Abstract:
:Hepatitis E virus (HEV) replication is not well understood, mainly because the virus does not infect cultured cells efficiently. However, Huh-7 cells transfected with full-length genomes produce open reading frame 2 protein, indicative of genome replication (6). To investigate the role of 3'-terminal sequences in RNA replication, we constructed chimeric full-length genomes with divergent 3'-terminal sequences of genotypes 2 and 3 replacing that of genotype 1 and transfected them into Huh-7 cells. The production of viral proteins by these full-length chimeras was indistinguishable from that of the wild type, suggesting that replication was not impaired. In order to better quantify HEV replication in cell culture, we constructed an HEV replicon with a reporter (luciferase). Luciferase production was cap dependent and RNA-dependent RNA polymerase dependent and increased following transfection of Huh-7 cells. Replicons harboring the 3'-terminal intergenotypic chimera sequences were also assayed for luciferase production. In spite of the large sequence differences among the 3' termini of the viruses, replication of the chimeric replicons was surprisingly similar to that of the parental replicon. However, a single unique nucleotide change within a predicted stem structure at the 3' terminus substantially reduced the efficiency of replication: RNA replication was partially restored by a covariant mutation. Similar patterns of replication were obtained when full-length genomes were inoculated into rhesus macaques, suggesting that the in vitro system could be used to predict the effect of 3'-terminal mutations in vivo. Incorporation of the 3'-terminal sequences of the swine strain of HEV into the genotype 1 human strain did not enable the human strain to infect swine.
journal_name
J Viroljournal_title
Journal of virologyauthors
Graff J,Nguyen H,Kasorndorkbua C,Halbur PG,St Claire M,Purcell RH,Emerson SUdoi
10.1128/JVI.79.2.1017-1026.2005subject
Has Abstractpub_date
2005-01-01 00:00:00pages
1017-26issue
2eissn
0022-538Xissn
1098-5514pii
79/2/1017journal_volume
79pub_type
杂志文章abstract:UNLABELLED:Human immunodeficiency virus type 1 (HIV-1) infection in the central nervous system (CNS) is characterized by replication in macrophages or brain microglia that express low levels of the CD4 receptor and is the cause of HIV-associated dementia and related cognitive and motor disorders that affect 20 to 30% o...
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doi:10.1128/JVI.73.5.3758-3763.1999
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pub_type: 杂志文章
doi:10.1128/JVI.65.9.5037-5044.1991
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.1808-1813.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.1040-1049.1992
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pub_type: 杂志文章
doi:10.1128/JVI.63.7.3128-3134.1989
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5636-5640.1991
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.10.6567-6571.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2330-2337.2003
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.6.1357-1366.1975
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journal_title:Journal of virology
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journal_title:Journal of virology
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doi:10.1128/JVI.46.2.405-412.1983
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journal_title:Journal of virology
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pub_type: 杂志文章
doi:10.1128/JVI.31.3.752-760.1979
更新日期:1979-09-01 00:00:00
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