Abstract:
:An in vitro propagation protocol for rapidly producing Hydrastis canadensis L., Goldenseal, plantlets from disk tissue of young leaves was developed. Leaf explants were inoculated on MS medium supplemented with various concentrations of NAA and TDZ for production of callus. Two-month-old calli were sub-cultured on MS media containing cytokinins (BA, kinetin, TDZ) in different concentrations for shoot initiation. The optimum level of callus induction and maintenance was in 5.3 microM NAA in combination with 2.2 microM of TDZ. Shoot multiplication was achieved on MS medium with 2.2 microM TDZ in combination with 0.5 microM NAA. The alkaloid profile of micropropagated plantlets was similar to the profile of the mother plants. These results suggest that our in vitro propagation protocol will produce a positive impact in the conservation of H. canadensis.
journal_name
Planta Medjournal_title
Planta medicaauthors
Bedir E,Lata H,Schaneberg B,Khan IA,Moraes RMdoi
10.1055/s-2003-37039subject
Has Abstractpub_date
2003-01-01 00:00:00pages
86-8issue
1eissn
0032-0943issn
1439-0221journal_volume
69pub_type
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