Imaging of single human carcinoma cells in vitro using a clinical whole-body magnetic resonance scanner at 3.0 T.

Abstract:

:The purpose of the present study was to examine whether single human carcinoma cells labeled with iron oxide nanoparticles could be detected by magnetic resonance (MR) imaging on a clinical 3-T scanner using a surface coil only. WiDr human colon carcinoma cells were loaded with two kinds of iron oxide nanoparticles differing by coating and size: aminosilan-coated (MagForce) and carboxy-dextran-coated particles (Resovist). The latter were preferred by the colon carcinoma cell line used here and taken up much faster (12 h) than the smaller carboxydextran-coated Resovist (48 h). Labeled single carcinoma cells, distributed in an agarose gel in a monodisperse layer as controlled by light microscopy, became detectable as punctuate signal extinctions when using a small circularly polarized surface coil in conjunction with a T(2)*-weighted GE sequence at 3 T. The threshold for the detectability of labeled colon carcinoma cells ranged at a load of 4-5 mug iron/10(6) cells. Obviating the need for special hardware additions, this study opens a new lane for single-cell tracking on clinical 3-T MR scanners amenable to patient studies.

journal_name

Magn Reson Med

authors

Pinkernelle J,Teichgräber U,Neumann F,Lehmkuhl L,Ricke J,Scholz R,Jordan A,Bruhn H

doi

10.1002/mrm.20455

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

1187-92

issue

5

eissn

0740-3194

issn

1522-2594

journal_volume

53

pub_type

杂志文章
  • Transrectal probe for 1H MRI and 31P MR spectroscopy of the prostate gland.

    abstract::A transrectal probe for 1H imaging and 31P MR spectroscopy of the prostate gland is described. The probe is covered with plastic and consists of an insectional portion containing an rf coil and a handle containing a tuning and matching circuit. Using this transrectal probe, 1H MR images and the first 31P MR spectra of...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章,评审

    doi:10.1002/mrm.1910110209

    authors: Narayan P,Vigneron DB,Jajodia P,Anderson CM,Hedgcock MW,Tanagho EA,James TL

    更新日期:1989-08-01 00:00:00

  • Free-breathing volumetric fat/water separation by combining radial sampling, compressed sensing, and parallel imaging.

    abstract:PURPOSE:Conventional fat/water separation techniques require that patients hold breath during abdominal acquisitions, which often fails and limits the achievable spatial resolution and anatomic coverage. This work presents a novel approach for free-breathing volumetric fat/water separation. METHODS:Multiecho data are ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26392

    authors: Benkert T,Feng L,Sodickson DK,Chandarana H,Block KT

    更新日期:2017-08-01 00:00:00

  • Self-calibrating parallel imaging with automatic coil sensitivity extraction.

    abstract::Calibration of the spatial sensitivity functions of coil arrays is a crucial element in parallel magnetic resonance imaging (PMRI). The most common approach has been to measure coil sensitivities directly using one or more low-resolution images acquired before or after accelerated data acquisition. However, since it i...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.10087

    authors: McKenzie CA,Yeh EN,Ohliger MA,Price MD,Sodickson DK

    更新日期:2002-03-01 00:00:00

  • Real-time dynamic frequency and shim correction for single-voxel magnetic resonance spectroscopy.

    abstract::Subject motion during brain magnetic resonance spectroscopy acquisitions generally reduces the magnetic field (B₀) homogeneity across the volume of interest or voxel. This is the case even if prospective motion correction ensures that the voxel follows the head. We introduce a novel method for rapidly mapping linear v...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24129

    authors: Keating B,Ernst T

    更新日期:2012-11-01 00:00:00

  • Susceptibility contrast imaging of cerebral blood volume: human experience.

    abstract::Magnetic resonance (MR) can offer a unique window on the structure/function relationships in the brain, by utilizing the established link between tissue function, metabolism, and hemodynamics. This report focuses on recent applications of MR-based cerebral blood volume (CBV) imaging in humans. Our methodology uses hig...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910220227

    authors: Rosen BR,Belliveau JW,Aronen HJ,Kennedy D,Buchbinder BR,Fischman A,Gruber M,Glas J,Weisskoff RM,Cohen MS

    更新日期:1991-12-01 00:00:00

  • Uncovering of intracellular water in cultured cells.

    abstract::The complexity of biologic tissues, with multiple compartments each with its own diffusion and relaxation properties, requires complex formalisms to model water signal in most magnetic resonance imaging or magnetic resonance spectroscopy experiments. In this article, we describe a magnetic susceptibility-induced shift...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.20565

    authors: Galons JP,Lope-Piedrafita S,Divijak JL,Corum C,Gillies RJ,Trouard TP

    更新日期:2005-07-01 00:00:00

  • Image contrast using the secondary and tertiary eigenvectors in diffusion tensor imaging.

    abstract::Diffusion tensor imaging (DTI) is a new imaging modality that can provide unique information on brain white matter anatomy. Measurements of water diffusion constant along multiple axes are fitted to a tensor model, from which the diffusion anisotropy and dominant fiber orientation can be estimated. Even though the ten...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.20767

    authors: Zhang J,van Zijl PC,Mori S

    更新日期:2006-02-01 00:00:00

  • Application of phase rotation to STRESS localization scheme at 3 T.

    abstract:PURPOSE:Application of phase rotation to the STRESS (=STEAM+PRESS) localization scheme, to shorten echo time, minimize J-coupling dephasing and estimate B1+ inhomogeneity. STRESS (=STEAM + PRESS) simultaneously refocuses and acquires the double spin echo (SE123 ) and stimulated echo (STE- ) pathways, combining PRESS-li...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26911

    authors: Volovyk O,Tal A

    更新日期:2018-05-01 00:00:00

  • Cardiac perfusion imaging using hyperpolarized (13)C urea using flow sensitizing gradients.

    abstract:PURPOSE:To demonstrate the feasibility of imaging the first passage of a bolus of hyperpolarized (13)C urea through the rodent heart using flow-sensitizing gradients to reduce signal from the blood pool. METHODS:A flow-sensitizing bipolar gradient was optimized to reduce the bright signal within the cardiac chambers, ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.25713

    authors: Lau AZ,Miller JJ,Robson MD,Tyler DJ

    更新日期:2016-04-01 00:00:00

  • Rapid acquisition of helium-3 and proton three-dimensional image sets of the human lung in a single breath-hold using compressed sensing.

    abstract:PURPOSE:To develop and validate a method for acquiring helium-3 ((3) He) and proton ((1) H) three-dimensional (3D) image sets of the human lung with isotropic spatial resolution within a 10-s breath-hold by using compressed sensing (CS) acceleration, and to assess the fidelity of undersampled images compared with fully...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.25499

    authors: Qing K,Altes TA,Tustison NJ,Feng X,Chen X,Mata JF,Miller GW,de Lange EE,Tobias WA,Cates GD Jr,Brookeman JR,Mugler JP 3rd

    更新日期:2015-10-01 00:00:00

  • 3D-multi-spectral T2 mapping near metal implants.

    abstract:PURPOSE:Due to host-mediated adverse reaction to metallic debris, there is an increasing need for noninvasive assessment of the soft tissue surrounding large joint arthroplasties. Quantitative T 2 ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.27744

    authors: Bhave S,Koff MF,Sivaram Kaushik S,Potter HG,Koch KM

    更新日期:2019-08-01 00:00:00

  • The analytic theory, optimization, and performance of transparent pulses.

    abstract::Transparent pulses are defined by the property of having no net effect on stationary spins, while selectively nutating and dephasing flowing spins. They are derived from the inverse scattering transform, a nonlinear extension of the inverse Fourier transform. They can be used as presaturation pulses to suppress select...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910240212

    authors: Buonocore MH

    更新日期:1992-04-01 00:00:00

  • The MR tomograph as a sound generator: fMRI tool for the investigation of the auditory cortex.

    abstract::An automatic fMRI protocol for the investigation of the human auditory cortex has been developed. For the activation of the auditory system, the scanner noise itself is the source of sound. Rapid switching of B0 gradients allows the generation of tones of defined frequencies and amplitude on a conventional scanner wit...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910400621

    authors: Bilecen D,Radü EW,Scheffler K

    更新日期:1998-12-01 00:00:00

  • Theoretical analysis of the effect of imperfect slice profiles on tagging schemes for pulsed arterial spin labeling MRI.

    abstract::Pulsed arterial spin labeling (ASL) techniques provide a noninvasive method of obtaining qualitative and quantitative perfusion images with MRI. ASL techniques employ inversion recovery and/or saturation recovery to induce perfusion weighting, and thus the performance of these techniques is dependent on the slice prof...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1169

    authors: Keilholz-George SD,Knight-Scott J,Berr SS

    更新日期:2001-07-01 00:00:00

  • Parallel transmit capability of various RF transmit elements and arrays at 7T MRI.

    abstract:PURPOSE:In this work, 22 configurations for remote radiofrequency (RF) coil arrays consisting of different transmit element designs for 7 Tesla (T) ultrahigh-field MRI are compared by numerical simulations. METHODS:Investigated transmit RF element types are rectangular loops, micro striplines, micro striplines with me...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26704

    authors: Rietsch SHG,Orzada S,Bitz AK,Gratz M,Ladd ME,Quick HH

    更新日期:2018-02-01 00:00:00

  • Shielded-coaxial-cable coils as receive and transceive array elements for 7T human MRI.

    abstract:PURPOSE:To investigate the use of shielded-coaxial-cable (SCC) coils as elements for multi-channel receive-only and transceive arrays for 7T human MRI and to compare their performance with equivalently sized conventional loop coils. METHODS:The SCC coil element consists of a coaxial loop with interrupted central condu...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.27964

    authors: Ruytenberg T,Webb A,Zivkovic I

    更新日期:2020-03-01 00:00:00

  • Perfusion-weighted imaging of interictal hypoperfusion in temporal lobe epilepsy using FAIR-HASTE: comparison with H(2)(15)O PET measurements.

    abstract::To detect perfusion abnormalities in areas of high magnetic susceptibility in the brain, an arterial spin-labeling MRI technique utilizing flow-sensitive alternating inversion recovery (FAIR) and half-Fourier single shot turbo spin-echo (HASTE) for spin preparation and image acquisition, respectively, was developed. I...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/1522-2594(200103)45:3<431::aid-mrm1056>3.0

    authors: Liu HL,Kochunov P,Hou J,Pu Y,Mahankali S,Feng CM,Yee SH,Wan YL,Fox PT,Gao JH

    更新日期:2001-03-01 00:00:00

  • Efficient sample density estimation by combining gridding and an optimized kernel.

    abstract::The reconstruction of non-Cartesian k-space trajectories often requires the estimation of nonuniform sampling density. Particularly for 3D, this calculation can be computationally expensive. The method proposed in this work combines an iterative algorithm previously proposed by Pipe and Menon (Magn Reson Med 1999;41:1...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.23041

    authors: Zwart NR,Johnson KO,Pipe JG

    更新日期:2012-03-01 00:00:00

  • Localized 7Li MR spectroscopy and spin relaxation in rat brain in vivo.

    abstract::Localized 7Li MR point-resolved spectroscopy (PRESS) was developed as a technique to measure lithium (Li) concentration in rat brain in vivo. Localized 7Li spectra could be obtained at 4.7 T in a 0.7-ml voxel in rat brain over the entire therapeutic range of serum Li for humans. Localized 7Li spin-lattice (T1) and spi...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.20112

    authors: Komoroski RA,Pearce JM

    更新日期:2004-07-01 00:00:00

  • Echo shape analysis and image size adjustment on the level of echoes: improvement of parameter-selective proton images.

    abstract::Parameter-selective imaging for tissue characterization is performed at low magnetic fields (0.24-0.28 T) employing echo trains with a large number of echoes (less than or equal to 50), high-power pulses, and the projection reconstruction method. Preprocessing of the raw echoes is important for the quality of the echo...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910030306

    authors: Staemmler M,Gersonde K

    更新日期:1986-06-01 00:00:00

  • Design of spectral-spatial phase prewinding pulses and their use in small-tip fast recovery steady-state imaging.

    abstract:PURPOSE:Spectrally selective "prewinding" radiofrequency pulses can counteract B0 inhomogeneity in steady-state sequences, but can only prephase a limited range of off-resonance. We propose spectral-spatial small-tip angle prewinding pulses that increase the off-resonance bandwidth that can be successfully prephased by...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.26794

    authors: Williams SN,Nielsen JF,Fessler JA,Noll DC

    更新日期:2018-03-01 00:00:00

  • Rapid in vivo musculoskeletal MR with parallel imaging at 7T.

    abstract::The purpose of this work was to implement autocalibrating GRAPPA-based parallel imaging (PI) for in vivo high-resolution (HR) MRI of cartilage and trabecular bone micro-architecture at 7T and to evaluate its performance based on comparison of MR-derived morphology metrics between accelerated and conventional images an...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.21455

    authors: Banerjee S,Krug R,Carballido-Gamio J,Kelley DA,Xu D,Vigneron DB,Majumdar S

    更新日期:2008-03-01 00:00:00

  • Continuous assessment of relative cerebral blood volume in transient ischemia using steady state susceptibility-contrast MRI.

    abstract::The utility of a noninvasive steady state susceptibility-contrast MRI technique for continuous measurement of relative cerebral blood volume (rCBV) during global transient ischemia and subsequent hyperemia in a feline ischemia model is demonstrated. The measurements were obtained during a 10-min period of occlusion an...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910350207

    authors: Hamberg LM,Boccalini P,Stranjalis G,Hunter GJ,Huang Z,Halpern E,Weisskoff RM,Moskowitz MA,Rosen BR

    更新日期:1996-02-01 00:00:00

  • Hypercarbia-induced changes in cerebral blood volume in the cat: a 1H MRI and intravascular contrast agent study.

    abstract::Cerebral blood volume changes with arterial carbon dioxide were monitored by proton T1-weighted MR images following administration of the intravascular contrast agent Gd-DTPA labeled with human serum albumin. Without MR contrast, no significant image intensity changes were observed with PaCO2. Following contrast, regi...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910230104

    authors: Moseley ME,Chew WM,White DL,Kucharczyk J,Litt L,Derugin N,Dupon J,Brasch RC,Norman D

    更新日期:1992-01-01 00:00:00

  • In vivo validation of MR pulse pressure measurement in an aortic flow model: preliminary results.

    abstract::MR imaging experiments were conducted to investigate the feasibility of estimating vascular pulse pressure waveforms from measurements of blood flow rates and vessel cross-sectional area. Blood flow waveforms were measured in the aorta's of three 25-30-kg pigs at multiple imaging sections using phase-contrast velocity...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910380209

    authors: Urchuk SN,Fremes SE,Plewes DB

    更新日期:1997-08-01 00:00:00

  • A fast, analytically based method to optimize local transmit efficiency for a transmit array.

    abstract:PURPOSE:To develop an analytically based algorithm for rapid optimization of the local radiofrequency magnetic (B1+) field intensity for a given radiofrequency power through a transmit array. The analytical nature of the method will yield insight to optimization requirements and provides a valuable reference for numeri...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24653

    authors: Carluccio G,Collins CM,Erricolo D

    更新日期:2014-01-01 00:00:00

  • T2 of endotoxin lung injury with and without methylprednisolone treatment.

    abstract::NMR relaxation times (T1 and T2) and the water content (WC) of in vitro rat lungs were measured during the course of endotoxin lung injury in rats. Measurements of normal lungs, untreated endotoxin-injured lungs, and endotoxin-injured lungs treated with methylprednisolone (MPSL) were compared. The untreated endotoxin ...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910150204

    authors: Shioya S,Haida M,Tsuji C,Ono Y,Miyairi A,Fukuzaki M,Ohta Y,Yamabayashi H

    更新日期:1990-08-01 00:00:00

  • Time-resolved flow measurement in the isolated rat heart: characterization of left coronary artery stenosis.

    abstract::The investigation of flow behavior in coronary arteries is of great importance for an understanding of heart failure and heart regulation mechanisms. The purpose of the present study was to demonstrate that flow velocity can be quantified in the coronary arteries of the isolated rat heart with high-resolution phase co...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.10563

    authors: Köhler S,Hiller KH,Jakob PM,Bauer WR,Haase A

    更新日期:2003-09-01 00:00:00

  • Phase-labeled reference EPI for frequency-segmented inhomogeneity corrections (PREFICS).

    abstract:PURPOSE:To develop a method to correct for geometric distortions in non-Cartesian echo planar imaging using data acquired in a fast prescan. METHODS:Echo planar images were obtained using a non-Cartesian density weighted k-space trajectory. In contrast to linear Cartesian acquisition, distortions arising in non-Cartes...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.24737

    authors: Zeller M,Müller A,Hahn D,Köstler H

    更新日期:2014-03-01 00:00:00

  • Experimental confirmation of phase encoding of instantaneous derivatives of position.

    abstract::Previous theoretical reports described the dependence of interpretation of the observed phase of the NMR signal on the time origin(s) of moment calculations and position's Taylor series expansion. This work provides experimental confirmation of predictions derived from that theory. For accelerative motion, experimenta...

    journal_title:Magnetic resonance in medicine

    pub_type: 杂志文章

    doi:10.1002/mrm.1910320111

    authors: Duerk JL,Simonetti OP,Hurst GC,Finelli DA

    更新日期:1994-07-01 00:00:00