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Immunotoxins containing saporin 6 and monoclonal antibodies recognizing plasma cell-associated antigens: effects on target cells and on normal myeloid precursors (CFU-GM).

Abstract：

:Monoclonal antibodies 8A and 62B1, recognizing plasma cell-associated antigens, were covalently linked to saporin 6, a ribosome-inactivating protein similar to the A-chain of ricin. Both immunotoxins were tested on target human cell lines U266 and Raji, on non-target K562 cell line and on myeloid CFU-GM progenitors. The cloning efficiency and viability of target cells were strongly reduced by 8A-saporin 6 and 62B1-saporin 6 immunotoxins, with an ID50 up to 200,000-fold lower than free saporin 6, whilst the K562 non-target cell line was unaffected. Normal human myeloid precursors (CFU-GM) were inhibited by immunotoxins only to a limited extent. An application of this model for autologous bone marrow transplantation in multiple myeloma patients is proposed. Since no eradication of cloning target cells was achieved by a single immunotoxin, mixtures made with different antibodies could help to reach this goal.

journal_name

Eur J Haematol

journal_title

European journal of haematology

authors

Barbieri L,Dinota A,Gobbi M,Tazzari PL,Rizzi S,Bontadini A,Lemoli RM,Tura S,Stirpe F

doi

10.1111/j.1600-0609.1989.tb00105.x

subject

Has Abstract

pub_date

1989-03-01 00:00:00

pages

238-45

issue

3

eissn

0902-4441

issn

1600-0609

journal_volume

42

pub_type

杂志文章

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