Abstract：
:The pigeon crop-sac is a well-known target organ of prolactin (PRL). Pukac and Horseman (Endocrinology 114: 1718, 1984) reported that injections of the hormone caused changes in the expression of several specific proteins in that organ. We investigated the possibility that measuring the amounts of three of these proteins might improve the precision of the local pigeon crop-sac assay for PRL. Pigeons were given four injections of phosphate-buffered saline or different doses of ovine (o) PRL for 2 days and were killed on day 3. The DNA and total protein content of their crop-sac mucosal epithelial cells were measured and a supernatant of homogenized epithelial cells was processed by SDS-PAGE. The amounts of the three specific proteins in the stained gels were measured by densitometry. Treatment with oPRL increased the concentration of two of these proteins in the crop mucosal tissue (CP 14 and CP 25) and decreased that of the third one (CP 17). Measurement of total protein or of changes in the individual protein bands gave dose-response relationships with poor indices of precision (lambda greater than 0.3). Measurement of DNA content gave an assay endpoint with a reasonably good lambda value (0.16) but when the changes in the amounts of CP 14 and CP 25 were expressed as ratios to the changes occurring in CP 17, very precise assay endpoints (lambda = 0.06-0.08) were obtained.
journal_name
Life Scijournal_title
Life sciencesauthors
Lebovic DI,Nicoll CSdoi
10.1016/0024-3205(92)90532-tsubject
Has Abstractpub_date
1992-01-01 00:00:00pages
2019-24issue
25eissn
0024-3205issn
1879-0631pii
0024-3205(92)90532-Tjournal_volume
50pub_type
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