当前位置： SCI文献检索 > LIFE SCIENCES期刊下所有文献 > KDM4A promotes the growth of non-small cell lung cancer by mediating the expression of Myc via DLX5 through the Wnt/β-catenin signaling pathway.

KDM4A promotes the growth of non-small cell lung cancer by mediating the expression of Myc via DLX5 through the Wnt/β-catenin signaling pathway.

Abstract：

AIMS:Lung cancer represents as the most prevalent tumor globally, among which non-small cell lung cancer (NSCLC) takes up for more than 80%. Lysine demethylase 4A (KDM4A) was dysregulated in NSCLC, but its role on the tumorigenesis in NSCLC cells and the mechanisms of actions are unclear. In this work, we evaluated its connection with NSCLC prognosis, cell proliferation, migration and invasion. MATERIALS AND METHODS:The cancer tissues and adjacent tissues of NSCLC patients were collected, and differentially expressed genes were sequenced by transcriptome. Subsequently, cell growth, apoptosis and metastasis were detected after overexpression or knockdown of KDM4A in NSCLC cell lines. Subsequently, changes in distal-less homeobox 5 (DLX5) promoter histone methylation levels were further detected by ChIP-qPCR, and genes that might be regulated by DLX5 were predicted through the JASPAR website. Finally, the effects of DLX5, Myc and Wnt/β-catenin signaling on the growth, apoptosis and metastasis of NSCLC were investigated by functional rescue experiments. KEY FINDINGS:KDM4A was significantly highly expressed in NSCLC patients and cell lines. Knockdown of KDM4A suppressed the growth and metastasis of NSCLC cell lines in vitro and in vivo, and promoted apoptosis. ChIP-qPCR results demonstrated that KDM4A increased DLX5 transcriptional activity by promoting the demethylation of DLX5. Moreover, DLX5 activated the expression of an oncogene Myc and the downstream Wnt/β-catenin signaling, thereby promoting the occurrence, metastasis and growth of NSCLC. SIGNIFICANCE:Our studies indicated that KDM4A is involved in NSCLC progression via the DLX5/Myc/Wnt/β-catenin axis, which might serve as a potential prognostic marker.

journal_name

Life Sci

journal_title

Life sciences

authors

Sun S,Yang F,Zhu Y,Zhang S

doi

10.1016/j.lfs.2020.118508

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

118508

eissn

0024-3205

issn

1879-0631

pii

S0024-3205(20)31261-3

journal_volume

262

pub_type

杂志文章

相关文献

LIFE SCIENCES文献大全
  • Cardamonin suppresses nitric oxide production via blocking the IFN-γ/STAT pathway in endotoxin-challenged peritoneal macrophages of ICR mice.

    abstract：AIMS:Overproduction of nitric oxide (NO) from inducible NO synthase (iNOS) plays many pivotal roles in various inflammatory diseases. In this study, we examined the effects of 6 flavonoids on lipopolysaccharide (LPS)-induced NO generation in macrophage (Mφ), and addressed molecular mechanisms of cardamonin. MAIN METHO...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2011.06.027

    authors： Takahashi A,Yamamoto N,Murakami A

    更新日期：2011-08-29 00:00:00

  • Dopamine and norepinephrine in the alimentary tract changes after chemical sympathectomy and surgical vagotomy.

    abstract：:The aim of this study was to examine the distribution of dopamine and norepinephrine in the proximal alimentary tract of the rat and to assess the contributions of sympathetic and vagal fibers to the tissue concentrations of both catecholamines. Tissues were extracted in perchloric acid and the catecholamines were sep...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(85)90365-0

    authors： Orloff LA,Orloff MS,Bunnett NW,Walsh JH

    更新日期：1985-04-29 00:00:00

  • Stereospecific effects of d- and l-pentazocine on contractions of the mouse vas deferens.

    abstract：:The effects of the d- and l-isomers of pentazocine were compared to that of racemic pentazocine on contractions of the mouse isolated vas deferens. L-pentazocine inhibited electrically evoked contractions of the mouse vas deferens (MVD) in a dose-dependent manner (ID50 0.37 +/- 0.04 microM). In contrast, d-pentazocine...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(86)90446-7

    authors： Kinouchi K,Fukumitsu K,Takezawa J,Maeda S,Enomoto H,Kakuhari J,Yoshiya I,Inoki R

    更新日期：1986-07-14 00:00:00

  • MicroRNA-1 inhibits proliferation of hepatocarcinoma cells by targeting endothelin-1.

    abstract：AIMS:MicroRNA-1 (miR-1) has been demonstrated as a tumor-suppressive miRNA, which shows a down-regulated pattern in several human malignancies including hepatocellular carcinoma (HCC). However, the pathophysiologic roles of miR-1 and their mechanisms in HCC tumorigenesis are still not totally elucidated. MAIN METHODS:...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2012.08.015

    authors： Li D,Yang P,Li H,Cheng P,Zhang L,Wei D,Su X,Peng J,Gao H,Tan Y,Zhao Z,Li Y,Qi Z,Rui Y,Zhang T

    更新日期：2012-10-05 00:00:00

  • Rapid release of multiple hormones from rat pituitaries perifused with recombinant interleukin-1.

    abstract：:Previous studies demonstrated a direct action of interleukin-1 (IL-1) on release of hormones from rat anterior pituitary cells in monolayer culture. To rule out any possibility of a paracrine effect from the elevated hormones in the static monolayer system, and to examine further the dynamics of hormone release elicit...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(89)90211-7

    authors： Beach JE,Smallridge RC,Kinzer CA,Bernton EW,Holaday JW,Fein HG

    更新日期：1989-01-01 00:00:00

  • Toward a chimeric vaccine against multiple isolates of Mycobacteroides - An integrative approach.

    abstract：AIM:Nontuberculous mycobacterial (NTM) infection such as endophthalmitis, dacryocystitis, and canaliculitis are pervasive across the globe and are currently managed by antibiotics. However, the recent cases of Mycobacteroides developing drug resistance reported along with the improper practice of medicine intrigued us ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2020.117541

    authors： Satyam R,Bhardwaj T,Jha NK,Jha SK,Nand P

    更新日期：2020-06-01 00:00:00

  • Role of nitric oxide and endothelium-derived hyperpolarizing factor (EDHF) in the regulation of blood pressure by leptin in lean and obese rats.

    abstract：:We investigated the role of nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF) in hemodynamic action of leptin. The effect of leptin (1 mg/kg i.p.) on systolic blood pressure (SBP) was examined in lean rats and in rats made obese by feeding highly palatable diet for either 1 or 3 months. Separate ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2005.12.041

    authors： Bełtowski J,Wójcicka G,Jamroz-Wiśniewska A

    更新日期：2006-05-30 00:00:00

  • Lysine demethylase 5B suppresses CC chemokine ligand 14 to promote progression of colorectal cancer through the Wnt/β-catenin pathway.

    abstract：AIMS:Epigenetic and genetic alterations are crucial events in the onset and progression of human cancers including colorectal cancer (CRC). This work aims to probe the relevance of lysine demethylase 5B (KDM5B) to the progression of CRC and the possible molecules involved. MATERIALS AND METHODS:KDM5B expression in CRC...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2020.118726

    authors： Yan G,Li S,Yue M,Li C,Kang Z

    更新日期：2021-01-01 00:00:00

  • Role of bradykinin in acid-induced mesenteric hyperemia and duodenal villous damage.

    abstract：:Intestinal mucosal capsaicin-sensitive afferent nerves mediate, in part, the mesenteric hyperemia after intraduodenal acidification. The hyperemia plays a role in protecting the duodenal mucosa against acid damage. We tested the hypothesis that bradykinin contributes to this protective hyperemia. A specific antagonist...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(01)01455-2

    authors： Leung FW,Iwata F,Kao J,Seno K,Itoh M,Leung JW

    更新日期：2002-01-04 00:00:00

  • Serotonergic mediation of fenfluramine discriminative stimuli in fawn-hooded rats.

    abstract：:Fenfluramine, a drug that induces increased synaptic serotonin, was used to train Fawn-Hooded rats in a drug discrimination paradigm. This strain of rats is thought to possess a genetic serotonin storage abnormality. The intent of the study was to see if the Fawn-Hooded rat was similar or dissimilar to the more freque...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：

    authors： Schechter MD

    更新日期：1997-01-01 00:00:00

  • Improved methods for separation of human cytokeratins.

    abstract：:The cytokeratins from human bladder and esophageal epithelia were separated using chromatographic techniques. The cytokeratins were first extracted from fresh autopsy tissue using high and low salt buffers. Urea, 8.0-9.5 M, was used to solubilize the resulting cytokeratin pellet. Imidazole was found to increase the so...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(90)90576-d

    authors： Meiklejohn B,Nagle RB,McDaniel KM,Wilson GS

    更新日期：1990-01-01 00:00:00

  • Vitamin D3 attenuates lipopolysaccharide-induced cognitive impairment in rats by inhibiting inflammation and oxidative stress.

    abstract：AIMS:Vitamin D is a well-known endocrine regulator of calcium/phosphate homeostasis and has been reported as having a wide range of activities that are potentially beneficial for human health. This study aimed to investigate the effects of pretreatment of vitamin D3 (100, 1000, and 10,000 IU/kg) against lipopolysacchar...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2020.117703

    authors： Mokhtari-Zaer A,Hosseini M,Salmani H,Arab Z,Zareian P

    更新日期：2020-07-15 00:00:00

  • Fenofibrate reduces cardiac remodeling and improves cardiac function in a rat model of severe left ventricle volume overload.

    abstract：AIMS:Fenofibrate is a peroxisome proliferator-associated receptor alpha agonist (PPARα) used clinically for the management of dyslipidemia and is a myocardial fatty acid oxidation stimulator. It has also been shown to have cardiac anti-hypertrophic properties but the effects of fenofibrate on the development of eccentr...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2012.10.022

    authors： Dhahri W,Couet J,Roussel É,Drolet MC,Arsenault M

    更新日期：2013-01-17 00:00:00

  • Role of nitric oxide in lower esophageal sphincter relaxation to swallowing.

    abstract：:Studies were performed in the opossum to define the role of the L-arginine-nitric oxide (NO) pathway in lower esophageal sphincter (LES) relaxation to swallowing and vagal stimulation in viv and intramural nerve stimulation in vitro. In vivo, L-NAME, a water soluble NO synthase (NOS) inhibitor, caused antagonism of LE...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(92)90326-k

    authors： Yamato S,Saha JK,Goyal RK

    更新日期：1992-01-01 00:00:00

  • Protective effect of coptisine free base on indomethacin-induced gastric ulcers in rats: Characterization of potential molecular mechanisms.

    abstract：AIMS:The aim of this study was to comparatively investigate the potential gastroprotective effect and underlying mechanisms of coptisine free base (CFB, 8-hydroxy-7, 8-dihydrocoptisine), berberine and lansoprazole against indomethacin-induced gastric ulcer in rats. MATERIALS AND METHODS:CFB (10, 20 and 40mg/kg), berbe...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2017.12.004

    authors： Luo C,Chen H,Wang Y,Lin G,Li C,Tan L,Su Z,Lai X,Xie J,Zeng H

    更新日期：2018-01-15 00:00:00

  • Chlorpromazine exacerbates hepatic insulin sensitivity via attenuating insulin and leptin signaling pathway, while exercise partially reverses the adverse effects.

    abstract：:Investigated in this study are the effects and mechanisms of exercise and chlorpromazine (CPZ), a widely used conventional antipsychotic drug, on the hepatic insulin sensitivity of 90% pancreatectomized (Px) male Sprague-Dawley rats. The Px diabetic rats were provided with 0, 5, or 50 mg CPZ per kg of body weight (No-...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2007.04.001

    authors： Park S,Hong SM,Lee JE,Sung SR

    更新日期：2007-06-06 00:00:00

  • Oridonin elevates sensitivity of ovarian carcinoma cells to cisplatin via suppressing cisplatin-mediated autophagy.

    abstract：BACKGROUND:Cisplatin resistance has been found to contribute to the failure of ovarian carcinoma treatment. Oridonin is a natural en-kaurane tetracyclic diterpenoid compound discovered in Rabdosia rubescene (Henmsl.) Hara. Herein, we tested whether oridonin could exert chemo-sensitization activity on cisplatin-resistan...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2019.116709

    authors： Zhao Y,Xia H

    更新日期：2019-09-15 00:00:00

  • Involvement of histone methylation in macrophage apoptosis and unstable plaque formation in methionine-induced hyperhomocysteinemic ApoE-/- mice.

    abstract：AIMS:Hyperhomocysteinemia (Hhcy) is an independent risk factor of atherosclerosis and promotes unstable plaque formation. Epigenetic mechanisms play an important role in the pathogenesis of atherosclerosis induced by Hhcy. However, the exact mechanism is still undefined. Lesional apoptotic cells and necrotic core forma...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2017.02.003

    authors： Cong G,Yan R,Huang H,Wang K,Yan N,Jin P,Zhang N,Hou J,Chen D,Jia S

    更新日期：2017-03-15 00:00:00

  • Synergistic action of tiazofurin and retinoic acid on differentiation and colony formation of HL-60 leukemia cells.

    abstract：:Tiazofurin and retinoic acid synergistically induced differentiation and inhibited colony formation in HL-60 human promyelocytic leukemia cells in cell culture. The synergism was the result of different mechanisms of action, since the effect of tiazofurin, unlike that of retinoic acid, was prevented by addition of gua...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(90)90087-8

    authors： Yamaji Y,Natsumeda Y,Yamada Y,Irino S,Weber G

    更新日期：1990-01-01 00:00:00

  • Expression of the peroxisome proliferator-activated receptor gene is decreased in experimental alcoholic liver disease.

    abstract：:Peroxisome proliferator-activated receptor (PPAR) and retinoid x receptor (RXR) play important roles in fatty acid metabolism. The present study examined the regulation of retinoic acid receptor (RAR alpha, beta, and gamma), RXR (alpha, beta, and gamma), PPAR, cytochrome P450 2E1 (CYP2E1), catalase, and beta-actin gen...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(94)00953-8

    authors： Wan YJ,Morimoto M,Thurman RG,Bojes HK,French SW

    更新日期：1995-01-01 00:00:00

  • Functional characteristics of cysteinyl-leukotriene receptor subtypes.

    abstract：:Cysteinyl-leukotrienes, i.e. leukotriene (LT) C4, D4 and E4, are inflammatory mediators and potent airway- and vasoconstrictors. Two different cysteinyl-leukotriene receptors, CysLT1 and CysLT2, have been cloned and functionally characterised using potent CysLT1 receptor antagonists and the dual CysLT1/CysLT2 receptor...

    journal_title：Life sciences

    pub_type： 杂志文章,评审

    doi：10.1016/s0024-3205(02)01733-2

    authors： Bäck M

    更新日期：2002-06-28 00:00:00

  • Omega-3 and omega-6 polyunsaturated fatty acids: Dietary sources, metabolism, and significance - A review.

    abstract：:Linoleic acid (LA) (n-6) and α-linolenic acid (ALA) (n-3) are essential fatty acids (EFAs) as they cannot be synthesized by humans or other higher animals. In the human body, these fatty acids (FAs) give rise to arachidonic acid (ARA, n-6), eicosapentaenoic acid (EPA, n-3), and docosahexaenoic acid (DHA, n-3) that pla...

    journal_title：Life sciences

    pub_type： 杂志文章,评审

    doi：10.1016/j.lfs.2018.04.049

    authors： Saini RK,Keum YS

    更新日期：2018-06-15 00:00:00

  • Design and screening of a novel neuropilin-1 targeted penetrating peptide for anti-angiogenic therapy in glioma.

    abstract：AIMS:This study aimed to design and screen a dual functional fusion peptide that could penetrate the blood-brain barrier and target neuropilin 1 (NRP1) overexpressed in vascular endothelial cells for the anti-angiogenesis of glioma treatment. MAIN METHODS:At the cellular level, the in vitro anti-angiogenic activity of...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2021.119113

    authors： Zhao L,Chen H,Lu L,Zhao C,Malichewe CV,Wang L,Guo X,Zhang X

    更新日期：2021-01-27 00:00:00

  • Impairment in protein expression profile of testicular steroid receptor coregulators in male rat offspring perinatally exposed to Bisphenol A.

    abstract：AIMS:Steroid hormones and steroid receptors (SRs) play a crucial role in spermatogenesis. Steroid receptor coregulators are the major determinants of SR functioning, and any alteration in their expression is known to be associated with impaired spermatogenesis. Since Bisphenol A (BPA) exposure leads to an impairment of...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2009.04.005

    authors： Salian S,Doshi T,Vanage G

    更新日期：2009-07-03 00:00:00

  • Effects of felbamate, kynurenic acid derivatives and NMDA antagonists on in vitro kainate-induced epileptiform activity.

    abstract：:The effects of the novel anticonvulsant felbamate, which binds to the 5-7 dichlorokynurenic binding sites, were tested towards the CA1 epileptiform activity induced in rat hippocampal slices by kainic acid. The effects of the kynurenic acid derivatives 7-chlorokynurenic acid and 5-7 dichlorokynurenic acid and of the N...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(96)00251-2

    authors： Domenici MR,Marinelli S,Sagratella S

    更新日期：1996-05-24 00:00:00

  • Glycosylated hemoglobin and fructosamines: does their determination really reflect the glycemic control in diabetic patients?

    abstract：:The present experiment was designed to determine whether scavenging capacity of serum, in addition to glucose level, influences hemoglobin and serum protein glycosylation in non-insulin dependent diabetic patients. For this purpose forty-seven patients homogeneous for age, disease duration, therapy and glyco-metabolic...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(96)00256-1

    authors： Testa R,Testa I,Manfrini S,Bonfigli AR,Piantanelli L,Marra M,Pieri C

    更新日期：1996-01-01 00:00:00

  • Molecular process in acute liver injury and regeneration induced by carbon tetrachloride.

    abstract：:Injection of carbon tetrachloride (CCl4) intraperitoneally into model animals induces acute liver injury mediated by reactive oxygen species (ROS) as normal metabolites in hepatocytes. In this study, the molecular process in this type of liver injury was analyzed from the aspect of liver function and regulatory factor...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2004.02.030

    authors： Taniguchi M,Takeuchi T,Nakatsuka R,Watanabe T,Sato K

    更新日期：2004-08-13 00:00:00

  • Ablation of capsaicin-sensitive afferent nerves affects insulin response during an intravenous glucose tolerance test.

    abstract：:We investigated the role of sensory nerves in glucose tolerance in conscious Wistar rats neonatally treated with neurotoxin capsaicin or vehicle. Intravenous glucose tolerance tests (IVGTT, 150, 300 and 450 mg in 30 min) were performed to measure glucose tolerance, and glucose, insulin and glucagon levels were measure...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2005.03.011

    authors： van de Wall EH,Gram DX,Strubbe JH,Scheurink AJ,Koolhaas JM

    更新日期：2005-07-29 00:00:00

  • Improvement of metabolic parameters and vascular function by metformin in obese non-diabetic rats.

    abstract：AIMS:Metformin is an insulin sensitizing agent with beneficial effects in diabetic patients on glycemic levels and in the cardiovascular system. We examined whether the metabolic changes and the vascular dysfunction in monosodium glutamate-induced obese non-diabetic (MSG) rats might be improved by metformin. MAIN METH...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2011.11.005

    authors： Lobato NS,Filgueira FP,Hagihara GN,Akamine EH,Pariz JR,Tostes RC,Carvalho MH,Fortes ZB

    更新日期：2012-01-30 00:00:00

  • Pharmacological evaluation of ocular beta-adrenoceptors in rabbit by tissue segment binding method.

    abstract：AIMS:This study evaluates ocular (iris, ciliary body and ciliary process) and nonocular (atria and lung) beta-adrenoceptors in rabbit to characterize the plasma membrane beta-adrenoceptors and binding affinities of beta-adrenoceptor antagonists. MAIN METHODS:The tissue segment binding method with a hydrophilic radioli...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2008.11.016

    authors： Horinouchi T,Morishima S,Tanaka Y,Koike K,Miwa S,Muramatsu I

    更新日期：2009-01-30 00:00:00