Abstract:
:Thyroid hormones increase bone turnover in vivo and stimulate bone resorption in vitro. Clinical states associated with excess circulating thyroid hormone levels are known to produce osteoporosis. To determine the effect of T3 on bone resorption, we used an isolated rat osteoclast bone resorption assay in the absence or presence of added osteoblasts. This makes it possible to distinguish between direct and indirect effects of thyroid hormones on osteoclasts. In short settlement osteoclast cultures, which contain relatively few osteoblasts, 24-h treatment with T3 (10(-10)-10(-8) M) produced no stimulation of bone resorption. However, after 48-h incubation in the presence of T3, an increase in resorption was observed (2.3-fold at 10(-9) M). In cocultures of osteoclasts and osteoblasts (UMR 106-01 osteoblast-like cells or long settlement cultures), T3 stimulated resorption at 24 h. Furthermore, stimulation of resorption occurred when osteoblasts (UMR 106-01 or rat calvarial cells) were pretreated with T3 and the subsequent osteoblast-osteoclast cocultures conducted for 24 h in the absence of T3. Thus, direct exposure of osteoclasts to T3 was not required for the stimulatory effect. Treatment for 48 h with T3 (10(-9) M) or PTH (10(-8) M) had no effect on bone resorption in osteoblast-free cultures derived from human osteoclastoma tumours. T4 was 100-fold less potent than T3 as a stimulator of osteoclast activity, and rT3 had no effect. T3-induced stimulation was inhibited by salmon calcitonin (10(-10) M). These findings indicate that thyroid hormone can act on osteoblasts to indirectly stimulate osteoclastic bone resorption.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Britto JM,Fenton AJ,Holloway WR,Nicholson GCdoi
10.1210/endo.134.1.8275930subject
Has Abstractpub_date
1994-01-01 00:00:00pages
169-76issue
1eissn
0013-7227issn
1945-7170journal_volume
134pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::It was previously reported that PRL can stimulate FSH release in immature female rats and PRL-deficient male dwarf mice. We have examined the effects of PRL-producing ectopic pituitary grafts on plasma FSH and LH levels in adult male golden hamsters during exposure to both long (14 h of light, 10 h of darkness) and sh...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-1-133
更新日期:1981-01-01 00:00:00
abstract::The rat S14 gene is synergistically regulated by thyroid hormone and carbohydrates. The 5'-flanking region of this gene contains both carbohydrate and thyroid hormone response elements (TREs). We recently located the carbohydrate response element (CHORE) between -1583 and -1069 bases from the start site of transcripti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.1.8319560
更新日期:1993-07-01 00:00:00
abstract::Several laboratories have reported the presence of an intracellular, high affinity [dissociation constant (Kd), 1-2 nM] antiestrogen-specific binding site in estrogen target tissues. In this report, we describe the binding properties and characteristics of an additional triphenylethylene antiestrogen-binding site in t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-5-1823
更新日期:1983-05-01 00:00:00
abstract::Early life exposure to endocrine-disrupting chemicals (EDCs) is an emerging risk factor for the development of obesity and diabetes later in life. We previously showed that prenatal exposure to the EDC tributyltin (TBT) results in increased adiposity in the offspring. These effects linger into adulthood and are propag...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00056
更新日期:2018-08-01 00:00:00
abstract::Altered leptin action has been implicated in the pathophysiology of heart failure in obesity, a hallmark of which is extracellular matrix remodeling. Here, we characterize the direct influence of leptin on matrix metalloproteinase (MMP) activity in primary adult rat cardiac fibroblasts and focus on elucidating the mol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1166
更新日期:2011-05-01 00:00:00
abstract::In an attempt to further define the optimum experimental conditions for the in vitro study of PRL endocrinology, the effect of extracellular leucine concentration on PRL secretion was assessed. Rat pituitary cells, maintained in short term monolayer culture, were washed free of serum and placed in Minimum Essential Me...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-1-80
更新日期:1984-01-01 00:00:00
abstract::Administration of GH increases both the tubular reabsorption of inorganic phosphate (Pi) and the plasma level of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. These two effects could be induced by a common mediator, possibly the GH-generated insulin-like growth factor 1 (IGF-1). In the present work, the influence of recomb...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-1-453
更新日期:1990-07-01 00:00:00
abstract::The short loop feedback effect of PRL was studied in young (4-5 months of age) and old (24-26 months of age) ovariectomized rats after a single iv injection of bovine PRL (bPRL, 500 micrograms/100 g BW) or BSA (500 micrograms/100 g BW). Blood samples were collected via intraatrial cannula every 20 min for assay of PRL...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-4-1452
更新日期:1983-10-01 00:00:00
abstract::The establishment of dominant ovarian follicles that are capable of ovulating fertilizable oocytes is a fundamental determinant of female fertility. This process is governed by pituitary gonadotropins as well as local ovarian factors. Within the follicle, estrogen acts in an autocrine/paracrine manner to enhance FSH a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0349
更新日期:2005-08-01 00:00:00
abstract::LH and FSH are composed of a common alpha-subunit and a noncovalently associated hormone-specific beta-subunit. Unassociated beta LH and beta FSH can be retained in the cisternae of endoplasmic reticulum (ER). This phenomenon is particularly evident in gonadotropes of castrated animals where beta-subunits are expresse...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.3.7867570
更新日期:1995-03-01 00:00:00
abstract::Spermatogenesis is sustained by a heterogeneous population of spermatogonia that includes the spermatogonial stem cells. However, the mechanisms underlying their establishment from gonocyte embryonic precursors and their maintenance thereafter remain largely unknown. In this study, we report that inactivation of the u...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00396
更新日期:2017-11-01 00:00:00
abstract::The sexually dimorphic extrahypothalamic arginine-vasopressin (AVP) projections from the bed nucleus of the stria terminalis to the lateral septum (LS) and lateral habenula (LHb) are denser in males than females and, in rats, require males' perinatal exposure to gonadal hormones but the absence of such exposure in fem...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1315
更新日期:2005-03-01 00:00:00
abstract::Previously, a human CYP3A4-transgenic (Tg-CYP3A4) mouse line was reported to exhibit enhanced metabolism of midazolam by cytochrome P450 3A4 (CYP3A4) expressed in small intestine. Here we show that expression of CYP3A4 and murine cyp3a and cyp2b was both age and sex dependent. CYP3A4 was expressed in the livers of mal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1248
更新日期:2005-07-01 00:00:00
abstract::An estrogen-responsive uterine proenzyme of a proteinase in the immature rat uterus has been known for some time. Its mol wt is 77,000, its N-terminal amino acid sequence is the same as prothrombin's for 15 residues, it contains gamma-carboxyl glutamate residues, its biosynthesis is prevented by warfarin, it cross-rea...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-1-167
更新日期:1990-01-01 00:00:00
abstract::The quantities of estrone and estradiol were determined by radioimmunoassay in maternal and fetal plasma of the rhesus monkey from day 59 to 163 of gestation. A two way analysis of variance of data classified according to fetal sex and 3 pooled gestational ages for each hormone and for mother or fetus (4 analyses) rev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-2-425
更新日期:1975-08-01 00:00:00
abstract::Estrogen or raloxifene (LY156758) prevent estrogen deficiency-induced bone loss in animals and humans. We demonstrated in the rat that a 22% reduction in bone mineral density generated by ovariectomy was associated with a 2-fold reduction of transforming growth factor-beta 3 (TGF beta 3) messenger RNA expression in th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.5.8612550
更新日期:1996-05-01 00:00:00
abstract::The present work aimed to determine the role played by protein kinase-C (PKC) in the alpha 1-adrenoceptor-induced activation of hepatic metabolism. The following observations indicate that activation of PKC is a condition necessary for alpha 1-adrenoceptor activation of hepatic functions, but not sufficient to mimic t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8404660
更新日期:1993-11-01 00:00:00
abstract::Newborn male rats were treated SC with dexamethasone phosphate (100 microgram) or dexamethasone acetate (100 microgram) on postnatal day 1, dexamethasone phosphate (5.0 microgram) opn postnatal days 1, 2, 3, or the corresponding drug vehicle. All animals were sacrificed 65-70 days later at previously determined daily ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-3-1055
更新日期:1981-03-01 00:00:00
abstract::A study was conducted to determine the role of the thyroid gland in three neuroendocrine responses to photoperiod; secretion of melatonin, PRL, and LH. Ewes were thyroidectomized (THX) in midsummer or left thyroid intact, and both groups were moved indoors to artificial short days (8 h of light, 16 h of darkness) for ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013340
更新日期:1994-07-01 00:00:00
abstract::To identify factors that regulate the tempo of growth and puberty, the present study examined how the environment influenced the timing of menarche and first ovulation in rhesus monkeys and how these events were related to differential rates of growth. Spring-born females were raised from 12 months of age under natura...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-6-2653
更新日期:1988-12-01 00:00:00
abstract::Adiponectin (ApN) is an adipokine whose expression and plasma levels are inversely related to obesity and insulin-resistant states. Chronic repercussions of ApN treatment or overexpression on adiposity and body weight are still controversial. Here, we generated a transgenic (Tg) mouse model allowing persistent and mod...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0838
更新日期:2007-04-01 00:00:00
abstract::The testis contains many peptides originally described as originating in the central nervous system. The physiological function of these factors in the testis is generally unknown. We previously reported that the rat testis contains both a peptide with GH-releasing hormone-like immunoactivity (tGHRH-LI) and a mRNA spe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-5-2336
更新日期:1990-11-01 00:00:00
abstract::Follistatin (FS), which binds to the inhibin/activin beta A- or beta B-subunit is localized with and modulates the biological actions of activin in many systems. However, in contrast to the wide distribution of the activin beta-subunit proteins and messenger RNAs (mRNA) in the brain, demonstration of FS mRNA signal ha...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.5.8612560
更新日期:1996-05-01 00:00:00
abstract::For 3 yr, 1-yr synchronized circannual rhythms were found for the chick oviduct weights, amounts of soluble protein, concentrations of the progesterone receptor in this organ, and capacity of this steroid-receptor complex in cytosol in bind in vitro to deoxyribonucleoprotein acceptor sites. By fitting the data with 1-...
journal_title:Endocrinology
pub_type: 历史文章,杂志文章
doi:10.1210/endo-107-4-1234
更新日期:1980-10-01 00:00:00
abstract::Transforming growth factor-alpha (TGF alpha) has been localized to the anterior pituitary, specifically to the lactotroph and somatotroph cell populations, by our previous studies. Since pituitary lactotrophs are known to undergo growth in response to estrogens, we have used an estradiol-induced pituitary hyperplasia/...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1534540
更新日期:1992-06-01 00:00:00
abstract::Progesterone released by cumulus cells surrounding the egg induces a Ca2+ influx into human sperm cells via the cationic channel of sperm (CatSper) Ca2+ channel and controls multiple Ca2+-dependent responses essential for fertilization. We hypothesized that chemical UV filters may mimic the physiological action of pro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1473
更新日期:2016-11-01 00:00:00
abstract::Glucocorticoids regulate hippocampal function in part by modulating gene expression through the glucocorticoid receptor (GR). GR binding is highly cell type specific, directed to accessible chromatin regions established during tissue differentiation. Distinct classes of GR binding sites are dependent on the activity o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1929
更新日期:2017-05-01 00:00:00
abstract::Exposure of rats to microsomal enzyme inducers perturbs thyroid hormone (TH) homeostasis through a variety of mechanisms. Glucuronidation is an important metabolic pathway for TH and is catalyzed by uridine diphosphate-dibenzo-glucuronosyltransferase (UGT) family proteins. Administration of 2,3,7,8-tetrachlorodibenzo-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0443
更新日期:2007-12-01 00:00:00
abstract::Mouse β-cell-specific reporter lines have played a key role in diabetes research. Although the rat provides several advantages, its use has lagged behind the mouse due to the relative paucity of genetic models. In this report we describe the generation and characterization of transgenic rats expressing a Renilla lucif...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1550
更新日期:2016-03-01 00:00:00
abstract::Estrogen receptor (ER) expression and regulation is vital to the correct functioning of the neuroendocrine brain. Islet-1 (Isl-1) is a LIM homeodomain-containing transcription factor that has been implicated in neuronal differentiation, is located in the hypothalamus, and can alter ER function in vitro. We have determ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0996
更新日期:2004-01-01 00:00:00