Abstract:
:A suitable model for the preclinical study of human platelet production in vivo has not been available. NOD/SCID mice were characterized as representing an efficient engraftment model for human hematopoietic stem cells, which resulted in the production of human platelets. Here, we evaluated in vivo human thrombopoiesis and ex vivo human platelet functions in NOD/SCID mice transplanted with human cord blood (CB) CD34(+) cells. Human platelets and human CD45(+) cells appeared in peripheral blood of NOD/SCID mice from 4 wk after transplantation. Human platelets produced in these mice showed CD62P expression and the activation of GPIIb/IIIa on human platelets on stimulation with an agonist. PEG-rHuMGDF (0, 0.5 and 5 microg/kg/d s.c.) was injected for 14 d into mice that had been confirmed to produce human platelets stably. The number of human platelets increased about twofold at 0.5 microg/kg/d and about fivefold at 5 microg/kg/d after 14 d. Withdrawal of PEG-rHuMGDF administration caused the human platelet count to return to the pretreatment level. Further, re-administration of PEG-rHuMGDF induced a similar human thrombopoietic response as it did on initial administration. These results suggest that NOD/SCID mice engrafted with human CB CD34(+) cells will be useful for the study of human platelet production in vivo.
journal_name
Eur J Haematoljournal_title
European journal of haematologyauthors
Suzuki K,Hiramatsu H,Fukushima-Shintani M,Heike T,Nakahata Tdoi
10.1111/j.1600-0609.2006.00783.xsubject
Has Abstractpub_date
2007-02-01 00:00:00pages
123-30issue
2eissn
0902-4441issn
1600-0609pii
EJH783journal_volume
78pub_type
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journal_title:European journal of haematology
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journal_title:European journal of haematology
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更新日期:2013-09-01 00:00:00
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更新日期:2007-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1600-0609.2005.00529.x
更新日期:2005-11-01 00:00:00
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