Aberrant promoter methylation of Dab2 gene in myelodysplastic syndrome.

Abstract:

OBJECTIVES:Human Disabled-2 (Dab2), a putative tumor suppressor gene, is frequently down-regulated in human tumors. This study aims to explore the association between Dab2 methylation status and expression in newly diagnosed myelodysplastic syndrome (MDS) patients and patients who received 5-aza-2'-deoxycytidine (decitabine) treatment, so as to determine the effect of Dab2 in the pathogenesis of MDS. METHODS:Methylation-specific polymerase chain reaction and bisulfite sequencing were used to detect the methylation status of Dab2 gene. Dab2 expression was investigated by using fluorescence quantitation RT-PCR (FQRT-PCR) and western blot analysis. RESULTS:Hypermethylation of Dab2 gene was present in 50.6% of patients with MDS and was significantly correlated with the down-regulation of Dab2 mRNA and protein expression. There was a significant difference in methylation frequency between refractory anemia/refractory anemia with ringed sideroblasts/MDS associated with isolated del (5q) (RA/RARS/5q-) group (33.3%) and refractory anemia with excess blasts-1/-2 (RAEB-1/RAEB-2) group (73.3%). Significant difference was also observed between refractory cytopenia with multiline dysplasia group (37.8%) and RAEB-1/RAEB-2 group. In addition, higher frequency of hypermethylation was observed in intermediate-2-/high-risk group, compared to low-risk/intermediate-1-risk group (75.0% vs. 40.0%). Demethylating agent 5-aza-2'-deoxycytidine treatment could partly reverse hypermethylation and, hence, restore the expression of Dab2 gene. CONCLUSIONS:The Dab2 gene is inactivated in MDS in part by DNA methylation, and the suppression of Dab2 expression by DNA methylation may play a role in the development of MDS.

journal_name

Eur J Haematol

authors

Yang Y,Zhang Q,Xu F,Chang C,Li X

doi

10.1111/ejh.12014

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

469-77

issue

6

eissn

0902-4441

issn

1600-0609

journal_volume

89

pub_type

杂志文章
  • Hematopoietic stem cell transplantation for sickle cell disease: state of the science.

    abstract::Sickle cell disease (SCD) is an inherited disorder secondary to a point mutation at the sixth position of the beta chain of human hemoglobin resulting in the replacement of valine for glutamic acid. This recessive genetic abnormality precipitates the polymerization of the deoxygenated form of hemoglobin S inducing a m...

    journal_title:European journal of haematology

    pub_type: 杂志文章,评审

    doi:10.1111/ejh.12447

    authors: Talano JA,Cairo MS

    更新日期:2015-05-01 00:00:00

  • Antifungal therapy in patients with hematological malignancies: how to avoid overtreatment?

    abstract:UNLABELLED:Historically, treatment of invasive fungal infections (IFI) has consisted of amphotericin B. However, new therapeutic agents have recently been introduced. At the same time, the relatively low incidence of IFI and the progress in the diagnostic accuracy of IFI have made routine use of empirical antifungal th...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.2006.00712.x

    authors: Cherif H,Kalin M,Björkholm M

    更新日期:2006-10-01 00:00:00

  • Analysis of low and high grade B-cell lymphoma subtypes using semi-nested PCR and two primer sets.

    abstract::Immunoglobulin gene rearrangements in B-cell lymphoma subtypes may not always be detected by PCR if only one primer set is applied. We therefore analysed a range of low and high grade B-cell lymphoma subtypes for monoclonality using PCR, to determine appropriate primer selection strategies for routine diagnostic use. ...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.1997.tb00966.x

    authors: Essop MF,Blakolmer K,Close PM,Manuel YE,Cornelius S

    更新日期:1997-09-01 00:00:00

  • Acute myelogenous leukemia in an adult with thrombocytopenia with absent radii syndrome.

    abstract::Acute myelogenous leukemia with t(8;21)(q22;q22) developed in a 42-yr-old woman having thrombocytopenia with absent radii syndrome (TARS). Standard induction and postremission therapies were safely administered. With each successive chemotherapy, the onset of platelet recovery was not delayed, but peak platelet counts...

    journal_title:European journal of haematology

    pub_type: 杂志文章,评审

    doi:10.1034/j.1600-0609.2003.00054.x

    authors: Go RS,Johnston KL

    更新日期:2003-04-01 00:00:00

  • On mouse and man: neutrophil gelatinase associated lipocalin is not involved in apoptosis or acute response.

    abstract::Neutrophil gelatinase-associated lipocalin (NGAL) is a siderphore binding molecule present in the specific granules of neutrophils and induced in a variety of epithelial cells during inflammation. Its mouse orthologue, 24p3, is also an acute phase protein synthesized in the liver and adipose tissue during inflammation...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.2005.00511.x

    authors: Klausen P,Niemann CU,Cowland JB,Krabbe K,Borregaard N

    更新日期:2005-10-01 00:00:00

  • Responsiveness of cytogenetically discrete human myeloma cell lines to lenalidomide: lack of correlation with cereblon and interferon regulatory factor 4 expression levels.

    abstract::The introduction of novel immunomodulatory drugs (IMiDs) has dramatically improved the survival of patients with multiple myeloma (MM). While it has been shown that patients with specific cytogenetic subtypes, namely t(4;14), have the best outcomes when treated with bortezomib-based regimens, the relationship between ...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/ejh.12192

    authors: Greenberg AJ,Walters DK,Kumar SK,Rajkumar SV,Jelinek DF

    更新日期:2013-12-01 00:00:00

  • Ligation of CD200R by CD200 is not required for normal murine myelopoiesis.

    abstract::CD200R is an inhibitory receptor involved in the regulation of myeloid cells. It recruits Dok-1 and Dok-2, which are potent inhibitors of the Ras signalling pathway used by colony-stimulating factor (CSF) receptors. Dok-1/Dok-2 double knockout (DKO) mice develop leukaemia at 10-12 months of age. We investigated whethe...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.2007.00920.x

    authors: Rijkers ES,de Ruiter T,Buitenhuis M,Veninga H,Hoek RM,Meyaard L

    更新日期:2007-11-01 00:00:00

  • Intensive remission induction therapy for chronic myeloid leukemia in blast phase with a goal of post-remission bone marrow transplant--a pilot study.

    abstract::An intensive protocol utilizing mitoxantrone, high-dose cytarabine, vincristine, etoposide and methylprednisolone as induction therapy for chronic myeloid leukemia in blast transformation is described. Fourteen patients were treated, with a remission/second chronic phase achieved in 64%. None of the 3 patients older t...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.1996.tb00488.x

    authors: Lipton JH,Messner HA,Curtis JE,Atkins HL,Minden MD

    更新日期:1996-07-01 00:00:00

  • Sudden ventricular fibrillation and death during ibrutinib therapy-A case report.

    abstract::Ibrutinib is an oral inhibitor of Bruton tyrosine kinase approved for the treatment of chronic lymphocytic leukaemia, mantle cell lymphoma and refractory Waldenstrom's disease. It increases progression-free survival, overall survival, response rate. The most frequent adverse reactions, are increased risk in of bleedin...

    journal_title:European journal of haematology

    pub_type:

    doi:10.1111/ejh.13290

    authors: Bernardeschi P,Pirrotta MT,Del Rosso A,Fontanelli G,Milandri C

    更新日期:2019-10-01 00:00:00

  • Is valganciclovir really effective in primary effusion lymphoma: case report of an HIV(-) EBV(-) HHV8(+) patient.

    abstract::Primary effusion lymphoma (PEL) is a human herpesvirus 8 (HHV8) associated lymphoproliferative disease characterized by effusions in body cavities, and lack of tumor mass. Valganciclovir is a treatment option in PEL, however, little is known about its clinical efficacy. Ganciclovir has been reported to be effective in...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/ejh.12174

    authors: Ozbalak M,Tokatlı I,Özdemirli M,Tecimer T,Ar MC,Örnek S,Koroglu A,Laleli Y,Ferhanoglu B

    更新日期:2013-11-01 00:00:00

  • A novel mutation in a Turkish patient with Hermansky-Pudlak syndrome type 5.

    abstract::The Hermansky-Pudlak syndrome (HPS) is a rare genetically heterogeneous autosomal recessive disorder, characterized by tyrosinase-positive oculocutaneous albinism, platelet dysfunction and lysosomal ceroid lipofuscin storage. This is caused by defects in lysosome-related organelles. In humans eight different types of ...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.2007.01024.x

    authors: Korswagen LA,Huizing M,Simsek S,Janssen JJ,Zweegman S

    更新日期:2008-04-01 00:00:00

  • Primary osteomyelofibrosis and an XX-male genotype.

    abstract::A 62-yr-old man with two healthy daughters was diagnosed with osteomyelofibrosis. To our surprise, a female XX-karyotype was observed in bone marrow and confirmed in PHA-stimulated T-lymphocytes from peripheral blood. Further molecular genetic investigation revealed a submicroscopic translocation between the short arm...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/ejh.12555

    authors: Schanz J,Haase D,Steuernagel P,Shirneshan K,Bäsecke J

    更新日期:2015-09-01 00:00:00

  • Expression of complement regulatory proteins CR1, DAF, MCP and CD59 in haematological malignancies.

    abstract::Host cells are protected from the lytic effect of the complement system by complement regulatory proteins. This study was designed to investigate the expression of complement regulatory proteins on leukemic blasts which may be susceptible to the lytic effects of the complement system in the circulation. The surface ex...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1034/j.1600-0609.2000.80097.x

    authors: Guc D,Canpinar H,Kucukaksu C,Kansu E

    更新日期:2000-01-01 00:00:00

  • Gene transduction into murine primitive hematopoietic cells with 2-gene retroviral vectors using a Transwell coculture system.

    abstract::The present study aims at expressing a reporter gene in hematopoietic cells in vivo by introducing it into primitive hematopoietic cells with a 2-gene retroviral vector. Various constructs of retroviral vectors containing the human IL-2 receptor alpha chain gene (TAC) as the reporter and the neomycin phosphotransferas...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.1996.tb01378.x

    authors: Asami N,Germeraad WT,Fujimoto S,Nagai S,Izumi T,Katsura Y

    更新日期:1996-10-01 00:00:00

  • Plasma cell leukemia: from biology to treatment.

    abstract::Plasma cell leukemia (PCL) is a very aggressive and rare form of malignant monoclonal gammopathy characterized by the presence of plasmocytes in peripheral blood. It is classified as primary PCL occuring 'de novo', or as secondary PCL in patients with relapsed/refractory multiple myeloma. Primary PCL is a distinct cli...

    journal_title:European journal of haematology

    pub_type: 杂志文章,评审

    doi:10.1111/ejh.12533

    authors: Jelinek T,Kryukov F,Rihova L,Hajek R

    更新日期:2015-07-01 00:00:00

  • Characterization of a new human plasma cell leukemia cell line UHKT-944.

    abstract:OBJECTIVE:A new interleukin-6 (IL-6)-dependent plasma cell leukemia cell line UHKT-944 was established from bone marrow cells derived from a 55-yr-old man with plasma cell leukemia. RESULTS:The cell line possesses phenotypic characteristics of plasma cells including the production of a monoclonal immunoglobulin IgA1-k...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/ejh.12505

    authors: Vyhlídalová I,Uherková L,Pleschnerová M,Špička I,Březinová J,Michalová K,Čermáková K,Polanská V,Jedelský PL,Hamšíková E,Kuželová K,Stöckbauer P

    更新日期:2015-10-01 00:00:00

  • Hypoxia and deposition of iron in liver and spleen of mice given iron supplement.

    abstract::Iron-related changes in peripheral blood and variations in liver and spleen iron concentrations during alternating periods of hypoxia and normoxia have been investigated in iron-supplemented mice by chemical and histological methods. During hypoxia, packed cell volume increased from 40 to 70%. The iron content of the ...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.1990.tb00348.x

    authors: Borch-Iohnsen B,Myhre K,Norheim G

    更新日期:1990-01-01 00:00:00

  • G6PD deficiency and absence of α-thalassemia increase the risk for cerebral vasculopathy in children with sickle cell anemia.

    abstract::The aim of this study was to test the association between hematological/genetic factors and cerebral vasculopathy in children with sickle cell anemia (SCA). A group with cerebral vasculopathy (VASC) was composed of children who had stroke (n = 6), silent infarct (n = 11), or an abnormal transcranial Doppler (n = 5). E...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/ejh.12607

    authors: Joly P,Garnier N,Kebaili K,Renoux C,Dony A,Cheikh N,Renard C,Ceraulo A,Cuzzubbo D,Pondarré C,Martin C,Pialoux V,Francina A,Bertrand Y,Connes P

    更新日期:2016-04-01 00:00:00

  • Increasing incidence of primary central nervous system lymphoma but no improvement in survival in Sweden 2000-2013.

    abstract:OBJECTIVES:This study aims to characterize the epidemiology of immunocompetent Primary central nervous system lymphoma (PCNSL) diagnosed 2000-2013 in Sweden. METHODS:Cases were identified in the population-based Swedish Lymphoma Register. Incidence per 100 000 person-years and 95% confidence intervals (CI) were calcul...

    journal_title:European journal of haematology

    pub_type: 历史文章,杂志文章

    doi:10.1111/ejh.12980

    authors: Eloranta S,Brånvall E,Celsing F,Papworth K,Ljungqvist M,Enblad G,Ekström-Smedby K

    更新日期:2018-01-01 00:00:00

  • Deferiprone-associated myelotoxicity.

    abstract::Agranulocytosis developed in a 63-year-old patient with myelodysplasia 6 weeks after commencing treatment with the oral iron chelator deferiprone (L1, 1,2-dimethyl-3-hydroxypyrid-4-one, CP20) at a daily dose of 79 mg/kg. This was the 3rd case of agranulocytosis (neutrophils 0 x 10(9)/l) in clinical trials of L1 at the...

    journal_title:European journal of haematology

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1600-0609.1994.tb01323.x

    authors: al-Refaie FN,Wonke B,Hoffbrand AV

    更新日期:1994-11-01 00:00:00

  • An antecedent diagnosis of refractory anemia with excess blasts has no influence on mobilization of peripheral blood stem cells and hematopoietic recovery after autologous stem cell transplantation in acute myeloid leukemia.

    abstract::Several studies have reported data on factors influencing mobilization of peripheral blood stem cells (PBSC) in non-myeloid malignancies. On the contrary, data from patients with acute myeloid leukemia (AML) are very limited, in particular, as the impact of an antecedent diagnosis of refractory anemia with excess blas...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.2006.00777.x

    authors: Viola A,Falco C,D'Elia R,D'Amico MR,Vicari L,Tambaro FP,Correale P,Laudati D,Palmieri S,Ferrara F

    更新日期:2007-01-01 00:00:00

  • Antitumour activity of the glycoengineered type II anti-CD20 antibody obinutuzumab (GA101) in combination with the MDM2-selective antagonist idasanutlin (RG7388).

    abstract:OBJECTIVES:To investigate whether the glycoengineered type II anti-CD20 monoclonal antibody obinutuzumab (GA101) combined with the selective MDM2 antagonist idasanutlin (RG7388) offers superior efficacy to monotherapy in treating B-lymphoid malignancies in preclinical models. METHODS:The combined effect of obinutuzuma...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/ejh.12756

    authors: Herting F,Herter S,Friess T,Muth G,Bacac M,Sulcova J,Umana P,Dangl M,Klein C

    更新日期:2016-11-01 00:00:00

  • An overview of current trends and gaps in patient-reported outcome measures used in haemophilia.

    abstract:AIM:This review summarises the importance, recent progress and issues in measuring patient-reported outcomes (PROs) in haemophilia research. METHODS:A critical review of recent advances and trends in measuring haemophilia-related PROs was conducted, using current regulatory guidelines and methodological recommendation...

    journal_title:European journal of haematology

    pub_type: 杂志文章,评审

    doi:10.1111/ejh.12323

    authors: Pocoski J,Benjamin K,Michaels LA,Flood E,Sasane R

    更新日期:2014-06-01 00:00:00

  • PlGF and sFlt-1 levels in patients with non-transfusion-dependent thalassemia: Correlations with markers of iron burden and endothelial dysfunction.

    abstract:BACKGROUND:Levels of the angiogenic cytokines placental growth factor (PlGF) and soluble Fms-like tyrosine kinase-1 (sFlt-1) and the angiogenic balance, expressed by sFlt-1/PlGF ratio, are perturbed in sickle-cell disease and iron overload, but they have not been evaluated in non-transfusion-dependent thalassemia (NTDT...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/ejh.13061

    authors: Kelaidi C,Kattamis A,Apostolakou F,Poziopoulos C,Lazaropoulou C,Delaporta P,Kanavaki I,Papassotiriou I

    更新日期:2018-06-01 00:00:00

  • Clonal evolution in CLL patients as detected by FISH versus chromosome banding analysis, and its clinical significance.

    abstract::The acquisition of new aberrations during the course of chronic lymphocytic leukemia (CLL) named clonal evolution (CE) is usually detected by one of the two methods: chromosome banding analysis (CBA) and interphase fluorescence in situ hybridization (I-FISH). The purpose of this study was to compare the usefulness of ...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/ejh.12215

    authors: Wawrzyniak E,Kotkowska A,Blonski JZ,Siemieniuk-Rys M,Ziolkowska E,Giannopoulos K,Robak T,Korycka-Wolowiec A

    更新日期:2014-02-01 00:00:00

  • Haematopoietic response and bcl-2 expression in patients with acute myeloid leukaemia.

    abstract::Bcl-2 expression, the number of apoptotic cells and the growth and differentiation of early bone marrow progenitor cells were studied in patients with confirmed diagnosis of acute myeloid leukaemia (AML). Bone marrow cells from normal individuals were used as controls. We observed an increased percentage of bcl-2-mono...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.1999.tb01112.x

    authors: Bincoletto C,Saad ST,da Silva ES,Queiroz ML

    更新日期:1999-01-01 00:00:00

  • Survival and graft-versus-host disease in patients receiving peripheral stem cell compared to bone marrow transplantation from HLA-matched related donor: retrospective analysis of 334 consecutive patients.

    abstract:OBJECTIVES:The objective of this study was to compare the major transplant outcomes between patients receiving hematopoietic stem cell transplantation (HSCT) from bone marrow (BM) or peripheral blood stem cells (PBSC). METHODS:All consecutive HSCT patients using BM or PBSC from an HLA-matched related donors for haemat...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/ejh.12508

    authors: Campregher PV,Hamerschlak N,Colturato VA,Mauad MA,de Souza MP,Bouzas LF,Tavares Rde C,Barros JC,Chiattone R,Paz A,Silla L,Vigorito AC,Miranda E,Funke VA,Flowers ME

    更新日期:2015-11-01 00:00:00

  • Bone scan images reveal increased osteoblastic function after bortezomib treatment in patients with multiple myeloma.

    abstract::Osteolytic lesions with activated osteoclast (OC) and suppressed osteoblast (OB) activity are characteristics of myeloma bone lesion. Recently, it has been shown that bortezomib treatment enhances OB function. To evaluate the effect of bortezomib on myeloma bone lesions, we performed bone scans, where increased uptake...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.2010.01523.x

    authors: Lee SE,Min CK,Yahng SA,Cho BS,Eom KS,Kim YJ,Kim HJ,Lee S,Cho SG,Kim DW,Lee JW,Min WS,Park CW

    更新日期:2011-01-01 00:00:00

  • Prevalence and risk factors for Pulmonary Hypertension associated with chronic Myeloproliferative Neoplasms.

    abstract:OBJECTIVES:Pulmonary hypertension (PH) is commonly reported in Philadelphia-chromosome negative myeloproliferative neoplasms (MPNs) including polycythemia vera (PV), essential thrombocythemia (ET), and myelofibrosis (MF). PH may be diagnosed directly by right heart catheterization (RHC) or estimated by transthoracic ec...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/ejh.13543

    authors: Ferrari A,Scandura J,Masciulli A,Krichevsky S,Gavazzi A,Barbui T

    更新日期:2021-02-01 00:00:00

  • Two pathways for iron uptake by guinea pig reticulocytes.

    abstract::We have demonstrated that the intracellular processing of transferrin to effect iron removal involves two pathways, one sensitive to rotenone and the other not. We have also found that the effect of the rotenone is dependent on the transferrin concentration: iron uptake was suppressed with concentrations of transferri...

    journal_title:European journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1600-0609.1990.tb00408.x

    authors: Zhan H,Pollack S,Weaver J

    更新日期:1990-07-01 00:00:00