Micropropagation of Hydrastis canadensis: Goldenseal a North American endangered species.

Abstract:

:An in vitro propagation protocol for rapidly producing Hydrastis canadensis L., Goldenseal, plantlets from disk tissue of young leaves was developed. Leaf explants were inoculated on MS medium supplemented with various concentrations of NAA and TDZ for production of callus. Two-month-old calli were sub-cultured on MS media containing cytokinins (BA, kinetin, TDZ) in different concentrations for shoot initiation. The optimum level of callus induction and maintenance was in 5.3 microM NAA in combination with 2.2 microM of TDZ. Shoot multiplication was achieved on MS medium with 2.2 microM TDZ in combination with 0.5 microM NAA. The alkaloid profile of micropropagated plantlets was similar to the profile of the mother plants. These results suggest that our in vitro propagation protocol will produce a positive impact in the conservation of H. canadensis.

journal_name

Planta Med

journal_title

Planta medica

authors

Bedir E,Lata H,Schaneberg B,Khan IA,Moraes RM

doi

10.1055/s-2003-37039

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

86-8

issue

1

eissn

0032-0943

issn

1439-0221

journal_volume

69

pub_type

信件
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