Abstract:
:The operation of wastewater treatment technologies depends on a combination of physical, chemical and biological factors. Microorganisms present in wastewater treatment plants play essential roles in the degradation and removal of organic waste and xenobiotic pollutants. Several microorganisms have been used in complementary treatments to process effluents rich in fats and oils. Microbial lipases have received significant industrial attention because of their stability, broad substrate specificity, high yields, and regular supply, as well as the fact that the microorganisms producing them grow rapidly on inexpensive media. In Colombia, bacterial community studies have focused on populations of cultivable nitrifying, heterotrophic and nitrogen-fixing bacteria present in constructed wetlands. In this study, culture-dependent methods, culture-independent methods (TTGE, RISA) and enzymatic methods were used to estimate bacterial diversity, to monitor temporal and spatial changes in bacterial communities, and to screen microorganisms that presented lipolytic activity. The dominant microorganisms in the Wastewater Treatment Plant (WWTP) examined in this study belonged to the phyla Firmicutes, Proteobacteria and Bacteroidetes. The enzymatic studies performed indicated that five bacterial isolates and three fungal isolates possessed the ability to degrade lipids; additionally, the Serratia, Kosakonia and Mucor genera presented lipase-mediated transesterification activity. The implications of these findings in regard to possible applications are discussed later in this paper. Our results indicate that there is a wide diversity of aerobic Gram-negative bacteria inhabiting the different sections of the WWTP, which could indicate its ecological condition, functioning and general efficiency.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Silva-Bedoya LM,Sánchez-Pinzón MS,Cadavid-Restrepo GE,Moreno-Herrera CXdoi
10.1016/j.micres.2016.08.006subject
Has Abstractpub_date
2016-11-01 00:00:00pages
313-325eissn
0944-5013issn
1618-0623pii
S0944-5013(16)30539-0journal_volume
192pub_type
杂志文章abstract::The progression of life in all forms is not only dependent on agricultural and food security but also on the soil characteristics. The dynamic nature of soil is a direct manifestation of soil microbes, bio-mineralization, and synergistic co-evolution with plants. With the increase in world's population the demand for ...
journal_title:Microbiological research
pub_type: 杂志文章,评审
doi:10.1016/j.micres.2017.08.016
更新日期:2018-01-01 00:00:00
abstract::β-lactamase inhibitors are potent synergistic drugs to deteriorate the multidrug-resistant bacteria. Here, we report the β-lactamase inhibitory ability of kalafungin isolated from a marine sponge derived Streptomyces sp. SBRK1. The IC50 value of the kalafungin was calculated as 225.37 ± 1.95 μM against β-lactamase. Th...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126666
更新日期:2021-03-01 00:00:00
abstract::The endophytic bacteria were isolated from coffee roots and seeds in Vietnam and identified with 16S rDNA sequencing as belonging to the Actinobacteria, Firmicutes and Proteobacteria phyla with the Nocardia, Bacillus and Burkholderia as dominant genera, respectively. Out of the thirty genera recovered from Coffea cane...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126613
更新日期:2021-01-01 00:00:00
abstract::The aim of this study was to assess the potential of lactic acid bacteria to inhibit the outgrowth of some common food-spoiling fungi. Culture supernatants of 17 Lactic acid bacterial strains as well as of three commercial probiotic cultures were evaluated for antifungal activity using an agar-diffusion method. The me...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2004.07.002
更新日期:2004-01-01 00:00:00
abstract::Extracellular proteases from 8 Antarctic psychrotolerant Pseudomonas sp. strains were purified and characterised. All of them are neutral metalloproteases, have an apparent molecular mass of 45kDa, optimal activity at 40 degrees C and pH 7-9, retaining significant activity at pH 5-11. With the exception of P96-18, whi...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2004.03.001
更新日期:2004-01-01 00:00:00
abstract::"Bryndza" is a traditional Slovak dairy product (type of soft cheese) made from sheep cheese which was ripened for 14 days. Because its manufacture, transporting and/or storing represent conditions which facilitate contamination, the effect of enterocin CCM4231 in "bryndza" was investigated with the aim to reduce the ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1078/0944-5013-00078
更新日期:2001-01-01 00:00:00
abstract::Actinomycetes have been isolated from three Brazilian tropical soils. The dispersion and differential centrifugation procedure revealed count values 1.5 to 5.0 times greater than those obtained by the conventional dilution plate technique for all soils and media tested. Eighteen strains, promising for biotechnological...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/S0944-5013(01)80007-0
更新日期:2001-03-01 00:00:00
abstract::The wild-type yeast Saccharomyces cerevisiae (S. cerevisiae) is able to export less than 1 percent of the protein to be secreted. The reasons for retention of most of the secretory proteins on the cell surface of S. cerevisiae are unknown. Recently, temperature-sensitive (ts) mutants of S. cerevisiae showing an overse...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1078/0944-5013-00067
更新日期:2001-01-01 00:00:00
abstract::Nidus Vespae (honeycomb) is a kind of traditional Chinese medicine that has been demonstrated to inhibit the growth and acid-production of oral cariogenic bacteria. Subsequent studies showed that the chloroform/methanol (Chl/MeOH) chemical extraction of Nidus Vespae was the most effective inhibitor of growth and acido...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2011.03.002
更新日期:2012-01-20 00:00:00
abstract::Studies on the microbial ecology of gut microbiota in bats are limited and such information is necessary in determining the ecological significance of these hosts. Short-nosed fruit bats (Cynopterus brachyotis brachyotis) are good candidates for microbiota studies given their close association with humans in urban are...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2013.04.001
更新日期:2013-10-01 00:00:00
abstract::The APSES family, comprising of the transcriptional regulators Asm1p, Phd1p, Sok2p, Efg1p, and StuA, is found exclusively in fungi and has been reported to control several cellular processes in these organisms. However, its function in dermatophytes has not yet been completely understood. Here, we generated two null m...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126592
更新日期:2020-12-01 00:00:00
abstract::Probiotics have been used to rebuild the antibiotic-induced dysfunction in gut microbiota, but whether the different strains of probiotics result in similar or reverse effects remains unclear. In this study, the different recovery effects of two cocktails (each contains four strains) of Lactobacillus and fructooligosa...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2017.04.001
更新日期:2017-07-01 00:00:00
abstract::Penicillium freii (Lund and Frisvad 1994) mutants deficient in the synthesis of xanthomegnin were isolated. In vitro translated mRNA populations from selected radiation induced mutants and naturally occurring P. freii strains not able to produce xanthomegnin were examined by 2-dimensional polyacrylamide gel electropho...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/S0944-5013(96)80026-7
更新日期:1996-08-01 00:00:00
abstract::The complete genomic DNA sequence was determined for one of the gene for the elongation factor 1 alpha (TEF), isolated from the zygomycete Absidia glauca. Sequence comparison with TEF genes from other fungi show the highest similarity to TEF-genes of the closely related zygomycete Mucor racemosus (Sundstrom et al. 198...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/s0944-5013(11)80035-2
更新日期:1995-03-01 00:00:00
abstract::The organophosphorus hydrolase (OPH) of Nocardia sp. strain B-1 is capable of hydrolyzing organophosphate insecticides such as coumaphos and parathion. The 40,000 dalton OPH and the responsible gene (termed adpB) have been previously isolated and described. OPH activity in Nocardia strain B-1 is spontaneously lost at ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/s0944-5013(98)80003-7
更新日期:1998-11-01 00:00:00
abstract::Alcaligenes sp. strain O-1 inducibly deaminates 2-aminobenzenesulfonate (ABS) via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. This intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway (Scm). The complete pathwa...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2009.05.006
更新日期:2010-05-30 00:00:00
abstract::Staphylococcus saprophyticus is a gram-positive microorganism responsible for urinary tract infections (UTIs). Although some virulence factors are characterized, such as urease, autolysins, adhesins and hemagglutinins, large-scale proteomic studies have not been performed within this species. We performed the characte...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2018.08.008
更新日期:2018-11-01 00:00:00
abstract::The BIOLOG system was used to generate knowledge of enterococci populations found in fresh and dry manure of livestock (cattle (Bos taurus), horse (Equus caballus), and sheep (Ovis aires)). Six-hundred and forty Enterococcus isolates from the host sources were observed as a combined fresh manure unit and a combined dr...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.11.017
更新日期:2009-01-01 00:00:00
abstract::We have now entered in the post-genomic era, where we have knowledge of plethora of fungal genomes and cutting edge technology is available to study global mRNA, protein and metabolite profiles. These so-called 'omic' technologies (transcriptomics, proteomics and metabolomics) provide the possibility to characterize p...
journal_title:Microbiological research
pub_type: 杂志文章,评审
doi:10.1016/j.micres.2007.06.006
更新日期:2007-01-01 00:00:00
abstract::Signal recognition particle (SRP) mediates targeting of proteins to appropriate cellular compartments, which is an important process in all living organisms. In prokaryotes, SRP consists of Ffh, a protein, and 4.5S RNA that recognizes signal peptide emerging from ribosomes. The SRP (Ffh) of one the most successful int...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2012.03.002
更新日期:2012-10-12 00:00:00
abstract::Flavin adenine dinucleotide (FAD)-binding proteins play a vital role in energy transfer and utilization during fungal growth and mycelia aggregation. We sequenced the genome of Volvariella volvacea, an economically important edible fungus, and discovered 41 genes encoding FAD-binding proteins. Gene expression profiles...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2013.02.009
更新日期:2013-10-01 00:00:00
abstract::Selected isolates of Pseudomonas fluorescens (Pf1-94, Pf4-92, Pf12-94, Pf151-94 and Pf179-94) and chemical resistance inducers (salicylic acid, acetylsalicylic acid, DL-norvaline, indole-3-carbinol and lichenan) were examined for growth promotion and induced systemic resistance against Fusarium wilt of chickpea. A mar...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1078/0944-5013-00202
更新日期:2003-01-01 00:00:00
abstract::Kineococcus radiotolerans is a Gram-positive, radio-resistant bacterium isolated from a radioactive environment. The small noncoding RNAs (sRNAs) in bacteria are reported to play roles in the immediate response to stress and/or the recovery from stress. The analysis of K. radiotolerans transcriptome sequencing results...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2016.06.001
更新日期:2016-11-01 00:00:00
abstract::Seeds are reservoirs of beneficial and harmful microorganism that modulates plant growth and health. Here, we access seed to seedling bacteriome assembly modified by seed-disinfection and the underlined effect over maize germination performance and root-seedlings microbial colonization. Seed-disinfection was performed...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126643
更新日期:2021-02-01 00:00:00
abstract::One strain of Lactobacillus acidophilus was found to produce a bacteriocin-like substance in the culture filtrate. The substance was produced in a growth-associated manner, showed heat stability at neutral and acidic pH and exhibited antibacterial activity against various species of Lactobacillus including L. acidophi...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1078/0944-5013-00190
更新日期:2003-01-01 00:00:00
abstract::The saline pond microalga, Dunaliella salina (Dunal) Teod. maintained in De Walne's (basal) medium under laboratory conditions was confirmed by amplifying the chromosomal DNA of the microalga by PCR with specific primers MA1 and MA2. Seaweed extracts obtained from Sargassum wightii and Ulva lactuca were amended separa...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.03.006
更新日期:2007-01-01 00:00:00
abstract::Hirsutella rhossiliensis OWVT-1 has substantial potential as a biocontrol agent against plant-parasitic nematodes. Serine proteases have emerged as a potentially useful factor in the nematode-fungus interactions. When grown in liquid culture with the nematode Panagrellus redivivus as the sole nitrogen source, an extra...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2009.01.003
更新日期:2009-01-01 00:00:00
abstract::The emergent pathogen Candida glabrata differs from other yeasts because it assimilates only two sugars, glucose and the disaccharide trehalose. Since rapid identification tests are based on the ability of this yeast to rapidly hydrolyze trehalose, in this work a biochemical and molecular characterization of trehalose...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.06.008
更新日期:2015-10-01 00:00:00
abstract::Plant growth-promoting rhizobacteria promote plant growth by direct and indirect mechanisms. We isolated twelve bacterial strains showing different degrees of phosphate solubilizing activity from maize rhizosphere. Four isolates solubilized over 300 μg mL⁻¹ phosphate from insoluble Ca₃(PO₄)₂, with isolate SCAUK0330 so...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2013.07.003
更新日期:2014-01-20 00:00:00
abstract::The aim of the present study, performed on typical Argiudolls in a natural reserve with little or no anthropic impact, was to characterize the fungous biomineralizing process of calcium oxalate crystals in organic horizons of the soil. The chosen sites possessed different plant cover, identified as acacia woods and gr...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1078/0944-5013-00083
更新日期:2001-01-01 00:00:00