当前位置： SCI文献检索 > CANCER期刊下所有文献 > Growth patterns of human neuroblastoma xenografts and their relationship to treatment outcome.

Growth patterns of human neuroblastoma xenografts and their relationship to treatment outcome.

Abstract：

BACKGROUND:Several investigators have reported the ability to establish xenografts in nude mice from children with neuroblastomas, but a correlation of prognosis with this establishment and the growth patterns of the neuroblastomas has not been reported. METHODS:Tumor specimens from 58 children with neuroblastomas were heterotransplanted into BALB/c nude mice. In 34 patients, heterotransplantation was done before therapy; in 24 patients, tumors were obtained after at least one course of chemotherapy or radiation therapy. The histology, cytogenetics, and growth characteristics of serial passages of the xenografts were studied. RESULTS:The engraftment rate was 34%. Neuroblastomas with diploid chromosome numbers did not engraft. Chromosomal abnormalities involving 1p were seen in more than 50% of the xenografts. Cytogenetic features were retained between original tumors and resultant xenografts. Xenografts could be established only from tumors with unfavorable histology, as defined by Shimada classification criteria. The histology of each xenograft line was strikingly similar, and each was highly undifferentiated. Engraftment rates, doubling times, and lag times did not vary appreciably between xenografts established from treated tumors compared with xenografts established from untreated tumors. There was no correlation between doubling or lag times and prognosis. Patients whose tumors engrafted had only a 5% 3-year survival rate. CONCLUSIONS:From these results, it appears that successful engraftment is the most important prognostic indicator for patients with neuroblastomas. Because of the commonality of the histologic features and the stability of the tumor clones from patients before and after heterotransplantation, these xenografts may be useful as an in vivo model for studying drug resistance and for designing treatment regimens.

journal_name

Cancer

journal_title

Cancer

authors

George BA,Yanik G,Wells RJ,Martin LW,Soukup S,Ballard ET,Gartside PS,Lampkin BC

doi

10.1002/1097-0142(19931201)72:11<3331::aid-cncr282

subject

Has Abstract

pub_date

1993-12-01 00:00:00

pages

3331-9

issue

eissn

0008-543X

issn

1097-0142

journal_volume

pub_type

杂志文章

在线工具

Growth patterns of human neuroblastoma xenografts and their relationship to treatment outcome.