Protein kinase C inhibitors enhance the synergistic mitogenic effects of ethanolamine analogues and insulin in NIH 3T3 fibroblasts.

abstract：:Incorporation of [14C]serine into phosphatidylserine by the base exchange reaction in Ehrlich ascites tumor cells is inhibited by 30% by glucose and by 60% by 2-deoxyglucose (10 mM each). The inhibition by thapsigargin (0.2 microM) amounts to 80%. This inhibition is interpreted as being due to depletion of calcium sto...

journal_title：Biochemical and biophysical research communications

authors： Rakowska M,Wojtczak L

更新日期：1995-02-06 00:00:00

abstract：:To identify the source of arachidonic acid utilized for eicosanoid production, rabbit alveolar macrophages were challenged with 1.0 microM 12-O-tetradecanoylphorbol-13-acetate (TPA) or 3.8 microM Ca+2 ionophore A23187 for 3 h. Upon stimulation with TPA, a loss of [3H]arachidonic acid from phosphatidylcholine, phosphat...

journal_title：Biochemical and biophysical research communications

authors： Cochran FR,Connor JR,Roddick VL,Waite BM

更新日期：1985-07-31 00:00:00

abstract：:EAG1 channels belong to the KCNH family of voltage gated potassium channels. They are expressed in several brain regions and increased expression is linked to certain cancer types. Recent cryo-EM structure determination finally revealed the structure of these channels in atomic detail, allowing computational investiga...

journal_title：Biochemical and biophysical research communications

authors： Bernsteiner H,Bründl M,Stary-Weinzinger A

更新日期：2017-02-26 00:00:00

abstract：:We report the cloning and characterization of both the cDNA (tufA) and genomic clones encoding for a chloroplast translation elongation factor (EF-Tu) from pea. The analysis of the deduced amino acids of the cDNA clone reveals the presence of putative transit peptide sequence and four GTP binding domains and two EF-Tu...

journal_title：Biochemical and biophysical research communications

authors： Singh BN,Mishra RN,Agarwal PK,Goswami M,Nair S,Sopory SK,Reddy MK

更新日期：2004-07-23 00:00:00

abstract：:The α2-adrenoceptor inducer dexmedetomidine protects against acute lung injury (ALI), but the mechanism of this effect is largely unknown. The present study investigated the effect of dexmedetomidine on apoptosis induced by lipopolysaccharide (LPS) and the relationship between this effect and gap junction intercellula...

journal_title：Biochemical and biophysical research communications

authors： Zhang Y,Tan X,Xue L

更新日期：2018-01-01 00:00:00

abstract：:Matrix metalloproteinases (MMP-2 and -9) play an important role in the tumor metastasis through cleavage of proinflammatory cytokines. Violacein a small molecule produced by Chromobacterium violaceum and has been implicated with anti-cancer effects. In this study we investigated the molecular basis of violacein mediat...

journal_title：Biochemical and biophysical research communications

authors： Platt D,Amara S,Mehta T,Vercuyssee K,Myles EL,Johnson T,Tiriveedhi V

更新日期：2014-12-05 00:00:00

abstract：:UCP-2 is a member of the emerging family of UCP homologues. Upon high-fat feeding, UCP-2 mRNA levels are increased in epididymal fat pads of A/J mice, suggesting that the flux of fatty acids entering adipose tissue may regulate UCP-2 gene expression. Since fatty acids act as positive transcriptional regulators of lipi...

journal_title：Biochemical and biophysical research communications

authors： Aubert J,Champigny O,Saint-Marc P,Negrel R,Collins S,Ricquier D,Ailhaud G

更新日期：1997-09-18 00:00:00

abstract：:A focal freeze injury to rat cerebral cortex induces an early (less than 5 min) increase in brain ornithine decarboxylase activity and an accumulation of polyamines involving cerebral microvessels. This polyamine synthesis correlates with the abnormal increase in microvascular permeability, monitored by uptake of Evan...

journal_title：Biochemical and biophysical research communications

authors： Koenig H,Goldstone AD,Lu CY

更新日期：1983-11-15 00:00:00

abstract：:Although the first members of the Schlafen gene family were first described almost 10 years ago, the precise molecular/biochemical functions of the proteins they encode still remain largely unknown. Roles in cell growth, haematopoietic cell differentiation, and T cell development/maturation have, with some experimenta...

journal_title：Biochemical and biophysical research communications

authors： Neumann B,Zhao L,Murphy K,Gonda TJ

更新日期：2008-05-23 00:00:00

abstract：:In our previous report we described the endocytotic incorporation of coffee bean alpha-galactosidase conjugated to human alpha-2-macroglobulin (alpha-2-M) into cultured fibroblasts derived from a patient with Fabry's disease (1). The fate of internalized alpha-galactosidase according to the method described in the abo...

journal_title：Biochemical and biophysical research communications

authors： Osada T,Kuroda Y,Ikai A

更新日期：1987-03-30 00:00:00

abstract：:Bovine thyroid 100,000 X g supernatant contained calcium-activated, calmodulin-dependent protein kinase (PK-CaM) activity. The PK-CaM was partially purified using ion-exchange chromatography and characterized. PK-CaM, using casein as exogenous substrate, was not stimulated by Ca2+(0-500 microM) or calmodulin (1-10 mic...

journal_title：Biochemical and biophysical research communications

authors： Friedman Y,Henricks L,Poleck T,Levasseur S,Burke G

更新日期：1986-10-15 00:00:00

abstract：:Lysine-specific demethylase 1 (LSD1) is upregulated in many cancers, especially neuroblastoma. We set out to explore whether geranylgeranoic acid (GGA) inhibits LSD1 activity by using recombinant human LSD1. GGA inhibited LSD1 activity with IC50 similar to that of the clinically used drug tranylcypromine. In human neu...

journal_title：Biochemical and biophysical research communications

authors： Sakane C,Okitsu T,Wada A,Sagami H,Shidoji Y

更新日期：2014-01-31 00:00:00

abstract：:Ribosome-inactivating proteins (RIPs) are N-glycosidases (EC3.2.2.22) that universally inactivate the ribosome, thereby inhibiting protein biosynthesis. In this study, a novel type I RIPs named SEBIN was identified in Sapium sebiferum. Nuclear acid depurine experiment showed that SEBIN had rRNA N-Glycosidase activity....

journal_title：Biochemical and biophysical research communications

authors： Wu Y,Mao Y,Jin S,Hou J,Du H,Yang M,Wu L

更新日期：2015-08-07 00:00:00

abstract：:Some inroads have been made into characterizing histone variants and post translational modifications of histones in Trypanosoma brucei. Histone variant H2BV lysine 129 is homologous to Saccharomyces cerevisiae H2B lysine 123, whose ubiquitination is required for methylation of H3 lysines 4 and 79. We show that T. bru...

journal_title：Biochemical and biophysical research communications

authors： Mandava V,Janzen CJ,Cross GA

更新日期：2008-04-18 00:00:00

abstract：:Intracellular symbiotic relationships are prevalent between cnidarians, such as corals and sea anemones, and the photosynthetic dinoflagellate symbionts. However, there is little understanding about how the genes express when the symbiotic relationship is set up. To characterize genes involved in this association, the...

journal_title：Biochemical and biophysical research communications

authors： Kuo J,Chen MC,Lin CH,Fang LS

更新日期：2004-05-21 00:00:00

abstract：:The recognition and removal of interstrand cross-links is perhaps the least understood of all repair pathways in eukaryotic cells. We have shown previously that uncoupling of cross-links occurs in mammalian cell extracts and have identified a number of factors that mediate this process. However, we have not observed c...

journal_title：Biochemical and biophysical research communications

authors： Lu X,Zhang N,Vasquez K,Barton M,Legerski R

更新日期：2005-10-14 00:00:00

abstract：:Branched-chain alpha-keto acid dehydrogenase (BCKDH) kinase (BDK) is responsible for the regulation of BCKDH complex, which is the rate-limiting enzyme in the catabolism of branched-chain amino acids (BCAAs). In the present study, we investigated the expression and activity of hepatic BDK in spontaneous type 2 diabete...

journal_title：Biochemical and biophysical research communications

authors： Doisaki M,Katano Y,Nakano I,Hirooka Y,Itoh A,Ishigami M,Hayashi K,Goto H,Fujita Y,Kadota Y,Kitaura Y,Bajotto G,Kazama S,Tamura T,Tamura N,Feng GG,Ishikawa N,Shimomura Y

更新日期：2010-03-05 00:00:00

abstract：:Rat type 1 angiotensin II receptor has two subtypes, namely type 1a and type 1b. The regulation of the expressions of these two subtype receptor mRNAs was studied by using a competitive polymerase chain reaction method. The expression of the type 1a mRNA in the liver was negatively and that of the type 1b mRNA in the ...

journal_title：Biochemical and biophysical research communications

authors： Iwai N,Inagami T,Ohmichi N,Nakamura Y,Saeki Y,Kinoshita M

更新日期：1992-10-15 00:00:00

abstract：:We have cloned two cDNA isoforms as well as genomic sequences of the mouse Prx V gene and characterized their molecular genetic features. Two isoforms of the mouse Prx V cDNA were identified from liver and testis. The testis-originated long transcripts had extra 1164-bp 5'-UTR sequences compared to the liver-originate...

journal_title：Biochemical and biophysical research communications

authors： Lee TH,Kim SJ,Kang SW,Lee KK,Rhee SG,Yu DY

更新日期：2000-04-13 00:00:00

abstract：:Using PAGE--Autoradioblotting technique we have characterized an E--FABP in human epidermal cells that is distinct from liver-, heart-, intestine- and adipose tissue-FABPs. FABP radiobinding analysis was performed directly on protein extracts without prior partial purification. E-FABP has a Mr of approximately 15 kDa ...

journal_title：Biochemical and biophysical research communications

authors： Siegenthaler G,Hotz R,Chatellard-Gruaz D,Jaconi S,Saurat JH

更新日期：1993-01-29 00:00:00

abstract：:An 18-kDa lectin, with an N-terminal sequence displaying slight similarity to some lectins and fungal immunomodulatory proteins, was isolated from the mushroom Ganoderma capense (Lloyd) Teng. It exhibited more potent mitogenic activity than that of concanavalin A toward mouse splenocytes, and antiproliferative activit...

journal_title：Biochemical and biophysical research communications

authors： Ngai PH,Ng TB

更新日期：2004-02-20 00:00:00

abstract：:We reported previously that GRF stimulates release of neurotensin (NT) by a clonal line of rat C cells (44-2 C). We report here that GRF stimulates calcitonin (CT) and cAMP release in these cells. For release experiments, replicate cultures are incubated for 5-180 min in serum-free defined medium. CT, NT and cAMP are ...

journal_title：Biochemical and biophysical research communications

authors： Zeytin F,Brazeau P

更新日期：1984-09-17 00:00:00

abstract：:Amyloid-beta (Abeta) is the core component of senile plaques, which are the pathological markers for Alzheimer's disease and cerebral amyloid angiopathy. DNA methylation/demethylation plays a crucial role in gene regulation and could also be responsible for presentation of senescence. Oxidative stress, which may be in...

journal_title：Biochemical and biophysical research communications

authors： Chen KL,Wang SS,Yang YY,Yuan RY,Chen RM,Hu CJ

更新日期：2009-01-02 00:00:00

abstract：:The development of central nervous system (CNS) neuronal networks involves processes including neuroblast migration, axonal pathfinding, and synaptogenesis. To evaluate the role of the axonal guidance molecule DSCAM in CNS connectivity, we generated a lacZ reporter construct, Pr1.8-betagal, containing a 1.8kb fragment...

journal_title：Biochemical and biophysical research communications

authors： Barlow GM,Lyons GE,Richardson JA,Sarnat HB,Korenberg JR

更新日期：2002-11-22 00:00:00

abstract：:Endothelin-1 (ET-1) contracted the rabbit tracheal smooth muscle (RTSM), yielding a bell-shaped tension-concentration curve. Moreover, ET-1 induced concentration- and time-dependent increases in cAMP concentrations in RTSM (EC(50), 58 nM; t(1/2), 2.4 min). Pretreatment with the AC inhibitors, SQ-22536, or 2'-5'-dideox...

journal_title：Biochemical and biophysical research communications

authors： El-Mowafy AM,Biggs DF

更新日期：2001-08-17 00:00:00

abstract：:Intracellular transport and storage of retinoids were shown to be conducted by fatty acid-binding protein (FABP). When rat liver cytosol was gel filtrated, retinyl palmitate-binding activity was mainly eluted in the fraction with a Mr. of around 14,000, in which both FABP and cellular retinol-binding protein (CRBP) co...

journal_title：Biochemical and biophysical research communications

authors： Fukai F,Kase T,Shidotani T,Nagai T,Katayama T

更新日期：1987-09-30 00:00:00

abstract：:We have identified a genomic DNA fragment, using the PCR method with degenerate oligonucleotide primers which contain the conserved sequence of the RING finger domain. Using the DNA fragment as a probe, a novel cDNA was cloned from human and mouse testis. The cDNA had a domain structure of the typical RING-B box-coile...

journal_title：Biochemical and biophysical research communications

authors： Orimo A,Yamagishi T,Tominaga N,Yamauchi Y,Hishinuma T,Okada K,Suzuki M,Sato M,Nogi Y,Suzuki H,Inoue S,Yoshimura K,Shimizu Y,Muramatsu M

更新日期：2000-09-16 00:00:00

abstract：:NF-kappaB plays an important role in oncogenesis. Recently, we have demonstrated that loss of p53 function enhances DNA binding and transcriptional activities of NF-kappaB via IKKalpha and IKKbeta, and that glycolysis, activated by NF-kappaB, has an integral role in oncogene-induced cell transformation. Here, we show ...

journal_title：Biochemical and biophysical research communications

authors： Kawauchi K,Araki K,Tobiume K,Tanaka N

更新日期：2008-07-18 00:00:00

abstract：:During the search for ANP receptor ligands of microbial origin, we isolated a novel polysaccharide, HS-142-1, from culture broth of Aureobasidium sp. HS-142-1 inhibited [125I]-rANP binding to ANP receptor in rabbit kidney cortex membranes with an IC50 of 0.3 mu g/ml, but gave no effects on specific binding of [125I]-E...

journal_title：Biochemical and biophysical research communications

authors： Morishita Y,Sano T,Ando K,Saitoh Y,Kase H,Yamada K,Matsuda Y

更新日期：1991-05-15 00:00:00

abstract：:Specific polyclonal antibodies raised against purified human platelet Ca2+-ATPase were used with protein A-gold immunocytochemistry to localize this protein in human platelets. Immunolabeling specifically detected Ca2+-ATPase over the surface connected membrane system (SCS) in sections of paraformaldehyde-fixed, Lowic...

journal_title：Biochemical and biophysical research communications

authors： Herbener GH,Dean WL

更新日期：1988-06-16 00:00:00