Abstract：
:In search of the optimal way to reduce the hazards of environmental contamination by formaldehyde (FD) and methanol the use of unconventional yeasts is proposed as exemplified by the methylotrophic yeast Hansenula polymorpha. In a very simplified environment of a model wastewater solution, H. polymorpha cells were able to grow on, and metabolize formaldehyde and methanol, applied as sole carbon sources, at concentrations typical for wastewaters of the chemical industry. Several experimental conditions were tested for cell growth and biodegradation kinetics. It was found that the yeast culture inoculated at low cell density was able to grow on initial FD levels up to 400mg/l and the biomass yield was dependent on both, the amount of total carbon added and the physiological state of the cells. When high density of pre-adapted cell culture was used, the methylotrophs were fully viable and able to degrade formaldehyde present at initial concentrations up to 700 mg/l. The maximum limiting FD consumption rate was determined as approx. 400 mg/1 per hour. Methanol, at concentrations up to 2%, was easily utilized and did not have a negative effect on cell growth and respiration. It is suggested that in real wastewaters the eukaryotic microorganisms--in contrast to bacteria--might reveal greater adaptation potential to toxic levels of formaldehyde as well as to other wastewater constituents.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Kaszycki P,Kołoczek Hdoi
10.1016/S0944-5013(00)80002-6subject
Has Abstractpub_date
2000-03-01 00:00:00pages
289-96issue
4eissn
0944-5013issn
1618-0623pii
S0944-5013(00)80002-6journal_volume
154pub_type
杂志文章abstract：:Arbuscular Mycorrhizal Fungi (AMF) live in symbiosis with most crop plants and represent essential elements of soil fertility and plant nutrition and productivity, facilitating soil mineral nutrient uptake and protecting plants from biotic and abiotic stresses. These beneficial services may be mediated by the dense an...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.11.012
更新日期：2016-02-01 00:00:00
abstract：:In a laboratory incubation study, methane (CH4) production in two saline soils and a nonsaline soil sample was investigated under flooded conditions. Mean CH4 production was remarkable (630.86 ng CH4/g) in nonsaline alluvial soil, but low (12.97 ng CH4/g) in acid sulfate saline (Pokkali) soil which was attributed to t...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(00)80035-X
更新日期：2000-09-01 00:00:00
abstract：:Moderately halophilic bacteria are a kind of extreme environment microorganism that can tolerate moderate salt concentrations ranging from 0.5M to 2.5M. Here, via a metagenomic library screen, we identified four putative Na(+) transporters, designated H7-Nha, H16-Mppe, H19-Cap and H35-Mrp, from moderately halophilic c...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2012.06.004
更新日期：2013-02-22 00:00:00
abstract：:Tannase producing fungal strains were isolated from different locations including garbages, forests and orchards, etc. The strain giving maximum enzyme yield was identified to be Aspergillus ruber. Enzyme production was studied under solid state fermentation using different tannin rich substrates like ber leaves (Zyzy...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.06.012
更新日期：2007-01-01 00:00:00
abstract：:Cyclophosphamide (CP) is the most commonly used drug in autoimmune disease, cancer, blood and marrow transplantation. Recent data revealed that therapy efficacy of CP is gut microbiota-dependent. So, it is very important to understand how CP affects intestinal microbiota and immune function. In this study, the effects...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2014.11.002
更新日期：2015-02-01 00:00:00
abstract：:Biofilms are the most widely distributed and successful microbial modes of life. The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress. Bacteria living in cold environments...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.09.010
更新日期：2019-01-01 00:00:00
abstract：:We previously reported that inactivation of a universally conserved dimethyl adenosine transferase (KsgA) attenuates virulence and increases sensitivity to oxidative and osmotic stress in Salmonella Enteritidis. Here, we show a role of KsgA in cell-envelope fitness as a potential mechanism underlying these phenotypes ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.08.009
更新日期：2018-11-01 00:00:00
abstract：:Given the current trend towards reducing the use of chemical controls in agriculture, microbial resources such as plant endophytes are being intensively investigated for traits that are conducive to plant protection. Among the various important target pathogens, Fusarium graminearum is a fungal pathogen of cereal crop...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126650
更新日期：2021-02-01 00:00:00
abstract：:Iron-sulfur ([Fe-S]) cluster is an essential cofactor of proteins involved in various physiological processes including cellular defense against oxidative stress. In Xanthomonas campestris pv. campestris (Xcc), IscR plays a negative role in regulation of the transcription of [Fe-S] assembly genes, iscR-sufBCDS. The ex...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2014.08.004
更新日期：2015-01-01 00:00:00
abstract：:Changes in microbial numbers and activities in a soil in response to bentazon applied at 10 and 100 ppm were studied after 4 and 30 weeks of incubation in laboratory conditions. As regards the eight general and functional microbial groups studied (aerobic and anaerobic bacteria, fungi, aerobic and anaerobic N2-fixing ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(96)80064-4
更新日期：1996-03-01 00:00:00
abstract：:The emergence of resistance among fungal phytopathogens poses a biggest threat across the world. Streptomyces are a group of spore-forming Gram + ve bacteria and prolific producers of secondary bioactive metabolites which have been used as biocontrol agents against phytopathogens and also known for plant growth promot...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126478
更新日期：2020-08-01 00:00:00
abstract：:Bitespiramycin (biotechnological spiramycin, Bsm) is a new 16-membered macrolide antibiotic produced by Streptomyces spiramyceticus WSJ-1 integrated exogenous genes. The gene cluster for Bsm biosynthesis consists of two parts: spiramycin biosynthetic gene cluster (92 kb) and two exogenous genes including 4"-O-isovaler...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126532
更新日期：2020-11-01 00:00:00
abstract：:The genes that encode the alpha and beta subunits of protocatechuate 3,4-dioxygenase (3,4-PCD [EC 1.13.11.3]) were cloned from a Pseudomonas marginata genomic library. These genes pcaG and pcaH, were found when screening the library for hydrolase genes. The two open reading frames of the PCD genes could be identified ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(96)80004-8
更新日期：1996-12-01 00:00:00
abstract：:Endophytic bacteria are the plant beneficial bacteria that thrive inside plants and can improve plant growth under normal and challenging conditions. They can benefit host plants directly by improving plant nutrient uptake and by modulating growth and stress related phytohormones. Indirectly, endophytic bacteria can i...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2019.02.001
更新日期：2019-04-01 00:00:00
abstract：:In this study, we evaluated the efficiency of six isolates of Saccharomyces cerevisiae in controlling Colletotrichum acutatum, the causal agent of postbloom fruit drop that occur in pre-harvest citrus. We analyzed the mechanisms of action involved in biological control such as: production of antifungal compounds, nutr...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.04.003
更新日期：2015-06-01 00:00:00
abstract：:The progression of life in all forms is not only dependent on agricultural and food security but also on the soil characteristics. The dynamic nature of soil is a direct manifestation of soil microbes, bio-mineralization, and synergistic co-evolution with plants. With the increase in world's population the demand for ...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2017.08.016
更新日期：2018-01-01 00:00:00
abstract：:The BIOLOG system was used to generate knowledge of enterococci populations found in fresh and dry manure of livestock (cattle (Bos taurus), horse (Equus caballus), and sheep (Ovis aires)). Six-hundred and forty Enterococcus isolates from the host sources were observed as a combined fresh manure unit and a combined dr...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.11.017
更新日期：2009-01-01 00:00:00
abstract：:Shewanella putrefaciens was grown on a series of ten alternate compounds as sole terminal electron acceptor. Each cell type was analyzed for Fe(III) reduction activity, absorbance maxima in reduced-minus-oxidized difference spectra and heme-containing protein content. High-rate Fe(III) reduction activity, pronounced d...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(00)80042-7
更新日期：2000-07-01 00:00:00
abstract：:Twenty-nine rhizobial isolates from root nodules of the wild Legumes Astragalus, Lespedeza and Hedysarum growing in the northwestern region of China, were characterized by numerical taxonomy, RFLP and sequencing of PCR-amplified 16S rDNA genes, and cross-nodulation with selected Legume species. Based on the results fr...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.09.005
更新日期：2008-01-01 00:00:00
abstract：:The pathogenicity of a new China variety of Metarhizium anisopliae (M. anisopliae var. dcjhyium) against the subterranean termite Odontotermes formosanus and the effect of the fungal fermented solution on hemolymph intracellular calcium were studied in laboratory. Conidia from the M. anisopliae var. dcjhyium were high...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.11.009
更新日期：2009-01-01 00:00:00
abstract：:Actinomadura hibisca P157-2 produces potent antifungal antibiotic pradimicins. To enhance pradimicin production, ACCase from Streptomyces coelicolor and two regulatory genes metK1-sp and afsR-sp from Streptomyces peuticus were overexpressed into A. hibisca using an integration vector pSET152 under the control of the s...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2011.02.007
更新日期：2011-12-20 00:00:00
abstract：:β-lactamase inhibitors are potent synergistic drugs to deteriorate the multidrug-resistant bacteria. Here, we report the β-lactamase inhibitory ability of kalafungin isolated from a marine sponge derived Streptomyces sp. SBRK1. The IC50 value of the kalafungin was calculated as 225.37 ± 1.95 μM against β-lactamase. Th...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126666
更新日期：2021-03-01 00:00:00
abstract：:Annual ryegrass is a fast-growing cool-season grass broadly present in the Portuguese "montado", a typically Mediterranean agro-forestry-pastoral ecosystem. A culture-dependent approach was used to investigate natural associations of this crop with potentially beneficial bacteria, aiming to identify strains suitable f...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.12.010
更新日期：2014-09-01 00:00:00
abstract：:A putative sigma factor gene, orf21, was disrupted or overexpressed in the wild-type clavulanic acid (CA) producer Streptomyces clavuligerus NRRL3585 and characterized. An orf21 mutant (Streptomyces clavuligerus HN14) of S. clavuligerus was obtained by insertional inactivation via double-crossover. Although there was ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.07.005
更新日期：2011-07-20 00:00:00
abstract：:Chaperone DnaK and its co-chaperone DnaJ plays various essential roles such as in assisting in the folding of nascent peptides, preventing protein aggregation and maintaining cellular protein homeostasis. Global transcriptional changes in vivo associated with deletion of dnaKJ were monitored using DNA microarray to el...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.03.001
更新日期：2016-05-01 00:00:00
abstract：:The biggest non-tree perennial plant species endemic to Mexico were called metl in the Nahua culture; during colonial times, renamed with the Antillean word maguey. Carl von Linné finally renamed them as Agave, a Greek-Latin root word meaning admirable. Since pre-Columbian times, one of the major products obtained fro...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126593
更新日期：2020-12-01 00:00:00
abstract：:DrrC is a DNA-binding protein of Streptomyces peucetius that provides self-resistance against daunorubicin, the antibiotic produced by the organism. DrrC was expressed in E.coli and purified by using N-terminal MBP-tag which retained DNA-binding property in spite of the tag. Mobility shift assay confirmed the interact...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.05.002
更新日期：2017-09-01 00:00:00
abstract：:Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) expressing Panton-Valentine leukocidin (PVL) have been characterized worldwide, but it has not been done in Egypt. In this study, we analyzed the molecular characteristics of PVL+CA-MRSA in Egypt, compared their genetic patterns with that of PVL+...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.03.005
更新日期：2010-02-28 00:00:00
abstract：:The organophosphorus hydrolase (OPH) of Nocardia sp. strain B-1 is capable of hydrolyzing organophosphate insecticides such as coumaphos and parathion. The 40,000 dalton OPH and the responsible gene (termed adpB) have been previously isolated and described. OPH activity in Nocardia strain B-1 is spontaneously lost at ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/s0944-5013(98)80003-7
更新日期：1998-11-01 00:00:00
abstract：:In recent years, many studies have shown that phytochemicals exert their antibacterial activity through different mechanisms of action, such as damage to the bacterial membrane and suppression of virulence factors, including inhibition of the activity of enzymes and toxins, and bacterial biofilm formation. In this rev...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2016.12.003
更新日期：2017-03-01 00:00:00
