, characteristic of membrane-bound desaturases. Gene expression studies showed that TcΔ9DES was overexpressed as an active protein. Fatty acid analysis showed that the expressed protein was confirmed to be functional with Δ9 desaturase activity. This enzyme changed the fatty acid profile of TcΔ9DES-expressing E. coli, decreasing the levels of palmitic (16:0) and stearic (18:0) acids and enhancing palmitoleic (16:1Δ9) and monounsaturated 18 carbons fatty acids. When [1-14C]palmitic or [1-14C]stearic acid was used as substrate, TcΔ9DES-expressing E. coli exhibited high desaturase activity associated with increased levels of monounsaturated fatty acids, suggesting that the TcΔ9DES enzyme was actively expressed in E. coli. To check the commitment of TcΔ9DES against sterol biosynthesis inhibitors we tested the activity under ketoconazole effect. Native TcΔ9DES, showed a significant activity inhibition. Since TcΔ9DES has shown active participation under different environmental factors, among them, ketoconazole, we consider that it plays a critical role in the metabolism of the parasite.

journal_name

Microbiol Res

journal_title

Microbiological research

authors

Woelke MR,Paulucci NS,Selva A,Garban H,de Lema MG

doi

10.1016/j.micres.2016.07.012

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

29-37

eissn

0944-5013

issn

1618-0623

pii

S0944-5013(16)30517-1

journal_volume

194

pub_type

杂志文章

相关文献

MICROBIOLOGICAL RESEARCH文献大全
  • Purification of the extracellular pectinolytic enzyme from the fungus Rhizopus oryzae NBRC 4707.

    abstract：:The pectinolytic enzyme from the solid-state culture of Rhizopus oryzae NBRC 4707 was purified to homogeneity by column chromatography on CM-Toyopearl 650 M and hydroxylapatite. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 31,000 and was reduced to 29,700 after treat...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2004.01.001

    authors： Saito K,Takakuwa N,Oda Y

    更新日期：2004-01-01 00:00:00

  • Annual ryegrass-associated bacteria with potential for plant growth promotion.

    abstract：:Annual ryegrass is a fast-growing cool-season grass broadly present in the Portuguese "montado", a typically Mediterranean agro-forestry-pastoral ecosystem. A culture-dependent approach was used to investigate natural associations of this crop with potentially beneficial bacteria, aiming to identify strains suitable f...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2013.12.010

    authors： Castanheira N,Dourado AC,Alves PI,Cortés-Pallero AM,Delgado-Rodríguez AI,Prazeres Â,Borges N,Sánchez C,Barreto Crespo MT,Fareleira P

    更新日期：2014-09-01 00:00:00

  • Influence of salinity on methanogenesis and associated microflora in tropical rice soils.

    abstract：:In a laboratory incubation study, methane (CH4) production in two saline soils and a nonsaline soil sample was investigated under flooded conditions. Mean CH4 production was remarkable (630.86 ng CH4/g) in nonsaline alluvial soil, but low (12.97 ng CH4/g) in acid sulfate saline (Pokkali) soil which was attributed to t...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/S0944-5013(00)80035-X

    authors： Pattnaik P,Mishra SR,Bharati K,Mohanty SR,Sethunathan N,Adhya TK

    更新日期：2000-09-01 00:00:00

  • Physiological role of the novel salicylaldehyde dehydrogenase NahV in mineralization of naphthalene by Pseudomonas putida ND6.

    abstract：:The classical salicylaldehyde dehydrogenases found in naphthalene-degrading bacteria are denoted as NahF. In addition to NahF, NahV, and its corresponding gene nahV, were found here in multiple naphthalene-degrading bacteria isolated from industrial wastewater polluted with polycyclic aromatic hydrocarbons (PAHs). In ...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2011.01.003

    authors： Li S,Li X,Zhao H,Cai B

    更新日期：2011-12-20 00:00:00

  • Responses of Halobacterium halobium and Sulfolobus solfataricus to hydrogen peroxide and N-methyl-N'-nitro-N-nitrosoguanidine [correction of N-methyl-N-nitrosoguanidine] exposure.

    abstract：:We have examined the capacity of Halobacterium halobium and Sulfolobus solfataricus to withstand the lethal effect of hydrogen peroxide and N-methyl-N'-nitro-N-nitrosoguanidine [corrected]. We tested a variety of pretreatment regimens with both mutagens and all failed to elicit an inducible response to the lethal eff...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/s0944-5013(97)80037-7

    authors： Prail CA,Taylor WD

    更新日期：1997-09-01 00:00:00

  • Phenotypic and genotypic evaluation of slime production by conventional and molecular microbiological techniques.

    abstract：:Twenty-nine staphylococcal isolates from different clinical samples were tested for slime production: phenotypic characterization was carried out using Christensen test (tube test) and Congo red agar plate test (CRA plate test), while the presence and expression of icaA and icaD genes were evaluated by real-time PCR. ...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2007.04.004

    authors： Liberto MC,Matera G,Quirino A,Lamberti AG,Capicotto R,Puccio R,Barreca GS,Focà E,Cascio A,Focà A

    更新日期：2009-01-01 00:00:00

  • Effects of cyclophosphamide on immune system and gut microbiota in mice.

    abstract：:Cyclophosphamide (CP) is the most commonly used drug in autoimmune disease, cancer, blood and marrow transplantation. Recent data revealed that therapy efficacy of CP is gut microbiota-dependent. So, it is very important to understand how CP affects intestinal microbiota and immune function. In this study, the effects...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2014.11.002

    authors： Xu X,Zhang X

    更新日期：2015-02-01 00:00:00

  • Identification of binding sites of Lactobacillus plantarum enolase involved in the interaction with human plasminogen.

    abstract：:The enolase EnoA1 of Lactobacillus plantarum is here shown to interact with human plasminogen (Plg). By sequence alignment of EnoA1 with Streptococcus pneumoniae and Bifidobacterium lactis enolases, we identified BS1 and BS2 Plg-binding sites. A structure prediction of EnoA1 showed lysine residues in position 255 (BS2...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2012.10.001

    authors： Vastano V,Capri U,Candela M,Siciliano RA,Russo L,Renda M,Sacco M

    更新日期：2013-02-22 00:00:00

  • Evaluation of selenite bioremoval from liquid culture by Enterococcus species.

    abstract：:The genus Enterococcus belong to the genera of bacteria that produce lactic acid and can confer health benefits to living organisms. Selenium (Se) is an essential micronutrient for humans and animals. Thirty-six Enterococcus species isolated from dairy products were screened for Se(IV) sorption capacity for use as a p...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2010.03.005

    authors： Pieniz S,Okeke BC,Andreazza R,Brandelli A

    更新日期：2011-03-20 00:00:00

  • MarTrack: A versatile toolbox of mariner transposon derivatives used for functional genetic analysis of bacterial genomes.

    abstract：:The mariner transposon family of Himar1 has been widely used for the random mutagenesis of bacteria to generate single insertions into the chromosome. Here, a versatile toolbox of mariner transposon derivatives was generated and applied to the functional genomics investigation of fish pathogen Edwardsiella piscicida. ...

    journal_title：Microbiological research

    pub_type： 杂志文章,收录出版

    doi：10.1016/j.micres.2018.11.008

    authors： Wei L,Qiao H,Liu B,Yin K,Liu Q,Zhang Y,Ma Y,Wang Q

    更新日期：2019-02-01 00:00:00

  • The exoproteome profiles of three Staphylococcus saprophyticus strains reveal diversity in protein secretion contents.

    abstract：:Staphylococcus saprophyticus is a gram-positive microorganism responsible for urinary tract infections (UTIs). Although some virulence factors are characterized, such as urease, autolysins, adhesins and hemagglutinins, large-scale proteomic studies have not been performed within this species. We performed the characte...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2018.08.008

    authors： Oliveira AS,Rosa IIR,Novaes E,Oliveira LS,Baeza LC,Borges CL,Marlinghaus L,Soares CMA,Giambiagi-deMarval M,Parente-Rocha JA

    更新日期：2018-11-01 00:00:00

  • Mating-type switching and mating-type gene array expression in the methylotrophic yeast Ogataea thermomethanolica TBRC656.

    abstract：:The methylotrophic yeast, Ogataea thermomethanolica TBRC656, is an attractive host organism for heterologous protein production owing to the availability of protein expression vectors and a genome-editing tool. In this study, we focused on mating-type switching and gene expression in order to elucidate its sexual life...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2019.126372

    authors： Wongwisansri S,Promdonkoy P,Likhitrattanapisal S,Harnpichanchai P,Fujiyama K,Kaneko Y,Eurwilaichitr L,Ingsriswang S,Tanapongpipat S

    更新日期：2020-02-01 00:00:00

  • Isolation, selection and characterization of root-associated growth promoting bacteria in Brazil Pine (Araucaria angustifolia).

    abstract：:Araucaria angustifolia, a unique species of this genus that occurs naturally in Brazil, has a high socio-economic and environmental value and is critically endangered of extinction, since it has been submitted to intense predatory exploitation during the last century. Root-associated bacteria from A. angustifolia were...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2011.03.003

    authors： Ribeiro CM,Cardoso EJ

    更新日期：2012-01-20 00:00:00

  • Multifunctionality and diversity of culturable bacterial communities strictly associated with spores of the plant beneficial symbiont Rhizophagus intraradices.

    abstract：:Arbuscular Mycorrhizal Fungi (AMF) live in symbiosis with most crop plants and represent essential elements of soil fertility and plant nutrition and productivity, facilitating soil mineral nutrient uptake and protecting plants from biotic and abiotic stresses. These beneficial services may be mediated by the dense an...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2015.11.012

    authors： Battini F,Cristani C,Giovannetti M,Agnolucci M

    更新日期：2016-02-01 00:00:00

  • Molecular identification of Azospirillum spp.: Limitations of 16S rRNA and qualities of rpoD as genetic markers.

    abstract：:Since their discovery, plant-growth promoting rhizobacteria from the genus Azospirillum have been subjected to intensive research due to their biotechnological potential as crop inoculants. Phylogenetic analysis of Azospirillum spp. is carried out by 16S rRNA sequencing almost exclusively, but inconsistencies and low ...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2016.11.009

    authors： Maroniche GA,García JE,Salcedo F,Creus CM

    更新日期：2017-01-01 00:00:00

  • Antibacterial activity of Lactobacillus species against Vibrio species.

    abstract：:Forty-one Lactobacillus strains were tested for antagonistic activity against nine strains of Vibrio. L. plantarum and L. casei were the most effective, and L. brevis was the least effective in inhibiting the growth of Vibrio species. L. gasseri and L. helveticus strains showed higher activity, while L. reuteri and L....

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/s0944-5013(98)80011-6

    authors： Koga T,Mizobe T,Takumi K

    更新日期：1998-11-01 00:00:00

  • Current situation of biofuel production and its enhancement by CRISPR/Cas9-mediated genome engineering of microbial cells.

    abstract：:Geopolitical and economic factors have motivated the scientific community to utilize renewable energy resources. In addition to the modifications in major steps and processes of biofuel production, manipulation of microbial genome engineering tools is essential in order to find sustainable solution of continuous deple...

    journal_title：Microbiological research

    pub_type： 杂志文章,评审

    doi：10.1016/j.micres.2018.10.010

    authors： Javed MR,Noman M,Shahid M,Ahmed T,Khurshid M,Rashid MH,Ismail M,Sadaf M,Khan F

    更新日期：2019-02-01 00:00:00

  • Composition and functional comparison of vetiver root endophytic microbiota originating from different geographic locations that show antagonistic activity towards Fusarium graminearum.

    abstract：:Given the current trend towards reducing the use of chemical controls in agriculture, microbial resources such as plant endophytes are being intensively investigated for traits that are conducive to plant protection. Among the various important target pathogens, Fusarium graminearum is a fungal pathogen of cereal crop...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2020.126650

    authors： Munakata Y,Gavira C,Genestier J,Bourgaud F,Hehn A,Slezack-Deschaumes S

    更新日期：2021-02-01 00:00:00

  • Abundance of potentially pathogenic micro-organisms in Penaeus monodon larvae rearing systems in India.

    abstract：:Monodon baculovirls (MBV), external fouling organisms (EFO) and bacteria (especially Vibrio species) were monitored during 1996-1997 at nine different Penaeus monodon rearing hatcheries in India. Total cultivable heterotrophic bacteria, Vibrio-like-bacteria, presumptive Vibrio harveyi, Vibrio anguillarum, Vibrio vulni...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1078/0944-5013-00208

    authors： Vaseeharan B,Ramasamy P

    更新日期：2003-01-01 00:00:00

  • Mitochondria thioredoxin's backup role in oxidative stress resistance in Trichoderma reesei.

    abstract：:Microorganisms often suffer from oxidative stress created from nutrient starvation and environmental changes. Thioredoxin (Trx) and glutathione (GSH) pathways are believed critical in related protective functions. The roles of Trx in improving abiotic stress resistance in Trichoderma reesei are still unclear. In this ...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2015.01.005

    authors： Wang G,Wang H,Xiong X,Chen S,Zhang D

    更新日期：2015-02-01 00:00:00

  • Forest tree associated bacteria for potential biological control of Fusarium solani and of Fusarium kuroshium, causal agent of Fusarium dieback.

    abstract：:Although the use of crop-associated bacteria as biological control agents of fungal diseases has gained increasing interest, the biotechnological potential of forest tree-associated microbes and their natural products has scarcely been investigated. The objective of this study was to identify bacteria or bacterial pro...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2020.126440

    authors： Báez-Vallejo N,Camarena-Pozos DA,Monribot-Villanueva JL,Ramírez-Vázquez M,Carrión-Villarnovo GL,Guerrero-Analco JA,Partida-Martínez LP,Reverchon F

    更新日期：2020-05-01 00:00:00

  • Characterization of bacterial diversity in two aerated lagoons of a wastewater treatment plant using PCR-DGGE analysis.

    abstract：:Aerated lagoons are commonly used for domestic and industrial wastewater treatment due to their low cost and minimal need of operational requirements. However, little information is known regarding microbial communities that inhabit these ecosystems. In this study, a 16S-DGGE approach was used to estimate bacterial di...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2007.06.005

    authors： Moura A,Tacão M,Henriques I,Dias J,Ferreira P,Correia A

    更新日期：2009-01-01 00:00:00

  • Identification of putative effector genes and their transcripts in three strains related to 'Candidatus Phytoplasma aurantifolia'.

    abstract：:Molecular mechanisms underlying phytoplasma interactions with host plants are largely unknown. In this study attempts were made to identify effectors of three phytoplasma strains related to 'Ca. P. aurantifolia', crotalaria phyllody (CrP), faba bean phyllody (FBP), and witches' broom disease of lime (WBDL), using info...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2017.03.001

    authors： Anabestani A,Izadpanah K,Abbà S,Galetto L,Ghorbani A,Palmano S,Siampour M,Veratti F,Marzachì C

    更新日期：2017-06-01 00:00:00

  • Environmental conditions shape the biofilm of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.

    abstract：:Biofilms are the most widely distributed and successful microbial modes of life. The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress. Bacteria living in cold environments...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2018.09.010

    authors： Ricciardelli A,Casillo A,Vergara A,Balasco N,Corsaro MM,Tutino ML,Parrilli E

    更新日期：2019-01-01 00:00:00

  • Active substances against trypomastigote forms of Trypanosoma cruzi and microorganisms are produced in sequence by Talaromyces flavus.

    abstract：:The conditions for the sequential production of antibiotic activity by Talaromyces flavus were determined. The highest level of activity against Trypanosoma cruzi was obtained from the aqueous extract of the Czapeck's fermentative culture after 48 hours, with lysis of 97.58% of the trypomastigote forms of Trypanosoma ...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1078/0944-5013-00148

    authors： Freitas TP,Furtado NA,Bastos JK,Said S

    更新日期：2002-01-01 00:00:00

  • The new flagella-associated collagen-like proteins ClpB and ClpC of Bacillus amyloliquefaciens FZB42 are involved in bacterial motility.

    abstract：:Collagen-like proteins (CLPs) share the distinctive Gly-X-Thr repeating amino acid sequence of animal collagens, and contain N- and C-terminal domain making a collagen-like structure in Bacillus amyloliquefaciens FZB42, a plant growth-promoting rhizobacterium. Our previous study demonstrated that CLPs play important r...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2015.12.004

    authors： Zhao X,Wang R,Shang Q,Hao H,Li Y,Zhang Y,Guo Z,Wang Y,Xie Z

    更新日期：2016-03-01 00:00:00

  • Diversity in soil fungi from undisturbed and disturbed Celtis tala and Scutia buxifolia forests in the eastern Buenos Aires province (Argentina).

    abstract：:The rhizospheric soil microfungi from a native forest (undisturbed and disturbed) were studied using soil dilution plate and soil washing methods. Fungi were isolated using slightly acid and alkaline culture media. 54 taxa were isolated: 49 from undisturbed forest soil and 37 from disturbed forest soil. Acremonium sp....

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1078/0944-5013-00138

    authors： Cabello M,Arambarri A

    更新日期：2002-01-01 00:00:00

  • Role of salicylic acid in systemic resistance induced by Pseudomonas fluorescens against Fusarium oxysporum f. sp. ciceri in chickpea.

    abstract：:Selected isolates of Pseudomonas fluorescens (Pf1-94, Pf4-92, Pf12-94, Pf151-94 and Pf179-94) and chemical resistance inducers (salicylic acid, acetylsalicylic acid, DL-norvaline, indole-3-carbinol and lichenan) were examined for growth promotion and induced systemic resistance against Fusarium wilt of chickpea. A mar...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1078/0944-5013-00202

    authors： Saikia R,Singh T,Kumar R,Srivastava J,Srivastava AK,Singh K,Arora DK

    更新日期：2003-01-01 00:00:00

  • Purification and partial characterization of exopolygalacturonase I from Penicillium frequentans.

    abstract：:A polygalacturonase with a molecular mass of 74 kDa, an isoelectric point around pH 4.2 and pH--and temperature optima of 3.9 and 50 degrees C, respectively, was purified from a culture fluid of Penicillium frequentans. The enzyme was characterized as an exo-alpha-1,4-polygalacturonase (exo-PG I). Km and Vmax for sodi...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1078/0944-5013-00127

    authors： dos Santos Cunha Chellegatti MA,Fonseca MJ,Said S

    更新日期：2002-01-01 00:00:00

  • Subtypes of genotype A Candida albicans isolates determined by restriction endonuclease and sequence analyses.

    abstract：:Systemic yeast infections are the Leading cause of mortality and morbidity in immunocompromized patients. Candida albicans, being the most frequently isolated fungal pathogen in these patients, can be divided into three genotypes (genotypes A, B and C) by 25S intron analysis. In our study, we found that molecular size...

    journal_title：Microbiological research

    pub_type： 杂志文章

    doi：10.1016/j.micres.2005.02.010

    authors： Karahan ZC,Akar N

    更新日期：2005-01-01 00:00:00