Role of protein phosphorylation in EPO-mediated early signal transduction: analysis in the EPO-reactive cell line ELM-I-1 transfected with a c-fos-enhancer/promoter-luciferase reporter gene.


:To investigate the role of protein phosphorylation in the early phase of EPO-mediated signal transduction, we EPO-stimulated a murine erythroid cell line ELM-I-1 transformed by plasmids comprised of the c-fos enhancer/promoter linked to the luciferase gene. Using this reporter gene system, we previously showed that EPO-induced activation of the c-fos promoter can be detected rapidly and sensitively as an elevation of cellular luciferase activity. In this study, we first examined the role of protein tyrosine phosphorylation. The tyrosine phosphatase inhibitor orthovanadate not only induced luciferase activity by itself but enhanced the action of EPO. On the other hand, the tyrosine kinase inhibitors erbstatin and herbimycin suppressed the effect of EPO. Next, the role of protein kinase C (PKC) in the EPO response was assessed. The PKC activator phorbol myristate acetate (PMA) not only induced luciferase activity by itself but enhanced the action of Epo. On the other hand, the PKC inhibitor 1-(5-isoquinolynyl-sulfonyl)-2-methylpiperazine (H7) suppressed the effect of Epo and PMA, whereas a nonspecific protein kinase inhibitor, N-(2-Guanidinoethyl)-5-Isoquinolinesulfornamine (HA1004) inhibited the action of neither Epo nor PMA. Another known PKC inhibitor staurosporine (STSP) did not inhibit but rather enhanced the effect of Epo. This action of STSP was blocked by H7 but not by HA1004. These results suggest that the EPO-mediated early signal transduction pathway leading to c-fos expression involves protein-tyrosine phosphorylation, is modulated by tyrosine phosphatase activity and is positively regulated by PKC.


Eur J Haematol


Tsuda H,Huang RW,Takatsuki K




Has Abstract


1994-04-01 00:00:00












  • Evaluating prophylaxis of invasive fungal infections in patients with haematologic malignancies.

    abstract:OBJECTIVE:Patients with hematologic malignancies are at substantial risk of developing invasive fungal infections (IFI) that are associated with substantial morbidity and mortality. This article reviews the epidemiology, risk factors, and efficacy of antifungal prophylaxis in patients with hematologic malignancies. ME...

    journal_title:European journal of haematology

    pub_type: 杂志文章,评审


    authors: Maertens J

    更新日期:2007-04-01 00:00:00

  • Plasma and cellular pharmacokinetics of m-AMSA related to in vitro toxicity towards normal and leukemic clonogenic bone marrow cells (CFU-GM, CFU-L).

    abstract::Plasma and cellular pharmacokinetics of m-AMSA were investigated in 5 patients with acute leukemia, using HPLC. The pharmacokinetic data served as a guideline for in vitro toxicity tests on clonogenic bone marrow cells. m-AMSA was administered as a 3-hour intravenous infusion of 100 mg/m2. Median plasma and nucleated ...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Linssen P,Brons P,Knops G,Wessels H,de Witte T

    更新日期:1993-03-01 00:00:00

  • Kinetics of circulating selectin levels during bone marrow aplasia.

    abstract::We have studied the kinetics of plasma levels of circulating (c)selectins in 8 patients undergoing bone marrow or stem cell transplantation to gain estimates for the distribution and half-life of (c)selectins and to potentially identify an endothelial source of cP-selectin in patients who are deprived of platelets and...

    journal_title:European journal of haematology

    pub_type: 临床试验,杂志文章


    authors: Jilma B,Eichler HG,Becherer A,Brugger S,Kalhs P,Stohlawetz P,Kapiotis S,Kapiotis S

    更新日期:1998-07-01 00:00:00

  • Prophylaxis of cytomegalovirus infection with ganciclovir in allogeneic marrow transplantation.

    abstract::Cytomegalovirus (CMV) infection is one of the most common causes of morbidity and mortality after allogeneic marrow transplantation. We studied 14 consecutive CMV-seropositive patients adding ganciclovir (2.5 mg/kg i.v. every 8 hours for 7 days prior to transplant and 6 mg/kg three times a week after neutrophils becam...

    journal_title:European journal of haematology

    pub_type: 临床试验,杂志文章


    authors: Yau JC,Dimopoulos MA,Huan SD,Tarrand JJ,Spencer V,Spitzer G,Meneghetti CM,Wallerstein RO,Andersson BS,LeMaistre CF

    更新日期:1991-11-01 00:00:00

  • 1 alpha(OH)D3 (ETALPHA) treatment and receptor studies in 16 patients with chronic and myeloproliferative disorders.

    abstract::10 patients with CLL and 2 with CML were treated with gradually increasing doses of 1 alpha(OH)D3, up to 4 micrograms daily during 6 wk. 3 patients with preleukemia and 1 with myelofibrosis were treated with 2 micrograms daily of 1 alpha(OH)D3 for a prolonged period up to 17 wk. The treatment with 1 alpha (OH)D3 did n...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Wieslander SB,Mortensen BT,Binderup L,Nissen NI

    更新日期:1987-07-01 00:00:00

  • Heterogeneity of in vitro growth pattern of megakaryocyte progenitors (CFU-M) in myeloproliferative disorders.

    abstract::In groups of 26 patients with myeloproliferative disorders (MPD), 8 with chronic myelogenous leukaemia (CML); 8 with polycythaemia vera (PV); 10 with essential thrombocythaemia (ET); and 6 patients with reactive thrombocytosis (RT), we studied the growth characteristics of bone marrow CFU-M in agar culture. The bone m...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Hamaguchi H,Takano N,Sakamaki H,Enokihara H,Saito K,Furusawa S,Shishido H

    更新日期:1988-08-01 00:00:00

  • Reticulocyte crisis after splenectomy: evidence for delayed red cell maturation?

    abstract::In whole blood from splenectomized subjects (n = 8) in a steady state an increased number of reticulocytes was observed (14.0 +/- 7.8% versus 3.6 +/- 2.4% controls: p less than 0.05). Cell fractionation on density showed that reticulocytes, as in normals, are more or less confined to the least dense fraction; cell fra...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: De Haan LD,Werre JM,Ruben AM,Huls AH,de Gier J,Staal GE

    更新日期:1988-07-01 00:00:00

  • An increase in basophils in a case of acute myelomonocytic leukaemia associated with marrow eosinophilia and inversion of chromosome 16.

    abstract::A patient with acute myelomonocytic leukaemia (M4 subtype) with pericentric inversion of chromosome 16, inv(16)(p13q22), and a marked basophilia is described. Results from transmission electron microscopy suggested that the basophils were immature. Clinically, although leukaemic cells had a tendency to infiltrate a nu...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Matsuura Y,Sato N,Kimura F,Shimomura S,Yamamoto K,Enomoto Y,Takatani O

    更新日期:1987-11-01 00:00:00

  • BCR-ABL1 molecular remission after 90Y-epratuzumab tetraxetan radioimmunotherapy in CD22+ Ph+ B-ALL: proof of principle.

    abstract::Although targeted therapies are used increasingly in hematologic malignancies, we are unaware of any prior studies of radioimmunotherapy (RAIT) in B-acute lymphoblastic leukemia (ALL), even though this radiosensitive tumor expresses CD22, potentially a good target for this approach. Here, we report a patient with Phil...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Chevallier P,Bodet-Milin C,Robillard N,Eugene T,Menard A,Le Houerou C,Guillaume T,Delaunay J,Escoffre-Barbe M,Wegener WA,Goldenberg DM,Kraeber-Bodéré F

    更新日期:2013-12-01 00:00:00

  • Alkaline phosphatase-positive B cell lymphomas.

    abstract::Alkaline phosphatase (ALP) activity of 70 cases of non-Hodgkin's lymphomas of the B-cell type was studied. ALP activity was found in malignant lymphoma (ML), follicular, small cleaved cell (1/5 cases); ML, diffuse, small cleaved cell (3/13 cases); and mantle zone lymphoma (intermediate lymphocytic lymphoma) (2/2 cases...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Abe M,Nozawa Y,Wachi E,Tominaga K,Hojo H,Wakasa H

    更新日期:1988-09-01 00:00:00

  • The platelet proaggregating and potentiating effects of unfractionated heparin, low molecular weight heparin and heparinoid in intensive care patients and healthy controls.

    abstract::Heparin binds to platelets and can cause platelet proaggregating and potentiating effects, possibly causing thrombocytopenia, particularly in patients in intensive care with hyperaggregable platelets. In this study we compared the platelet proaggregating and potentiating effects of unfractionated heparin (UH), 2 low m...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Burgess JK,Chong BH

    更新日期:1997-04-01 00:00:00

  • Assessment of the value of treatment with granulocyte colony-stimulating factor in children with acute lymphoblastic leukemia: a randomized clinical trial.

    abstract::The present trial was designed to test the effects of G-CSF on the duration of the second phase of induction chemotherapy in children with newly diagnosed acute lymphoblastic leukemia (ALL). A total of 32 patients were assigned randomly to a group that received (14 patients; group A) or a group that did not receive (1...

    journal_title:European journal of haematology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Dibenedetto SP,Ragusa R,Ippolito AM,Lo Nigro L,Di Cataldo A,D'Amico S,Miraglia V

    更新日期:1995-08-01 00:00:00

  • Heat production rate in blood lymphocytes as a prognostic factor in non-Hodgkin's lymphoma.

    abstract::The heat production rate in peripheral blood lymphocytes was evaluated by direct calorimetry in 76 untreated adults with non-Hodgkin's lymphoma (NHL). Elevated values were recorded for 20 out of 54 patients with lymphomas of high or intermediate malignancy grade (37%) and for 1 out of 22 patients (5%) with low grade l...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Monti M,Brandt L,Ikomi-Kumm J,Olsson H

    更新日期:1990-11-01 00:00:00

  • Kappa deleting element as an alternative molecular target for minimal residual disease assessment by real-time quantitative PCR in patients with multiple myeloma.

    abstract:BACKGROUND AND OBJECTIVES:Minimal residual disease (MRD) assessment by PCR in multiple myeloma (MM) has several shortcomings, including the lack of a suitable target. Kappa deleting element (KDE) rearrangements occur in virtually all Ig-lambda B-cell malignancies and in 1/3 of Ig-kappa are not affected by somatic hyper...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Puig N,Sarasquete ME,Alcoceba M,Balanzategui A,Chillón MC,Sebastián E,Díaz MG,San Miguel JF,García-Sanz R

    更新日期:2012-10-01 00:00:00

  • FLAMSA reduced-intensity conditioning is equally effective in AML patients with primary induction failure as well as in first or second complete remission.

    abstract::Reduced-intensity conditioning regimens have demonstrated lower toxicity but increased relapse rates in the context of allogeneic hematopoietic stem cell transplantation (aSCT) for patients with acute myelogenous leukemia (AML). The FLAMSA- reduced-intensity conditioning (RIC) regimen, combining a cytoreductive and a ...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Holtick U,Shimabukuro-Vornhagen A,Chakupurakal G,Theurich S,Leitzke S,Burst A,Hallek M,von Bergwelt-Baildon M,Scheid C,Chemnitz JM

    更新日期:2016-05-01 00:00:00

  • A comparison of fluorine-18 fluoro-deoxyglucose PET and technetium-99m sestamibi in assessing patients with multiple myeloma.

    abstract:OBJECTIVE:The extent of disease in patients with multiple myeloma or related conditions may be difficult to assess. In previous small studies, both FDG-PET (PET) and Tc-99m sestamibi scans (MIBI) have identified sites of occult disease in myeloma. METHODS:We reviewed the results for patients at our institution who hav...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Mileshkin L,Blum R,Seymour JF,Patrikeos A,Hicks RJ,Prince HM

    更新日期:2004-01-01 00:00:00

  • Dominant-negative mutations of the Wilms' tumour predisposing gene (WT1) are infrequent in CML blast crisis and de novo acute leukaemia.

    abstract::To determine if mutations of the Wilms' tumor predisposing gene (WT1) are associated with haematological malignancies, we have investigated 65 cases of acute leukaemia, including 39 patients in blast crisis of chronic myeloid leukaemia (CML), by amplification of WT1 exons 7, 8 and 9 followed by single-strand conformat...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Carapeti M,Goldman JM,Cross NC

    更新日期:1997-05-01 00:00:00

  • Myeloid malignancies with acquired trisomy 21 as the sole cytogenetic change are clinically highly variable and display a heterogeneous pattern of copy number alterations and mutations.

    abstract:OBJECTIVES:Acquired trisomy 21 is one of the most common numerical abnormalities in acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), and MDS/MPN; however, little is known about its pathogenic impact, accompanying submicroscopic changes, and its relation to other clinical...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Larsson N,Lilljebjörn H,Lassen C,Johansson B,Fioretos T

    更新日期:2012-02-01 00:00:00

  • Antifungal therapy in patients with hematological malignancies: how to avoid overtreatment?

    abstract:UNLABELLED:Historically, treatment of invasive fungal infections (IFI) has consisted of amphotericin B. However, new therapeutic agents have recently been introduced. At the same time, the relatively low incidence of IFI and the progress in the diagnostic accuracy of IFI have made routine use of empirical antifungal th...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Cherif H,Kalin M,Björkholm M

    更新日期:2006-10-01 00:00:00

  • Predictors of inferior clinical outcome in patients with standard-risk multiple myeloma.

    abstract:INTRODUCTION:Outcome of patients with standard-risk (SR) multiple myeloma (MM) has improved; however, subsets of patients do worse than expected. We sought to identify the factors associated with inferior outcome. METHODS:We evaluated 51 patients with SR MM that received upfront autologous hematopoietic stem cell tran...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Badar T,Srour S,Bashir Q,Shah N,Al-Atrash G,Hosing C,Popat U,Nieto Y,Orlowski RZ,Champlin R,Qazilbash MH

    更新日期:2017-03-01 00:00:00

  • Abnormality of platelet size and T-lymphocyte proliferation in an autosomal recessive form of dyskeratosis congenita.

    abstract::Dyskeratosis congenita (DC) is a rare familial hematologic disorder that has various modes of inheritance. We have studied 2 siblings with DC. 1 sibling had thrombocytopenia, which responded to therapy with nandrolone decanoate and oxymetholone. Platelets were abnormally small, which indicates that a qualitatively abn...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Juneja HS,Elder FF,Gardner FH

    更新日期:1987-10-01 00:00:00

  • Immunological and molecular biological identification of a true case of T-hairy cell leukaemia.

    abstract::A hairy cell leukaemia (HCL) patient is presented in whom the peripheral blood mononuclear cells (PBMCs) carried suppressor T-cell markers (CD3+, CD2+, CD8+/CD4-, CD38+). Analysis of genomic DNA of PBMNC showed the presence of a monoclonal population of T cells, the T-cell receptor (TCR) beta-chain gene being rearrang...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Demeter J,Pálóczi K,Földi J,Hokland M,Hokland P,Benczúr M,Lehoczky D

    更新日期:1989-10-01 00:00:00

  • Two beta-globin cluster-linked polymorphic loci in thalassemia patients of variable levels of fetal hemoglobin.

    abstract:OBJECTIVE:To correlate different polymorphisms of the beta-globin cluster with fetal hemoglobin (HbF) level in beta-thalassemia and E-beta thalassemia patients. METHODS:Fifteen thalassemia patients, seven with high HbF and not requiring transfusion, eight with lower HbF and requiring transfusion were studied for beta-...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Bandyopadhyay S,Mondal BC,Sarkar P,Chandra S,Das MK,Dasgupta UB

    更新日期:2005-07-01 00:00:00

  • Feasibility of fludarabine added to VAD during induction therapy in multiple myeloma: a randomised phase II-study.

    abstract::Multiple myeloma (MM) is considered to be an essentially incurable haematological malignant disease, probably because of the existence of resistant clonal precursor cell with self-renewal capacity. Recent data have indicated that the myeloma cell hierarchy includes circulating clonal memory B cells, which differ consi...

    journal_title:European journal of haematology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Björkstrand B,Rasmussen T,Remes K,Gruber A,Pelliniemi TT,Johnsen HE

    更新日期:2003-06-01 00:00:00

  • Patient-specific analysis of FLT3 internal tandem duplications for the prognostication and monitoring of acute myeloid leukemia.

    abstract:OBJECTIVES:Internal tandem duplications (ITDs) of the fms-like tyrosine kinase 3 ( FLT3) gene occur in 13-35% of patients with acute myeloid leukemia (AML). FLT3-ITD is associated with poor clinical outcome and is an indication for allogeneic stem cell transplantation (allo-SCT). METHODS:To investigate FLT3-ITD length...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Schiller J,Praulich I,Krings Rocha C,Kreuzer KA

    更新日期:2012-07-01 00:00:00

  • MicroRNAs as components of regulatory networks controlling erythropoiesis.

    abstract::Over the last two decades, the role of microRNAs has been extensively investigated, and it has become clear that these small non-coding RNAs play an essential role in several biological processes including erythropoiesis and that their dysregulation is associated with pathologies. Recent technical innovations have con...

    journal_title:European journal of haematology

    pub_type: 杂志文章,评审


    authors: Azzouzi I,Schmugge M,Speer O

    更新日期:2012-07-01 00:00:00

  • Primary pancreatic lymphoma: Clinical presentation, diagnosis, treatment, and outcome.

    abstract::Primary pancreatic lymphoma (PPL) is a rare disease representing 0.1% of malignant lymphomas, which lacks well-defined diagnostic and therapeutic protocols. OBJECTIVES:To describe PPL clinical, diagnostic and histological characteristics, together with therapy and outcome, in a relatively large series of patients. M...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Facchinelli D,Sina S,Boninsegna E,Borin A,Tisi MC,Piazza F,Scapinello G,Maiolo E,Hohaus S,Zamò A,Merli M,Stefani PM,Mellone F,Basso M,Sartori R,Rusconi C,Parisi A,Manfrin E,Krampera M,Ruggeri M,Visco C,Tecchio C

    更新日期:2020-10-01 00:00:00

  • Expression of c-myc and c-ras oncogenes in the neoplastic and non-neoplastic cells of Hodgkin's disease.

    abstract::The oncogenes c-myc and c-ras are known to elicit a cooperative tumorigenicity. In this study we investigated their role in the pathogenesis of Hodgkin's disease. The expression of these oncogenes was determined in Hodgkin's disease patients by avidin-biotin peroxidase complex immunohistochemical staining and was comp...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Benharroch D,Yermiahu T,Geffen DB,Prinsloo I,Gopas J,Segal S,Aboud M

    更新日期:1995-09-01 00:00:00

  • Chronic lymphocytic leukemia: a retrospective study of 122 cases.

    abstract::During the period 1978-1982 in the three northernmost countries of Sweden all 143 patients with a registered diagnosis of chronic lymphocytic leukemia (CLL) were retrospectively analyzed. After re-evaluation, 122 cases remained in the study. The mean age was 71 years and the male/female ratio was 2.2:1. Sixty-one pati...

    journal_title:European journal of haematology

    pub_type: 杂志文章,多中心研究


    authors: Erlanson M,Osterman B,Jonsson H,Lenner P

    更新日期:1994-02-01 00:00:00

  • Is valganciclovir really effective in primary effusion lymphoma: case report of an HIV(-) EBV(-) HHV8(+) patient.

    abstract::Primary effusion lymphoma (PEL) is a human herpesvirus 8 (HHV8) associated lymphoproliferative disease characterized by effusions in body cavities, and lack of tumor mass. Valganciclovir is a treatment option in PEL, however, little is known about its clinical efficacy. Ganciclovir has been reported to be effective in...

    journal_title:European journal of haematology

    pub_type: 杂志文章


    authors: Ozbalak M,Tokatlı I,Özdemirli M,Tecimer T,Ar MC,Örnek S,Koroglu A,Laleli Y,Ferhanoglu B

    更新日期:2013-11-01 00:00:00