Abstract:
:In order to assess the functional contribution of the human c-myc promoter region in the expression of the c-myc gene, transgenic mouse lines containing a bacterial lac Z gene encoding beta-galactosidase under the control of the human c-myc protooncogene promoter were generated. Transgenic mouse embryos heterozygous for the human c-myc Z transgene demonstrate high amounts of beta-galactosidase activity as early as day 11 of embryogenesis by histochemical staining of whole embryos using 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) as substrate, localizing specifically to early spinal cord tissue. beta-galactosidase activity can be demonstrated by histochemical staining in brain tissue of day 14 embryos, localizing mainly to the prefrontal cortex region, while relative amounts of beta-galactosidase in spinal cord tissue are reduced. Determination of specific activity of beta-galactosidase using resorufin-beta-galactopyranoside as substrate in homogenates of whole embryos heterozygous for the human c-myc/lac Z transgene demonstrates significantly elevated beta-galactosidase activity over control embryos in day 11 and day 14 embryos. Surprisingly, cell homogenates of brain tissue from adult G1 generation mice heterozygous for the human c-myc/lac Z transgene demonstrate greater than 10-fold higher specific activity of beta-galactosidase over normal control brain tissue. Specific inhibition of the c-myc/lac Z transgene was also demonstrated in developing embryos using mithramycin given at a dose of 150 micrograms kg-1 d-1 intraperitoneal to pregnant females on days 7-13 of gestation. Both histochemical staining of beta-galactosidase and specific activity assays of day 14 embryos demonstrated significantly lower levels of beta-galactosidase than untreated controls. These results are unique since we are able to detect expression of beta-galactosidase in developing embryonic central nervous system tissue along with adult brain tissue of animals carrying the human c-myc Z transgene and we are able to specifically inhibit expression of the transgene using mithramycin administered in utero.
journal_name
Oncogenejournal_title
Oncogeneauthors
Jones DE Jr,Cui DM,Miller DMsubject
Has Abstractpub_date
1995-06-15 00:00:00pages
2323-30issue
12eissn
0950-9232issn
1476-5594journal_volume
10pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::Recent evidence suggests that squamous cell carcinoma of the vulva may have more than one etiology, with only some tumors associated with human papillomavirus (HPV). Cells infected with HPV produce a viral protein (E6) which binds to and causes rapid degradation of p53, possibly contributing to cellular transformation...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Promyelocytic leukemia (PML) nuclear bodies (PML NBs) are discrete subnuclear domains organized by the promyelocytic leukemia protein PML, a tumor suppressor essential for multiple apoptotic pathways. We have recently described a novel family of cellular factors, the THAP proteins, characterized by the presence at the...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206271
更新日期:2003-04-24 00:00:00
abstract::Colon carcinogenesis is a multiple-step process involving the accumulation of a series of genetic and epigenetic alterations. The most commonly initiating event of intestinal carcinogenesis is mutation of the adenomatous polyposis coli (APC) gene, which leads to activation of the Wnt/β-catenin pathway. Olfactomedin 4 ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.58
更新日期:2016-10-06 00:00:00
abstract::We previously described the isolation of non-tumorigenic revertants from mutagenized populations of v-fos-transformed Rat-1 cells (Zarbl et al., 1987). In the present study we examined the possibility that the revertant phenotype resulted from mutations that altered the expression or activities of the c-jun or junB pr...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Resistance and relapse are still primary causes that result in poor effectiveness of chemotherapy in malignant gliomas. Therefore, development of new therapeutic strategies requires the identification of key molecular pathways regulating chemoresistance. We previously found that abnormal high expression of the Tie2 re...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.103
更新日期:2009-06-18 00:00:00
abstract::The naevoid basal cell carcinoma syndrome (NBCCS) is an autosomal dominant disorder characterized by multiple developmental defects and cancer susceptibility, in particular to basal cell carcinomas (BCCs). Medulloblastomas, primitive neuroectodermal tumours (PNETs) arising in childhood, occur in about 3-5% of NBCCS pa...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201340
更新日期:1997-07-17 00:00:00
abstract::Emerging evidence has shown that cancer stem cells (CSCs) are the cellular determinants to promote cancer invasion and metastasis. However, the mechanism underlying CSC invasion remains unknown. MicroRNAs are evolutionally conserved small noncoding RNAs that are critical for the regulation of gene expression, and thei...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.75
更新日期:2015-03-12 00:00:00
abstract::Augmented reactive oxygen species levels consequential to functional alteration of key mitochondrial attributes contribute to carcinogenesis, either directly via oxidative DNA damage infliction or indirectly via activation of oncogenic signaling cascades. We previously reported activation of a key oncogenic signaling ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.346
更新日期:2017-03-30 00:00:00
abstract::The NF-kappa B precursor p100 (lyt-10, p97, p98) generates after proteolytic processing a 52 kDa subunit, which can bind to kappa B-motifs. A deregulated form of the p100 gene, which is structurally altered by a t(10;14) translocation, has a potential oncogenic role in certain human B cell lymphomas. In this study p10...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::Upstream stimulating factor (USF2) is a basic helix-loop-helix leucine zipper transcription factor, which is found in most tissues. A critical role for USF2 in cellular proliferation has been proposed based on its importance in the regulation of various cyclins and P53 and its capability to antagonize c-myc. In this p...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201584
更新日期:1998-02-12 00:00:00
abstract::Although it has been demonstrated that transformed progenitor cell population can contribute to tumor initiation, factors contributing to this malignant transformation are poorly known. Using in vitro and xenograft-based models, previous studies demonstrated that miR-489 acts as a tumor suppressor miRNA by targeting v...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0439-1
更新日期:2019-01-01 00:00:00
abstract::B-Myb, a highly conserved member of the Myb oncoprotein family, is a 110 kDa sequence-specific DNA binding protein expressed in virtually all proliferating cells. B-myb expression reaches its maximum at the G1/S phase boundary and during the S phase of the cell cycle. We have previously shown that B-Myb activity is ce...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203618
更新日期:2000-06-15 00:00:00
abstract::We have identified sequences in the 5' flanking region of the murine c-myc gene's P1 and P2 transcription initiation sites which form specific complexes with nuclear factors of murine and human origin and are also required for normal P1 and P2 usage. Four nuclear factor binding sites were identified within 400 bp 5' o...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::Antimicrobial peptides, such as the cathelicidin LL-37/hCAP-18 and its mouse homolog cathelicidin-related antimicrobial peptide (CRAMP), are important effectors of the innate immune system with direct anti-bacterial activity. Cathelicidin is possibly involved in the regulation of tumor cell growth. The aim of this stu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.248
更新日期:2014-05-22 00:00:00
abstract::Histone deacetylase (HDAC) inhibitors induce growth arrest and apoptosis in a variety of human cancer cells. Sodium butyrate (NaB), a short chain fatty acid, is a HDAC inhibitor and is produced in the colonic lumen as a consequence of microbial degradation of dietary fibers. In order to dissect out the mechanism of Na...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207852
更新日期:2004-08-19 00:00:00
abstract::Radioresistance becomes the major obstacle to reduce tumor recurrence and improve prognosis in the treatment of esophageal squamous cell carcinoma (ESCC). Thus new strategies for radioresistant ESCC are urgently needed. Herein, we reported that tribbles pseudokinase 3 (TRIB3) serves as a key regulator of radioresistan...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-020-1245-0
更新日期:2020-04-01 00:00:00
abstract::Mutant p53 proteins were shown to exert complex DNA-interactions in vitro, like binding to MAR-DNA, but so far it is unknown whether such interactions also occur in vivo. Therefore we analysed the binding of mutant (mut) p53 (Gly245-->Ser) in Onda 11 glioma cells to cellular DNA in vivo, using p53-specific chromatin i...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203745
更新日期:2000-08-24 00:00:00
abstract::Aberrant activation of anaplastic lymphoma kinase (ALK) can cause sporadic and familial neuroblastoma. Using a proteomics approach, we identified Bruton's tyrosine kinase (BTK) as a novel ALK interaction partner, and the physical interaction was confirmed by co-immunoprecipitation. BTK is expressed in neuroblastoma ce...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0397-7
更新日期:2018-11-01 00:00:00
abstract::The receptor tyrosine kinase FLT3 is expressed in myeloid and lymphoid progenitor cells. Activating mutations in FLT3 occur in 25-30% of acute myeloid leukaemia (AML) patients. Most common are internal tandem duplications of sequence (ITD) leading to constitutive FLT3-ITD kinase activity with an altered signalling qua...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0757-y
更新日期:2019-06-01 00:00:00
abstract::The cdk2 gene has been identified as a human cdc2/CDC28-related gene that encodes a protein kinase essential for the G1/S transition in mammalian cells, but not for the G2/M transition, which requires Cdk1, another p34cdc2/CDC28 homolog. Novel potential functions of Cdk2 have been uncovered by using two potent and spe...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203103
更新日期:1999-12-09 00:00:00
abstract::We have recently isolated the erythroleukemic cell line, HB60-5, that proliferates in the presence of erythropoietin (Epo) and stem cell factor (SCF), but undergoes terminal differentiation in the presence of Epo alone. Ectopic expression of the ets related transcription factor Fli-1 in these cells resulted in the est...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203590
更新日期:2000-05-04 00:00:00
abstract::The molecular interactions implicated in the mammalian G1/S cell cycle phase transition comprise a highly nonlinear network which can produce seemingly paradoxical results and make intuitive interpretations unreliable. A new approach to this problem is presented, consisting of (1) a convention of unambiguous reaction ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201608
更新日期:1998-02-26 00:00:00
abstract::Colitis-associated colorectal cancers are an etiologically distinct subgroup of colon cancers that occur in individuals suffering from inflammatory bowel disease and arise as a consequence of persistent exposure of hyperproliferative epithelial stem cells to an inflammatory microenvironment. An intrinsic defect in the...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.545
更新日期:2012-08-09 00:00:00
abstract::Viruses have been engineered to replicate selectively in cancer cells, based on a number of innovative principles. Several of these viruses have entered clinical trials and have proven relatively safe, and have shown evidence of efficacy. However, further research is required to enable these agents to function systemi...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209064
更新日期:2005-11-21 00:00:00
abstract::We have found that the differentiation inducer butyric acid causes the synthesis of a cellular protein(s) that mediates a rapid decline in the level of myc RNA in SW837, a cell line derived from a human adenocarcinoma of the rectum. This effect was dose-dependent and was maximal at 1 mM. Among the short chain fatty ac...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::In the published version of this paper the author Shu-Pin Huang's surname was incorrectly given as Hwang instead of Huang. This has now been corrected in the HTML and PDF versions of the paper. ...
journal_title:Oncogene
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41388-018-0561-0
更新日期:2019-02-01 00:00:00
abstract::Melanoma is a deadly form of skin cancer that accounts for a disproportionally large proportion of cancer-related deaths in younger people. Compared with most other skin cancers, a feature of melanoma is its high metastatic capacity, although the mechanisms that confer this are not well understood. The Hippo pathway i...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-020-1362-9
更新日期:2020-07-01 00:00:00
abstract::cDNA clones of the ski-related gene, sno, were isolated from a chicken cDNA library and sequenced. In contrast to the human system, from which two forms of sno cDNAs have been isolated, we obtained only one type of chicken sno cDNA, that encoding snoN. The coding region for chicken snoN was inserted into the retrovira...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::Cells are under constant attack from genotoxins and rely on a multifaceted DNA damage response (DDR) network to maintain genomic integrity. Central to the DDR are the ATM and ATR kinases, which respond primarily to double-strand DNA breaks (DSBs) and replication stress, respectively. Optimal ATR signaling requires the...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.118
更新日期:2016-02-04 00:00:00
abstract::Cancer cells are known to adopt aerobic glycolysis in order to fuel tumor growth, but the molecular basis of this metabolic shift remains largely undefined. O-GlcNAcase (OGA) is an enzyme harboring O-linked β-N-acetylglucosamine (O-GlcNAc) hydrolase and cryptic lysine acetyltransferase activities. Here, we report that...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0975-3
更新日期:2020-01-01 00:00:00