Abstract:
:Previous lectin-histochemical and immunocytochemical investigations using fixed tissue revealed perineuronal nets as lattice-like accumulations of extracellular matrix proteoglycans at the surface of several types of neurons. In the present study, perineuronal nets in the rat brain were labelled for the first time in vivo by stereotaxic injections of biotinylated Wisteria floribunda agglutinin (Bio-WFA), as well as in vitro, by incubation of unfixed brain slices with the same lectin. Six days after Bio-WFA injections into the parietal cortex, medial septum, reticular thalamic nucleus and red nucleus, the lectin remaining bound to perineuronal nets was detected by streptavidin/biotinylated peroxidase complexes or red fluorescent Cy3-streptavidin, respectively. Double-fluorescence labelling showed that Bio-WFA applied in vivo reacted with the chondroitin sulphate proteoglycan immunoreactive perineuronal nets in the injection zone. Labelling of perineuronal nets in unfixed slices was obtained with either Cy3-tagged WFA or Bio-WFA and subsequent visualization by Cy3-streptavidin which confirmed the region-dependent distribution patterns and the structural characteristics of perineuronal nets known from histochemical studies. These results provide support for the role of extracellular matrix proteoglycans to maintain a considerable chemical and, probably, spatial heterogeneity of the extracellular space in vivo. The ability of in vivo and in vitro labelling may promote the functional characterization of the extracellular matrix in various brain structures including its species-dependent neuronal association patterns.
journal_name
Brain Resjournal_title
Brain researchauthors
Brückner G,Bringmann A,Köppe G,Härtig W,Brauer Kdoi
10.1016/0006-8993(96)00152-7subject
Has Abstractpub_date
1996-05-13 00:00:00pages
84-92issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(96)00152-7journal_volume
720pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Deltamethrin is a highly potent pyrethroid insecticide that causes hypersensitivity, choreoathetosis, tremors, and paralysis in mammals. It is known to modify the sodium channel in such a way as to prolong the tail current associated with step repolarization following a depolarizing pulse. Using the axial-wire voltage...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90879-e
更新日期:1992-07-03 00:00:00
abstract::Effects of intracranial self-stimulation of central 'punishment areas' were studied on an operant conditioning of vertical eye movements in the midpontine pretrigeminal cats as well as in the encéphale isolé cats. In 36 pretrigeminal cats, the ventromedial hypothalamus (VMH), basal amygdaloid nuclei (AMY), dorsal cent...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)91008-8
更新日期:1981-12-21 00:00:00
abstract::Oxygen therapy (OT) with hyperbaric oxygen (HBO) or normobaric hyperoxia (NBO) improves the oxygenation of penumbral tissue in experimental ischemic stroke. However, whether this results in the improvement of energy metabolism is unclear. We investigated the effect of both OTs on tissue acidosis and on ATP production....
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.07.064
更新日期:2011-09-30 00:00:00
abstract::Antibodies to dopamine beta-hydroxylase (anti-D beta H) were taken up by noradrenergic nerve terminals in the iris following attachment to D beta H, and were transported back to, and accumulated in, the superior cervical ganglion (SCG). Concurrent, or prior destruction of noradrenergic terminals with 6-hydroxydopamine...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)91097-7
更新日期:1984-06-03 00:00:00
abstract::Circular RNAs (circRNAs) are a class of covalently closed non-coding RNAs, and aberrant alteration of their expression patterns is studied in numerous diseases. This study aimed to investigate whether intracerebral hemorrhage (ICH) affected circRNA expression profiles in the rat brain. Adult male Sprague-Dawley rats w...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2019.146490
更新日期:2020-01-01 00:00:00
abstract::The triggering of ectopic action potentials (APs) at axon terminals in a chronic epileptic cobalt focus was investigated in thalamocortical (TC) neurons of rats under urethane anesthesia. TC cells which were in register with an active epileptic aggregate discharged bursts of 2-11 APs. According to the rules of the col...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90628-m
更新日期:1992-03-27 00:00:00
abstract::We have conducted studies in intact adult male rats, designed to examine the effect of a 3- or 7-day exposure to alcohol (EtOH) on the pituitary's response to corticotropin-releasing hormone (CRF) or stress, and on CRF expression in the hypothalamus. In a first series of experiments, rats exposed to EtOH vapors for 7 ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)91685-a
更新日期:1990-06-18 00:00:00
abstract::The central nucleus of the amygdala (CEA) is selectively involved in the passive component of the behavioral (immobility) and the accompanying parasympathetic response during conditioned, stressful environmental challenges. Vasopressinergic mechanisms in the brain seem to play a role in these stress responses. The eff...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91529-n
更新日期:1992-11-20 00:00:00
abstract::Neurotensin-producing perikarya and fibers have been identified in the ventral tegmental area of the rat, and recent microinjection studies indicate that neurotensin may function in the ventral tegmental area to regulate body temperature. In this study, the hypothermic response produced by intraventral tegmental injec...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90031-9
更新日期:1985-02-11 00:00:00
abstract::In the present study we have examined the effects of changes in potassium and calcium concentration on the early adaptation of the slowly adapting stretch receptor of the crayfish using intracellular recordings including the potential clamp technique. This was because previous studies had suggested that the early adap...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90409-3
更新日期:1985-06-10 00:00:00
abstract::Expression of the immediate-early gene c-fos, a marker of neuronal activation, was employed to localize brainstem neuronal populations functionally related to the expiration reflex (ER). Twelve spontaneously breathing, non-decerebrate, pentobarbital anesthetized cats were used. The level of Fos-like immunoreactivity (...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.09.064
更新日期:2007-12-12 00:00:00
abstract::The role of central delta-opioid receptors in the mediation of opioid reinforcement and endogenous reward processes was examined using a non-biased place-preference conditioning procedure. Intracerebroventricular (i.c.v.) administration of the selective delta-receptor agonist, [D-Pen2, D-Pen5]-enkephalin (DPDPE, 10.0-...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91667-2
更新日期:1987-12-15 00:00:00
abstract::Unilateral olfactory nerve section was performed in the mouse. Three biochemical markers of the olfactory chemoreceptor neurons: carnosine, carnosine synthetase activity and the olfactory marker protein, were measured in the olfactory bulb and epithelium. Parallel observations were made by light microscopy as well as ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(77)90703-x
更新日期:1977-08-19 00:00:00
abstract::The regional distribution of FLFQPQRFamide binding sites on fresh unfixed cryostate sections from post mortem specimens of human spinal cord and lower medulla oblongata was studied by quantitative autoradiographic methods using [125I]YLFQPQRFamide as ligand. Samples were taken from five cases who had died with no hist...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91531-8
更新日期:1994-01-07 00:00:00
abstract::Characteristics of neural corticosteroid receptors were studied in 51 adrenally-intact macaque monkeys using a modification of a corticosteroid receptor assay developed in this laboratory for rodent studies. Using cortisol as a ligand, two receptor subtypes could be distinguished and with similar Kd's to those observe...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91249-1
更新日期:1994-02-21 00:00:00
abstract::Subnanomolar concentrations of melatonin inhibit cyclic AMP and cyclic GMP accumulation in neonatal rat anterior pituitary stimulated in vitro with luteinizing-hormone releasing-hormone. Melatonin also inhibited forskolin-stimulated cyclic AMP accumulation in pars tuberalis. Inhibition of cyclic AMP accumulation is sp...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90129-7
更新日期:1989-12-25 00:00:00
abstract::Cholecystokinin-octapeptide-like-immunoreactivity (CCK-8-LI) is demonstrable by the indirect immunofluorescent technique in cerebral cortical neurons cultured from fetal rats. Prominent and widespread staining is seen in widely ramifying cellular processes throughout the cultures. Staining in neuronal cell bodies is e...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)91456-2
更新日期:1984-02-13 00:00:00
abstract::Intracerebroventricular (i.c.v.) infusion of sodium in rats increases cerebrospinal fluid (CSF) [Na], mimicking the effects of a high salt diet in salt-sensitive strains and causing sympathetic hyperactivity and a pressor response that are mediated via both an endogenous brain ouabainlike substance (OLS) and the brain...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.06.056
更新日期:2004-09-24 00:00:00
abstract::Radioautography was used to study the intraneuronal distribution of [3H]-serotonin (5-HT) and/or its derivatives selectively taken up by the olfactory bulb (OB) serotonergic terminals and subsequently transported to their parent cell bodies in the midbrain raphe nuclei. This was done 24 h after injection of [3H]5-HT i...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(80)90405-9
更新日期:1980-09-08 00:00:00
abstract::Peripheral nerve fascicles are deliberately impaled during microneurography experiments. We have assessed conduction block under these circumstances. First we recorded compound action potentials (CAP) in the sural nerve following stimulation of the sciatic nerve. The insertion of injection and microneurography electro...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91538-4
更新日期:1993-12-24 00:00:00
abstract::The ability to recognize previous experience depends on two neurocognitive processes, familiarity, fast-acting and relatively automatic, and recollection, slower-acting and more effortful. Familiarity appears to mature relatively early in development and is maintained with aging, whereas recollection shows protracted ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.11.016
更新日期:2010-01-15 00:00:00
abstract::In the present investigation, electron probe X-ray microanalysis was used to characterize the effects of in vitro ouabain (2 mM) or anoxia on elemental composition (e.g. Na, K, Ca) and water content of rat peripheral (tibial) nerve myelinated axons and Schwann cells. Results showed that independent of axon size, both ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00855-k
更新日期:1995-10-02 00:00:00
abstract::Oxidative stress has been suggested to play a key role in the neuropathogenesis of HIV infection. HIV proteins (gp120, Tat) and proinflammatory cytokines can trigger the production of reactive oxygen species (ROS), resulting in DNA and RNA lesions. Among all the lesions induced by ROS, one of the most abundant lesions...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2012.04.001
更新日期:2012-06-06 00:00:00
abstract::The neuronal mechanisms of sneezing were examined in precollicular-postmammillary decerebrate cats. Mechanical stimulation (frequency 20 Hz, peak-to-peak displacement 0.5 mm) of the nasal membrane evoked a series of sneezes. In the same preparation, electrical stimulation (duration 0.2 ms, frequency 10 Hz, 5-50 microA...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90171-7
更新日期:1990-03-19 00:00:00
abstract::Considerable evidence indicates an enhanced sympathetic innervation of muscular resistance arteries in the spontaneously hypertensive rat (SHR) compared with its normotensive Wistar-Kyoto (WKY) control. Since nerve growth factor (NGF) is known to affect the growth of sympathetic nerves, we have utilized a sensitive tw...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91012-h
更新日期:1993-06-18 00:00:00
abstract::Tetanic stimulation at 50 Hz elicited long-term potentiation (LTP) in the hippocampal CA1 region of 28-day-old control animals. In contrast, no significant potentiation was seen following the same tetanus in animals chronically exposed to 1000 ppm of lead acetate during development. Gender differences were observed in...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02657-3
更新日期:2000-09-08 00:00:00
abstract::The measurements of the ion conductances in molluscan somata, published by Connor and Stevens (1971), were converted into Hodgkin-Huxley like equations. The accuracy of these equations was shown with simulations. An advantage of these equations, compared to the original equations for the fast outward K+(A)-conductance...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90120-4
更新日期:1986-09-10 00:00:00
abstract::Previous studies focusing on the relationship between lateralization of language function and age suffer from lack of a balanced distribution of age and handedness among participants, especially in the extremes of age. This limits our understanding of the influence of these factors on lateralization of language circui...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2017.08.021
更新日期:2017-11-01 00:00:00
abstract::This study examined the involvement of N-methyl-D-aspartate receptors (NMDARs) in experience-dependent synaptic plasticity. Rats chronically received an NMDA receptor antagonist (AP5) or saline (SAL) and were exposed to individual cages (IC) or environmental enrichment (EC). AP5 impaired measures of hippocampal-depend...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02379-9
更新日期:2000-07-14 00:00:00
abstract::Numerous studies of neonatal neuronal development in mammals have revealed that neuronal cell death following axotomy is apoptotic in nature. In adult animals, however, neuronal cell death following axonal injury may or may not exhibit features of apoptosis. Bcl-2 and Bax have been identified as inhibitor and promoter...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01315-3
更新日期:1999-05-08 00:00:00