Abstract:
:To investigate the possibility that a common phenomenon might exist by which glucocorticoids influence responses to thyroid hormones, the cytosol levels of four proteins were measured after 2 days of treatment with L-T3 (T3; 50 micrograms/100 g. day 2) and/or cortisone (2 mg/100 g./day) in adult male hypothyroid rats. Pituitary GH was measured by an established RIA. Hepatic alpha 2U-globulin, a hepatic protein whose synthesis is dependent on androgens thyroid hormones, glucocorticoids, and GH, was measured by a newly devised RIA shown to be sensitive and specific for alpha 2U-globulin. Hepatic malic enzyme and tyrosine aminotransferase were measured by enzyme assay. T3 stimulated pituitary GH, hepatic alpha 2U-globulin, and hepatic malic enzyme, but not hepatic tyrosine aminotransferase. Cortisone stimulated pituitary GH, hepatic alpha 2U-globulin, and hepatic tyrosine aminotransferase, but not hepatic malic enzyme. The response to T3 and cortisone in combination was equal to or greater than the sum of the responses to each hormone for hepatic alpha 2U-globulin and pituitary GH, but not for hepatic malic enzyme and tyrosine aminotransferase. The responses of pituitary GH to each treatment expressed as a ratio to hypothyroid control +/- SEM were: saline, 1 +/- 0.4; T3, 85.2 +/ 2.7; cortisone, 16.9 +/- 2.3; and T3 plus cortisone 133.9 +/- 5.4. The responses of hepatic alpha 2U-globulin were: saline, 1 +/- 0.6; T3, 6.6 +/-0.5; cortisone, 14.3 +/- 3.0; and T3 plus cortisone, 26.8 +/- 3.0. These results showed cortisone to have varying effects on thyroid hormone-responsive proteins in hypothyroid rats. Although both pituitary GH and hepatic alpha 2U-globulin were simulated in an additive manner, T3 alone was a more potent stimulus of GH, whereas cortisone alone was a more potent stimulus of alpha 2U-globulin.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Shapiro LE,Sachchidananda Jdoi
10.1210/endo-111-2-653subject
Has Abstractpub_date
1982-08-01 00:00:00pages
653-60issue
2eissn
0013-7227issn
1945-7170journal_volume
111pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::A monoclonal antibody directed against bovine TSH was obtained by hybridoma technology. This antibody was specific for TSH and did not react with bovine LH and FSH. Affinity chromatography of crude TSH was performed on anti-TSH Sepharose. Bovine TSH was purified in a single step to near homogeneity by this technique, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-1-319
更新日期:1988-01-01 00:00:00
abstract::Carboxypeptidase H (CP-H, EC 3.4.17.10) removes C-terminal basic amino acids from insulin and many other peptide hormone intermediates in endocrine and neural tissues. CP-H was studied in two rat insulinoma-derived clonal beta-cell lines that were chosen in part because their profile of hormone production is restricte...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.3.1505450
更新日期:1992-09-01 00:00:00
abstract::Developmental exposures to phthalates are suspected to contribute to risk of metabolic syndrome. However, findings from human studies are inconsistent, and long-term metabolic impacts of early-life phthalate and phthalate mixture exposures are not fully understood. Furthermore, most animal studies investigating metabo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2019-00287
更新日期:2019-07-01 00:00:00
abstract::3-Iodothyronamine (T(1)AM) is an endogenous thyroid hormone derivative with unknown biosynthetic origins. Structural similarities have led to the hypothesis that T(1)AM is an extrathyroidal metabolite of T(4). This study uses an isotope-labeled T(4) [heavy-T(4) (H-T(4))] that can be distinguished from endogenous T(4) ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1254
更新日期:2012-11-01 00:00:00
abstract::Prenatal stress is associated with altered behavior and hypothalamic-pituitary-adrenal (HPA) axis function postnatally. Recent studies suggest that these outcomes are dependent on the timing of the prenatal stress. The majority of these studies have been carried out in male offspring. We hypothesized that a short peri...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0347
更新日期:2008-12-01 00:00:00
abstract::Previous studies have shown that cysteamine [2-aminoethanethiol (CSH)], given in vivo or in vitro, rapidly but reversibly depletes immunoreactive PRL from the anterior pituitary (AP) through a mechanism which, in vitro, does not appear to involve the dopamine (DA) receptor. In the present investigation, these effects ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-6-2161
更新日期:1983-12-01 00:00:00
abstract::Polyadenylated RNA, extracted from rat hypothalami, cross-hybridized with a RNA probe complementary in sequence to rat PTH (rPTH) messenger RNA (mRNA). Amplification of complementary DNA (cDNA) by the polymerase chain reaction also demonstrated the presence of rPTH mRNA in the rat hypothalamus and parathyroid gland. r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-5-2517
更新日期:1990-11-01 00:00:00
abstract::Regulation of the number of pituitary vasopressin (VP) receptors plays an important role in controlling pituitary responsiveness during alterations of the hypothalamic pituitary adrenal axis. The mechanisms regulating these VP receptors were studied by analysis of the effects of adrenalectomy and glucocorticoid admini...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.12.5580
更新日期:1997-12-01 00:00:00
abstract::Previous studies have shown that the adenylyl cyclase of intact granulosa cells from immature porcine follicles has a uniquely prolonged responsiveness to FSH and an atypically poor responsiveness to cholera toxin relative to other cells. The present studies were designed to determine if these characteristics were due...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-302
更新日期:1986-01-01 00:00:00
abstract::By acting in the brain, insulin suppresses food intake, whereas neuropeptide Y (NPY) has the opposite effect. Since fasting increases NPY gene expression in the hypothalamic arcuate nucleus (ARC) and also lowers circulating insulin levels, we hypothesized that the anorexiant effect of insulin could result from insulin...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-5-2645
更新日期:1991-05-01 00:00:00
abstract::We previously reported that RU486 can reverse progesterone (P)-induced suppression of the estrogen receptor (ER) in the uterus of pregnant rhesus monkeys, but we did not determine whether estrogen (E) could act through its receptor in the presence of P and RU486. To pursue this question, we treated spayed rhesus monke...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.4.8462480
更新日期:1993-04-01 00:00:00
abstract::PTH-related protein (PTHrP), the major mediator of hypercalcemia of malignancy, reduces tubular phosphate (Pi) reabsorption through its PTH-like renotropic actions. Another peptide detected in tumoral cells, transforming growth factor-beta (TGF beta), has been shown to considerably suppress the sodium-dependent Pi tra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.1.8391420
更新日期:1993-07-01 00:00:00
abstract::Neurons that produce kisspeptin play a critical role in reproduction. However, understanding the molecular physiology of kisspeptin neurons has been limited by the lack of an in vivo marker for those cells. Here, we report the development of a Kiss1-CreGFP knockin mouse, wherein the endogenous Kiss1 promoter directs t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1521
更新日期:2011-11-01 00:00:00
abstract::The hormonal requirements for lactogenesis were investigated using explant cultures of mammary tissue obtained from cows at 30-40 days prepartum. Hormones used were insulin, hydrocortisone, and prolactin, and parameters examined were radioactive acetate incorporation into fatty acids, secretory response ratings, and h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-4-1192
更新日期:1977-04-01 00:00:00
abstract::Maternal nutrition can affect the susceptibility of the offspring to metabolic disease later in life, suggesting that this period is a window of opportunity for intervention to reduce the risk of metabolic disease. Resveratrol, a natural polyphenol, has a wide range of beneficial properties including anti-obesogenic, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa090
更新日期:2020-08-01 00:00:00
abstract::Rabbit hearts perfused by the Langendorff technique were studied. The addition of ovine prolactin (NIH-P-S-10) to the perfusate in a concentration of 50 ng/ml produced rapid increases in both the amplitude and rate of contraction in 33 adult male hearts studied in winter. Prepubertal male animals showed no response, a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-4-1008
更新日期:1975-10-01 00:00:00
abstract::Studies in the rat and rabbit indicate that facilitatory effects of neuropeptide Y (NPY) as well as norepinephrine (NE) on LH and LHRH release are dependent on the presence of the ovarian steroid estrogen. However, we have previously found the NE and an alpha-1-adrenergic agonist are both stimulatory to pulsatile LHRH...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-2-1144
更新日期:1991-02-01 00:00:00
abstract::Recent in vitro and in vivo evidence shows that secretion of parathyroid hormone (PTH) and calcitonin (CT) is stimulated by beta-adrenergic agonists and inhibited by beta-adrenergic antagonists. To assess the possible roles of adrenal medullary or adrenergic nerve terminal catecholamines in calcium homeostasis, we hav...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-107-4-977
更新日期:1980-10-01 00:00:00
abstract::Prolonged exposure to elevated FFA levels has been shown to induce peripheral insulin resistance and to alter the beta-cell secretory response to glucose. To investigate the effects of FFAs on preproinsulin gene expression, we measured insulin release, cell content, and messenger RNA (mRNA) levels in rat islets after ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.9.6953
更新日期:1999-09-01 00:00:00
abstract::We examined the gene expression of V-1, a novel soluble protein with the cdc10/SWI6 motif, in pseudopregnant rat ovaries. Northern blot analysis on days 1, 5, and 11 of pseudopregnancy revealed an approximately 2-fold increase in the V-1 messenger RNA (mRNA) expression level on day 5 to that on day 1, and no significa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.4.8625920
更新日期:1996-04-01 00:00:00
abstract::The estrogenic and antiestrogenic actions of the geometric isomers of tamoxifen and 4-hydroxytamoxifen were determined in a PRL synthesis assay using primary cultures of dispersed immature rat pituitary gland cells. 4-Hydroxytamoxifen was 100 times more potent as an antiestrogen than tamoxifen. The cis isomer of tamox...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-4-1449
更新日期:1988-04-01 00:00:00
abstract::We have found notable sex difference in peptidylarginine deiminase (EC 3.5.3.15) in rat pituitaries. Pituitaries collected from 3-week to 6-month-old male rats, and those from 3-week-old females showed negligible enzyme activities. The enzyme activity of female pituitaries increased markedly by 2 months, and even furt...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-6-2666
更新日期:1989-06-01 00:00:00
abstract::The effect of methysergide (MES, 2.5 mg/100 g body wt), a serotonin antagonist, on prolactin release has been studied in lactating and ovariectomized rats. MES caused significant increases in prolactin release in both animals. Studies in ovariectomized, hypophysectomized rats indicate that this effect is not due to a ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-5-1096
更新日期:1975-11-01 00:00:00
abstract::The very low density lipoprotein/apolipoprotein-E receptor (VLDLR) is the newest member of the low density lipoprotein receptor (LDLR) family. Very little is known about VLDLR localization and regulation. Immunohistochemical analysis of human placenta with a specific polyclonal antibody detected VLDLR in syncytiotroph...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.1.7828550
更新日期:1995-01-01 00:00:00
abstract::Hydrogen peroxide acts as electron acceptor in the oxidative reactions (iodination and coupling) by which the thyroid hormones are formed. Regulation of the generation of H2O2 was studied in monolayer cultures of the FRTL-5 rat thyroid cell line and in primary monolayer cultures of porcine thyroid cells. Both cell typ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.1.1309340
更新日期:1992-01-01 00:00:00
abstract::Altered leptin action has been implicated in the pathophysiology of heart failure in obesity, a hallmark of which is extracellular matrix remodeling. Here, we characterize the direct influence of leptin on matrix metalloproteinase (MMP) activity in primary adult rat cardiac fibroblasts and focus on elucidating the mol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-1166
更新日期:2011-05-01 00:00:00
abstract::Split hand/foot malformation (SHFM) is syndromic ectrodactyly often associated with mental retardation and/or craniofacial defects. Several clinical reports previously described urogenital dysplasia such as micropenis, hypospadias, and small testis in SHFM patients. Genetic lesions in the Dlx5 and Dlx6 (Dlx5/6) locus ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1265
更新日期:2008-05-01 00:00:00
abstract::We studied the role of calcium in dopaminergic control of the neuroendocrine effects of neurotensin. In primary cultures of dispersed normal female rat anterior pituitary cells the interactions of dopamine and neurotensin were examined with reference to the rate of PRL release, the magnitude of 45Ca2+ uptake, the rate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-4-1948
更新日期:1990-10-01 00:00:00
abstract::The PTH activates both adenylate cyclase and a mechanism that increases membrane-associated protein kinase-C (PKC) activity. To define the hormone's PKC activation domain we have used a panel of PTH fragments and ROS 17/2 rat osteosarcoma cells as the target cells. PTH equally and maximally increased PKC activity in R...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.1.1727720
更新日期:1992-01-01 00:00:00
abstract::Linear growth results from proliferation and differentiation of chondrocytes within the growth plates and is regulated, in part, by the insulin-like growth factors (IGFs). IGF binding proteins (IGFBPs) also appear to play a significant, but yet unclear, role. To examine IGFBP production by chondrocytes, we isolated bo...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.7688290
更新日期:1993-08-01 00:00:00