Designation by restriction fragment length polymorphism of major histocompatibility complex class IV haplotypes in meat-type chickens.

Abstract:

:Major histocompatibility complex (MHC) class IV haplotypes were identified in a population of meat-type chickens by restriction fragment length polymorphism (RFLP) analysis. Fourteen different haplotypes were designated on the basis of restriction patterns obtained from Southern blots of PvuII- or BglII-digested DNA, hybridized with the MHC class IV cDNA probe bg32.1. Digestion with each restriction enzyme yielded the same level of polymorphism among individuals. For each haplotype, 4-10 restriction fragments ranging from 0.8 to 8 kb were observed. Such a designation of meat-type chicken MHC class IV haplotypes enables a rapid recognition of previously defined haplotypes, is readily adjustable to additional, newly found restriction patterns and could prove useful in practical breeding programmes.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Landesman E,Uni Z,Heller ED

doi

10.1111/j.1365-2052.1993.tb00339.x

subject

Has Abstract

pub_date

1993-10-01 00:00:00

pages

349-54

issue

5

eissn

0268-9146

issn

1365-2052

journal_volume

24

pub_type

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