Abstract:
BACKGROUND:Aberrant DNA methylation at CpG islands within promoters is increasingly recognised as a common event in human cancers and has been associated with the silencing of important tumour suppressor genes. Epigenetic therapy using hypomethylating agents has demonstrated clinical effectiveness; the drugs azacitidine and decitabine have been approved for the treatment of MDS. METHOD:We investigated the association between global DNA methylation and clinical outcome in MDS. We evaluated 134 MDS bone marrow trephine biopsies (BMTB) by immunohistochemistry and compared the results with those from an age-matched group of normal BMTB. Immunohistochemistry was performed on paraffin-embedded sections using the anti-5-methylcytosine (5mc) antibody. RESULTS:Our results showed that the 5mc immunostaining score (M-score) of patients with MDS was higher than those of normal controls and that overall survival significantly correlated with global DNA methylation, age and IPSS score. Therefore, we found that patients with high levels of methylation had a shorter median overall survival (OS) compared with patients with lower levels. These immunohistochemistry results were confirmed by analysing global DNA methylation on LINE-1 sequences using the COBRA method and pyrosequencing. CONCLUSION:This study reports that global DNA methylation detected by immunohistochemistry predicts OS in MDS.
journal_name
Eur J Haematoljournal_title
European journal of haematologyauthors
Poloni A,Goteri G,Zizzi A,Serrani F,Trappolini S,Costantini B,Mariani M,Olivieri A,Catarini M,Centurioni R,Alesiani F,Giantomassi F,Stramazzotti D,Biagetti S,Alfonsi S,Berardinelli E,Leoni Pdoi
10.1111/ejh.12145subject
Has Abstractpub_date
2013-09-01 00:00:00pages
219-27issue
3eissn
0902-4441issn
1600-0609journal_volume
91pub_type
杂志文章abstract::Although acute myeloid leukemia (AML) mostly occurs in older patients, it could be seen in women of childbearing age. It is therefore not surprising that in some patients the management of AML will be complicated by a coexistent pregnancy. However, the association of leukemia and pregnancy is uncommon. Its incidence i...
journal_title:European journal of haematology
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journal_title:European journal of haematology
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journal_title:European journal of haematology
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doi:10.1111/j.1600-0609.1987.tb00161.x
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journal_title:European journal of haematology
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abstract::Monoclonal antibodies 8A and 62B1, recognizing plasma cell-associated antigens, were covalently linked to saporin 6, a ribosome-inactivating protein similar to the A-chain of ricin. Both immunotoxins were tested on target human cell lines U266 and Raji, on non-target K562 cell line and on myeloid CFU-GM progenitors. T...
journal_title:European journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1600-0609.1989.tb00105.x
更新日期:1989-03-01 00:00:00
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journal_title:European journal of haematology
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doi:10.1111/j.1600-0609.1996.tb01330.x
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journal_title:European journal of haematology
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journal_title:European journal of haematology
pub_type: 临床试验,杂志文章
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journal_title:European journal of haematology
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journal_title:European journal of haematology
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1600-0609.1994.tb01295.x
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journal_title:European journal of haematology
pub_type: 临床试验,杂志文章
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journal_title:European journal of haematology
pub_type: 杂志文章,多中心研究,随机对照试验
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journal_title:European journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1600-0609.1993.tb00083.x
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journal_title:European journal of haematology
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pub_type: 杂志文章,评审
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更新日期:2012-09-01 00:00:00
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journal_title:European journal of haematology
pub_type: 杂志文章
doi:10.1111/j.1600-0609.1990.tb00357.x
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journal_title:European journal of haematology
pub_type: 临床试验,杂志文章
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更新日期:2007-10-01 00:00:00