Abstract：
:Pythium intermedium is known to play an important role in the carbon cycling of cool-temperate forest soils. In this study, a fast, precise and effective real-time PCR technique for estimating the population densities of P. intermedium from soils was developed using species-specific primers. Specificity was confirmed both with conventional PCR and real-time PCR. The detection limit (sensitivity) was determined and amplification standard curves were generated using SYBR Green II fluorescent dye. A rapid and accurate assay for quantification of P. intermedium in Takayama forest soils of Japan was developed using a combination of a new DNA extraction method and PCR primers were developed for real-time PCR. And the distribution of P. intermedium in forest soil was investigated with both soil plating method and the developed real-time PCR technique. This new technique will be a useful tool and can be applied to practical use for studying the role of Pythium species in forest and agricultural ecosystems.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Li M,Senda M,Komatsu T,Suga H,Kageyama Kdoi
10.1016/j.micres.2009.11.010subject
Has Abstractpub_date
2010-10-20 00:00:00pages
695-705issue
8eissn
0944-5013issn
1618-0623pii
S0944-5013(09)00113-Xjournal_volume
165pub_type
杂志文章abstract：:The endophytic bacteria were isolated from coffee roots and seeds in Vietnam and identified with 16S rDNA sequencing as belonging to the Actinobacteria, Firmicutes and Proteobacteria phyla with the Nocardia, Bacillus and Burkholderia as dominant genera, respectively. Out of the thirty genera recovered from Coffea cane...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126613
更新日期：2021-01-01 00:00:00
abstract：:Outer membrane protein U (OmpU) is a major porin from Vibrio alginolyticus and has been considered a vaccine candidate against infection by V. alginolyticus. After pre-incubated with polyclonal antibody against rOmpU, V. alginolyticus showed a 78% decrease in extracellular iron level, suggesting that interruption of O...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.126350
更新日期：2020-01-01 00:00:00
abstract：:Leukotoxins produced by staphylococci, especially Staphylococcus aureus, have long been considered important virulence determinants. The present assay examined 26 strains of staphylococci isolated from sheep for their ability to produce factors leukotoxic to polymorphonuclear leukocytes deriving from ovine mammary gla...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(97)80035-3
更新日期：1997-09-01 00:00:00
abstract：:Sestrins are highly conserved; stress inducible proteins that help maintain metabolic homeostasis and protect cells under stress conditions. They are up-regulated during stress and influence AMPK and mTOR pathways. Our objective was to find the role of Sestrin protein from Dictyostelium discoideum (Dd), a lower eukary...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.12.006
更新日期：2019-03-01 00:00:00
abstract：:Moderately halophilic bacteria are a kind of extreme environment microorganism that can tolerate moderate salt concentrations ranging from 0.5M to 2.5M. Here, via a metagenomic library screen, we identified four putative Na(+) transporters, designated H7-Nha, H16-Mppe, H19-Cap and H35-Mrp, from moderately halophilic c...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2012.06.004
更新日期：2013-02-22 00:00:00
abstract：:Mycobacterium tuberculosis Rv2140c is a function unknown conserved phosphatidylethanolamine-binding protein (PEBP), homologous to Raf kinase inhibitor protein (RKIP) in human beings. To delineate its function, we heterologously expressed Rv2140c in a non-pathogenic M. smegmatis. Quantitative phosphoproteomic analysis ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126615
更新日期：2021-01-01 00:00:00
abstract：:Although the use of crop-associated bacteria as biological control agents of fungal diseases has gained increasing interest, the biotechnological potential of forest tree-associated microbes and their natural products has scarcely been investigated. The objective of this study was to identify bacteria or bacterial pro...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126440
更新日期：2020-05-01 00:00:00
abstract：:In search of the optimal way to reduce the hazards of environmental contamination by formaldehyde (FD) and methanol the use of unconventional yeasts is proposed as exemplified by the methylotrophic yeast Hansenula polymorpha. In a very simplified environment of a model wastewater solution, H. polymorpha cells were abl...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(00)80002-6
更新日期：2000-03-01 00:00:00
abstract：:Alternaria species have been recognized as important cause of allergic diseases and are considered opportunistic pathogens for immunocompromised individuals. In order to assess the contribution of waters to the spread of Alternaria, we examined 390 water samples of various origin for the presence of these microorganis...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(00)80022-1
更新日期：2000-04-01 00:00:00
abstract：:The classical salicylaldehyde dehydrogenases found in naphthalene-degrading bacteria are denoted as NahF. In addition to NahF, NahV, and its corresponding gene nahV, were found here in multiple naphthalene-degrading bacteria isolated from industrial wastewater polluted with polycyclic aromatic hydrocarbons (PAHs). In ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2011.01.003
更新日期：2011-12-20 00:00:00
abstract：:Monodon baculovirls (MBV), external fouling organisms (EFO) and bacteria (especially Vibrio species) were monitored during 1996-1997 at nine different Penaeus monodon rearing hatcheries in India. Total cultivable heterotrophic bacteria, Vibrio-like-bacteria, presumptive Vibrio harveyi, Vibrio anguillarum, Vibrio vulni...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00208
更新日期：2003-01-01 00:00:00
abstract：:Central southern Mexico contains highly diverse legumes. In this study, nodule-associated bacteria (NAB) were isolated from wild legume nodules and from nodules on Phaseolus vulgaris plants used as a plant-trap in soils from the same areas as the wild legumes. The bacteria were identified through the 16S rRNA gene seq...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126522
更新日期：2020-10-01 00:00:00
abstract：:Quorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic AHLs [N-...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.04.011
更新日期：2013-11-07 00:00:00
abstract：:A polygalacturonase with a molecular mass of 74 kDa, an isoelectric point around pH 4.2 and pH--and temperature optima of 3.9 and 50 degrees C, respectively, was purified from a culture fluid of Penicillium frequentans. The enzyme was characterized as an exo-alpha-1,4-polygalacturonase (exo-PG I). Km and Vmax for sodi...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00127
更新日期：2002-01-01 00:00:00
abstract：:The bacterium Helicobacter pylori infects more than 50% of the world population and causes several gastroduodenal diseases, including gastric cancer. Nevertheless, we still need to explore some protein interactions that may be involved in pathogenesis. MreB, an actin homolog, showed some special characteristics in pre...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.04.008
更新日期：2017-08-01 00:00:00
abstract：:Biofilm formation of Group A Streptococcus (GAS) is recognized as an important virulent determinant. The present study reports the antibiofilm potential of seaweed (Gracilaria gracilis) surface associated Bacillus subtilis against GAS. Purification revealed 2,4-Di-tert-butyl-phenol (DTBP) as the active principle. DTBP...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2016.05.010
更新日期：2016-10-01 00:00:00
abstract：:Salmonella enterica serovar Enteritidis (S. Enteritidis, SE) is a major cause of foodborne diseases for humans. The completeness of the O-chain antigen of Lipopolysaccharide (LPS) determines whether a S. Enteritidis strain is smooth or rough. However, genes that are involved in the synthesis of LPS and rough-smooth va...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.05.001
更新日期：2018-01-01 00:00:00
abstract：:The mariner transposon family of Himar1 has been widely used for the random mutagenesis of bacteria to generate single insertions into the chromosome. Here, a versatile toolbox of mariner transposon derivatives was generated and applied to the functional genomics investigation of fish pathogen Edwardsiella piscicida. ...
journal_title：Microbiological research
pub_type： 杂志文章,收录出版
doi：10.1016/j.micres.2018.11.008
更新日期：2019-02-01 00:00:00
abstract：:A putative sigma factor gene, orf21, was disrupted or overexpressed in the wild-type clavulanic acid (CA) producer Streptomyces clavuligerus NRRL3585 and characterized. An orf21 mutant (Streptomyces clavuligerus HN14) of S. clavuligerus was obtained by insertional inactivation via double-crossover. Although there was ...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2010.07.005
更新日期：2011-07-20 00:00:00
abstract：:Shewanella putrefaciens was grown on a series of ten alternate compounds as sole terminal electron acceptor. Each cell type was analyzed for Fe(III) reduction activity, absorbance maxima in reduced-minus-oxidized difference spectra and heme-containing protein content. High-rate Fe(III) reduction activity, pronounced d...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/S0944-5013(00)80042-7
更新日期：2000-07-01 00:00:00
abstract：:We have now entered in the post-genomic era, where we have knowledge of plethora of fungal genomes and cutting edge technology is available to study global mRNA, protein and metabolite profiles. These so-called 'omic' technologies (transcriptomics, proteomics and metabolomics) provide the possibility to characterize p...
journal_title：Microbiological research
pub_type： 杂志文章,评审
doi：10.1016/j.micres.2007.06.006
更新日期：2007-01-01 00:00:00
abstract：:A strain of Fusarium moniliforme isolated from a tropical mangrove ecosystem near Mumbai, India and deposited in the National Collection of Industrial Microorganisms (NCIM) as F. moniliforme NCIM 1276. The organism produced a single extracellular polygalacturonase (PG I) [EC 3.2.1.15] at pH 5 and a single pectate lyas...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2004.06.002
更新日期：2004-01-01 00:00:00
abstract：:Lactobacillus rhamnosus DSM 14870 and Lactobacillus gasseri DSM 14869 were previously isolated from the vaginal epithelial cells (VEC) of healthy women and selected for the development of the vaginal EcoVag® probiotic capsules. EcoVag® was subsequently shown to provide long-term cure and reduce relapse of bacterial va...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2017.08.003
更新日期：2017-12-01 00:00:00
abstract：:The saline pond microalga, Dunaliella salina (Dunal) Teod. maintained in De Walne's (basal) medium under laboratory conditions was confirmed by amplifying the chromosomal DNA of the microalga by PCR with specific primers MA1 and MA2. Seaweed extracts obtained from Sargassum wightii and Ulva lactuca were amended separa...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2006.03.006
更新日期：2007-01-01 00:00:00
abstract：:Selected isolates of Pseudomonas fluorescens (Pf1-94, Pf4-92, Pf12-94, Pf151-94 and Pf179-94) and chemical resistance inducers (salicylic acid, acetylsalicylic acid, DL-norvaline, indole-3-carbinol and lichenan) were examined for growth promotion and induced systemic resistance against Fusarium wilt of chickpea. A mar...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1078/0944-5013-00202
更新日期：2003-01-01 00:00:00
abstract：:Increased intestinal absorption of oxalate causes hyperoxaluria, a major risk factor for kidney stone disease. Intestinal colonization of recombinant probiotic bacteria expressing oxalate-degrading gene (OxdC) is an effective therapeutic option for recurrent calcium oxalate (CaOx) stone disease. Therefore, we aimed to...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2018.06.009
更新日期：2018-10-01 00:00:00
abstract：:The effectiveness of aqueous extracts of various medicinal plants in detoxification of aflatoxin B1 (AFB1) was tested in vitro by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the maximum degradation...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2013.07.008
更新日期：2014-04-01 00:00:00
abstract：:Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) expressing Panton-Valentine leukocidin (PVL) have been characterized worldwide, but it has not been done in Egypt. In this study, we analyzed the molecular characteristics of PVL+CA-MRSA in Egypt, compared their genetic patterns with that of PVL+...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2009.03.005
更新日期：2010-02-28 00:00:00
abstract：:Two morphologically different bacteriophages were isolated from the river and soil samples from various locations of Maharashtra, India against the phytopathogen Pseudomonas sp. that was recently reported to cause a new bacterial blight of pomegranate. Both the phages belonged to the order Caudovirales representing th...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2019.126300
更新日期：2019-11-01 00:00:00
abstract：:Soybean (Glycine max L.) is among the most economically important legumes that provide more than 1/4 of food (for man) and animal feed. However, its yield is comparatively low, most especially under drought stress. The aim of this study therefore was to assess the ability of Rhizobium spp. and mycorrhizal fungi to enh...
journal_title：Microbiological research
pub_type： 杂志文章
doi：10.1016/j.micres.2020.126640
更新日期：2021-01-01 00:00:00
