Abstract：
:The frequency and specificity of antibodies to native and denatured collagens were evaluated in systemic sclerosis (SSc) and in primary Raynaud's phenomenon (PRP) by direct and competitive ELISA. Antibodies reactive with denatured collagen type I (CI) were found in 43% of the SSc sera, and anti-CIV and anti-CV in 31%. In PRP, anti-CI, anti-CIV and anti-CV antibodies were detected in 8% of patient sera. Anti-CI, anti-CIV and anti-CV antibodies reacted with determinants expressed on the native as well as on the denatured molecule. Anti-CI and anti-CIV were cross-reactive; a reactivity with CII and a lower one with CV were detected. Anti-CV antibodies also reacted with CI and CII and, in a smaller proportion of cases, with CIV. Anti-collagen antibodies, affinity-purified from blotted collagen IV and V and cyanogen bromide (CBr)-digested CI, displayed the cross-reactivities shown by inhibition studies on sera. Moreover, antibodies eluted from a CBr fragment of CI reacted with the other CBr fragments as well. These data show that one-third of SSc sera contain antibodies that react with epitopes expressed on native as well as on heat-denatured CI, CII, CIV and CV, and therefore have the potential to bind collagens in vivo.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Riente L,Marchini B,Dolcher MP,Puccetti A,Bombardieri S,Migliorini Pdoi
10.1111/j.1365-2249.1995.tb03789.xsubject
Has Abstractpub_date
1995-11-01 00:00:00pages
354-9issue
2eissn
0009-9104issn
1365-2249journal_volume
102pub_type
杂志文章abstract：:Dipeptidyl peptidase IV (DPIV), a T cell enzyme, has been implicated in the regulation of lymphocyte proliferation in response to lectins and allogeneic cells. A sensitive fluorimetric assay has been established for the enzyme and used to investigate DPIV activity, kinetics and the subcellular localization in lymphocy...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:The Km for AMP for 5' nucleotidase was increased in lymphocytes from patients with common variable (CVH) and sex linked (XLH) hypogammaglobulinaemia and from patients with chronic lymphatic leukaemia (CLL): the Vmax of the latter was low. The kinetic constants for cord blood lymphocytes are similar to adults and the K...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：:Patients with functional deficiency of C1-inhibitor (C1-INH) suffer from recurrent acute attacks (AA) of localized oedema associated with activation of the contact system, complement and fibrinolysis. To unravel further the role of coagulation and fibrinolysis in the pathophysiology of C1-INH deficiency, we performed ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2011.04541.x
更新日期：2012-03-01 00:00:00
abstract：:Mycoplasma pneumoniae is an obligate pathogen that causes pneumonia, tracheobronchitis, pharyngitis and asthma in humans. It is well recognized that membrane lipoproteins are immunostimulants exerting as lipopolysaccharides (LPS) and play a crucial role in the pathogenesis of inflammatory responses upon M. pneumoniae ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13510
更新日期：2021-01-01 00:00:00
abstract：:A complement fixing, non-organ specific IgG autoantibody is described in 29 patients. The autoantibody gives a highly characteristic, granular staining of liver cells, proximal kidney tubules and stomach surface epithelium. By studies with various subcellular fractions from rat liver, employing two different technique...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:The CXC chemokines, monokine induced by interferon (IFN)-gamma (MIG) (CXCL9), IFN-gamma-induced protein 10 (IP-10) (CXCL10) and IFN-inducible T cell alpha chemoattractant (I-TAC) (CXCL11), are known to attract CXCR3- (CXCR3A and CXCR3B) T lymphocytes. We investigated MIG, IP-10 and I-TAC mRNAs expression by semi-quant...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12032
更新日期：2013-04-01 00:00:00
abstract：:The interaction of cationic proteins with IgG, IgA and IgM were investigated by solid phase radioimmunoassay. All these immunoglobulins showed avid binding, IgM giving the strongest reaction, followed by IgA and then IgG. Fc fragments of IgG gave binding, but F(ab')2 fragments from the three main Ig classes did not, s...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:Functional analysis of single Toll-like receptors (TLRs) in vivo is necessary to understand how they shape the ocular inflammation involved in uveitis. In this study we explored the role and mechanisms of TLR-2 agonists on the autoreactive T helper type 17 (Th17) response in experimental autoimmune uveitis (EAU). Trea...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12405
更新日期：2014-11-01 00:00:00
abstract：:The effects of 2.5-40 mg/kg nandrolone decanoate (ND) on the development and growth of mononuclear cell infiltrations in the submandibular glands of NZB/W (B/W) mice have been studied using a quantitative histological method. Injections with ND for 9 months, once every 3 weeks, starting at 4 weeks of age, reduced the ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:Ulcerative colitis (UC) and Crohn's disease (CD) are considered to be immunologically mediated disorders that share certain features with murine models of colitis. Whether any of these models are physiologically relevant to the human condition remains controversial. The hypothesis is that increased amounts of antibodi...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2008.03802.x
更新日期：2009-01-01 00:00:00
abstract：:A technique is described which shows how to obtain a suspension of only progressively motile spermatozoa, from semen, by active penetration into an overlying buffer layer. About 60% of the number of motile spermatozoa in the original semen sample can be obtained as an output in the removed buffer layer. With this meth...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:Immunoenzymatic assays were developed for the measurement of antibodies against mycobacterial lipoarabinomannan (LAM), a cell-free proteic extract (CFX) of Mycobacterium leprae, and the 38-kD protein antigen of M. tuberculosis. Sera from 108 leprosy patients, belonging to all clinical-immunological forms of the spectr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00452.x
更新日期：1998-01-01 00:00:00
abstract：:To elucidate the in vivo mechanisms involved in the impairment in pulmonary defence as the result of treatment with glucocorticoids, we established fatal pneumonia with bacteraemia in dexamethasone (DEX)-treated mice by means of an intratracheal challenge of Pseudomonas aeruginosa. An increased neutrophil influx was o...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2001.01656.x
更新日期：2001-11-01 00:00:00
abstract：:The possible role of cytokines in leprosy reactions was investigated by analysing the levels of tumour necrosis factor (TNF) and interleukin-1 (IL-1) in serum samples from 39 leprosy patients, 22 of them presenting either type I (upgrading) or type II (ENL) reactions. Fifty per cent of the patients showed elevated con...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:Cytotoxic T lymphocytes (CTL) and natural killer (NK) cells have a key role in host defence against infectious pathogens, but their response to bacteria is not well characterized. Non-typeable Haemophilus influenzae is a major cause of respiratory tract infection including otitis media, sinusitis, tonsillitis and chro...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2008.03667.x
更新日期：2008-06-01 00:00:00
abstract：:Ten sera from 48 patients with systemic sclerosis were found to be capable of producing cytotoxicity of human umbilical venous and arterial endothelium when co-cultured with peripheral blood mononuclear cells. Fractionation of sera on Ultrogel and the preparation of monomeric IgG by ion exchange and affinity chromatog...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:The role of complement has been studied in the autologous phase of nephrotoxic nephritis (NTN) in rabbits. No reduction in glomerular fibrin deposition, crescent formation or protection from renal failure was observed in either the standard model of NTN when decomplementing doses of cobra venom factor (CVF) were given...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-06-01 00:00:00
abstract：:In this study we demonstrate that IgG present in the sera of patients with primary Sjögren's syndrome (PSS) could bind and activate muscarinic acetylcholine receptors (mAChRs) of rat parotid gland. These antibodies were able to inhibit in a non-competitive manner the binding of 3H-quinuclidinyl benzilate (QNB) to mACh...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1996.42748.x
更新日期：1996-06-01 00:00:00
abstract：:There have been many studies on the mechanisms of internalization of DNA-anti-DNA immune complexes by cells, including the one used for rheumatoid factor-expressing mouse B cells. In parallel, studies on the role of intracellular DNA sensors in the pathogenesis of systemic lupus erythematosus (SLE) have been conducted...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13382
更新日期：2020-02-01 00:00:00
abstract：:Recent experimental data indicate a pathogenic role of complement activation in congestive heart failure (CHF). The aim of this study was to evaluate contact and complement systems activation in patients hospitalized for an acute episode of CHF. Forty-two of 80 consecutive patients admitted at our hospital with confir...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13011
更新日期：2017-11-01 00:00:00
abstract：:Heymann nephritis of the rat has several similarities to human idiopathic membranous glomerulo-nephropathy, and therefore serves as an animal model for study of the human disease. The disease process in the rat is initiated by autoantibodies forming in situ immune deposits on the surface of the glomerular epithelial c...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1993.tb08209.x
更新日期：1993-12-01 00:00:00
abstract：:Recently we showed the in vivo relevance of chemokines in cases of bacterial peritonitis in continuous ambulatory peritoneal dialysis (CAPD) patients. Mesothelial cells, the most numerous cells in the peritoneal cavity, are hypothesized to function as a main source of chemokine production. We investigated the time- an...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00592.x
更新日期：1998-05-01 00:00:00
abstract：:The sera of seven out of eight patients with Herpes gestationis contained a factor which fixed C3 but not detectable amounts of immunoglobulin (Ig) on the basement membrane zone (BMZ) of normal human skin. Using C2-deficient human serum and C4-deficient guinea-pig serum, fixation was shown to be dependent upon activat...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:We have previously demonstrated that immune platelet destruction observed in an AIDS-free HIV-infected patient was associated with the presence of a cross-reactive antibody recognizing both HIV-glycoprotein (gp)120 and platelet gpIIIa (CD61). We have now investigated the presence of such antibodies in other HIV-infect...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1996.917606.x
更新日期：1996-01-01 00:00:00
abstract：:Calf sub-mandibular lymph node lymphocytes and human peripheral blood lymphocytes were oxidized by NaIO4. In liquid cell cultures, both types of cells were transformed with enhanced DNA synthesis. In mixed lymphocyte cultures, untreated human lymphocytes were transformed by irradiated-oxidized human cells. However, un...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:Both CC- and CXC-chemokines are known to be potent leucocyte activators and chemoattractants and play important roles in inflammatory responses. However, chemokine response to bacillus Calmette-Guérin (BCG) infection remains incompletely defined. In this study, we investigated human CC- [macrophage-derived chemokine (...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2006.03288.x
更新日期：2007-02-01 00:00:00
abstract：:A serial prospective study of cell-mediated immunity to rabbit liver-specific lipoprotein (RLSP) has been done in 26 patients with acute viral hepatitis (AH) (18 HBsAg+ and eight HBsAg-) using a lymphocyte transformation test. An increased stimulation index was recorded in 56% of HBsAg+ cases and in 63% of the HBsAg- ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
abstract：:Epigenetic studies reveal how our genes (nature) are influenced by environment (nurture) leading to wide variability in clinical presentations, especially in autoimmune diseases. Patients with C1-inhibitor deficiency, even within the same family, have diverse clinical presentations that may reflect epigenetic control ...
journal_title：Clinical and experimental immunology
pub_type： 评论,杂志文章
doi：10.1111/cei.13516
更新日期：2020-11-01 00:00:00
abstract：:Histamine (10(-3)-10(-8) M) inhibits PHA-induced proliferation of human peripheral blood lymphocytes (HPBL). Inhibition is detected at low concentrations of PHA but is rarely observed at high PHA concentrations. The histamine type II (H2) receptor agonists dimaprit, impromidine and 4-methylhistamine (4MH) inhibit HPBL...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Compared to normal children, pre-natally infected infants had significantly elevated IgM concentrations throughout the first year of life and elevated IgA levels for the first 6 months. In contrast, IgG levels dropped significantly below normal at 3 months post-partum. Infants born without overt disease but with eleva...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
