Abstract：
:We describe several proteins that are components of the rabbit B cell receptor complex. Two proteins (37 kDa and 42 kDa) were found in non-covalent association with IgM expressed on B cells from peripheral blood. These proteins were also immunoprecipitated by anti-B29 (Ig-beta) and anti-mb1 (Ig-alpha) monoclonal antibodies. As in the mouse and human, the IgM associated molecules were found as heteromeric structures with non-reduced apparent molecular weights of approximately 70-75 kDa. On rabbit B cells we also found these proteins in a 100-135 kDa complex which may represent trimeric or tetrameric structures. By Western blot, the 37 kDa protein was identified as rabbit Ig-beta (B29), suggesting that the 42 kDa protein is rabbit Ig-alpha. These data suggest that rabbit IgM is associated with both Ig-alpha/beta and Ig-(alpha beta)2 or alpha beta gamma complexes. When similar immunoprecipitation studies were performed on lysates made from B cells isolated from appendix follicles, we found two additional IgM associated protein complexes containing 34 kDa and 36 kDa proteins.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Fitts MG,Metzger DW,Hendershot LM,Mage RGdoi
10.1016/0161-5890(95)00028-dsubject
Has Abstractpub_date
1995-07-01 00:00:00pages
753-9issue
10eissn
0161-5890issn
1872-9142pii
016158909500028Djournal_volume
32pub_type
杂志文章abstract：:CD55 is a key regulator of complement activation, expressed on most tissues and cells in man and other mammals. In the rat, alternative splicing in the gene encoding CD55 yields GPI-anchored (GPI-CD55) and transmembrane (TM-CD55) forms. Published Northern blot analysis indicated that while GPI-CD55 was broadly express...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.018
更新日期：2007-03-01 00:00:00
abstract：:Bla g 1 is a major allergen from Blatella germanica and one of the primary allergens used to assess cockroach allergen exposure. The epitope of an anti-Bla g 1 scFv was mapped in order to better understand cross reactivity with other group 1 cockroach allergens and patient IgE epitopes. X-ray crystallography was used ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.02.003
更新日期：2014-06-01 00:00:00
abstract：:The collection of epitopes present within the variable regions of the tumor-specific clonal immunoglobulin expressed by B cell lymphomas (idiotype, Id) can serve as a target for active immunotherapy. Traditionally, tumor-derived Id protein is chemically conjugated to the immunogenic foreign carrier protein keyhole lim...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.10.020
更新日期：2009-01-01 00:00:00
abstract：:The C-terminal domain of protein 4.1G was identified to interact with the cytosolic tail of the high affinity IgG receptor, Fc gamma RI, in yeast two-hybrid screens. Proteins of the 4.1 family have previously been found to mediate receptor/cytoskeleton interactions. In the study presented here, we show an alternativel...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.10.024
更新日期：2008-04-01 00:00:00
abstract：:CD20 is an important target for monoclonal antibody therapy of B-cell malignancies and for some autoimmune disorders. Though there is interest in evaluating the induction of active immunity to CD20 in the mouse model, the CD20 extracellular domain (ECD) has significant secondary and tertiary structure which make it di...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.01.026
更新日期：2010-04-01 00:00:00
abstract：:The functional importance of various cell membrane bound molecules was studied and compared in the NK cytotoxicity and CTL activity. LFA-1 and CD2 participate in both killing functions, while CD3 and CD8/CD4 as well as MHC class I molecules are involved only in CTL activity. Nevertheless CD2- and beta 2-microglobulin ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90164-1
更新日期：1986-11-01 00:00:00
abstract：:Inhibition of the catalytic activity of horseradish peroxidase by rabbit antisera was measured using alternate enzyme substrates. The same general inhibition curve patterns were obtained regardless of the electron donor or hydroperoxide used. In all cases typical biphasic inhibition patterns were found and complete in...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90169-4
更新日期：1983-12-01 00:00:00
abstract：:Hepatitis B virus (HBV) infection afflicts over 350 million people worldwide and is a leading cause of hepatitis, cirrhosis and hepatocellular carcinoma. HBV replicates noncytopathically in hepatocytes, and most of the hepatic injury is caused by the immune response to the virus. While most studies focused on the adap...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.09.022
更新日期：2009-12-01 00:00:00
abstract：:Stimulation of cells of the rat basophilic leukemia line RBL-2H3, which are used as a model in biochemical studies of mast cells, by antigen or by the calcium ionophore ionomycin, are known to cause secretion of mediators of inflammation. These stimuli have now been found to cause a decrease in the cells' cytosolic pH...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90142-3
更新日期：1988-11-01 00:00:00
abstract：:The binding specificity of rat alveolar macrophages (AM phi) for sheep erythrocytes (E) coated with gangliosides GM1, GM2, GM3, GD1a, GD1b or GT1b was analyzed in a rosette assay by studying the inhibitory effect of gangliosides, various carbohydrates, IgG, C3b-like C3, and fibronectin in this assay. The uptake of gan...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90163-x
更新日期：1986-11-01 00:00:00
abstract：:Concanavalin A (Con A)section-binding proteins from mouse spleen leukocytes were characterised by two-dimensional electrophoresis of material precipitated, by Con A plus anti-Con A, from lysates of biosynthetically-labelled cells. Although most cell surface (iodinatable) proteins are known to bind Con A, some of the m...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90030-5
更新日期：1983-04-01 00:00:00
abstract：:We have previously used immunoelectron microscopy (IEM) to generate a three-dimensional map of idiotypic (Id) and isotypic epitopes on the Fab arms of HGAC 39 (Roux et al., 1987, Proc. natn. Acad. Sci. U.S.A. 84, 4984-4988), a mouse IgG3 monoclonal antibody (Mab). In this report, we analyse the geometry of the various...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90167-8
更新日期：1994-06-01 00:00:00
abstract：:Previous studies showed that P2 receptors are involved in neutrophil migration via stimulation of chemokine release and by facilitating chemoattractant gradient sensing. Here, we have investigated whether these receptors are involved in LPS-induced neutrophil transendothelial migration (TEM) using a Boyden chamber whe...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.11.020
更新日期：2010-02-01 00:00:00
abstract：:Naturally developing human antibodies to the Epstein-Barr nuclear antigen recognize synthetic peptides containing sequences from the unusual glycine-alanine region of this protein. We tested antibody binding to a series of peptides of from five to 20 amino acids in length. Peptides as small as seven amino acids could ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90114-7
更新日期：1984-11-01 00:00:00
abstract：:We discovered that some bovine antibodies are amongst the largest known to exist due to the presence of an exceptionally long CDR3H (≥49 amino acids) with multiple cysteines that provide a unique knob and stalk structure to the antigen binding site. The large CDR3H size, unlike mouse and human, provides a suitable pla...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.07.014
更新日期：2016-09-01 00:00:00
abstract：:Peptides representing the amino-terminal sequence of protamines HP1 (sequence 1-12) and HP2 (sequence 1-11), the two major nuclear proteins of human sperm, have been synthesized. Rabbits were immunized either with peptide conjugated with a carrier or with free peptide. The resulting antisera were examined for their ca...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90179-n
更新日期：1991-09-01 00:00:00
abstract：:The number of V alpha and V beta sequences of T cell receptors now available allows a meaningful analysis of their variability profiles. Variability plots were derived using a modified form of Wu and Kabat's algorithm: variability is not computed as a proportion of the number of different residues occurring at a posit...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90178-m
更新日期：1991-09-01 00:00:00
abstract：:Although the etiology of rheumatoid arthritis (RA) remains unknown, it has been widely suggested that RA has a genetic background. In humans, a copy number loss of 22q11.2, a region harboring the VPREB1 gene, has been suggested to be associated with several immunologic disorders, but there has been no study on the cop...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.11.009
更新日期：2011-06-01 00:00:00
abstract：:Consumption of fermentable dietary fibres, such as inulin, or administration of helminth products (e.g. Trichuris suis ova) have independently been shown to alleviate inflammation in vivo. We recently found that dietary inulin and T. suis infection in pigs co-operatively suppressed type-1 inflammatory responses in the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.03.006
更新日期：2020-05-01 00:00:00
abstract：:We designed the peptide (C3) mimetic carboxyl-terminus (Cterminus) of capsid protein of porcine circovirus type 2b (PCV2b-1A/1B) inducing humoral immunity and generating hybridomas. The positive reactivity of the mAbs to PCV2 capsid protein was demonstrated by Western blot assay. Those mAbs also showed positive signal...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.02.001
更新日期：2017-05-01 00:00:00
abstract：:HLA-B27 (B27) is strongly associated with the spondyloarthritides. B27 is expressed at the cell surface of antigen presenting cells (APC) both as canonical β2m-associated and non-canonical β2m-free heavy chain (FHC) forms which include B27 dimers (termed B272). B27 FHC forms arise in an endosomal compartment from recy...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2013.05.243
更新日期：2014-01-01 00:00:00
abstract：:Fibrates, which function by binding and activating peroxisome proliferator-activated receptor alpha (PPARalpha), have been used successfully to treat hyperlipidemia and atherosclerosis. Increasing evidence suggests that in addition to their lipid lowering activities these medications also function as immunosuppressive...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.06.006
更新日期：2007-02-01 00:00:00
abstract：:Antigen-stimulated B lymphocytes undergo genetic and phenotypic changes in germinal centers (GCs), including affinity maturation of immunoglobulin (Ig) genes and Ig heavy chain isotype switching. Expression of the Germinal Center Expressed Transcript (GCET) gene is up-regulated in murine GC B cells. The human homolog ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.06.031
更新日期：2004-11-01 00:00:00
abstract：:The pentacyclic triterpene α,β-amyrin has been previously reported as an effective compound in the treatment of several inflammatory conditions. Recent evidence indicates that α,β-amyrin displayed its effects through interaction with the cannabinoid pathway. We assessed the anti-inflammatory effects of the α,β-amyrin ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.01.018
更新日期：2013-07-01 00:00:00
abstract：:Mammals have evolved a special cellular mechanism for killing invading microbes, which is called the phagocytosis. Neutrophils are the first phagocytosing cells that migrate into the site of infection. In these cells, hypochlorite (HOCl) and other hypohalites, generated in the myeloperoxidase (MPO)-hydrogen peroxide (...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.09.017
更新日期：2019-12-01 00:00:00
abstract：:CD9 is a glycoprotein of the transmembrane 4 superfamily (TM4SF) and is involved in various cellular processes. In this study, we describe the isolation of the full-length cDNA encoding for CD9 molecule (daCD9) of red stingray, Dasyatis akajei. This 1252 bp cDNA was isolated from leukocyte cDNA library and contains 68...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.10.005
更新日期：2006-04-01 00:00:00
abstract：:Mannan-binding lectin (MBL) is a plasma protein of the innate immune system with the ability to initiate antimicrobial and inflammatory actions. MBL deficiency is common. More than 10% of the general population may, depending on definition, be classified as MBL deficient, underlining the redundancy of the immune syste...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2005.06.018
更新日期：2006-01-01 00:00:00
abstract：:Rheumatoid arthritis (RA) is a destructive polyarthritis in which synovial-like fibroblasts (SFs) invade and erode cartilage by expressing membrane-anchored type 1 matrix metalloproteinase (MT1-MMP). The mitogen activated protein kinase (MAPK) pathway is activated in RA SFs, but the mechanism of activation is unknown....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.02.001
更新日期：2013-10-01 00:00:00
abstract：:The cell surface co-stimulatory protein CD154 (CD40L) is a target for monoclonal antibody (mAb) inhibitors of T-cell mediated immune diseases. This protein, like most other members of the TNF ligand family, forms homotrimeric complexes on the cell surface and in solution, with a three-fold axis of symmetry. We find th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00045-7
更新日期：2002-09-01 00:00:00
abstract：:The peptide editor HLA-DM (DM) catalyzes the exchange of peptides bound to MHC class II molecules within antigen presenting cells by generating a "peptide-receptive" MHC class II conformation (MHC(receptive)) to which peptides readily bind and rapidly unbind. While recent work has uncovered the determinants of DM reco...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.07.001
更新日期：2009-09-01 00:00:00
