Abstract:
:Several laboratories have reported the presence of an intracellular, high affinity [dissociation constant (Kd), 1-2 nM] antiestrogen-specific binding site in estrogen target tissues. In this report, we describe the binding properties and characteristics of an additional triphenylethylene antiestrogen-binding site in the serum of rats. By using saturation and competitive binding analyses with [3H]tamoxifen as the radio-labeled ligand, we have determined that rat serum contains a relatively high affinity (Kd, 28 nM), protease-sensitive binding site that is specific for antiestrogens of the triphenylethylene type (clomiphene and nafoxidine). Since this serum site has little affinity for the benzythiophene antiestrogens (LY 117018 and 156758), we have chosen the name of triphenylethylene-antiestrogen binding site (TABS). The concentration of serum TABS (picomoles of [3H]tamoxifen bound per ml serum) is roughly the same in males and females but is significantly greater in both sexes at days 5, 10, and 15 of age (400-500 pmol/ml) than in newborn or adult animals (100-200 pmol/ml). Preliminary characterization studies indicated that the serum TABS is probably a serum lipoprotein. Separation of rat serum lipoproteins on potassium bromide density gradients revealed that the serum TABS migrated with rat low density lipoprotein (LDL). Furthermore, rat LDL purified by density gradient centrifugation has an affinity and specificity for [3H]tamoxifen that is similar to the TABS found in whole serum. In contrast, the rat liver intracellular TABS does not have these density gradient characteristics. These data suggest that the rat serum TABS is LDL; however, the role of this serum lipoprotein in the mechanism of action of antiestrogens remains to be determined.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Winneker RC,Guthrie SC,Clark JHdoi
10.1210/endo-112-5-1823subject
Has Abstractpub_date
1983-05-01 00:00:00pages
1823-7issue
5eissn
0013-7227issn
1945-7170journal_volume
112pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Testicular tissue of normal and hCG-stimulated European eels was incubated in vitro with tritiated progesterone or androstenedione as substrates. The following compounds were isolated and identified: 5 beta-androstane-3,17-dione; 17 beta-hydroxy-5 beta-androstan-3-one; androst-4-ene-3,11,17-trione (adrenosterone); 11 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-3-916
更新日期:1982-03-01 00:00:00
abstract::T4-binding globulin (TBG), the principal carrier of thyroid hormone in serum, is a glycoprotein containing 20% carbohydrate. The importance of the carbohydrate moiety has been previously studied by enzymatic deglycosylation, which showed that deglycosylated TBG retains its original immunological an T4-binding properti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-4-1614
更新日期:1986-04-01 00:00:00
abstract::A single injection of either natural (cortisol, corticosterone) or synthetic [dexamethasone (DEX), triamcinolone acetonide] glucocorticosteroids to estradiol-primed, withdrawn chicks, resulted in a dose-dependent increase in the relative rates of ovalbumin and conalbumin synthesis. The simultaneous injection of equal ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2384
更新日期:1985-06-01 00:00:00
abstract::Ractopamine [1-(4-hydroxyphenyl-2-(1-methyl-3-(4-hydroxyphenyl)propylamino)ethanol] enhances protein accretion in skeletal muscle (sm) of pigs. Experiments were conducted to elucidate fractional protein synthesis (FSR) and mRNA abundance for alpha-actin in sm of pigs fed a 16% protein diet containing 20 parts/million ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-6-3096
更新日期:1990-06-01 00:00:00
abstract::L-Asparaginase (ASNase), a drug widely used in the treatment of acute lymphoblastic leukemia, has been reported to decrease serum T4-binding globulin (TBG) levels, while results of serum albumin determinations were conflicting. This effect in vivo has been attributed to depressed liver protein synthesis, but this hypo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-3-1185
更新日期:1986-09-01 00:00:00
abstract::We analyzed the nature of the thyroid hormone-binding proteins in bullfrog plasma using N-bromoacetyl-[125I]T3 as an affinity labeling probe. Polyacrylamide gel electrophoresis under nondenaturing conditions of the bullfrog N-bromoacetyl-[125I]T3-labeled plasma proteins revealed two proteins with specific binding to T...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.5.8477670
更新日期:1993-05-01 00:00:00
abstract::Recent studies suggest that hyperinsulinemia and insulin resistance are linked to Alzheimer's disease (AD). In this study, we used Tg2576 transgenic (Tg) mice, a widely used transgenic mouse model for AD, to explore the relationship between increased amyloid beta-peptide (Abeta) and insulin resistance. When fed a high...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-1196
更新日期:2010-04-01 00:00:00
abstract::131I therapy is a widely accepted treatment for differentiated thyroid cancers which can accumulate iodide. We evaluated the efficiency of 131I therapy against tumors which are transfected with the Na+/I- symporter (NIS) gene. We transfected the rat NIS cDNA expression vector into malignantly transformed rat thyroid c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.10.5571
更新日期:1997-10-01 00:00:00
abstract::Subcutaneous implantation of a silastic tubing containing crystalline estradiol in castrated female rats results in a circadian rhythm of LH release. Under such conditions, blockade of serotonin (5-HT) biosynthesis by p-chlorophenylalanine (PCPA) completely inhibits the afternoon elevation of plasma LH. Gonadotropin p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-2-496
更新日期:1976-08-01 00:00:00
abstract::As4.1 cells are derived from a renin-expressing kidney tumor induced by tissue-specific oncogene-mediated tumorigenesis in transgenic mice. These cells express high levels of renin messenger RNA (mRNA) and synthesize prorenin and renin; they were therefore used as a model to further investigate the molecular biology o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.7.7789330
更新日期:1995-07-01 00:00:00
abstract::GH receptor (GHR) mediates the anabolic and metabolic effects of GH. We previously characterized a monoclonal antibody (anti-GHR(ext-mAb)) that reacts with subdomain 2 of the rabbit GHR extracellular domain (ECD) and is a conformation-specific inhibitor of GH signaling in cells bearing rabbit or human GHR. Notably, th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1336
更新日期:2011-12-01 00:00:00
abstract::In the rat a GH-binding protein (GHBP) exists that is derived from the GH receptor gene by an alternative messenger RNA splicing mechanism such that the transmembrane and intracellular domains of the GH receptor are replaced by a hydrophilic carboxy terminus. Previous immunohistochemical studies detailing the localiza...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.5.1374020
更新日期:1992-05-01 00:00:00
abstract::It has previously only been possible to assess osteoclastic bone resorption in intact bone, where other cell types may modify or mediate osteoclastic responses to environmental agents. We have recently developed techniques which enable us to measure bone resorption by osteoclasts extracted from bone and have used thes...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-1-234
更新日期:1985-01-01 00:00:00
abstract::To investigate the possibility that a common phenomenon might exist by which glucocorticoids influence responses to thyroid hormones, the cytosol levels of four proteins were measured after 2 days of treatment with L-T3 (T3; 50 micrograms/100 g. day 2) and/or cortisone (2 mg/100 g./day) in adult male hypothyroid rats....
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-2-653
更新日期:1982-08-01 00:00:00
abstract::Maternal and fetal metabolism of vitamin D was examined in term pregnant rabbits fed a normal diet and in those supplemented with a large amount of vitamin D3. Term pregnant rabbits (27--30 days of gestation) fed the normal diet showed lower levels of plasma calcium, 25-hydroxyvitamin D3 (250HD3), and 24,25-dihydroxyv...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-6-1950
更新日期:1982-06-01 00:00:00
abstract::The purpose of these experiments was to determine if the powerful effect of sodium chloride concentration on angiotensin II- and potassium-stimulated aldosterone secretion by isolated perfused adrenal glands is mediated by the sodium or chloride ion or by the obligatory change in osmolality. We used isolated Ringer's ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-4-1621
更新日期:1985-04-01 00:00:00
abstract::Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3), the predominant IGF carrier protein in circulation, is posttranslationally modified in vivo by IGFBP-3 protease(s) into a number of fragments. Based on the ascertained and predicted recognition sites for known IGFBP-3 proteases, FLAG-epitope tagged intact I...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.11.7781
更新日期:2000-11-01 00:00:00
abstract::Although nicotinic cholinergic agonists have functional effects on PC 12 cells, radioligand-binding sites have not been detected. We, therefore, studied PC 12 cells incubated in the presence of nerve growth factor (NGF) and determined that specific [3H]nicotine-binding sites were induced approximately 2.5-fold in the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.2.1733730
更新日期:1992-02-01 00:00:00
abstract::The effects of LH on glucose metabolism within cumulus cell-enclosed bovine oocytes were determined. Cumulus cell-enclosed bovine oocytes were matured in vitro (IVM) in control medium alone or supplemented with LH, FSH, or TSH, then individually assayed for the metabolism of D-[5-3H]glucose, D-[1-14C]glucose, D-[6-14C...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1446610
更新日期:1992-12-01 00:00:00
abstract::Isolated granulosa cells and theca from proestrous hamsters alone and in recombination, were cultured in order to study steroidogenic capacity of this tissue. Cells from medium size antral follicles (100-300 mum diam.) and large preovulatory follicles (500 plus mum diam.) were used. Steroids were measured by radioimmu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-3-694
更新日期:1975-03-01 00:00:00
abstract::We have recently reported that pretreatment with electroacupuncture (EA) at stomach meridian point 36 (St36) prevents the chronic cold-stress increase in the hypothalamus-pituitary-adrenal axis (HPA), an action that may be under central control. Given that treatment for stress-related symptoms usually begins after ons...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/EN.2015-1018
更新日期:2015-10-01 00:00:00
abstract::Peptide-YY (PYY) is a novel enteric peptide that is structurally related to pancreatic polypeptide and neuropeptide-Y. The objectives of the present experiments were to characterize the following aspects of PYY metabolism: the distribution of PYY in the canine gastrointestinal tract, the release of PYY in response to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-3-1438
更新日期:1989-03-01 00:00:00
abstract::The influence of sex hormones on the immune response to foreign antigens as well as self-antigens is now recognized. In this study, we investigated the influence of gender and sex hormones on the development of antibodies to double-stranded DNA in nonautoimmune C57BL/6J mice. Immunoglobulin G (IgG) anti-dsDNA antibodi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.6.7988450
更新日期:1994-12-01 00:00:00
abstract::Nitrogenous compounds were studied for their effect on cGMP levels in the thyroid. Several agents, including sodium nitrite and hydroxylamine, could generate a rise of over 100-fold in cGMP. At a concentration of 20 mM, HN2OH and NaNO2 significantly reduced cAMP levels previously generated by 0.5 mU/ml TSH. If the sli...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-105-3-697
更新日期:1979-09-01 00:00:00
abstract::The present study employed the dehydroepiandrosterone (DHA) loading test to determine if the placenta in baboons remains functional following fetectomy. A bolus iv injection of 100 mg DHA was given to 4 baboons prior to and at various intervals following fetectomy at midgestation. Blood samples were withdrawn over a 4...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-2-843
更新日期:1985-02-01 00:00:00
abstract::Antisera were raised against synthetic replicates of the carboxyl-terminal (C-terminal) fragment of the precursor to human GH-releasing factor (GRF) (pre-proGRF) whose structure was predicted from the complementary DNA cloned from a pancreatic tumor. These antisera were used along with antisera to human GRF itself to ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-156
更新日期:1986-01-01 00:00:00
abstract::Estradiol is known to inhibit antigen presentation in the vagina. We report here that TGFbeta mediates the action of estradiol on vaginal antigen presenting cells (APC). When vaginal APC from ovariectomized rats were incubated with increasing concentrations of TGFbeta1 and TGFbeta2 in the presence of ovalbumin-specifi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.8.8938
更新日期:2002-08-01 00:00:00
abstract::PTHrP is produced in vessels and acts as a local modulator of tone. We recently reported that PTHrP(1-34) is able to induce vasorelaxation in rat uterine arteries, but in pregnancy, this response is blunted and becomes strictly endothelium dependent. The present study aimed to get insights into the mechanisms involved...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0568
更新日期:2008-02-01 00:00:00
abstract::Bone morphogenetic protein 2 (BMP2) belongs to the transforming growth factor-β superfamily and plays a critical role in regulating ovarian follicle function. Currently, the role of BMP2 during cumulus expansion remains to be determined. The aim of this study was to investigate the effect of BMP2 on the regulation of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00436
更新日期:2017-10-01 00:00:00
abstract::A RT-PCR approach was used to clone and sequence the full-length growth hormone receptor (GHR) of a teleost fish, the turbot (Scophthalmus maximus). Total liver RNA was amplified by RT-PCR with degenerate primers designed in extracellular and cytoplasmic regions, and a single DNA fragment of 1100 bp was obtained. The ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8407
更新日期:2001-07-01 00:00:00