Abstract:
:Soluble guanylyl cyclase is a heterodimeric (alpha, beta) enzyme generating the second messenger, cGMP, upon activation by the gaseous messenger, nitric oxide. The occurrence and distribution of alpha 1-, alpha 2-, beta 1- and beta 2-subunits were investigated in trigeminal and dorsal root ganglia on the mRNA and the protein level. Reverse transcription PCR analysis demonstrated mRNA coding for alpha 1-, alpha 2-, and beta 1-subunits in guinea-pig trigeminal and dorsal root ganglia. In agreement with these data, immunoreactivity to the alpha 1-subunit was found in satellite and Schwann cells, while alpha 2-subunit immunoreactivity was localized to axons of large diameter. The distribution of the beta 1-subunit could not be studied on the protein level since the antiserum was ineffective in immunohistochemistry. However, previous studies and the RT-PCR data argue in favour of alpha 1/beta 1-and alpha 2/beta 1-heterodimerization and colocalization. In both species, beta 2-subunit immunoreactivity was confined to neuronal perikarya, primarily of large diameter. Although these results were obtained with two different antibodies directed against different epitopes, the corresponding mRNA could not be detected by RT-PCR analysis. The reason for this discrepancy remains unclear, at present, but could be explained by a variant beta 2- or highly homologous as yet unidentified beta-subunit. This study demonstrates the presence of soluble guanylyl cyclase in sensory ganglia with a differential, cell type-specific distribution of the individual subunits.
journal_name
Brain Resjournal_title
Brain researchauthors
Kummer W,Behrends S,Schwarzlmüller T,Fischer A,Koesling Ddoi
10.1016/0006-8993(96)00097-2subject
Has Abstractpub_date
1996-05-20 00:00:00pages
191-5issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(96)00097-2journal_volume
721pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::The short and long-term effects of systemic administration of kainic acid to immature animals were studied in rats. Kainic acid was administered systemically to rats of 1-30 days of age. The rats were monitored for both behavioral and EEG changes. To study the effects of kainic acid on seizure susceptibility, status e...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(88)90066-1
更新日期:1988-03-01 00:00:00
abstract::Somatosensory information has been thought to ascend from the spinal cord to limbic areas of the telencephalon through indirect, multisynaptic pathways. We now report that injections of Fluoro-gold into either the nucleus accumbens or the septal nuclei labeled hundreds of neurons in the spinal cord. These and our rece...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90981-5
更新日期:1989-09-11 00:00:00
abstract::Examination of glycolmethacrylate embedded olfactory bulbs of normal rats revealed that the granule cells of the accessory olfactory bulb were dissimilar from the majority of main olfactory bulb granule cells. Cells from these structures can be discriminated on the basis of spherical form. nuclear diameter, nucleoplas...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90711-9
更新日期:1982-03-25 00:00:00
abstract::Axon-to-myelin transfer of lipids precursors have been studied in the rabbit optic system by intraocular injection of [32P]orthophosphate, [14C]glycerol and [3H]glycerol. Choline and ethanolamine phosphoglycerides and myelin showed increasing [32P]-radioactivity between 7 and 21 days following injection, while [3H]- a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90136-1
更新日期:1983-06-20 00:00:00
abstract::Studies related to brain oscillations pervade the neuroscience literature. Functional correlates of oscillations in the delta, theta, alpha, beta and gamma frequency windows are interpreted in several ways. However, only a small number of reports indicate the need for overall interpretations by integrating all type of...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2008.06.102
更新日期:2008-10-15 00:00:00
abstract::This study tested the effect on intracranially injected cycloheximide (CHX), an inhibitor of protein synthesis, on facilitation of sexual receptivity in ovariectomized rats. The rats received 0.5 microgram estradiol benzoate (EB), s.c. once daily on days 8 through 12 after ovariectomy (OVX). Either CHX (in 0.5 microli...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)91050-7
更新日期:1981-10-19 00:00:00
abstract::Previous examination of binge cocaine self-administration and 2 week withdrawal from cocaine self-administration on ionotropic glutamate receptor subunit (iGluRs) protein levels revealed significant alterations in iGluR protein levels that differed between the mesocorticolimbic and nigrostriatal pathways. The present ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.09.051
更新日期:2005-12-07 00:00:00
abstract::Several lines of evidence support a role for nerve growth factor (NGF) in the sympathetic sprouting response that occurs following septal cholinergic denervation of the rat hippocampal formation. The present study was undertaken to compare the distribution of NGF-like immunoreactivity and the topography of sympathetic...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)01344-h
更新日期:1995-02-20 00:00:00
abstract::The excitatory amino acid L-glutamate injected into the nucleus of the solitary tract (NTS) in unanesthetized rats similar to peripheral chemoreceptor activation increases mean arterial pressure (MAP) and reduces heart rate. In this study, we investigated the effects of acute (1 day) and chronic (15 days) electrolytic...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.08.006
更新日期:2004-10-29 00:00:00
abstract::To evaluate roles of the sympathetic nervous system in enhancing glucose uptake in skeletal muscles in response to electrical stimulation of the ventromedial hypothalamic nucleus (VMH), the effects of guanethidine treatment and adrenal demedullation on the response were examined in rats anesthetized and injected with ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91085-5
更新日期:1994-06-27 00:00:00
abstract::In rats anaesthetised with alphaxalone/alphadolone, electrical stimulation in the dorsolateral part of the periaqueductal grey matter (PAG; 10 s trains of 1 ms pulses at 80 Hz, 40-80 microA) evoked a pressor response accompanied by tachycardia. Both components of the response were attenuated following microinjection o...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90702-1
更新日期:1994-07-18 00:00:00
abstract::In the present study, we investigated the possibility that MK-801 (dizocilpine), a noncompetitive N-methyl-D-aspartate (NMDA) receptor antagonist, owes its potent neuroprotective properties to calcium channel blocking ability rather than to its NMDA receptor antagonism. Rat hippocampal slices were exposed to a long hy...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00466-4
更新日期:1995-07-03 00:00:00
abstract::Intraventricular injections of carbachol, a cholinergic agonist, into free-running mice can cause phase dependent phase shifts, in both directions, in the circadian rhythm of wheel-running activity. These effects mimic the action of light in entraining the circadian pacemaker, and suggest that acetylcholine may play a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90059-7
更新日期:1981-05-11 00:00:00
abstract::Nodes of Ranvier in the central nervous system in mammals are characterized by the presence of perinodal astrocytic processes. This study examines the association between processes of radial glia and the axolemma at nodes of Ranvier in the spinal cord of the mature axolotl, an animal in which radial glia represent a l...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90595-m
更新日期:1991-05-17 00:00:00
abstract::Axonal loss and degeneration in multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE) have been suggested by brain imaging, pathological and axonal transport studies. Further elucidation of the processes and mechanisms of axonal degeneration in demyelinating diseases is therefore of potential impor...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01191-9
更新日期:1999-04-10 00:00:00
abstract::Sex differences in cocaine's mechanisms of action and behavioral effects have been widely reported. However, little is known about how sex influences intracellular signaling cascades involved with drug-environment associations. We investigated whether ERK/CREB intracellular responses in the mesocorticolimbic circuitry...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2013.04.060
更新日期:2013-07-03 00:00:00
abstract::A novel putative receptor for (3-8)angiotensin (AngIV) has recently been found in various tissues, including the brain. However, the localization of AngIV binding sites to specific types of brain cells has yet to be established. In this study tissue culture was used to determine the presence and characteristics of Ang...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00278-x
更新日期:1995-05-29 00:00:00
abstract::Recent studies have supported the existence of projections to the paraventricular and supraoptic nuclei of the hypothalamus that arise from non-catecholaminergic neurons in the nucleus of the solitary tract, whose terminal distribution is suggestive of interactions with both parvocellular and magnocellular neurosecret...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00760-n
更新日期:1995-10-02 00:00:00
abstract::Neuropeptides, including substance P (SP), calcitonin gene-related peptide (CGRP) and somatostatin (SS) in dorsal root ganglia (DRG) may play a role in neurogenic inflammation and pain transmission. Adrenal corticosteroids regulate neuropeptide synthesis in some areas of the CNS and may modulate neurogenic inflammatio...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91347-4
更新日期:1991-11-08 00:00:00
abstract::Emerging evidence that autophagy serves as a sweeper for toxic materials in the brain gives us new insight into the pathophysiology of neurodegenerative diseases. Autophagy is important for maintaining cellular homeostasis associated with metabolism. Some neurodegenerative diseases such as Alzheimer׳s and Parkinson׳s ...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2016.02.049
更新日期:2016-10-15 00:00:00
abstract::Using the horseradish peroxidase (HRP) retrograde tracing method, we have studied the topographical organization of the projection of the septal organ to the main olfactory bulb in rats varying in age from 22 fetal days up to 15 postnatal days. For all developmental stages studied, receptor cells of the septal organ h...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(88)90248-9
更新日期:1988-08-01 00:00:00
abstract::The changes in the excitability of lumbosacral spinothalamic neurons produced by activating afferents in the trigeminal nerve using electrical or mechanical stimuli was investigated in cats anesthetized with alpha-chloralose. In most spinothalamic neurons, weak electrical stimuli or step indentations of the skin of th...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(79)90776-5
更新日期:1979-11-16 00:00:00
abstract::Selected optic fibers were surgically deflected from one tectum onto the opposite host tectum which was denervated by eye enucleation. At 6-8 months, deflected fibers were labeled with horseradish peroxidase and the retinotopically inappropriate part of tectum was examined using electron microscopy. Numerous (labeled)...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(88)90058-2
更新日期:1988-02-01 00:00:00
abstract:BACKGROUND:Previous experimental work using in situ zymography has shown very early increased plasminogen activation in ischemic regions after 3 h of ischemia with and without reperfusion. The objective of the present study was to evaluate the time course and extent of plasminogen activation in long-term permanent foca...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02769-4
更新日期:2000-11-03 00:00:00
abstract::Trophic effects of neurturin, a member of the glial cell line-derived neurotrophic factor-family, have been demonstrated on mesencephalic dopaminergic neurons, suggesting its therapeutic potential for Parkinson's disease. This study was designed to test the neuroprotective and regenerative effects of an intrastriatal ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)02934-7
更新日期:2002-08-30 00:00:00
abstract::This study used microdialysis to monitor extracellular levels of 5-HT and its metabolite, 5-hydroxyindole acetic acid (5-HIAA) in the lateral hypothalamus (LH) and hippocampus of freely moving rats that had developed a CTA to a 2.5 mM saccharin solution (CS) following its pairing with illness induced by lithium chlori...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90551-6
更新日期:1991-08-09 00:00:00
abstract::The genetic architecture of amyotrophic lateral sclerosis (ALS) is being increasingly understood. In this far-reaching review, we examine what is currently known about ALS genetics and how these genes were initially identified. We also discuss the various types of mutations that might underlie this fatal neurodegenera...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2014.10.009
更新日期:2015-05-14 00:00:00
abstract::The acute stroke phase is a critical time frame used to evaluate stroke severity, therapeutic options, and prognosis while also serving as a major tool for the development of diagnostics. To further understand stroke pathophysiology and to enhance the development of treatments, our group developed a translational pig ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2020.146778
更新日期:2020-06-01 00:00:00
abstract::Ligation of CD27, a member of the tumor necrosis factor (TNF) receptor family, by its ligand CD70 is thought to be important in T cell activation, expansion and survival, B cell activation, and NK cell activation. We examined the role of CD70 in Theiler's murine encephalomyelitis virus-induced demyelinating disease (T...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.12.058
更新日期:2010-03-04 00:00:00
abstract::To understand the role of hypothalamic dopamine in thermoregulation, single unit activity was recorded in vitro, from constantly perfused tissue slices of rat preoptic area and anterior hypothalamus, PO/AH. The firing rate and thermosensitivity of individual PO/AH neurons were determined before, during and after tissu...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90364-0
更新日期:1984-07-23 00:00:00