Abstract：
:Vibrio vulnificus is a serious bacterial pathogen for humans and aquatic animals. We developed a rapid, sensitive and specific identification method for V. vulnificus using loop-mediated isothermal amplification (LAMP) technique. A set of primers, composed of two outer primers and two inner primers, was designed based on the cytolysin gene sequence of V. vulnificus. The LAMP reaction was processed in a heat block at 65 degrees C for 60 min. The amplification products were detected by visual inspection using SYBR Green I, as well as by electrophoresis on agarose gels. Our results showed that the LAMP reaction was highly specific to V. vulnificus. This method was 10-fold more sensitive than conventional PCR. In conclusion, the LAMP assay was extremely rapid, simple, cost-effective, sensitive and specific for the rapid identification of V. vulnificus.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Ren CH,Hu CQ,Luo P,Wang QBdoi
10.1016/j.micres.2008.05.002subject
Has Abstractpub_date
2009-01-01 00:00:00pages
514-21issue
5eissn
0944-5013issn
1618-0623pii
S0944-5013(08)00029-3journal_volume
164pub_type
杂志文章abstract：:Colonization of micropropagated sugarcane plants by Gluconacetobacter diazotrophicus and Herbaspirillum sp. was confirmed by a dot-immunoblot assay. In all, a 45-day short-term and 180-day long-term experiments conducted on micropropagated sugarcane plants of Co 86032, a sugar rich popular variety in South India, indi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.08.007
更新日期：2006-01-01 00:00:00
abstract：:Among the 49 strains of moderately halophilic bacteria isolated from the salty environments of Iran, a Gram-positive coccus designated as strain QW6 showed high capacity in the removal of toxic oxyanions of tellurium in a wide range of culture medium factors including pH (5.5-10.5), temperature (25-45 degrees C), vari...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.07.010
更新日期：2008-01-01 00:00:00
abstract：:To evaluate the roles of blaIMP and blaTEM genes in the resistance of Serratia marcescens against beta-lactams and to find the spreading ways of these genes, 19 clinical isolates of imipenem-resistant Serratia marcescens were analyzed. Six strains bore blaIMP and blaTEM genes on a single plasmid, as confirmed by trans...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.06.005
更新日期：2007-01-01 00:00:00
abstract：:Pseudomonas aeruginosa is one of the most common pathogens associated with nosocomial infections and a great concern to immunocompromised individuals especially in the cases of cystic fibrosis, AIDS and burn wounds. The pathogenicity of P. aeruginosa is largely directed by the quorum sensing (QS) system. Hence, QS may...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.05.001
更新日期：2019-09-01 00:00:00
abstract：:There is a growing interest to find alternate bioresources for production of ethanol, apart from cane/sugar beet molasses and starchy crops like sweet sorghum, cassava and sweet potato. Mahula (Madhuca latifolia L.) is a forest tree abundantly available in the Indian subcontinent and its flowers are very rich in ferme...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.01.009
更新日期：2007-01-01 00:00:00
abstract：:Sulfate related water quality and trophic status are crucial to operation of water diversion. Though the sulfur geochemistry in the lake sediment have been well studied, the effective indicator of surrounding environment conditions related to sulfur in river-lake systems are still unknown. In this study, Dongping Lake...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126453
更新日期：2020-06-01 00:00:00
abstract：:Soybean (Glycine max L.) is among the most economically important legumes that provide more than 1/4 of food (for man) and animal feed. However, its yield is comparatively low, most especially under drought stress. The aim of this study therefore was to assess the ability of Rhizobium spp. and mycorrhizal fungi to enh...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126640
更新日期：2021-01-01 00:00:00
abstract：:DrrC is a DNA-binding protein of Streptomyces peucetius that provides self-resistance against daunorubicin, the antibiotic produced by the organism. DrrC was expressed in E.coli and purified by using N-terminal MBP-tag which retained DNA-binding property in spite of the tag. Mobility shift assay confirmed the interact...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.05.002
更新日期：2017-09-01 00:00:00
abstract：:The orphan histidine kinase (HK) from Streptomyces peucetius ATCC 27952 (ohkAsp) was found to be implicated in the regulation of doxorubicin (DOX)/daunorubicin (DNR) biosynthesis, self-defense and developmental attributes. OhkAsp is a homolog of OhkA from Streptomyces coelicolor and Streptomyces avermitilis (with 73 a...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.05.007
更新日期：2018-09-01 00:00:00
abstract：:A strain of Fusarium moniliforme isolated from a tropical mangrove ecosystem near Mumbai, India and deposited in the National Collection of Industrial Microorganisms (NCIM) as F. moniliforme NCIM 1276. The organism produced a single extracellular polygalacturonase (PG I) [EC 3.2.1.15] at pH 5 and a single pectate lyas...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.06.002
更新日期：2004-01-01 00:00:00
abstract：:We have cloned a laccase gene fragment isolated from a Trametes versicolor strain in Korea. It showed high similarity in nucleotide sequences when compared with other fungal laccases. TNT (2,4,6-trinitrotoluene), a widely used explosive, was transformed rapidly by T. versicolor. When TNT and its catabolic intermediate...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.12.001
更新日期：2006-01-01 00:00:00
abstract：:Verticillium dahliae is a significant pathogen in cucurbit cropping systems for which there are limited control options outside of soil fumigation. Endophytes, fungi and bacteria that live within plant hosts without impacting the host negatively, have exhibited antagonism to V. dahliae. The objectives of this study we...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126535
更新日期：2020-11-01 00:00:00
abstract：:Western immunoblot analyses of whole cell lysates probed with a human specific monoclonal anti-topoisomerase IIalpha antibody identified a 190 kDa protein over expressed in the arsenite resistant Leishmania donovani strain. The crude nuclear extract of the resistant strain showed higher topoisomerase II-like enzyme ac...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00177
更新日期：2003-01-01 00:00:00
abstract：:Probiotics have been used to rebuild the antibiotic-induced dysfunction in gut microbiota, but whether the different strains of probiotics result in similar or reverse effects remains unclear. In this study, the different recovery effects of two cocktails (each contains four strains) of Lactobacillus and fructooligosa...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.04.001
更新日期：2017-07-01 00:00:00
abstract：:Araucaria angustifolia, a unique species of this genus that occurs naturally in Brazil, has a high socio-economic and environmental value and is critically endangered of extinction, since it has been submitted to intense predatory exploitation during the last century. Root-associated bacteria from A. angustifolia were...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2011.03.003
更新日期：2012-01-20 00:00:00
abstract：:Bacterial utilization of monomers is recognized as an important step in the biogeochemical cycling of organic matter. In this study we have compared the heterotrophic activity of bacterial communities from different micro-habitats within a salt marsh environment (Ria de Aveiro, Portugal) in order to establish spatial ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.02.001
更新日期：2005-01-01 00:00:00
abstract：:The use of biocontrol strains recently has become a popular alternative to conventional chemical treatments. A set of bacteria isolated from sugar beet rhizosphere and from roots and shoots of apple and walnut were evaluated for their potential to control sugar beet seedling damping-off caused by R. solani AG-4 and AG...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.06.011
更新日期：2016-11-01 00:00:00
abstract：:Salmonella Pullorum is a pathogen specific to birds that can cause Pullorum disease in young chickens and lead to considerable economic losses in the poultry industry. During transmission and infection, S. Pullorum will encounter various environmental stresses and host defenses. The stringent response is an important ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126685
更新日期：2021-01-05 00:00:00
abstract：:Extended spectrum beta-lactamases (ESBLs) usually associated with multiple drug resistance, including beta-lactam and non-beta-lactam antibiotics. This resistance can cause Limitation in the choice of drugs appropriate for using in clinical practice, especially in life-threatening infections. In this study we aimed to...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.10.001
更新日期：2005-01-01 00:00:00
abstract：:Clonostachys chloroleuca 67-1 (formerly C. rosea 67-1) is a potential biocontrol fungus active against various fungal plant pathogens. From transcriptome sequencing of 67-1 parasitizing sclerotia of Sclerotinia sclerotiorum, we identified the transcription factor-encoding gene crtf that is significantly up-regulated d...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.03.002
更新日期：2018-05-01 00:00:00
abstract：:The enolase EnoA1 of Lactobacillus plantarum is here shown to interact with human plasminogen (Plg). By sequence alignment of EnoA1 with Streptococcus pneumoniae and Bifidobacterium lactis enolases, we identified BS1 and BS2 Plg-binding sites. A structure prediction of EnoA1 showed lysine residues in position 255 (BS2...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2012.10.001
更新日期：2013-02-22 00:00:00
abstract：:Twenty-nine rhizobial isolates from root nodules of the wild Legumes Astragalus, Lespedeza and Hedysarum growing in the northwestern region of China, were characterized by numerical taxonomy, RFLP and sequencing of PCR-amplified 16S rDNA genes, and cross-nodulation with selected Legume species. Based on the results fr...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.09.005
更新日期：2008-01-01 00:00:00
abstract：:Microorganisms often suffer from oxidative stress created from nutrient starvation and environmental changes. Thioredoxin (Trx) and glutathione (GSH) pathways are believed critical in related protective functions. The roles of Trx in improving abiotic stress resistance in Trichoderma reesei are still unclear. In this ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2015.01.005
更新日期：2015-02-01 00:00:00
abstract：:Infection caused by enterotoxigenic Escherichia coli (ETEC) poses a serious health problem to children in developing countries. Colonization of the small intestinal mucosa by ETEC strains is mediated by antigenically specific fimbriae, also known as colonization factor antigens (CFA). The importance of this study aris...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(11)80027-3
更新日期：1995-11-01 00:00:00
abstract：:The coral-killing invasive sponge, Terpios hoshinota, causes extensive mortality to live corals and is a potential threat to reefs at different geographical locations. However, to date, the invasive mechanism remains largely unknown. In this study, we aimed to understand the bacterial competition between sponge and co...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126553
更新日期：2020-11-01 00:00:00
abstract：:Sponges harbor diverse prokaryotic and eukaryotic microbes. However, the nature of sponge-fungal association and diversity of sponge-derived fungi have barely been addressed. In this study, the cultivation-dependent approach was applied to study fungal diversity in the Hawaiian sponges Gelliodes fibrosa, Haliclona cae...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2007.07.002
更新日期：2009-01-01 00:00:00
abstract：:Systemic yeast infections are the Leading cause of mortality and morbidity in immunocompromized patients. Candida albicans, being the most frequently isolated fungal pathogen in these patients, can be divided into three genotypes (genotypes A, B and C) by 25S intron analysis. In our study, we found that molecular size...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2005.02.010
更新日期：2005-01-01 00:00:00
abstract：:Recent studies have shown that the canonical Shine-Dalgarno (SD)-anti-SD interaction is dispensable for the initiation of translation of certain mRNAs in Escherichia coli. Alternative non-SD sequences (located upstream from the initiation codon) and also downstream sequences ("downstream boxes") complementary to 16S r...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(98)80037-2
更新日期：1998-08-01 00:00:00
abstract：:Alcaligenes sp. strain O-1 inducibly deaminates 2-aminobenzenesulfonate (ABS) via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. This intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway (Scm). The complete pathwa...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.05.006
更新日期：2010-05-30 00:00:00
abstract：:Quorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic AHLs [N-...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.04.011
更新日期：2013-11-07 00:00:00
