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Biological and clinical significance of in vitro prednisolone resistance in adult acute lymphoblastic leukaemia.

Abstract：

:It has been reported that in vitro prednisolone (PDN) resistance provides a prognostic value in childhood acute lymphoblastic leukaemia (ALL). This study aimed at investigating the biological and clinical significance of in vitro PDN resistance in adult ALL. Blast cells from 30 patients were exposed to PDN (0.1 microM-35 microM) and cytotoxicity was determined by the soluble tetrazolium formazan 2,3-bis (2-methoxy-4-nitro-5-sulphophenyl)-5-[(phenylamino) carbamyl]-2H-tetrazolium hydroxyde (XTT) colorimetric assay. The IC50 (defined as the drug concentration that results in 50% growth inhibition) varied greatly among the samples, from 0.3 microM to > 35 microM; 15 microM was subsequently chosen as IC50-cut-off point between in vitro resistant and sensitive cases. PDN-induced cytotoxicity was significantly related to apoptosis, as demonstrated by regression analysis; in sensitive cases, however, the percentage of apoptotic cells after in vitro PDN treatment was significantly increased compared with control (p = 0.002). Immunofluorescence evaluation of intracellular BCL-2 protein showed an equal percentage of positive cells in the two groups, but in resistant cells a higher mean fluorescence intensity (p = 0.04) was demonstrated. In vitro sensitive and resistant patients did not display differences in clinical characteristics, in cytological, karyotypic and immunophenotypic features and in the outcome of induction therapy. Disease-free survival (DFS), however, was significantly better in sensitive patients (p = 0.02).

journal_name

Eur J Haematol

journal_title

European journal of haematology

authors

Tosi P,Visani G,Ottaviani E,Manfroi S,Tura S

doi

10.1111/j.1600-0609.1996.tb01351.x

subject

Has Abstract

pub_date

1996-08-01 00:00:00

pages

134-41

issue

2

eissn

0902-4441

issn

1600-0609

journal_volume

57

pub_type

杂志文章

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