Abstract：
:Fos protein heterodimerizes through one surface of an alpha-helical domain called the leucine zipper. We have investigated the effect of destabilizing this domain by multiply substituting small residues of its non-interacting surface with glycine. Ternary complex formation between mutated Fos, Jun and DNA was determined in vitro in the presence of denaturant. We also tested the ability of constitutively expressed, mutated Fos proteins to support anchorage independent growth of the cell line Rat1A. Combinations of two substitutions are tolerated in both assays of Fos function, while four substitutions resulted in attenuation in both functions. Rat1A expressing one of the quadruple mutants also showed temperature sensitivity in anchorage independent growth. In dense monolayers of these cells, stromelysin (a Fos-responsive gene product) decreased in abundance as a function of temperature and was less abundant even at 34 degrees C than in cells that overexpressed the wild-type c-fos mRNA. However the mutant transgene itself appeared to show temperature sensitive expression. We suggest that creating a range of glycine substitutions for small residues in the non-interacting face of a leucine zipper might be of general use as a strategy to produce attenuated mutants of other transcription factors.
journal_name
Oncogenejournal_title
Oncogeneauthors
Nicklin MJ,Zechner D,Kislyakova Tsubject
Has Abstractpub_date
1993-07-01 00:00:00pages
1737-49issue
7eissn
0950-9232issn
1476-5594journal_volume
8pub_type
杂志文章相关文献
ONCOGENE文献大全abstract：:We reconstituted a three-dimensional gastric carcinoma model similar to invasive gastric carcinoma tissue. This model consists of a human gastric carcinoma cell line, GCTM-1, a human fibroblast cell line, TIG-1-20, and transforming growth factor-beta (TGF-beta)-containing type I collagen gel. Using this model, we were...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1207046
更新日期：2003-10-30 00:00:00
abstract：:Squamous cell carcinoma (SCC) of the skin represents a group of neoplasms which is associated with exposure to UV light. Recently, we obtained data suggesting that invasive skin cancer and its precursors derive from one original neoplastic clone. Here, the analysis were extended by loss of heterozygosity (LOH) analysi...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1202080
更新日期：1998-10-08 00:00:00
abstract：:In order to assess the functional contribution of the human c-myc promoter region in the expression of the c-myc gene, transgenic mouse lines containing a bacterial lac Z gene encoding beta-galactosidase under the control of the human c-myc protooncogene promoter were generated. Transgenic mouse embryos heterozygous f...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1995-06-15 00:00:00
abstract：:Membrane overexpression of the receptor tyrosine kinase ErbB-2 (MErbB-2) accounts for a clinically aggressive breast cancer (BC) subtype (ErbB-2-positive) with increased incidence of metastases. We and others demonstrated that nuclear ErbB-2 (NErbB-2) also plays a key role in BC and is a poor prognostic factor in ErbB...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2015.281
更新日期：2016-04-28 00:00:00
abstract：:Nuclear Foxc2 is a transcriptional regulator of mesenchymal transformation during developmental epithelial-mesenchymal transition (EMT) and has been associated with EMT in malignant epithelia. Our laboratory has shown that in normal epithelial cells Foxc2 is maintained in the cytoplasm where it promotes an epithelial ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2014.395
更新日期：2015-09-03 00:00:00
abstract：:Germline mutations in the tumor suppressor BRCA1 predispose women to breast and ovarian cancers. Current evidence demonstrates that mutations in BRCA1 do not directly result in tumor formation, but instead cause genetic instability, subjecting cells to high risks of malignant transformation. In an animal model in whic...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/sj.onc.1203269
更新日期：2000-02-21 00:00:00
abstract：:E2F is a complex family of transcription factors which appears to regulate the transcription of genes required for the S phase of the mammalian cell cycle. In the present work, we have examined the mechanisms regulating E2F-3 accumulation in mouse fibroblasts. We have determined that E2F-3 DNA binding activity is rest...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1201633
更新日期：1998-03-12 00:00:00
abstract：:A selective switch from expression of Shc1 gene to Shc3 occurs with maturation of neuronal precursors into postmitotic neurons. Previous studies showed that in the embryo, Shc1 is maximally expressed in dividing CNS stem cells while it is silenced in mature neurons, where it is replaced by Shc3. Under normal condition...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1208708
更新日期：2005-08-04 00:00:00
abstract：:The cellular and molecular mechanisms of tumor progression following chemotherapy are largely unknown. Here, we demonstrate that cisplatin (CDDP) treatment upregulates VEGF and Flt1 expression leading to the survival and expansion of a highly tumorigenic fraction of side-population (SP) cells in osteosarcoma (HOS), ne...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2008.38
更新日期：2008-06-26 00:00:00
abstract：:A search for protein tyrosine kinases expressed during murine cardiogenesis resulted in the isolation of a novel tyrosine kinase, designated tek, which maps to mouse chromosome 4 between the brown and pmv-23 loci. The deduced amino acid sequence of tek predicts that it encodes a putative receptor tyrosine kinase that ...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:The Wilms' Tumor 1 gene (WT1) encodes a transcription factor of the zinc-finger family. As a result of alternative RNA splicing, the gene can be expressed as four polypeptides which differ in the presence or absence of two stretches of amino acids: one of 17 residues (17aa) just N-terminal of the four zinc-fingers and...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:Adenovirus contains two nuclear oncogenes, the EIA and EIB genes, which cooperatively can transform cells through mechanisms that are not understood. The transcriptional activities of the E1A gene (transactivation and repression) are well studied. Using transient expression assays, we show here that the 19,000-Da E1B ...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Ovarian cancer is the leading cause of death among all gynecological malignancies due to the development of acquired chemoresistance and disease relapse. Although the role of cancer stem cells (CSCs), a subset of tumor cells with the self-renewal and differentiation capabilities, in therapeutic resistance is beginning...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2016.320
更新日期：2017-03-01 00:00:00
abstract：:The proangiogenic cytokine Interleukin-3 (IL-3) is released by inflammatory cells in breast and ovarian cancer tissue microenvironments and also acts as an autocrine factor for human breast and kidney tumor-derived endothelial cells (TECs). We have previously shown that IL-3-treated endothelial cells (ECs) release ext...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/s41388-017-0034-x
更新日期：2018-03-01 00:00:00
abstract：:The progression and negative outcome of a variety of human carcinomas are intimately associated with aberrant activity of the c-Met oncogene. The underlying cause of this dysregulation, however, remains a subject of discussion, as the majority of cancer patients do not present with activating mutations in c-Met recept...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2010.586
更新日期：2011-04-28 00:00:00
abstract：:To investigate the relationship between the local configuration of a gene and its level of expression, we constructed a rat cell line, Hy5, carrying a mutant polyomavirus middle T oncogene (pmt) whose overexpression converted the cells to the transformed state. The structure of the transgene was such that pmt was able...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:Cancer development results from deregulated control of stem cell populations and alterations in their surrounding environment. Notch signaling is an important form of direct cell-cell communication involved in cell fate determination, stem cell potential and lineage commitment. The biological function of this pathway ...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/onc.2008.225
更新日期：2008-09-01 00:00:00
abstract：:Tumors consistently mimic wound-generating chronic inflammation; however, why they do not heal like wounds with fibrotic scars remains unknown. The components of the tumor microenvironment, such as transforming growth factor β (TGF-β) and fibroblast growth factors (FGFs), may account for this phenomenon. Tumor formati...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2016.512
更新日期：2017-07-06 00:00:00
abstract：:The human papovaviruses BK virus (BKV) and JC virus (JCV) both encode a large-tumor (T) antigen which are highly homologous to each other as well as to simian virus 40 (SV40) large-T antigen. This high conservation of amino acid sequence has resulted in overlapping antigenicity such that immunological differentiation ...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
abstract：:Proliferating cells need to produce a large amount of energy and, at the same time, need to maintain a constant supply of biosynthetic precursors of macromolecules that are used as building blocks for generating new cells. Indeed, many cancer cells undergo a switch from mitochondrial to glycolytic metabolism and displ...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/onc.2012.529
更新日期：2013-07-25 00:00:00
abstract：:The maintenance of genome stability is essential for the cell as the integrity of genomic information guaranties reproduction of a whole organism. DNA damage occurring in response to different natural and nonnatural stimuli (errors in DNA replication, UV radiation, chemical agents, etc.) is normally detected by specia...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：10.1038/s41388-019-0980-6
更新日期：2020-01-01 00:00:00
abstract：:To explore the mechanism of estrogen-induced growth of normal endometrium, the transactivation system of the cyclin D1 gene was analysed using cultured normal endometrial glandular cells. Estradiol (E2) treatment of cultured normal endometrial glandular cells induced upregulation of c-Jun, and then cyclin D1 proteins,...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1207849
更新日期：2004-11-11 00:00:00
abstract：:The Neuregulins and their receptors, the ErbB/HER subfamily of receptor tyrosine kinases, have critical roles in animal physiology, and their deregulation is frequent in cancer. Here we report the identification of the guanine nucleotide exchange factor, phosphatidylinositol 3,4,5-triphosphate-dependent Rac exchanger ...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2010.489
更新日期：2011-03-03 00:00:00
abstract：:Each step in the synthesis of functional transcript by RNA polymerase II provides a level at which gene expression can be regulated. Control over the elongation phase of transcription is a recognized regulatory mechanism in prokaryotes; however, only recently have examples of conditional transcription elongation block...
journal_title：Oncogene
pub_type： 杂志文章,评审
doi：
更新日期：1990-06-01 00:00:00
abstract：:Many of the current antitumor therapeutic strategies are based on the perturbation of the cell cycle, especially during mitosis. Antimitotic drugs trigger mitotic checkpoint activation, mitotic arrest and eventually cell death. However, mitotic slippage represents a major mechanism of resistance to these treatments. I...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2013.601
更新日期：2015-01-29 00:00:00
abstract：:The management of pain and morbidity due to the spreading and growth of cancer within bone remains to be a paramount problem in clinical care. Cancer cells actively transform bone, however, the molecular requirements and mechanisms of this process remain unclear. This study shows that functional modulation of the alph...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1210429
更新日期：2007-09-13 00:00:00
abstract：:Chromatin remodeling proteins regulate multiple aspects of cell homeostasis, making them ideal candidates for misregulation in transformed cells. Here, we explore Sin3A, a member of the Sin3 family of proteins linked to tumorigenesis that are thought to regulate gene expression through their role as histone deacetylas...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/onc.2012.326
更新日期：2013-06-27 00:00:00
abstract：:Polyamines are critical elements in mammals, but it remains unknown whether adenosyl methionine decarboxylase (AMD1), a rate-limiting enzyme in polyamine synthesis, is required for myeloid leukemia. Here, we found that leukemic stem cells (LSCs) were highly differentiated, and leukemia progression was severely impaire...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/s41388-020-01547-x
更新日期：2021-01-01 00:00:00
abstract：:The E6 proteins of specific cancer-associated human papillomaviruses (HPVs) complex with and mediate degradation of the cellular anti-oncogene p53 in vitro. A critical property of p53 is its ability to stimulate transcription from promoters containing its recognition sequence. HPV E6, mutant p53 proteins, and several ...
journal_title：Oncogene
pub_type： 杂志文章
doi：
更新日期：1995-02-02 00:00:00
abstract：:Cortactin, a p80/85 protein first identified as a src kinase substrate, is thought to be involved in the signaling pathway of mitogenic receptors and adhesion molecules mediating cytoskeletal reorganization. The cortactin gene, EMS1, maps to chromosome 11q13, a region amplified in head and neck squamous cell carcinoma...
journal_title：Oncogene
pub_type： 杂志文章
doi：10.1038/sj.onc.1201850
更新日期：1998-06-25 00:00:00
