Potassium transport in Langendorff-perfused mouse hearts assessed by 87Rb NMR spectroscopy.

Abstract:

:We studied the fluxes of a potassium congener (Rb(+)) in mouse hearts by (87)Rb MRS at 8.4T. The hearts were loaded with Rb(+) by perfusion with Krebs-Henseleit buffer, in which 50% of K(+) was substituted with Rb(+). We initiated Rb(+) efflux by changing the perfusion medium to Rb(+)-free buffer. Spectra were acquired every 1.85 min, and the kinetics of Rb(+) transport were analyzed by means of monoexponential fits. The rate constants of Rb(+) uptake and efflux were 0.0680 +/- 0.0028 and 0.0510 +/- 0.0051 min(-1), respectively (approximately 30% faster than in the rat heart). The ATP-sensitive potassium channel opener, P-1075 (5 microM), and mitochondrial uncoupler, 2,4-dintrophenol (50 microM), activated Rb(+) efflux from mouse hearts by approximately 35%. The mechanisms responsible for the differences in Rb(+) uptake and efflux under baseline conditions and stimulation, in comparison with rat hearts, are discussed. These data provide a background for studies of cardiac potassium transport in transgenic mouse strains.

journal_name

Magn Reson Med

authors

Jilkina O,Xiang B,Kuzio B,Rendell J,Kupriyanov VV

doi

10.1002/mrm.20450

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

1172-6

issue

5

eissn

0740-3194

issn

1522-2594

journal_volume

53

pub_type

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