CXCR3 axis in patients with primary biliary cirrhosis: a possible novel mechanism of the effect of ursodeoxycholic acid.


:The CXC chemokines, monokine induced by interferon (IFN)-gamma (MIG) (CXCL9), IFN-gamma-induced protein 10 (IP-10) (CXCL10) and IFN-inducible T cell alpha chemoattractant (I-TAC) (CXCL11), are known to attract CXCR3- (CXCR3A and CXCR3B) T lymphocytes. We investigated MIG, IP-10 and I-TAC mRNAs expression by semi-quantitative multiplex reverse transcription-polymerase chain reaction (RT-PCR) in liver biopsies obtained from patients with a first diagnosis of primary biliary cirrhosis [(PBC) = 20] compared to patients with normal liver biopsy [normal controls (NCs) = 20]. Chemokine production was assessed by enzyme-linked immunosorbent assay (ELISA) in serum. Measurements were repeated 6 months after ursodeoxycholic acid (UDCA) treatment in PBC patients. CXCR3A and CXCR3B mRNAs expression was examined in immunomagnetically sorted CD3(+) peripheral blood lymphocytes (PBL) pre- and post-treatment by RT-PCR. Flow cytometry was used to evaluate the expression of CXCR3(+) PBLs of NCs and PBC patients. A marked mRNA expression of MIG and IP-10 was found in PBC patients. I-TAC mRNA was not detected. In serum of PBC patients there was a significant increase of MIG and IP-10 compared to NCs. Interestingly, there was a significant reduction of these proteins in patients' serum after UDCA treatment. I-TAC was not statistically different between groups. CXCR3A mRNA expression was found in PBLs from PBC patients as well as in NCs. CXCR3B mRNA was expressed in four of 20 (19%) NCs and 20 of 20 PBC patients. Flow cytometry revealed a significantly lower CXCR3 expression in NCs (13·5%) than in PBC (37·2%), which was reduced (28·1%, P < 0·01) after UDCA administration. These data suggest a possible role for CXCR3-binding chemokines and their receptor in the aetiopathogenetic recruitment of lymphocytes in PBC and a new mechanism of action for UDCA.


Clin Exp Immunol


Manousou P,Kolios G,Drygiannakis I,Koulentaki M,Pyrovolaki K,Voumvouraki A,Notas G,Bourikas L,Papadaki HA,Kouroumalis E




Has Abstract


2013-04-01 00:00:00












  • Unresponsiveness to Con A in spleen cell cultures of M. lepraemurium-infected mice is dependent on a defective expression of high-affinity IL-2 receptors rather than on a lack of IL-2 production.

    abstract::The production of interleukin-2 (IL-2) by Con A-activated spleen cells (SC) progressively declined and reached negligible values during the course of infection of C57BL/6 mice with Mycobacterium lepraemurium. In addition, the capacity of cultured SC to utilize IL-2 was highly reduced, as demonstrated by the accumulati...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Turcotte R,Lemieux S

    更新日期:1989-04-01 00:00:00

  • Low mannose-binding lectin (MBL) levels in neonates with pneumonia and sepsis.

    abstract::We investigated whether deficiency of mannose-binding lectin (MBL), a component of innate immunity, is associated with neonatal pneumonia and sepsis during the first 72 h, i.e. early onset, and during the first month after birth. In 88 neonatal intensive care patients (71 premature), MBL2 genotype and MBL plasma level...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Frakking FN,Brouwer N,van Eijkelenburg NK,Merkus MP,Kuijpers TW,Offringa M,Dolman KM

    更新日期:2007-11-01 00:00:00

  • Ultrastructural and serological studies on the resistance of activated B cells to the cytotoxic effects of anti-immunoglobulin serum. Patch and cap formation of surface immunoglobulin on mitotic B lymphocytes.

    abstract::Previous studies have shown that rabbit anti-mouse immunoglobulin sera (anti-Ig) which kill non-activated B lymphocytes in the presence of complement, are incapable of doing so when the cells are activated by antigen or mitogen into mitosis. Results reported here indicate that the resistance is not dependent on either...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kerbel RS,Birbeck MS,Robertson D,Cartwright P

    更新日期:1975-04-01 00:00:00

  • Natural killer (NK) T cells are significantly decreased in the peripheral blood of patients with rheumatoid arthritis (RA).

    abstract::The number of NK T cells was measured in relation to the Th1/Th2 imbalance observed in RA. Peripheral blood samples of patients with RA (n = 60) and healthy controls (n = 36) were stained with anti-NK receptor 1A (anti-NKR-P1A), anti-CD56, and anti-CD3 MoAbs, and examined by three-colour flow cytometry. NK T (NKR-P1A+...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Yanagihara Y,Shiozawa K,Takai M,Kyogoku M,Shiozawa S

    更新日期:1999-10-01 00:00:00

  • Demonstration of increased anti-mycobacterial activity in peripheral blood monocytes after BCG vaccination in British school children.

    abstract::A blood sample was taken from children aged 13-15 years immediately before BCG vaccination and 8 weeks after. The children were tuberculin skin-test negative to PPD-S before vaccination and positive after. Mononuclear cells were separated from the blood, infected with Mycobacterium microti at a low bacterium/monocyte ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Cheng SH,Walker L,Poole J,Aber VR,Walker KB,Mitchison DA,Lowrie DB

    更新日期:1988-10-01 00:00:00

  • Circulating carcinoembryonic antigen immune complexes in sera of patients with carcinomata of the gastrointestinal tract.

    abstract::The sera of patients with histologically proven carcinomata of the gastrointestinal tract were fractionated by gel filtration and the fractions assayed for the presence of free carcinoembryonic antigen (CEA) binding immunoglobulins and CEA immune complexes by radioimmuno-double-diffusion, using 125I-CEA as a marker. I...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kapsopoulou-Dominos K,Anderer FA

    更新日期:1979-02-01 00:00:00

  • Antisera to human IgE can also recognize IgA and IgM molecules as produced by selected B lymphoblastoid cell lines.

    abstract::A series of polyclonal and monoclonal anti-human IgE sera reacted with a panel of 11 selected IgA and IgM producing B lymphoblastoid cell lines (BLCLs) in cytoplasmic fluorescence, ELISA and immunoprecipitation assays. The reactions were discordant between the BLCLs and the individual antisera. The panel of 11 BLCLs w...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Hendriks RW,Thompson A,Kraakman ME,Drayer AL,Schuurman RK

    更新日期:1988-11-01 00:00:00

  • Differential responses of human dendritic cells to metabolites from the oral/airway microbiome.

    abstract::Small molecule metabolites that are produced or altered by host-associated microbial communities are emerging as significant immune response modifiers. However, there is a key gap in our knowledge of how oral microbial metabolites affect the immune response. Here, we examined the effects of metabolites from five bacte...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Whiteson K,Agrawal S,Agrawal A

    更新日期:2017-06-01 00:00:00

  • Comparison of the immune response against Mycobacterium tuberculosis antigens between a group of patients with active pulmonary tuberculosis and healthy household contacts.

    abstract::The mycobacterial antigens and the factors related to protection for the development of active tuberculosis are not known. In a natural model of tuberculosis, we studied 10 patients with active pulmonary tuberculosis (non-protective immune response) and 38 healthy household contacts (protective immune response). We te...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Torres M,Mendez-Sampeiro P,Jimenez-Zamudio L,Teran L,Camarena A,Quezada R,Ramos E,Sada E

    更新日期:1994-04-01 00:00:00

  • Immune responses to fractionated cytomegalovirus (CMV) antigens after HIV infection. Loss of cellular and humoral reactivity to antigens recognized by HIV-, CMV+ individuals.

    abstract::In order to delineate the molecular pathogenesis of the increased susceptibility to CMV disease in HIV infection, the patterns of antigen responsiveness in HIV-infected and non-infected individuals were investigated. CMV was fractionated by SDS-PAGE and electroblotted onto nitrocellulose. Lymphoproliferative responses...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Converse PJ,Fehniger TE,Ehrnst A,Strannegård O,Britton S

    更新日期:1990-12-01 00:00:00

  • Difference in the cell proliferation and colony-forming ability of normal human T lymphocytes.

    abstract::Cell transfer experiments were carried out with phytohaemagglutinin-induced normal human T lymphocyte colonies after 2--10 days of primary colony growth. The cells gave a cloning efficiency of 15% after 2 days of incubation and this decreased to 0.2% with the progressive growth of the colonies. The primary colonies ha...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Smith SD,Sachs L

    更新日期:1979-08-01 00:00:00

  • The influence of prednisolone on the recirculation of peripheral blood lymphocytes in vivo.

    abstract::In humans and in laboratory animals, administration of a single does of glucocorticosteroids induces a rapid and transient decrease in the numbers of peripheral blood lymphocytes through a redistribution of circulating lymphocytes. This lymphocytopenic effect is an important factor in the immunosuppressive capacity of...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Bloemena E,Weinreich S,Schellekens PT

    更新日期:1990-06-01 00:00:00

  • Localization of 20-kD homologous restriction factor (HRF20) in diseased human glomeruli. An immunofluorescence study.

    abstract::The 20-kD homologous restriction factor (HRF20), which is identical to CD59, is a membrane-associated protein which inhibits the reaction of C9 to form membrane attack complex (MAC) of homologous complements. In various human glomerular diseases deposition of complement components is frequently seen and MAC is reporte...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Tamai H,Matsuo S,Fukatsu A,Nishikawa K,Sakamoto N,Yoshioka K,Okada N,Okada H

    更新日期:1991-05-01 00:00:00

  • Selective CCR2-targeted macrophage depletion ameliorates experimental mesangioproliferative glomerulonephritis.

    abstract::The CCL2/CCR2 chemokine/receptor axis directs the chemotaxis of infiltrating monocytes/macrophages and T cells and plays a pivotal role in tissue damage and fibrosis in kidney diseases. The eradication of the activated leucocytes should diminish the production of inflammatory mediators, limit tissue damage and amelior...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: McIntosh LM,Barnes JL,Barnes VL,McDonald JR

    更新日期:2009-02-01 00:00:00

  • Low immune responses in high androgen responder C57BR/cdj males.

    abstract::Previous studies have shown that male mice of strains with high target organ responsiveness to androgen, the C57L/J and RF/J, have lower plasma immunoglobulin levels and weaker antibody responses to protein and polysaccharide antigens than mice of low androgen responder (LAR) strains, the A/J and 129/J. In the current...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Cohn DA

    更新日期:1986-01-01 00:00:00

  • Autoantibodies in sera of Thai patients with Plasmodium falciparum infection.

    abstract::Serum from 98 Thai adults infected with Plasmodium falciparum were examined for the presence of autoantibodies. Malarial containing antibodies sera were also revealed positive for anti-nuclear antibodies with fluorescence speckled pattern, anti-smooth muscle antibodies, anti-mitochondria antibodies and rheumatoid fact...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Boonpucknavig S,Ekapanyakul G

    更新日期:1984-10-01 00:00:00

  • Piroxicam, indomethacin and aspirin action on a murine fibrosarcoma. Effects on tumour-associated and peritoneal macrophages.

    abstract::Growth of a methylcholanthrene-induced fibrosarcoma in BALB/c mice was accompanied by an increase in the activation state of tumour-associated macrophages (TAM), as measured by their FcIgG receptor expression, phagocytic index and beta-glucuronidase levels. All of these parameters were markedly higher in TAM than in p...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Valdéz JC,Perdigón G

    更新日期:1991-11-01 00:00:00

  • The effect of cortisone acetate on pulmonary lesions in a rabbit model of hypersensitivity pneumonitis (cortisone and hypersensitivity pneumonitis).

    abstract::Rabbits were sensitized with Micropolyspora faeni by intratracheal inoculations and later challenged with the same antigen either with or without parenteral administration of cortisone acetate prior to challenge. Animals developed anti-M. faeni serum precipitins, M. faeni-induced alveolar macrophage migration inhibiti...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Cashner FM,Schuyler MR,Salvaggio JE

    更新日期:1979-05-01 00:00:00

  • Interactions between the mannose receptor and thyroid autoantigens.

    abstract::Thyroid autoantigens require internalization and processing by antigen-presenting cells to induce immune responses. Besides pinocytosis, antigen uptake can be receptor-mediated. The mannose receptor (ManR) has a cysteine rich domain (CR) and eight carbohydrate recognition domains (CRD) that bind glycosylated proteins....

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Chazenbalk GD,Pichurin PN,Guo J,Rapoport B,McLachlan SM

    更新日期:2005-02-01 00:00:00

  • T cell subpopulations in CLL: methods of T cell enrichment artificially alter proportions of OKT4 and OKT8 positive cells.

    abstract::There is growing speculation about the meaning of reported imbalances in subpopulations of T lymphocytes in patients with chronic lymphocytic leukaemia (CLL). This study compared two techniques for producing T cell enriched subpopulations from both patients with CLL and normal individuals and the effects of these tech...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Dickinson AM,George S,Proctor SJ

    更新日期:1983-11-01 00:00:00

  • Successful transfer of collagen-induced arthritis to severe combined immunodeficient (SCID) mice.

    abstract::We describe the adoptive transfer of erosive arthritis to an immunodeficient host. Spleen cells from arthritic DBA/1 mice (H-2q), immunized 4-6 weeks previously with bovine type II collagen in adjuvant, were transferred intraperitoneally into SCID mice (H-2d). SCID recipient mice also received native or denatured type...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Williams RO,Plater-Zyberk C,Williams DG,Maini RN

    更新日期:1992-06-01 00:00:00

  • Monocyte IgG-Fc receptors in myotonic dystrophy.

    abstract::Myotonic dystrophy (MyD), an autosomal dominant neuromuscular disease with multisystem abnormalities, is associated with hypercatabolism of IgG. The hypercatabolism is not related to structural abnormalities of the IgG molecule in MyD but appears to be due to a derangement of the serum IgG concentration-fractional cat...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Banerjee D,McClintock J,Silver MM,Hudson AJ

    更新日期:1982-12-01 00:00:00

  • The chemokine network. II. On how polymorphisms and alternative splicing increase the number of molecular species and configure intricate patterns of disease susceptibility.

    abstract::In this second review on chemokines, we focus on the polymorphisms and alternative splicings and on their consequences in disease. Because chemokines are key mediators in the pathogenesis of inflammatory, autoimmune, vascular and neoplastic disorders, a large number of studies attempting to relate particular polymorph...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Colobran R,Pujol-Borrell R,Armengol MP,Juan M

    更新日期:2007-10-01 00:00:00

  • Specificity and immunochemical properties of antibodies to bacterial DNA in sera of normal human subjects and patients with systemic lupus erythematosus (SLE).

    abstract::To elucidate the mechanisms of anti-DNA production, we assessed the binding of sera of normal human subjects (NHS) and patients with SLE to a panel of bacterial and mammalian DNA. Using single-stranded DNA as antigens in an ELISA, NHS showed significant binding to some but not all bacterial DNA, while lacking reactivi...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Wu ZQ,Drayton D,Pisetsky DS

    更新日期:1997-07-01 00:00:00

  • Trypanosoma cruzi: immunological consequences of parasite modification of host cells.

    abstract::Parasite antigens released from Trypanosoma cruzi-infected cells were adsorbed to infected and uninfected mammalian cells thus rendering them susceptible to immune lysis by antibody and cell-mediated immunity directed against the parasite. BALB/c mice infected with T. cruzi for 15 days developed cytotoxic T lymphocyte...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ribeiro Dos Santos R,Hudson L

    更新日期:1980-04-01 00:00:00

  • Blood levels of transforming growth factor-beta 1 (TGF-beta1) are elevated in both relapsing remitting and chronic progressive multiple sclerosis (MS) patients and are further augmented by treatment with interferon-beta 1b (IFN-beta1b).

    abstract::The serum levels of TGF-beta1, measured by solid-phase ELISA, were determined to be significantly augmented in patients with both relapsing remitting (RR) and secondary chronic progressive (CP) MS compared with sex- and age-matched healthy controls. Moreover, in RR MS patients, the blood levels of the cytokine were fu...

    journal_title:Clinical and experimental immunology

    pub_type: 临床试验,杂志文章


    authors: Nicoletti F,Di Marco R,Patti F,Reggio E,Nicoletti A,Zaccone P,Stivala F,Meroni PL,Reggio A

    更新日期:1998-07-01 00:00:00

  • A preliminary assessment of alpha-1 antitrypsin S and Z deficiency allele frequencies in common variable immunodeficiency patients with and without bronchiectasis.

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    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Sansom ME,Ferry BL,Sherrell ZP,Chapel HM

    更新日期:2002-12-01 00:00:00

  • Epigenetic alterations on C1-inhibitor expression may influence hereditary angioedema attack frequency and C4 levels: Comment on: Karagianni P, Goules AV, Tzioufas AG. Epigenetic alterations in Sjogren's syndrome patient saliva.

    abstract::Epigenetic studies reveal how our genes (nature) are influenced by environment (nurture) leading to wide variability in clinical presentations, especially in autoimmune diseases. Patients with C1-inhibitor deficiency, even within the same family, have diverse clinical presentations that may reflect epigenetic control ...

    journal_title:Clinical and experimental immunology

    pub_type: 评论,杂志文章


    authors: Khan S,Longhurst H

    更新日期:2020-11-01 00:00:00

  • Allergic airway inflammation and susceptibility to pneumococcal pneumonia in a murine model with real-time in vivo evaluation.

    abstract::The relationship between allergic airway inflammation and pneumococcal pneumonia is not well understood. We assessed susceptibility to experimental pneumococcal pneumonia in mice with and without allergic airway inflammation. Susceptibility to pneumococcal pneumonia was evaluated by challenging mice with a bioluminesc...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kang CI,Rouse MS,Patel R,Kita H,Juhn YJ

    更新日期:2009-06-01 00:00:00

  • T cell receptor gamma delta bearing cells are decreased in the peripheral blood of patients with atopic diseases.

    abstract::The biological role of T cell receptor (TCR) gamma delta bearing cells is currently not fully understood. Recently, a monoclonal antibody (TCR delta 1) reacting against the whole molecule became available which facilitates the direct analysis of TCR-gamma delta+ cells. We studied 11 children with atopic dermatitis, 20...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Schauer U,Dippel E,Gieler U,Bräuer J,Jung T,Heymanns J,Rieger CH

    更新日期:1991-12-01 00:00:00