Cellular component of vascular calcification. Fibroblasts are essential for calcium deposition in cultured cells.

Abstract:

:It has been reported that calcium deposition and calcium content in cultured human aortic smooth muscle cells (SMC) increased when the cells are incubated in a medium with a high phosphate concentration (2 mM). To determine the cellular components or soluble factors contributing to the deposition, we cultured commercially available SMC, fibroblasts (Fb) and endothelial cells (Ed). These cells and their mixtures were incubated for 10 days in normal or high-phosphate media. Calcium crystals were stained by the von-Kossa staining and counted in the defined area. Calcium content was measured by a colorimetric assay. SMC were incubated in high-phosphate media (up to 2 mM) or beta-glycerophosphate (beta-GP) media, resulting in no obvious deposition of calcium crystals, irrespective of the coating of type I collagen on the dish. Next, various combinations of cells were cultured, and a significant number of depositions were observed only when Fb were included in the combination. The calcium content was significantly higher in cultures of SMC and Fb. The calcium deposition on single or mixture of the cells did not increase compared with control when cells were incubated in a high concentration of phosphate, cultured in the existence of beta-GP or uremic serum. We therefore conclude that Fb, rather than SMC or Ed, are essential for calcium deposition and calcium accumulation in culture. Phosphate concentration in the medium and uremic serum did not influence the deposition of calcium.

journal_name

Nephron

journal_title

Nephron

authors

Watanabe Y,Suzuki M,Oyama Y,Kusano E,Tamba K,Iimura O,Ito C,Imai M,Asano Y

doi

10.1159/000065461

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

840-5

issue

4

eissn

1660-8151

issn

2235-3186

pii

65461

journal_volume

92

pub_type

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