Regional characterization of G-protein subunits in glomeruli, cortices and medullas of the rat kidney.

Abstract:

:We examined the types of guanine nucleotide-binding regulatory (G) protein subunits in isolated glomeruli, cortices excluding glomeruli and medullas of rat kidneys using bacterial toxin-catalyzed adenosine 5'-diphosphate (ADP) ribosylation and specific immunoblots. ADP ribosylation catalyzed by cholera or pertussis toxin revealed the presence of stimulatory G (Gs) or inhibitory G (Gi) proteins in membranes of the 3 segments of the kidney. Immunoblots further demonstrated the existence of several G-protein subunits, two Gs-protein alpha-subunits (G alpha s: 45 and 52 kD), Gi-protein alpha 1, alpha 2 and alpha 3-subunits (G alpha i1, G alpha i2: 40-41 kD, G alpha i3: 40 kD), bacterial toxin-insensitive G-protein alpha q- and alpha 11-subunits (G alpha q/11: 42 kD) and G-protein beta-subunits (G beta: 35-36 kD), in membranes of the preparations. The predominant subspecies of G alpha s was a 52-kD protein in glomerular membranes and a 45-kD protein in membranes of cortices and medullas. All of the G-protein subunits examined, however, were not detected in cytosolic fractions of glomeruli, cortices and medullas. Thus, we conclude that detectable quantities of several G-protein subunits including the new G-protein subunit, G alpha q/11, are present in membranes of glomeruli, cortices not containing glomeruli and medullas from the rat kidney. Both the existence of G alpha i1 and/or G alpha i2 subunits in glomeruli and the presence of G alpha q/11 subunits in the 3 preparations are new evidence.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Nephron

journal_title

Nephron

authors

Yanagisawa H,Kurihara N,Klahr S,Morrissey J,Wada O

doi

10.1159/000187862

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

447-52

issue

4

eissn

1660-8151

issn

2235-3186

journal_volume

66

pub_type

杂志文章

相关文献

NEPHRON文献大全
  • Exercise capacity in hemodialysis, CAPD, and renal transplant patients.

    abstract::Eighteen hemodialysis, 12 chronic ambulatory peritoneal dialysis (CAPD), and 20 renal transplant patients performed maximal treadmill exercise tests. Heart rates and blood pressures were determined every minute and maximal oxygen consumption was measured directly. Exercise capacity as measured by VO2 max is low in dia...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000183632

    authors: Painter P,Messer-Rehak D,Hanson P,Zimmerman SW,Glass NR

    更新日期:1986-01-01 00:00:00

  • Significance of platelet-derived microparticles and activated platelets in diabetic nephropathy.

    abstract::We measured levels of platelet-derived microparticles (PMP), which have coagulative activity and are produced by platelet activation or physical stimulation, and CD62P/CD63-positive platelets in patients with diabetes mellitus to determine their clinical significance and effects on complications of diabetes including ...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000045292

    authors: Omoto S,Nomura S,Shouzu A,Hayakawa T,Shimizu H,Miyake Y,Yonemoto T,Nishikawa M,Fukuhara S,Inada M

    更新日期:1999-01-01 00:00:00

  • Nonpharmacological, Biomechanical Approaches to Control Inflammation in Acute Kidney Injury.

    abstract::Inflammation is broadly recognized as an important factor in the pathogenesis of acute kidney injury (AKI), but pharmacological approaches to alleviate inflammation in AKI have been without success in clinical trials. Neuromodulation by nonpharmacological methods is emerging as a novel therapeutic strategy to treat in...

    journal_title:Nephron

    pub_type: 杂志文章,评审

    doi:10.1159/000477218

    authors: Tanaka S,Inoue T,Hossack JA,Okusa MD

    更新日期:2017-01-01 00:00:00

  • Effect of experimental chronic renal failure upon the synthesis of thyroid hormones in rats.

    abstract::The authors utilized two groups of adult male rats to study the effect of chronic-uremic toxemia on the synthesis of thyroid hormones. The control group was sham operated, while the other group had about 70--80% of the left kidney tissue surgically excised and a total right nephrectomy performed 10 days later. At the ...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000181516

    authors: Costa e Silva A,Albuquerque RH,Santana JP

    更新日期:1978-01-01 00:00:00

  • Prevalence of hypocitraturia and hypopyrophosphaturia in recurrent calcium stone formers: as isolated defects or associated with other metabolic abnormalities.

    abstract::Several underlying metabolic abnormalities may be present in patients with recurrent calcium calculus disease (RCCD). The aim of this study was to determine the prevalence of deficiencies of 2 well-known potent inhibitors of crystal formation and growth, citrate and pyrophosphate, in the various metabolic subgroups an...

    journal_title:Nephron

    pub_type: 临床试验,杂志文章

    doi:10.1159/000186179

    authors: Laminski NA,Meyers AM,Sonnekus MI,Smyth AE

    更新日期:1990-01-01 00:00:00

  • Recovery of decreased ability of peripheral-blood mononuclear cells from chronic renal failure to produce interleukin-1 alpha and beta after renal transplantation.

    abstract::The ability of cultured peripheral-blood mononuclear cells (PBMC) to release interleukin-1 alpha and beta (IL-1 alpha, IL-1 beta) in response to concanavalin A (con A) was investigated in patients with chronic renal failure (CRF) and in renal transplant recipients. Mean IL-1 alpha level released by PBMC of healthy sub...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000186478

    authors: Kang XX,Hirano T,Oka K,Tamaki T,Sakurai E,Kaji N,Yoshida M,Kozaki M

    更新日期:1991-01-01 00:00:00

  • Effects of dietary protein on uranyl-nitrate-induced acute renal failure.

    abstract::The effects of dietary protein both before and after uranyl-nitrate-induced acute renal failure were investigated. Male Sprague-Dawley rats were maintained on high-protein (60%), normal-protein (20%), low-protein (5%) and no-protein diets for 8 days prior to intravenous administration of uranyl nitrate (10 mg/kg). Imm...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000184167

    authors: Andrews PM,Bates SB

    更新日期:1987-01-01 00:00:00

  • Renin release and the refractoriness to acute renal failure of rats recovering from prior renal failure.

    abstract::Renin substrate concentrations and the release of renin in response to the rapid removal of 15 ml/kg weight blood or subcutaneous administration of 500 microgram/kg body weight isoproterenol were measured in control rats and animals recovering from prior myohemoglobinuric acute renal failure (recovery rats). Blood for...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000181429

    authors: Carvalho JS,Landwehr DM,Oken DE

    更新日期:1978-01-01 00:00:00

  • Parathyroid hormone effect on the fragility of human young and old red blood cells in uremia.

    abstract::Parathyroid hormone (PTH) is elevated in patients with chronic renal failure (CRF) and was suggested to be one of the factors responsible for the anemic syndrome of these patients because it raises the osmotic fragility of the red blood cells (RBC). In the present study, the youngest and oldest RBC were separated from...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000183633

    authors: Malachi T,Bogin E,Gafter U,Levi J

    更新日期:1986-01-01 00:00:00

  • Glomerular-tubular balance for bicarbonate in man.

    abstract::We recently showed in dogs that there is a close relationship between bicarbonate reabsorption and glomerular filtration rate (GFR). The present studies were designed to confirm these findings in five normal men. Bicarbonate titrations were done after ingesting a low protein diet for one week and were repeated followi...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000180453

    authors: Bennett CM,Falkinburg NR,Springberg PD

    更新日期:1975-01-01 00:00:00

  • Human immunodeficiency virus-associated Kaposi's sarcoma in a pediatric renal transplant recipient.

    abstract::An 11-year-old boy developed Kaposi's sarcoma and progressive T lymphocyte deficiency 5 years after cadaveric kidney transplantation for end-stage renal disease. He had received 17 individual red blood cell transfusions prior to and during transplantation in 1980. Human immunodeficiency virus (HIV) was cultured from b...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000184458

    authors: Malekzadeh MH,Church JA,Siegel SE,Mitchell WG,Opas L,Lieberman E

    更新日期:1987-01-01 00:00:00

  • Effect of levamisole on skin reactions to DNCB in chronically dialyzed patients.

    abstract::The local cutaneous inflammatory response to DMCB sensitization was positive in only 35% of 20 uremic patients, and the delayed skin reaction was positive in only 15%. The reaction to DMCB challenge was markedly delayed in some patients. Improved response to DNCB re-challenge followed levamisole administration in only...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000181858

    authors: Modai D,Sagi DB,Maor J

    更新日期:1980-01-01 00:00:00

  • Low dose ciclosporin from the early postoperative period yields potent immunosuppression after renal transplantation.

    abstract::This study sought to determine if low doses of ciclosporin (CS) designed to give fasting serum levels of 50-100 ng/ml achieve effective immunosuppression when used from the early postoperative period after renal transplantation. Ninety-four primary renal transplant recipients were studied. Group 1 patients were treate...

    journal_title:Nephron

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000186007

    authors: Brady HR,Kamel KS,Harding ME,Cook GT,deVeber GA,Cardella CJ

    更新日期:1990-01-01 00:00:00

  • C-reactive protein and interleukin-6 levels are related to renal function in predialytic chronic renal failure.

    abstract:BACKGROUND:Several studies have provided convincing evidence that in apparently healthy subjects elevated serum levels of plasma C-reactive protein (CRP) are associated with an increased risk of experiencing myocardial infarction and sudden cardiac death. It has been claimed that, in dialytic patients, the hepatic synt...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000065018

    authors: Panichi V,Migliori M,De Pietro S,Taccola D,Bianchi AM,Giovannini L,Norpoth M,Metelli MR,Cristofani R,Bertelli AA,Sbragia G,Tetta C,Palla R,Colombo R

    更新日期:2002-08-01 00:00:00

  • Carbamazepine-induced hyponatremia in a patient with partial central diabetes insipidus.

    abstract::A 51-year-old Japanese man was referred for the evaluation of persistent hyponatremia. The serum sodium level remained around 120 mmol/l despite mild water restriction. His past history included chronic alcoholism, myocardial infarction and lumbar disc herniation. Carbamazepine (200 mg, b.i.d.) has been used for more ...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000187295

    authors: Kamiyama T,Iseki K,Kawazoe N,Takishita S,Fukiyama K

    更新日期:1993-01-01 00:00:00

  • Serum leptin concentrations in patients on hemodialysis.

    abstract::Serum leptin concentrations in normal humans have been reported to correlate with the body mass index (BMI) as well as with the body fat mass. In this study, we measured serum leptin concentrations in 107 patients on hemodialysis, 30 of whom had diabetes mellitus as the cause, and examined the clinical significance. F...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000045122

    authors: Nakazono H,Nagake Y,Ichikawa H,Makino H

    更新日期:1998-09-01 00:00:00

  • Technique for the performance of repeatable renal clearances in the conscious male dog.

    abstract::A preparation for performance of renal clearances in the conscious dog is described. The important aspect of the technique is the use of a Pavlov sling and a specially designed restraining apparatus. Of most importance is the use of a 7 French Swan-Ganz balloon-tipped catheter for urinary bladder catheterization. The ...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000180595

    authors: Garner D,Laks MM

    更新日期:1976-01-01 00:00:00

  • Effects of renal nerves on renal hemodynamics. I. Direct stimulation and carotid occlusion.

    abstract::Renal nerves were stimulated either directly (loop electrodes) or indirectly (bilateral carotid occlusion with maintenance of control blood pressure) in anesthetized dogs to determine the effects on renal blood flow (RBF), glomerular filtration rate (GFR), sodium excretion (UNaV), and intrarenal distribution of blood ...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000180454

    authors: Katz MA,Shear L

    更新日期:1975-01-01 00:00:00

  • Nephropathy and mixed cryoglobulinemia: evidence for an immune complex pathogenesis.

    abstract::Mixed IgM-IgG cryoglobulins were present in the serum of a patient with the nephrotic syndrome, recurrent purpura, chronic active hepatitis and ascites. A renal biopsy demonstrated deposition of IgM, IgG, and complement components C3 and C4 in intracapillary 'coagula' and glomerular capillary walls. No glomerular depo...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000180591

    authors: Zimmerman SW,Dreher WH,Burkholder PM,Goldfarb S,Weinstein AB

    更新日期:1976-01-01 00:00:00

  • Exercise and Prediabetes after Renal Transplantation (EXPRED): Protocol Description.

    abstract:BACKGROUND:Post-transplant diabetes mellitus (PTDM) is a frequent and severe complication after renal transplantation. In fact, PTDM is a risk factor for both infection and cardiovascular diseases. The prevalence and incidence of PTDM have a bimodal evolution: early (up to 3 months) and late PTDM (beyond 12 months). Th...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000511320

    authors: Morales Febles R,Negrín Mena N,Rodríguez-Rodríguez AE,Díaz Martín L,González Rinne F,Marrero Miranda D,González Rinne A,Álvarez González A,Pérez Tamajón L,Acosta Sørensen C,Rodríguez Hernández A,Domínguez-Rodríguez A,García Baute

    更新日期:2021-01-01 00:00:00

  • Effects of rhubarb tannins on uremic toxins.

    abstract::The effects of each of several tannins purified from Rhei Rhizoma on serum constituents were investigated in rats with adenine-induced renal failure. Blood levels of urea nitrogen, methylguanidine (MG), and guanidinosuccinic acid (GSA) were significantly decreased in rats given (-)-epicatechin 3-O-gallate at a dose of...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000186406

    authors: Yokozawa T,Fujioka K,Oura H,Nonaka G,Nishioka I

    更新日期:1991-01-01 00:00:00

  • Suppression of parathyroid hormone by therapy with a mixture of keto analogues/amino acids in hemodialysis patients.

    abstract::In the present study the influence of a mixture of keto analogues/amino acids (KA) on PTH levels in 18 RDT patients was examined. Ten of them were treated with KA over a period of 3 months, while the other 8 patients served as control group. The KA-treated patients showed a significant fall in PTH and plasma phosphate...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000183804

    authors: Lindenau K,Kokot F,Fröhling PT

    更新日期:1986-01-01 00:00:00

  • Systemic factors and renal hemodynamic effects of high-protein meal versus low-protein meal in conscious dogs.

    abstract::The effects of a high-protein (HP) and low-protein (LP) meal on glomerular filtration rate (GFR), effective renal plasma flow (ERPF), and renal vascular resistance (RVR) were examined in conscious dogs. Ingestion of the HP and LP meal produced a temporary increase in systemic hemodynamic parameters due in part to a 'c...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000188228

    authors: Zawada ET,Saelens DA,Alavi FK,Lembke JM

    更新日期:1994-01-01 00:00:00

  • Effect of fructose and mannitol on dog renal cortical adenine nucleotide content, in vivo.

    abstract::The renal cortex of anesthetized dogs was found to contain per gram lyophilized dry weight 9.5+/-0.6 micronmol ATP, 3.2+/-0.5 MICRONMOL ADP, 0.68+/-0.3 MICRONMOL AMP, and 22.5+/-4.4 micronmol Pi. These amounts were unchanged by the administration of moderate amounts of saline or hypertonic mannitol in load doses of ab...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000180833

    authors: Urbaitis BK,Blake WD

    更新日期:1977-01-01 00:00:00

  • Platelet count and thrombopoietic activity in patients with chronic renal failure.

    abstract::The frequency of thrombocytopenia in patients with chronic renal failure (CRF) is controversial. This study was undertaken to investigate the platelet count in 55 patients with end-stage renal disease on maintenance hemodialysis and in 19 patients with CRF before hemodialysis had begun. In both groups platelet counts ...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000184118

    authors: Gafter U,Bessler H,Malachi T,Zevin D,Djaldetti M,Levi J

    更新日期:1987-01-01 00:00:00

  • Serial studies of the renin system in rats with glycerol-induced renal failure.

    abstract::Serial changes in plasma of renin concentration (PRC), renin-substrate concentration (PSC) and renin activity (PRA) were followed in rats during glycerol-induced acute renal failure. There was an early but transient increase in PRC and a more delayed and prolonged rise in PSC. PRA began to rise a few hours after glyce...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000181196

    authors: Carvalho JS,Page LB

    更新日期:1978-01-01 00:00:00

  • Effect of sodium bicarbonate preloading on ischemic renal failure.

    abstract::Rats pretreated with sodium bicarbonate were functionally protected from the damage of bilateral renal artery occlusion. The rise in serum creatinine (day 1 minus day 0) during the first 24 h after ischemia was 2.88 +/- 0.28 mg% in the bicarbonate-loaded animals versus 3.90 +/- 0.26 mg% in their matched controls (p le...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000183915

    authors: Atkins JL

    更新日期:1986-01-01 00:00:00

  • Clinicopathological study of patients with mesangial isolated C3d deposition in various glomerular diseases.

    abstract::The clinicopathological findings of isolated mesangial C3d deposition in the absence of other complement components or immunoglobulins are summarized. 55 out of 242 individual human renal biopsies examined by immunoperoxidase microscopy had isolated C3d deposition. This group consisted of 12 patients with chronic glom...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000184339

    authors: Doi T,Kanatsu K,Suehiro F,Nagai H,Yoshida H,Hamashima Y

    更新日期:1987-01-01 00:00:00

  • Urinary Kidney Injury Molecule-1 but Not Urinary Neutrophil Gelatinase Associated Lipocalin Is Increased after Short Maximal Exercise.

    abstract:BACKGROUND AND AIMS:Urinary neutrophil gelatinase associated lipocalin (uNGAL) and urinary kidney injury molecule-1 (uKIM-1) are markers of acute kidney injury. The albuminuria is a well-known abnormality after physical exercise. The aim of this study was to investigate changes in uNGAL and uKIM-1 after intensive exerc...

    journal_title:Nephron

    pub_type: 杂志文章

    doi:10.1159/000481179

    authors: Wołyniec W,Ratkowski W,Urbański R,Bartoszewicz M,Siluk D,Wołyniec Z,Kasprowicz K,Zorena K,Renke M

    更新日期:2018-01-01 00:00:00

  • Renal accumulation of glycosphingolipids. Report of a case and a review of literature.

    abstract::A 62-year-old woman gave clinical manifestation of liver cirrhosis. Urinary protein was false positive, no uremia was found and renal changes were entirely overlooked. Deposition of abundant lipids (globoside and ceramide trihexoside) was found in the kidneys; essentially degenerative changes of the tubular epithelia ...

    journal_title:Nephron

    pub_type: 杂志文章,评审

    doi:10.1159/000180480

    authors: Miyasaki K

    更新日期:1975-01-01 00:00:00