Abstract:
:Major histocompatibility complex (MHC) class IV haplotypes were identified in a population of meat-type chickens by restriction fragment length polymorphism (RFLP) analysis. Fourteen different haplotypes were designated on the basis of restriction patterns obtained from Southern blots of PvuII- or BglII-digested DNA, hybridized with the MHC class IV cDNA probe bg32.1. Digestion with each restriction enzyme yielded the same level of polymorphism among individuals. For each haplotype, 4-10 restriction fragments ranging from 0.8 to 8 kb were observed. Such a designation of meat-type chicken MHC class IV haplotypes enables a rapid recognition of previously defined haplotypes, is readily adjustable to additional, newly found restriction patterns and could prove useful in practical breeding programmes.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
Landesman E,Uni Z,Heller EDdoi
10.1111/j.1365-2052.1993.tb00339.xsubject
Has Abstractpub_date
1993-10-01 00:00:00pages
349-54issue
5eissn
0268-9146issn
1365-2052journal_volume
24pub_type
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